Optical manipulation of a single clay nanosheet hybridized with a porphyrin derivative

The effect of hybridization of a clay fluorohectorite (FHT) nanosheet with a π-conjugated organic compound, α,β,γ,δ-tetrakis(1-methylpyridinium-4-yl)porphyrin p-toluene-sulfonate (TMPyP), on its optical manipulation is investigated. Although the hybridized FHT is optically trapped essentially in the same manner as that of neat FHT, the hybridization with TMPyP allows for manipulation of FHT with lower laser intensity or a shorter period, or both. This is ascribed to the larger refractive index and polarizability of TMPyP compared with neat FHT

Radiation-Pressure-Induced Hierarchical Structure of Liquid-Crystalline Inorganic Nanosheets

Although hierarchical assemblies of colloidal particles add novel structure-based functions to systems, few local and on-demand colloidal structures have been developed. We have combined the colloidal liquid crystallinity of two-dimensional inorganic particles and laser radiation pressure to obtain a large hierarchical and local structure in a colloidal system. The scattering force of the laser beam converted the parallel nanosheet alignment to the direction of the incident laser beam. At the focal point, the nanosheet orientation depends on the electric field of the polarized laser beam. In contrast, a giant tree-ring-like nanosheet texture of more than 100 μm, and which is independent of the polarization direction, was organized at the periphery of the focal point. This organization resulted from a cooperative effect between the liquid-crystalline nanosheets, which indicates an effectiveness of optical manipulation to construct hierarchical colloidal structures with the aid of interparticle interactions

Microscope Observation of Morphology of Colloidally Dispersed Niobate Nanosheets Combined with Optical Trapping

Although inorganic nanosheets prepared by exfoliation (delamination) of layered crystals have attracted great attention as 2D nanoparticles, in situ real space observations of exfoliated nanosheets in the colloidally dispersed state have not been conducted. In the present study, colloidally dispersed inorganic nanosheets prepared by exfoliation of layered niobate are directly observed with bright-field optical microscopy, which detects large nanosheets with lateral length larger than several micrometers. The observed nanosheets are not strictly flat but rounded, undulated, or folded in many cases. Optical trapping of nanosheets by laser radiation pressure has clarified their uneven cross-sectional shapes. Their morphology is retained under the relation between Brownian motion and optical trapping

Comprehensive analysis including the nutritional point of view on the pathogenesis of periodontal disease

To clarify risk factors for periodontal disease from the viewpoints of physiology, blood biochemistry, and nutrition, a survey involving 364 persons (224 males, 140 females) who consulted the Medical Examination Center of Matsumoto Dental University Hospital was conducted. The pathogenesis of periodontal disease was investigated using the maximum Community Periodontal Index (CPI) and Attachment Loss (AL) values, and their distributions with respect to the sex were analyzed using Wilcoxonʼs rank sum test. Based on the CPI and AL values, the subjects were divided into 3 groups: healthy (0), mild (1–2), and severe (3–4). The mean values obtained from the physiological, dental, blood biochemical, and nutritional findings in the 3 groups were analyzed using the multiple comparison test. Furthermore, their distributions with respect to sex and smoking in the 3 groups were analyzed using Fisherʼs direct probability test. A p–value of 0.05 was regarded as significant. Factors influencing the CPI included the sex (male), body mass index (BMI), abdominal circumference, diastolic blood pressure, AL, alanine aminotransferase (ALT), fasting blood glucose, neutral fat, HDL cholesterol, and smoking. Factors influencing the AL included the sex (male), age, current number of teeth, CPI, lipid intake, manganese intake, vitamin C intake, monounsaturated fatty acid intake, polyunsaturated fatty acid intake, n–6 fatty acid intake, fruit intake, and smoking. The results suggest that the physiological, blood biochemical, and nutritional states are involved in the pathogenesis of periodontal disease. The CPI was associated with metabolic error in the presence of metabolic syndrome. There was an association between the AL and diet as an environmental factor

Factors related to the number of missing teeth from a physiological, blood biochemical, and nutritional point of view

The aim of this study was to clarify the risk factors for tooth loss from a physiological, blood biochemical, and nutritional point of view. The subjects of this study were 364 people (224 males, 140 females). They were examinees of a medical examination center in Matsumoto Dental University Hospital. Using the number of teeth present (including sound, decayed, and filled teeth) as the response variable, a multiple regression analysis was conducted using parameters in the mouth, physiological parameters, blood biochemical parameters, and nutritional parameters as covariates. In the multiple regression analysis with the response variable as the number of teeth present, the significant influence of attachment loss, sugar–sweetener intake, age, and sodium intake was noted on a decreasing number of teeth in the study subjects. Thus, the number of teeth present was influenced by the physiological, blood biochemical, and nutritional condition. In the future, increasing the number of cases will be necessary along with long–term follow–up

Collaborative Action of Brca1 and CtIP in Elimination of Covalent Modifications from Double-Strand Breaks to Facilitate Subsequent Break Repair

