FREE MOVEMENT OF WORKERS: MIGRATION IN THE EUROPEAN UNION

ABSTRACT Objective: This article is intended for studies and research on the free movement of workers within the European Union, the purpose of which is to provide a critical analysis of not only its current foundations, but also to provide contributions to demonstrate that the migratory flow of workers is fundamental for maintaining the integration of the respective economic bloc. Methodology:The methodology used in the studies and research carried out is the critical-reflexive one, which operates through a bibliographic review and the analysis of concrete cases that are jurisdictionalized before the Court of Justice of the European Union. Results: The existence of a legal framework is of paramount importance for maintaining the free movement of workers within the European Union, so that their workers can settle in another State and exercise their craft in a dignified manner, with the right covered by labor and social security legislation, under penalty of violation of the provisions of the Charter of Fundamental Rights of Europe. Contributions: The demonstration of the importance of worker migration within the European Community, with respect to the guarantee of fundamental freedoms and non-discrimination. Such a guarantee is not understandable, finally, without a reflection on the contemporary role of the Court of Justice of the European Union and its extended jurisdiction. Keywords: European Union; free movement; workers; migration. RESUMO Objetivo: O presente artigo destina-se aos estudos e pesquisas sobre a livre circulação de trabalhadores na União Europeia, cujo objetivo é o de oferecer uma análise crítica acerca não só dos seus atuais fundamentos, mas, para também assim oferecer contribuições que se destinam a demonstrar que o fluxo migratório de trabalhadores é fundamental para a manutenção da integração do respectivo bloco econômico. Metodologia: A metodologia empregada nos estudos e nas pesquisas até então levados a cabo é crítico-reflexiva, que se opera através da revisão bibliográfica e da análise de casos concretos que se encontram jurisdicionalizados perante o Tribunal de Justiça da União Europeia. Resultados: A existência de um marco jurídico legal revela-se de suma importância para a manutenção da livre circulação de trabalhadores na União Europeia, a fim de que os respectivos trabalhadores possam estabelecer-se em outro Estado e exercerem o seu ofício de forma digna, com direito à residência e abarcados, inclusive, pela legislação trabalhista e previdenciária, sob pena de violação aos preceitos da Carta dos Direitos Fundamentais da Europa. Contribuições: A demonstração da importância da migração de trabalhadores na Comunidade Europeia, em respeito à garantia das liberdades fundamentais e a não discriminação. Tal garantia não é compreensível, por fim, sem uma reflexão quanto ao papel contemporâneo do Tribunal de Justiça da União Europeia e sua jurisdição estendida.Palavras-chave: União Europeia; liberdade de circulação; trabalhadores; migração.

Combining advantages from parameters in modeling and control of discrete event systems

Although Finite-State Automata (FSA) have been successfully used in modeling and control of Discrete Event Systems (DESs), they are limited to represent complex and advanced features of DESs, such as context recognition and switching. The literature has suggested that a FSA can nevertheless be enriched with parameters properly collected from the modeled system, so that this favors design and control. A parameter can be embedded either on transitions or states. However, each approach is structured within a specific framework, so that their comparison and integration are not straightforward and they may lead to different control solutions, modeled, computed and implemented using distinct strategies. In this paper, we show how to combine advantages from parameters in modeling and control of DESs. Each approach is structured and their advantages are identified and exemplified. Then, we propose a conversion method that allows to translate a design-friendly model into a synthesis-efficient structure. Examples illustrate the approach.CNPq, under grant number 402145/2016-0, 09, Araucaria Foundation, CAPES, and FINEP, and partially supported by ERDF - The European Regional Development Fund through the Operational Programme for Competitiveness and Internationalisation - COMPETE 2020 Programme, and by National Funds through FCT - Fundação para a Ciência e a Tecnologia, within project POCI- ˆ 01-0145-FEDER-030947 (KLEE

