Mycobacterium tuberculosis ecology in Venezuela: epidemiologic correlates of common spoligotypes and a large clonal cluster defined by MIRU-VNTR-24

<p>Abstract</p> <p>Background</p> <p>Tuberculosis remains an endemic public health problem, but the ecology of the TB strains prevalent, and their transmission, can vary by country and by region. We sought to investigate the prevalence of <it>Mycobacterium tuberculosis </it>strains in different regions of Venezuela. A previous study identified the most prevalent strains in Venezuela but did not show geographical distribution nor identify clonal genotypes. To better understand local strain ecology, we used spoligotyping to analyze 1298 <it>M. tuberculosis </it>strains isolated in Venezuela from 1997 to 2006, predominantly from two large urban centers and two geographically distinct indigenous areas, and then studied a subgroup with MIRU-VNTR 24 loci.</p> <p>Results</p> <p>The distribution of spoligotype families is similar to that previously reported for Venezuela and other South American countries: LAM 53%, T 10%, Haarlem 5%, S 1.9%, X 1.2%, Beijing 0.4%, and EAI 0.2%. The six most common shared types (SIT's 17, 93, 605, 42, 53, 20) accounted for 49% of the isolates and were the most common in almost all regions, but only a minority were clustered by MIRU-VNTR 24. One exception was the third most frequent overall, SIT 605, which is the most common spoligotype in the state of Carabobo but infrequent in other regions. MIRU-VNTR homogeneity suggests it is a clonal group of strains and was named the "Carabobo" genotype. Epidemiologic comparisons showed that patients with SIT 17 were younger and more likely to have had specimens positive for Acid Fast Bacilli on microscopy, and patients with SIT 53 were older and more commonly smear negative. Female TB patients tended to be younger than male patients. Patients from the high incidence, indigenous population in Delta Amacuro state were younger and had a nearly equal male:female distribution.</p> <p>Conclusion</p> <p>Six SIT's cause nearly half of the cases of tuberculosis in Venezuela and dominate in nearly all regions. Strains with SIT 17, the most common pattern overall may be more actively transmitted and SIT 53 strains may be less virulent and associated with reactivation of past infections in older patients. In contrast to other common spoligotypes, strains with SIT 605 form a clonal group centered in the state of Carabobo.</p

Modeling the effects of ICT on environmental sustainability: revisiting a system dynamics model developed for the european commission

This chapter revisits a System Dynamics model developed in 2002 with the aim of exploring the future impacts of Information and Communication Technology (ICT) on environmental sustainability in the EU, which then consisted of 15 countries. The time horizon of the study was 20 years (2000-2020). We analyze the results in light of empirical data that is now available for 2000-2012. None of the three scenarios that were developed by experts to specify the external factors needed to run the model were realistic from today’s point of view. If the model is re-run with more realistic input data for the first half of the simulation period, however, the main results regarding the impact of ICT remain qualitatively the same; they seem to be relatively robust implications of the causal system structure, as it is represented in the model. Overall, the impacts of ICT for mitigating greenhouse gas emissions and other environmental burdens for 2020 tend to be slightly stronger if the simulation is based on the empirical data now available

MoS2 monolayer catalyst doped with isolated Co atoms for the hydrodeoxygenation reaction

The conversion of oxygen-rich biomass into hydrocarbon fuels requires efficient hydrodeoxygenation catalysts during the upgrading process. However, traditionally prepared CoMoS2 catalysts, although efficient for hydrodesulfurization, are not appropriate due to their poor activity, sulfur loss and rapid deactivation at elevated temperature. Here, we report the synthesis of MoS2 monolayer sheets decorated with isolated Co atoms that bond covalently to sulfur vacancies on the basal planes that, when compared with conventionally prepared samples, exhibit superior activity, selectivity and stability for the hydrodeoxygenation of 4-methylphenol to toluene. This higher activity allows the reaction temperature to be reduced from the typically used 300 °C to 180 °C and thus allows the catalysis to proceed without sulfur loss and deactivation. Experimental analysis and density functional theory calculations reveal a large number of sites at the interface between the Co and Mo atoms on the MoS2 basal surface and we ascribe the higher activity to the presence of sulfur vacancies that are created local to the observed Co–S–Mo interfacial sites

Optimising Hamstring Strength and Function for Performance After Hamstring Injury

Hamstring strain injury often results in neuromuscular performance deficits that persist beyond rehabilitation and the return to full training and competitive sport. It seems appropriate to address these deficits as a part of a sport-specific training program which primarily aims to enhance performance. Prolonged deficits in horizontal ground reaction forces in sprinting, repeat sprint performance, knee flexor eccentric strength and biceps femoris long head fascicle lengths have been observed in multiple studies of hamstring strain injury. Why such deficits persist beyond the return to sport is not known, although persistent neuromuscular inhibition of the injured muscles has been proposed. There is limited and mixed evidence for sprint running kinematic (technique) differences between previously injured and uninjured limbs or athletes, although more work in this area seems warranted. While there is some uncertainty about the optimal mix of methods for addressing the aforementioned deficits, sport-specific running programs in conjunction with continued monitoring of acceleration phase sprint performance and repeated sprint ability seem appropriate. Heavy strength training with at least some eccentrically biased exercises is also recommended to address deficits in eccentric strength and muscle fascicle lengths