4,555 research outputs found
Reinforced structural plastics
Reinforced polyimide structures are described. Reinforcing materials are impregnated with a suspension of polyimide prepolymer and bonded together by heat and pressure to form a cured, hard-reinforced, polyimide structure
Algorithms for 3D rigidity analysis and a first order percolation transition
A fast computer algorithm, the pebble game, has been used successfully to
study rigidity percolation on 2D elastic networks, as well as on a special
class of 3D networks, the bond-bending networks. Application of the pebble game
approach to general 3D networks has been hindered by the fact that the
underlying mathematical theory is, strictly speaking, invalid in this case. We
construct an approximate pebble game algorithm for general 3D networks, as well
as a slower but exact algorithm, the relaxation algorithm, that we use for
testing the new pebble game. Based on the results of these tests and additional
considerations, we argue that in the particular case of randomly diluted
central-force networks on BCC and FCC lattices, the pebble game is essentially
exact. Using the pebble game, we observe an extremely sharp jump in the largest
rigid cluster size in bond-diluted central-force networks in 3D, with the
percolating cluster appearing and taking up most of the network after a single
bond addition. This strongly suggests a first order rigidity percolation
transition, which is in contrast to the second order transitions found
previously for the 2D central-force and 3D bond-bending networks. While a first
order rigidity transition has been observed for Bethe lattices and networks
with ``chemical order'', this is the first time it has been seen for a regular
randomly diluted network. In the case of site dilution, the transition is also
first order for BCC, but results for FCC suggest a second order transition.
Even in bond-diluted lattices, while the transition appears massively first
order in the order parameter (the percolating cluster size), it is continuous
in the elastic moduli. This, and the apparent non-universality, make this phase
transition highly unusual.Comment: 28 pages, 19 figure
New rapid-curing, stable polyimide polymers with high-temperature strength and thermal stability
Additive-type polymerization reaction forms thermally stable polyimide polymers, thereby eliminating the volatile matter attendant with the condensation reaction. It is based on the utilization of reactive alicyclic rings positioned on the ends of polyimide prepolymers having relatively low molecular weights
Terminal velocity and drag reduction measurements on superhydrophobic spheres
Super water-repellent surfaces occur naturally on plants and aquatic insects and are created in the laboratory by combining micro- or nanoscale surface topographic features with hydrophobic surface chemistry. When such types of water-repellent surfaces are submerged they can retain a film of air (a plastron). In this work, we report measurements of the terminal velocity of solid acrylic spheres with various surface treatments settling under the action of gravity in water. We observed increases in terminal velocity corresponding to drag reduction of between 5% and 15% for superhydrophobic surfaces that carry plastrons
Science aspects of a 1980 flyby of Comet Encke with a Pioneer spacecraft
Results are presented of an investigation of the feasibility of a 1980 flyby of Comet Encke using a Pioneer class spacecraft. Specific areas studied include: science objectives and rationale; science observables; effects of encounter velocity; science encounter and targeting requirements; selection and description of science instruments; definition of a candidate science payload; engineering characteristics of suggested payload; value of a separable probe; science instruments for a separable probe; science payload integration problems; and science operations profile
Science aspects of 1980 ballistic missions to comet Encke, using Mariner and Pioneer spacecraft
Science aspects of a 1980 spacecraft reconnaissance of Comet Encke are considered. The mission discussed is a ballistic flyby (more exactly, a fly-through) of P/Encke, using either a spin stabilized spacecraft, without despin of instruments, or a 3-axis stabilized spacecraft
Rigidity analysis of HIV-1 protease
We present a rigidity analysis on a large number of X-ray crystal structures
of the enzyme HIV-1 protease using the 'pebble game' algorithm of the software
FIRST. We find that although the rigidity profile remains similar across a
comprehensive set of high resolution structures, the profile changes
significantly in the presence of an inhibitor. Our study shows that the action
of the inhibitors is to restrict the flexibility of the beta-hairpin flaps
which allow access to the active site. The results are discussed in the context
