132 research outputs found
Generation of Ultrastable Microwaves via Optical Frequency Division
There has been increased interest in the use and manipulation of optical
fields to address challenging problems that have traditionally been approached
with microwave electronics. Some examples that benefit from the low
transmission loss, agile modulation and large bandwidths accessible with
coherent optical systems include signal distribution, arbitrary waveform
generation, and novel imaging. We extend these advantages to demonstrate a
microwave generator based on a high-Q optical resonator and a frequency comb
functioning as an optical-to-microwave divider. This provides a 10 GHz
electrical signal with fractional frequency instability <8e-16 at 1 s, a value
comparable to that produced by the best microwave oscillators, but without the
need for cryogenic temperatures. Such a low-noise source can benefit radar
systems, improve the bandwidth and resolution of communications and digital
sampling systems, and be valuable for large baseline interferometry, precision
spectroscopy and the realization of atomic time
Frequency-stabilization to 6x10^-16 via spectral-hole burning
We demonstrate two-stage laser stabilization based on a combination of Fabry-
Perot and spectral-hole burning techniques. The laser is first pre-stabilized
by the Fabry-Perot cavity to a fractional-frequency stability of sigma_y(tau) <
10^-13. A pattern of spectral holes written in the absorption spectrum of
Eu3+:Y2SiO5 serves to further stabilize the laser to sigma_y(tau) = 6x10^-16
for 2 s < tau < 8 s. Measurements characterizing the frequency sensitivity of
Eu3+:Y2SiO5 spectral holes to environmental perturbations suggest that they can
be more frequency stable than Fabry-Perot cavities
Dual-pump Kerr micro-cavity optical frequency comb with varying FSR spacing
In this paper, we demonstrate a novel dual-pump approach to generate robust optical frequency comb with varying free spectral range (FSR) spacing in a CMOS-compatible high-Q micro-ring resonator (MRR). The frequency spacing of the comb can be tuned by an integer number FSR of the MRR freely in our dual-pump scheme. The dual pumps are self-oscillated in the laser cavity loop and their wavelengths can be tuned flexibly by programming the tunable filter embedded in the cavity. By tuning the pump wavelength, broadband OFC with the bandwidth of >180nm and the frequency-spacing varying from 6 to 46-fold FSRs is realized at a low pump power. This approach could find potential and practical applications in many areas, such as optical metrology, optical communication, and signal processing systems, for its excellent flexibility and robustness
Higher occurrence of nausea and vomiting after total hip arthroplasty using general versus spinal anesthesia: an observational study.
BACKGROUND: Under the assumption that postoperative nausea and vomiting (PONV) may occur after total hip arthroplasty (THA) regardless of the anesthetic technique used, it is not clear whether general (GA) or spinal (SA) anesthesia has higher causal effect on this occurrence. Conflicting results have been reported.
METHODS: In this observational study, we selected all elective THA interventions performed in adults between 1999 and 2008 in a Swiss orthopedic clinic under general or spinal anesthesia. To assess the effect of anesthesia type on the occurrence of PONV, we used the propensity score and matching methods, which allowed us to emulate the design and results of an RCT.
RESULTS: Among 3922 procedures, 1984 (51Â %) patients underwent GA, of which 4.1Â % experienced PONV, and 1938 underwent SA, of which 3.5Â % experienced PONV. We found that the average treatment effect on the treated, i.e. the effect of anesthesia type for a sample of individuals that actually received spinal anesthesia compared to individuals who received GA, was ATETâ=â2.00Â % [95Â % CI, 0.78-3.19Â %], which translated into an ORâ=â1.97 [95Â % CI 1.35; 2.87].
CONCLUSION: This suggests that the type of anesthesia is not neutral regarding PONV, general anesthesia being more strongly associated with PONV than spinal anesthesia in orthopedic surgery
Comb-Based Radio-Frequency Photonic Filters with Rapid Tunability and High Selectivity
Photonic technologies have received considerable attention for enhancement of
radio-frequency (RF) electrical systems, including high-frequency analog signal
transmission, control of phased arrays, analog-to-digital conversion, and
signal processing. Although the potential of radio-frequency photonics for
implementation of tunable electrical filters over broad RF bandwidths has been
much discussed, realization of programmable filters with highly selective
filter lineshapes and rapid reconfigurability has faced significant challenges.
