Survival of patients receiving a liver transplant for hepatocellular carcinoma, and risk of tumor recurrence

Objective: the goal of this research has been to evaluate the survival, in long and short term, of the patient receiving liver transplant for hepatocellular carcinoma (HCC), the risk of posttransplant tumor relapse and factors related to this complication. Design: retrospective study of a consecutive series of patients having had liver transplant for HCC. Patients and methodology: transplant patients for HCC from 1989 to November 2003. Patients were selected due to general limitations of nodule size and quantity, which were subsequently published as Milan criteria. Also, criteria agreed in the Conference of Barcelona were followed in the pre-transplant diagnosis. Results: the survival of this 81 patients group was of the 80, 61 and 52% for 1, 5 and 10 years respectively. In the 32% of the cases the HCC was an incidental finding in the explant. In the 12.3%, the tumor relapse was verified. The multivariate research identified the size of the nodule (OR = 1,7944) (IC 95% = 1,1332-2,8413) and the vascular invasion (OR = 6,6346) (IC 95% = 1,4624-30,1003) as risk factors of relapse. Conclusions: the liver transplant in selected patients with HCC has good results in medium and long term. The risk of post-transplant tumor relapse becomes notably reduced and is associated with the size of the nodule and the microscopic vascular invasion

Large scale multifactorial likelihood quantitative analysis of BRCA1 and BRCA2 variants: An ENIGMA resource to support clinical variant classification

The multifactorial likelihood analysis method has demonstrated utility for quantitative assessment of variant pathogenicity for multiple cancer syndrome genes. Independent data types currently incorporated in the model for assessing BRCA1 and BRCA2 variants include clinically calibrated prior probability of pathogenicity based on variant location and bioinformatic prediction of variant effect, co-segregation, family cancer history profile, co-occurrence with a pathogenic variant in the same gene, breast tumor pathology, and case-control information. Research and clinical data for multifactorial likelihood analysis were collated for 1,395 BRCA1/2 predominantly intronic and missense variants, enabling classification based on posterior probability of pathogenicity for 734 variants: 447 variants were classified as (likely) benign, and 94 as (likely) pathogenic; and 248 classifications were new or considerably altered relative to ClinVar submissions. Classifications were compared with information not yet included in the likelihood model, and evidence strengths aligned to those recommended for ACMG/AMP classification codes. Altered mRNA splicing or function relative to known nonpathogenic variant controls were moderately to strongly predictive of variant pathogenicity. Variant absence in population datasets provided supporting evidence for variant pathogenicity. These findings have direct relevance for BRCA1 and BRCA2 variant evaluation, and justify the need for gene-specific calibration of evidence types used for variant classification

Taller de experimentos en el laboratorio

Los objetivos de este proyecto son ampliar y estimular la curiosidad, imaginación y espíritu crítico del niño. Habituarlo a trabajar experimentalmente (observando, describiendo, formulando hipótesis, anticipando resultados, comprobando...).Favorecer el conocimiento del entorno físico y natural. Utilizar la imaginación y la creatividad para descubrir e investigar los fenómenos físicos y naturales. Aplicar los conceptos y estrategias adquiridas a nuevas situaciones que se planteen. Expresar opiniones personales aunque sean en contra del parecer del grupo. Progresar en el desarrollo de sus capacidades cognoscitivas, motrices, de equilibrio personal, de relación e inserción social y autonomía. Aprender a amar la naturaleza y cuidar el medio ambiente en el que desarrollan su vida. La finalidad se centra en aprender a amar la naturaleza y cuidar el medio ambiente. Se estudiará el agua, las plantas, la luz, el magnetismo, los animales, etc. Se realizarán actividades como juegos con linternas, teatrillo de sombras, tren de imanes, construcción de bombas de agua, hacer pulseras de flores, organizar un ensectario, realizar murales, etc..Gobierno de Cantabria. Consejería de Educación y JuventudCantabriaES

Taller de cocina en educación infantil

El taller se llevó a cabo en el CP Mata Linares en San Vicente de la Barquera por cuatro maestras de infantil, los objetivos fueron: adquirir hábitos de higiene y salud personal relacionados con la alimentación. Adquirir hábitos de autonomía personal. Conocer algunos de los utensilios que se utilizan habitualmente en la cocina. Desarrollar el sentido crítico para comer lo adecuado sin dejarse llevar por la publicidad o la moda. Reflexionar y ser conscientes del peligro que entraña manejar algunos aparatos de la cocina sin la presencia de un adulto. Participar activamente en la elaboración de recetas de cocina. Vivenciar el momento de la comida como una situación de diálogo y comunicación familiar y social. Las actividades realizadas para el taller estuvieron orientadas a diferentes áreas: de identidad y autonomía personal (poner la mesa y quitarla, lavarse las manos,etc). Medio físico y social (elaboración de recetas típicas de la comunidad, clasificación de alimentos, utilización de utensilios, etc). Comunicación y representación (Utilización adecuada del vocabulario aprendido, verbalización de todas las situaciones, sensaciones e ideas que se producen en la manipulación de ingredientes y en el proceso de elaboración de recetas). El material utilizado fue: material de cocina, alimentos, material fungible de papelería y del aula, material fotográfico.Gobierno de CantabriaCantabriaES