NEOCONSTITUCIONALISMO: AS CONSEQUÊNCIAS DA ATUAÇÃO DO PODER JUDICIÁRIO PARA A DEMOCRACIA BRASILEIRA

RESUMO Esse resumo se refere aos estudos e pesquisas sobre a atuação do Poder Judiciário brasileiro e as consequências do ativismo judicial para a democracia brasileira, trazendo ao debate as questões do novo constitucionalismo e o papel do juiz na contemporaneidade. O escopo deste artigo analisa, previamente, as diferenças e semelhanças havidas entre os sistemas da commom law e da civil law e sua aparente progressiva convergência

Production and characterization of protease from Penicillium aurantiogriseum URM 4622

Proteases with new properties are required due to their increasing industrial importance. In this work, the optimal fermentation conditions for the production of a protease from Penicillium aurantiogriseum dierchx (URM-4622) are presented together with partial characterization of the protease catalytic properties. The batch fermentation conditions that allow for the highest specific proteolytic activity are 26 ºC, pH 7.0, and 25 % saturation dissolved O2 concentration. The obtained protease is stable over a wide range of pH (5.8 to 9.5) and temperature (25 to 40 ºC) values. In the presence of Zn2+ a 26 % reduction in the enzyme proteolytic activity occurs and, in contrast, Mn2+ enhances its activity by 28.9 %. 96.2 % and 70.8 % of the protease activity are maintained after 90 min incubation in 5 and 10 % (v/v) H2O2 aqueous solutions, respectively. PMSF inhibition reveals that this enzyme is a serine protease. Protease is able to hydrolyze different proteins

Putative biomarkers for cervical cancer: SNVs, methylation and expression profiles

Cervical cancer is primarily caused by Human papillomavirus (HPV) infection, but other factors such as smoking habits, co-infections and genetic background, can also contribute to its development. Although this cancer is avoidable, it is the fourth most frequent type of cancer in females worldwide and can only be treated with chemotherapy and radical surgery. There is a need for biomarkers that will enable early diagnosis and targeted therapy for this type of cancer. Therefore, a systems biology pipeline was applied in order to identify potential biomarkers for cervical cancer, which show significant reports in three molecular aspects: DNA sequence variants, DNA methylation pattern and alterations in mRNA/protein expression levels. CDH1, CDKN2A, RB1 and TP53 genes were selected as putative biomarkers, being involved in metastasis, cell cycle regulation and tumour suppression. The other ten genes (CDH13, FHIT, PTEN, MLH1, TP73, CDKN1A, CACNA2D2, TERT, WIF1, APC) seemed to play a role in cervical cancer, but the lack of studies prevented their inclusion as possible biomarkers. Our results highlight the importance of these genes. However, further studies should be performed to elucidate the impact of DNA sequence variants and/or epigenetic deregulation and altered expression of these genes in cervical carcinogenesis and their potential as biomarkers for cervical cancer diagnosis and prognosis.This work was funded by the Foundation for Science and Technology of the state of Pernambuco (FACEPE). The authors are grateful to Vinicius Albertin Tigre da Costa for his contributions to the construction of the figures.info:eu-repo/semantics/publishedVersio

Probiotic and antifungal attributes of lactic acid bacteria isolates from naturally fermented Brazilian table olives