of full molecular dynamics simulations as well as data from NMR experiments.Comment: 4 pages, 3 figures. Conference proceedings for CMMP conference 2010
which was held at the University of Warwic
Glycation of Amino Groups in Protein
Ribonuclease A has been used as a model protein for studying the specificity of glycation of amino groups in protein under physiological conditions (phosphate buffer, pH 7.4, 37 “C). Incubation of RNase with glucose led to an enhanced rate of inactivation of the enzyme relative to the rate of modification of lysine residues, suggesting preferential modification of active site lysine residues. Sites of glycation of RNase were identified by amino acid analysis of tryptic peptides isolated by reverse-phase high pressure liquid chromatography and phenylboronate affinity chromatography. Schiff base adducts were trapped with Na- BH&N and the a-amino group of Lys-1 was identified as the primary site (80-90%) of initial Schiff base formation on RNase. In contrast, Lys-41 and Lys-7 in the active sitaec counted for about 38 and 29%, respectively, of ketoamine adducts formed via the Amadori rearrangement. Other sites reactive in ketoamine formation included Ne-Lys-1 (15%), N-Lys-1 (9%), and Lys-37 (9%w) hich are adjacent to acidic amino acids. The remaining six lysine residues in RNase, which are located on the surface of the protein, were relatively inactive in forming either the Schiff base or Amadori adduct. Both the equilibrium Schiff base concentration and the rate of the Amadori rearrangement at each site were found to be important in determining the specificity of glycation of RNase
Inulin-\u3csup\u3e125\u3c/sup\u3eI-Tyramine, an Improved Residualizing Label for Studies on Sites of Catabolism of Circulating Proteins
Residualizing labels for protein, such as dilactitol-125I-tyramine (125I-DLT) and cellobiitol-125I-tyramine, have been used to identify the tissue and cellular sites of catabolism of long-lived plasma proteins, such as albumin, immunoglobulins, and lipoproteins. The radioactive degradation products formed from labeled proteins are relatively large, hydrophilic, resistant to lysosomal hydrolases, and accumulate in lysosomes in the cells involved in degradation of the carrier protein. However, the gradual loss of the catabolites from cells (t1/2 approximately 2 days) has limited the usefulness of residualizing labels in studies on longer lived proteins. We describe here a higher molecular weight (Mr approximately 5000), more efficient residualizing glycoconjugate label, inulin-125I-tyramine (125I-InTn). Attachment of 125I-InTn had no effect on the plasma half-life or tissue sites of catabolism of asialofetuin, fetuin, or rat serum albumin in the rat. The half-life for hepatic retention of degradation products from 125I-InTn-labeled asialofetuin was 5 days, compared to 2.3 days for 125I-DLT-labeled asialofetuin. The whole body half-lives for radioactivity from 125I-InTn-, 125I-DLT-, and 125I-labeled rat serum albumin were 7.5, 4.3, and 2.2 days, respectively. The tissue distribution of degradation products from 125I-InTn-labeled proteins agreed with results of previous studies using 125I-DLT, except that a greater fraction of total degradation products was recovered in tissues. Kinetic analyses indicated that the average half-life for retention of 125I-InTn degradation products in tissues is approximately 5 days and suggested that in vivo there are both slow and rapid routes for release of degradation products from cells. Overall, these experiments indicate that 125I-InTn should provide greater sensitivity and more accurate quantitative information on the sites of catabolism of long-lived circulating proteins in vivo
Nonenzymatically Glucosylated Albumin: In Vitro Preparation and Isolation from Normal Human Serum
Incubation of human serum with D-[6-3H]glucose resulted in the gradual accumulation of radioactivity in acid-precipitable material. Upon chromatography on Sephadex G-200, radioactivity was found associated with each of the major molecular weight classes of serum protein. Purified human serum albumin was also glucosylated in vitro upon exposure to D-[6-3H]glucose in phosphate-buffered saline. The glucosylated and unmodified albumins were separated by ion exchange chromatography. The physiological significance of these observations in vitro was confirmed by the isolation and quantitation of glucosylated albumin from normal human serum. Glucosylated albumin represents approximately 6 to 15% of total serum albumin in normal adults. The post-translational modification appears to occur by a nonenzymatic process analogous to that responsible for glucosylation of hemoglobin A to hemoglobin AIc, i.e. through Schiff base formation and Amadori rearrangement to a ketoamine derivative
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