Caracterizacao morfologica de germoplasma de batata-doce mantido pela EPAGRI.

bitstream/item/107309/1/Caracterizacao-morfologica-do-germoplasma-de-batata-doce.pd

Avian influenza virus (H11N9) in migratory shorebirds wintering in the Amazon region, Brazil

Aquatic birds are the natural reservoir for avian influenza viruses (AIV). Habitats in Brazil provide stopover and wintering sites for water birds that migrate between North and South America. The current study was conducted to elucidate the possibility of the transport of influenza A viruses by birds that migrate annually between the Northern and Southern Hemispheres. In total, 556 orotracheal/cloacal swab samples were collected for influenza A virus screening using real-time RT-PCR (rRT-PCR). The influenza A virus-positive samples were subjected to viral isolation. Four samples were positive for the influenza A matrix gene by rRT-PCR. From these samples, three viruses were isolated, sequenced and characterized. All positive samples originated from a single bird species, the ruddy turnstone (Arenaria interpres), that was caught in the Amazon region at Caeté Bay, Northeast Pará, at Ilha de Canelas. To our knowledge, this is the first isolation of H11N9 in the ruddy turnstone in South America. (Résumé d'auteur

Produção de sementes de cebola.

bitstream/item/199077/1/Producao-de-Sementes-de-Cebola.pd

Respiratory syncytial virus epidemic periods in an equatorial city of Brazil

Submitted by Sandra Infurna ([email protected]) on 2017-01-24T14:14:17Z No. of bitstreams: 1 marilda_siqueira_etal_IOC_2013.pdf: 274921 bytes, checksum: baf4e66faf10e03f01d0baa4bacf33f4 (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2017-01-24T14:23:30Z (GMT) No. of bitstreams: 1 marilda_siqueira_etal_IOC_2013.pdf: 274921 bytes, checksum: baf4e66faf10e03f01d0baa4bacf33f4 (MD5)Made available in DSpace on 2017-01-24T14:23:30Z (GMT). No. of bitstreams: 1 marilda_siqueira_etal_IOC_2013.pdf: 274921 bytes, checksum: baf4e66faf10e03f01d0baa4bacf33f4 (MD5) Previous issue date: 2013Universidade Federal do Ceará. Fortaleza, CE, Brasil.Universidade Federal do Ceará. Fortaleza, CE, Brasil.Universidade Federal do Ceará. Fortaleza, CE, Brasil.Universidade Federal do Ceará. Fortaleza, CE, Brasil.Universidade Federal do Ceará. Fortaleza, CE, Brasil.Universidade Federal do Ceará. Fortaleza, CE, Brasil.Instituto Butantan. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.Universidade de São Paulo. São Paulo, SP, Brasil.Universidade de São Paulo. São Paulo, SP, Brasil.Universidade de São Paulo. São Paulo, SP, Brasil.Universidade de São Paulo. São Paulo, SP, Brasil.Characterization of the human respiratory syncytial virus (HRSV) season at the local level has important implications for appropriate decisions on the time period for administration of specific prophylaxis

Persistence and Intra-Host Genetic Evolution of Zika Virus Infection in Symptomatic Adults: A Special View in the Male Reproductive System

We followed the presence of Zika virus (ZIKV) in four healthy adults (two men and two women), for periods ranging from 78 to 298 days post symptom onset. The patients were evaluated regarding the presence of the virus in different body fluids (blood, saliva, urine and semen), development of immune responses (including antibodies, cytokines and chemokines), and virus genetic variation within samples collected from semen and urine during the infection course. The analysis was focused primarily on the two male patients who shed the virus for up to 158 days after the initial symptoms. ZIKV particles were detected in the spermatozoa cytoplasm and flagella, in immature sperm cells and could also be isolated from semen in cell culture, confirming that the virus is able to preserve integrity and infectivity during replication in the male reproductive system (MRS). Despite the damage caused by ZIKV infection within the MRS, our data showed that ZIKV infection did not result in infertility at least in one of the male patients. This patient was able to conceive a child after the infection. We also detected alterations in the male genital cytokine milieu, which could play an important role in the replication and transmission of the virus which could considerably increase the risk of ZIKV sexual spread. In addition, full genome ZIKV sequences were obtained from several samples (mainly semen), which allowed us to monitor the evolution of the virus within a patient during the infection course. We observed genetic changes over time in consensus sequences and lower frequency intra-host single nucleotide variants (iSNV), that suggested independent compartmentalization of ZIKV populations in the reproductive and urinary systems. Altogether, the present observations confirm the risks associated with the long-term replication and shedding of ZIKV in the MRS and help to elucidate patterns of intra-host genetic evolution during long term replication of the virus

Phylogenetic analysis of neuraminidase of the influenza A virus.

<p>Trees were generated by PAUP 4.0b10 using the maximum likelihood algorithm with the parameters indicated by the GTR Modeltest (bootstrap values are shown for the branch points). The scale bar is shown on the bottom left. In this study, sequences that were available in public databases were used to construct the tree, and the accession numbers along with their branch data are shown.</p

Phylogenetic analysis of the hemagglutinin gene of the influenza A virus.

<p>Trees were generated by PAUP 4.0b10 using the maximum likelihood algorithm with the parameters indicated by the GTR Modeltest (bootstrap values are shown for the branch points). The scale bar is shown on the bottom left. In this study, sequences that were available in the public database were used to construct the tree, and the accession numbers along with their branch data are shown.</p