Research with fermented olives as a source of wild Lactic Acid Bacteria (LAB) strains with probiotic and biotechnological characteristics constitutes a promising field of work. The present study evaluated in vitro probiotic, antifungal, and antimycotoxigenic potential of LAB isolates from naturally fermented Brazilian table olives. Among fourteen LAB isolates, the Levilactobacillus brevis CCMA 1762, Lactiplantibacillus pentosus CCMA 1768, and Lacticaseibacillus paracasei subsp. paracasei CCMA 1770 showed potential probiotic and antifungal properties. The isolates showed resistance to pH 2.0 (survival ≥ 84.55), bile salts (survival ≥ 99.44), and gastrointestinal tract conditions (survival ≥ 57.84%); hydrophobic cell surface (≥27%); auto-aggregation (≥81.38%); coaggregation with Escherichia coli INCQS 00181 (≥33.97%) and Salmonella Enteritidis ATCC 564 (≥53.84%); adhesion to the epithelial cell line Caco-2 (≥5.04%); antimicrobial activity against the bacteria S. Enteritidis ATCC 564 (≥6 mm), Listeria monocytogenes ATCC 19117 (≥6 mm), Staphylococcus aureus ATCC 8702 (≥3 mm), and the fungi Penicillium nordicum MUM 08.16 (inhibition ≥ 64.8%). In addition, the strains showed the ability to adsorb the mycotoxins aflatoxin B1 (≥40%) and ochratoxin A (≥34%). These results indicate that LAB strains from naturally fermented Brazilian table olives are potentially probiotic and antifungal candidates that can be used for food biopreservation.This research was funded by the Brazilian agencies Conselho Nacional de Desenvolvimento Científico e Tecnológico do Brasil (CNPq) [423095/2016-1; 309561/2019-1], Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) [CAG-APQ-03478-16], Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) [PNPD20131289], Portuguese Foundation for Science and Technology (FCT) [UIDB/04469/2020], LABBELS—Associate Laboratory in Biotechnology, Bioengineering and Microelectromechnaical Systems [LA/P/0029/2020]. Luís Abrunhosa acknowledges FCT for the assistant research contract CEECIND/00728/2017.info:eu-repo/semantics/publishedVersio

Trypsin purification using magnetic particles of azocasein-iron composite

This work presents an inexpensive, simple and fast procedure to purify trypsin based on affinity binding with ferromagnetic particles of azocasein composite (mAzo). Crude extract was obtained from intestines of fish Nile tilapia (Oreochromis niloticus) homogenized in buffer (01 g tissue/ml). This extract was exposed to 100mg of mAzo and washed to remove unbound proteins by magnetic field. Trypsin was leached off under high ionic strength (3 M NaCl). Preparation was achieved containing specific activity about 60 times higher than that of the crude extract. SDS-PAGE showed that the purified protein had molecular weight (24 kDa) in concordance with the literature for the Nile tilapia trypsin. The mAzo composite can be reused and applied to purify trypsin from other sources.Authors are thankful to CNPq (grant 307273/2013-0) and CAPES (Scholarship to MHMEA) Brazil agencies as well as FCT (Scholarship to SICS and CN) Portugal Agencie

Production and characterization of collagenase by Penicillium sp. UCP 1286 isolated from Caatinga soil

A new Penicillium sp. strain isolated from the soil of Caatinga, a Brazilian Biome (UCP 1286) was selected for collagenase production. Fermentation system allowing obtention of collagenolytic activity about 2.7 times higher than existing data, with the highest values of collagenolytic and specific activity (379.80 U/mL, 1460.77 U/mg, respectively), after 126 hours. Applying a factorial design, enzyme production was increased by about 65% compared to the preliminary results. The factorial design demonstrated the existence of two factors with statistical significance on the production of the enzyme: pH and temperature, both with negative effects. Enzyme was found to be more active at pH 9.0 and 37 °C, and also to be very stable in comparison with the collagenase produced by other microorganisms. The enzyme seems to belong to collagenolytic serine proteases family. Concerning the substrate specificity, it was observed that the highest enzyme activity corresponds to azocoll, there was no relevant activity on azocasein and the enzyme showed to be more specific to type V collagen and gelatin than the commercial colagenase produced by Clostridium histolyticum. Major band observed at electrophoresis was approximately 37 kDa. Zymogram analysis confirmed the collagenolytic activity. All data indicates this enzyme as promising biotechnology product.This work was supported by Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE) (IBPG-0137-2.08/12) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). Sara Silvério also acknowledges her post-doc grant (SFRH/BPD/88584/2012) from FCT (Fundação para a Ciência e a Tecnologia), Portugal