Macrophage Migration Inhibitory Factor Levels in Gingival Crevicular Fluid, Saliva, and Serum of Chronic Periodontitis Patients

Chronic periodontitis (CP) is an infection that affects the teeth supporting structure. Macrophage migration inhibitory factor (MIF) is an important effector cytokine of the innate immune system. Due to its functional characteristics, MIF may be involved in the immunopathology of CP. The aim of the present study was to evaluate MIF levels in gingival crevicular fluid (GCF), saliva, and serum of CP patients. A cross-sectional study was conducted on 60 subjects divided into two groups: subjects with CP (n= 30) and periodontally healthy subjects without CP (n=30). MIF was quantified in GCF, saliva, and serum of all participants by enzyme-linked immunosorbent assay. MIF concentrations were higher in GCF, saliva, and serum in the group with CP compared with the group without CP and a higher MIF concentration was observed in GCF (p=0.001) and saliva (p=0.009) in the group with CP. MIF intragroup comparisons between fluids demonstrated significant high levels of MIF in saliva compared with GCF and serum in both study groups (p<0.05). A positive correlation was found between clinical signs and MIF concentration in GCF (p<0.05). There is an association between the MIF and the clinical signs of the disease. Therefore, MIF could have an important role in the pathology and progression of CP

Niveles séricos de IL6, IL17 y TNFα en adolescentes del occidente de México

ResumenObjetivo. Comparar concentraciones séricas de IL-6, IL-17 y TNFα en adolescentes con obesidad y dislipidemia del occidente de México.Material y métodos. Estudio transversal analítico. Incluyó adolescentes de 10 a 15 años, ambos sexos, doce con obesidad (IMC±30) y dislipidemia (hipercolesterolemia ≥200mg/dL o hipertrigliceridemia ≥150mg/dL) y 17 con peso saludable (IMC±20), sin enfermedades agudas o crónicas que comprometieran sistema inmune o que tomaran algún medicamento. Se realizaron medidas antropométricas con métodos convencionales estandarizados, se cuantificó colesterol total, triglicéridos, IL-6, IL-17 y TNFα.Resultados. La edad promedio del grupo con obesidad fue de 12 años 3meses (±1.5) y del grupo con peso saludable 13 años 7 meses (± 0.9).  La distribución por género en el grupo con obesidad y dislipidemia mostró que  41.7%  fueron mujeres y 58.3% hombres, mientras que en el grupo de peso saludable hubo 41.2% de mujeres y 58.8% hombres. Se observó diferencia significativa entre grupos en TNFα (247.6 ± 136.4 vs 88.7 ± 48.4pg/mL; p<0.001) no así en los valores de IL-6 (252.1± 127.1 vs 296.3 ± 203pg/mL; p<0.948) e IL-17 (132.3 ± 71.9 vs 205.2 ± 168pg/mL; p <0.195).  El valor sérico del colesterol total en el grupo de obesos fue de 181.2 (±48.3mg/dL; p<0.059) y de triglicéridos 278.7 (±88.6; mg/dL; p<0.000) contrario al grupo control que presentó niveles normales. Conclusiones.  Los pacientes con obesidad y dislipidemia presentan niveles séricos elevados de TNFα (p=0.001) en comparación con el grupo control. No se encontró diferencia estadística en los valores de IL-6 e IL-17 entre grupos

Targets and Effects of Common Biocompounds of <i>Hibiscus sabdariffa</i> (Delphinidin-3-Sambubiosid, Quercetin, and Hibiscus Acid) in Different Pathways of Human Cells According to a Bioinformatic Assay

The utilization of food as a therapeutic measure for various ailments has been a prevalent practice throughout history and across different cultures. This is exemplified in societies where substances like Hibiscus sabdariffa have been employed to manage health conditions like hypertension and elevated blood glucose levels. The inherent bioactive compounds found in this plant, namely, delphinidin-3-sambubioside (DS3), quercetin (QRC), and hibiscus acid (HA), have been linked to various health benefits. Despite receiving individual attention, the specific molecular targets for these compounds remain unclear. In this study, computational analysis was conducted using bioinformatics tools such as Swiss Target Prediction, ShinnyGo 0.77, KEGG, and Stringdb to identify the molecular targets, pathways, and hub genes. Supplementary results were obtained through a thorough literature search in PubMed. DS3 analysis revealed potential genetic alterations related to the metabolism of nitrogen and glucose, inflammation, angiogenesis, and cell proliferation, particularly impacting the PI3K-AKT signaling pathway. QRC analysis demonstrated interconnected targets spanning multiple pathways, with some overlap with DS3 analysis and a particular focus on pathways related to cancer. HA analysis revealed distinct targets, especially those associated with pathways related to the nervous system. These findings emphasize the necessity for focused research on the molecular effects of DS3, QRC, and HA, thereby providing valuable insights into potential therapeutic pathways