A new approach for RF photonic filters based on frequency combs offers a
potential route to simultaneous high stopband attenuation, fast tunability, and
bandwidth reconfiguration. In one configuration tuning of the RF passband
frequency is demonstrated with unprecedented (~40 ns) speed by controlling the
optical delay between combs. In a second, fixed filter configuration, cascaded
four-wave mixing simultaneously broadens and smoothes comb spectra, resulting
in Gaussian RF filter lineshapes exhibiting extremely high (>60 dB) main lobe
to sidelobe suppression ratio and (>70 dB) stopband attenuation.Comment: Updated the submission with the most recent version of the pape
Uranyl oxo activation and functionalization by metal cation coordination
International audienceThe oxo groups in the uranyl ion [UO] , one of many oxo cations formed by metals from across the periodic tableâare particularly inert, which explains the dominance of this ion in the laboratory and its persistence as an environmental contaminant. In contrast, transition metal oxo (M=O) compounds can be highly reactive and carry out difficult reactions such as the oxygenation of hydrocarbons. Here we show how the sequential addition of a lithium metal base to the uranyl ion constrained in a âPacmanâ environment results in lithium coordination to the U=O bonds and single-electron reduction. This reaction depends on the nature and stoichiometry of the lithium reagent and suggests that competing reduction and CâH bond activation reactions are occurring
Study of Leishmania pathogenesis in mice : experimental considerations
Although leishmaniases are endemic in 98 countries, they are still considered neglected tropical diseases. Leishmaniases are characterized by the emergence of new virulent and asymptomatic strains of Leishmania spp. and, as a consequence, by a very diverse clinical spectrum. To fight more efficiently these parasites, the mechanisms of host defense and of parasite virulence need to be thoroughly investigated. To this aim, animal models are widely used. However, the results obtained with these models are influenced by several experimental parameters, such as the mouse genetic background, parasite genotype, inoculation route/infection site, parasite dose and phlebotome saliva. In this review, we propose an update on their influence in the two main clinical forms of the disease: cutaneous and visceral leishmaniases
Isolation and Mutagenesis of a Capsule-Like Complex (CLC) from Francisella tularensis, and Contribution of the CLC to F. tularensis Virulence in Mice
BACKGROUND: Francisella tularensis is a category-A select agent and is responsible for tularemia in humans and animals. The surface components of F. tularensis that contribute to virulence are not well characterized. An electron-dense capsule has been postulated to be present around F. tularensis based primarily on electron microscopy, but this specific antigen has not been isolated or characterized. METHODS AND FINDINGS: A capsule-like complex (CLC) was effectively extracted from the cell surface of an F. tularensis live vaccine strain (LVS) lacking O-antigen with 0.5% phenol after 10 passages in defined medium broth and growth on defined medium agar for 5 days at 32°C in 7% COâ. The large molecular size CLC was extracted by enzyme digestion, ethanol precipitation, and ultracentrifugation, and consisted of glucose, galactose, mannose, and Proteinase K-resistant protein. Quantitative reverse transcriptase PCR showed that expression of genes in a putative polysaccharide locus in the LVS genome (FTL_1432 through FTL_1421) was upregulated when CLC expression was enhanced. Open reading frames FTL_1423 and FLT_1422, which have homology to genes encoding for glycosyl transferases, were deleted by allelic exchange, and the resulting mutant after passage in broth (LVSÎ1423/1422_P10) lacked most or all of the CLC, as determined by electron microscopy, and CLC isolation and analysis. Complementation of LVSÎ1423/1422 and subsequent passage in broth restored CLC expression. LVSÎ1423/1422_P10 was attenuated in BALB/c mice inoculated intranasally (IN) and intraperitoneally with greater than 80 times and 270 times the LVS LDâ
â, respectively. Following immunization, mice challenged IN with over 700 times the LDâ
â of LVS remained healthy and asymptomatic. CONCLUSIONS: Our results indicated that the CLC may be a glycoprotein, FTL_1422 and -FTL_1423 were involved in CLC biosynthesis, the CLC contributed to the virulence of F. tularensis LVS, and a CLC-deficient mutant of LVS can protect mice against challenge with the parent strain
School Effects on the Wellbeing of Children and Adolescents
Well-being is a multidimensional construct, with psychological, physical and social components. As theoretical basis to help understand this concept and how it relates to school, we propose the Self-Determination Theory, which contends that self-determined motivation and personality integration, growth and well-being are dependent on a healthy balance of three innate psychological needs of autonomy, relatedness and competence. Thus, current indicators involve school effects on childrenâs well-being, in many diverse modalities which have been explored. Some are described in this chapter, mainly: the importance of peer relationships; the benefits of friendship; the effects of schools in conjunction with some forms of family influence; the school climate in terms of safety and physical ecology; the relevance of the teacher input; the school goal structure and the implementation of cooperative learning. All these parameters have an influence in promoting optimal functioning among children and increasing their well-being by meeting the above mentioned needs. The empirical support for the importance of schools indicates significant small effects, which often translate into important real-life effects as it is admitted at present. The conclusion is that schools do make a difference in childrenâs peer relationships and well-being
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