Topoisomerase inhibitors such as camptothecin and etoposide are used as anti-cancer drugs and induce double-strand breaks (DSBs) in genomic DNA in cycling cells. These DSBs are often covalently bound with polypeptides at the 3′ and 5′ ends. Such modifications must be eliminated before DSB repair can take place, but it remains elusive which nucleases are involved in this process. Previous studies show that CtIP plays a critical role in the generation of 3′ single-strand overhang at “clean” DSBs, thus initiating homologous recombination (HR)–dependent DSB repair. To analyze the function of CtIP in detail, we conditionally disrupted the CtIP gene in the chicken DT40 cell line. We found that CtIP is essential for cellular proliferation as well as for the formation of 3′ single-strand overhang, similar to what is observed in DT40 cells deficient in the Mre11/Rad50/Nbs1 complex. We also generated DT40 cell line harboring CtIP with an alanine substitution at residue Ser332, which is required for interaction with BRCA1. Although the resulting CtIPS332A/−/− cells exhibited accumulation of RPA and Rad51 upon DNA damage, and were proficient in HR, they showed a marked hypersensitivity to camptothecin and etoposide in comparison with CtIP+/−/− cells. Finally, CtIPS332A/−/−BRCA1−/− and CtIP+/−/−BRCA1−/− showed similar sensitivities to these reagents. Taken together, our data indicate that, in addition to its function in HR, CtIP plays a role in cellular tolerance to topoisomerase inhibitors. We propose that the BRCA1-CtIP complex plays a role in the nuclease-mediated elimination of oligonucleotides covalently bound to polypeptides from DSBs, thereby facilitating subsequent DSB repair

卵殻破壊強度の測定法についての検討(農学部門)

実験1∿5において4鶏種より得た1040個の鶏卵について, 材料検査機と富士平式卵殻強度計で長径方向又は短径方向より加圧して, 卵殻破壊強度を測定し, その卵殻の厚さと鶏卵比重との相関係数を求め, 卵殻破壊強度の測定方法について2・3の検討を行なった。卵殻破壊強度は材料検査機の方が高い値を示し, 加圧方向より見れば, 短径方向より加圧した方が低い値を示し, 変動係数も低かった。また, 両機の卵殻破壊強度と卵殻の厚さの間にr=(0.42)^∿(0.84)^, 卵殻破壊強度と鶏卵比重との間にr=0.44∿^(0.74)^の有意な相関が認められた。長径方向より加圧した場合, 富士平式卵殻強度計値X_1と材料検査機の卵殻破壊値Y_1 (kg)の間にY_1 (kg)=0.27+1.12X_1 (r=(0.93)^)また, 短径方向より加圧した場合, 富士平式卵殻強度計X^2と材料検査機の卵殻破壊強度値Y_2(kg)の間にY_2(kg)=-0.22+1.25X_2(r=(0.97)^)が得られた。The present study was conducted to compare the performances of two apparatuses and the derection of application in measuring egg shell strength. A total of 1040 eggs, collected from four commercial laying strains which were fed in wire cages, were used in five frials. Two readings were obtained using two apparatuses in the following : A) a testing machine (Tensilon UTM-4L type) and Fujihira egg shell strength tester. These two readings were made with of the compressive force measuring along the major axis and the minor axis of the egg. Specific gravity of the eggs was deterimined by immersing them in a series of saline solutions of ranging from 1.060 to 1.115. The average shell thickness of three points at the equatorial region was measured to the nearest 10μ.m. using a vice caliper. The shell membrances were removed in this measurement. All date were subjected to correlation analyses and regression analyses. The results are summarized as follows : 1) The egg shell strength measured by the Fujihira shell strength tester was lower than that by the Tensilon UTM-4L. 2) The egg shell strength and its coefficients of variation measured along the minor axis were lower than those along the major axis. 3) The correlation coefficients between the shell strength measured by both apparatuses and the shell thickness, ranged from (0.42)^ to (0.85)^ in all trial, and all values were highly significant. 4) The correlation coefficients between shell strength and egg specific gravity, ranged from (0.44)^ to (0.74)^ with all values being highly significant. These correlation coefficients were in good apreement with the date by many other researchers. 5) Linear relationship was found between the egg Strength (X)=measured by Fujihira shell strength tester and the egg strength (Y)=measured by Tensilon UTM-4L. 6) Regression formulas between those two are as follows : i) Compression along the major axis : Y_1 (kg)=0.27+1.12X_1 (r=(0.93)^) where Y_1(kg)=shell strength by the Tensilon UTM-4L, X_1=shell strength by the Fujihira shell strength tester. ii) Compression along the minor axis : Y_2(kg)=-0.22+1.25X_2(r=(0.97)^) where Y_2(kg)=shell strength by the Tensiron UTM-4L, X_2=shell strength by the Fujihira shell strengh tester

Research and Design of a Routing Protocol in Large-Scale Wireless Sensor Networks

无线传感器网络，作为全球未来十大技术之一，集成了传感器技术、嵌入式计算技术、分布式信息处理和自组织网技术，可实时感知、采集、处理、传输网络分布区域内的各种信息数据，在军事国防、生物医疗、环境监测、抢险救灾、防恐反恐、危险区域远程控制等领域具有十分广阔的应用前景。 本文研究分析了无线传感器网络的已有路由协议，并针对大规模的无线传感器网络设计了一种树状路由协议，它根据节点地址信息来形成路由，从而简化了复杂繁冗的路由表查找和维护，节省了不必要的开销，提高了路由效率，实现了快速有效的数据传输。 为支持此路由协议本文提出了一种自适应动态地址分配算——ADAR（AdaptiveDynamicAddre...As one of the ten high technologies in the future, wireless sensor network, which is the integration of micro-sensors, embedded computing, modern network and Ad Hoc technologies, can apperceive, collect, process and transmit various information data within the region. It can be used in military defense, biomedical, environmental monitoring, disaster relief, counter-terrorism, remote control of haz...学位：工学硕士院系专业：信息科学与技术学院通信工程系_通信与信息系统学号：2332007115216