Evaluation of a novel saliva-based epidermal growth factor receptor mutation detection for lung cancer: A pilot study.

BackgroundThis article describes a pilot study evaluating a novel liquid biopsy system for non-small cell lung cancer (NSCLC) patients. The electric field-induced release and measurement (EFIRM) method utilizes an electrochemical biosensor for detecting oncogenic mutations in biofluids.MethodsSaliva and plasma of 17 patients were collected from three cancer centers prior to and after surgical resection. The EFIRM method was then applied to the collected samples to assay for exon 19 deletion and p.L858 mutations. EFIRM results were compared with cobas results of exon 19 deletion and p.L858 mutation detection in cancer tissues.ResultsThe EFIRM method was found to detect exon 19 deletion with an area under the curve (AUC) of 1.0 in both saliva and plasma samples in lung cancer patients. For L858R mutation detection, the AUC of saliva was 1.0, while the AUC of plasma was 0.98. Strong correlations were also found between presurgery and post-surgery samples for both saliva (0.86 for exon 19 and 0.98 for L858R) and plasma (0.73 for exon 19 and 0.94 for L858R).ConclusionOur study demonstrates the feasibility of utilizing EFIRM to rapidly, non-invasively, and conveniently detect epidermal growth factor receptor mutations in the saliva of patients with NSCLC, with results corresponding perfectly with the results of cobas tissue genotyping

A Balanced Feed Filtering Antenna With Novel Coupling Structure for Low-Sidelobe Radar Applications

A fourth-order filtering patch antenna with a novel coupling structure is presented in this paper. Using the proposed coupling structure, both the balanced coupling feed and cross-coupling are realized. Two identical slots etched on the ground plane are utilized to excite the radiating patch with the reduced cross-polarization level. A short slot etched on the ground plane is employed for cross-coupling, which introduces two controllable radiation nulls with a steep roll-off rate. In addition, owing to the split-ring resonators and hairpin resonators, the improved impedance bandwidth is achieved with the fourth-order filtering response. To demonstrate the proposed design techniques, both the filtering antenna element and the low-sidelobe array are designed, fabricated, and measured. The measured results show that the proposed antenna has the impedance bandwidth of 12% (4.78–5.39 GHz) with the total height of 0.06?0 , the cross-polarization level lower than ?31 dB, and two radiation nulls with the suppression higher than 31 dB. For the low-sidelobe antenna array, wide impedance bandwidth is also obtained with the sidelobe level below ?28.7 dB, the cross-polarization level below ?34 dB, and the out-of-band suppression better than 25 dB

Tirofiban for Stroke without Large or Medium-Sized Vessel Occlusion

The effects of the glycoprotein IIb/IIIa receptor inhibitor tirofiban in patients with acute ischemic stroke but who have no evidence of complete occlusion of large or medium-sized vessels have not been extensively studied. In a multicenter trial in China, we enrolled patients with ischemic stroke without occlusion of large or medium-sized vessels and with a National Institutes of Health Stroke Scale score of 5 or more and at least one moderately to severely weak limb. Eligible patients had any of four clinical presentations: ineligible for thrombolysis or thrombectomy and within 24 hours after the patient was last known to be well; progression of stroke symptoms 24 to 96 hours after onset; early neurologic deterioration after thrombolysis; or thrombolysis with no improvement at 4 to 24 hours. Patients were assigned to receive intravenous tirofiban (plus oral placebo) or oral aspirin (100 mg per day, plus intravenous placebo) for 2 days; all patients then received oral aspirin until day 90. The primary efficacy end point was an excellent outcome, defined as a score of 0 or 1 on the modified Rankin scale (range, 0 [no symptoms] to 6 [death]) at 90 days. Secondary end points included functional independence at 90 days and a quality-of-life score. The primary safety end points were death and symptomatic intracranial hemorrhage. A total of 606 patients were assigned to the tirofiban group and 571 to the aspirin group. Most patients had small infarctions that were presumed to be atherosclerotic. The percentage of patients with a score of 0 or 1 on the modified Rankin scale at 90 days was 29.1% with tirofiban and 22.2% with aspirin (adjusted risk ratio, 1.26; 95% confidence interval, 1.04 to 1.53, P = 0.02). Results for secondary end points were generally not consistent with the results of the primary analysis. Mortality was similar in the two groups. The incidence of symptomatic intracranial hemorrhage was 1.0% in the tirofiban group and 0% in the aspirin group. In this trial involving heterogeneous groups of patients with stroke of recent onset or progression of stroke symptoms and nonoccluded large and medium-sized cerebral vessels, intravenous tirofiban was associated with a greater likelihood of an excellent outcome than low-dose aspirin. Incidences of intracranial hemorrhages were low but slightly higher with tirofiban

Methylprednisolone as Adjunct to Endovascular Thrombectomy for Large-Vessel Occlusion Stroke

Importance It is uncertain whether intravenous methylprednisolone improves outcomes for patients with acute ischemic stroke due to large-vessel occlusion (LVO) undergoing endovascular thrombectomy. Objective To assess the efficacy and adverse events of adjunctive intravenous low-dose methylprednisolone to endovascular thrombectomy for acute ischemic stroke secondary to LVO. Design, Setting, and Participants This investigator-initiated, randomized, double-blind, placebo-controlled trial was implemented at 82 hospitals in China, enrolling 1680 patients with stroke and proximal intracranial LVO presenting within 24 hours of time last known to be well. Recruitment took place between February 9, 2022, and June 30, 2023, with a final follow-up on September 30, 2023.InterventionsEligible patients were randomly assigned to intravenous methylprednisolone (n = 839) at 2 mg/kg/d or placebo (n = 841) for 3 days adjunctive to endovascular thrombectomy. Main Outcomes and Measures The primary efficacy outcome was disability level at 90 days as measured by the overall distribution of the modified Rankin Scale scores (range, 0 [no symptoms] to 6 [death]). The primary safety outcomes included mortality at 90 days and the incidence of symptomatic intracranial hemorrhage within 48 hours. Results Among 1680 patients randomized (median age, 69 years; 727 female [43.3%]), 1673 (99.6%) completed the trial. The median 90-day modified Rankin Scale score was 3 (IQR, 1-5) in the methylprednisolone group vs 3 (IQR, 1-6) in the placebo group (adjusted generalized odds ratio for a lower level of disability, 1.10 [95% CI, 0.96-1.25]; P = .17). In the methylprednisolone group, there was a lower mortality rate (23.2% vs 28.5%; adjusted risk ratio, 0.84 [95% CI, 0.71-0.98]; P = .03) and a lower rate of symptomatic intracranial hemorrhage (8.6% vs 11.7%; adjusted risk ratio, 0.74 [95% CI, 0.55-0.99]; P = .04) compared with placebo. Conclusions and Relevance Among patients with acute ischemic stroke due to LVO undergoing endovascular thrombectomy, adjunctive methylprednisolone added to endovascular thrombectomy did not significantly improve the degree of overall disability.Trial RegistrationChiCTR.org.cn Identifier: ChiCTR210005172

Improving the Effect of Digital Live Broadcasting Activities Based on Configuration Analysis

[Purpose/Significance] Digital live streaming is an important way for enterprises to carry out online activities. Incorporating clear set qualitative comparative analysis into the research on improving the effectiveness of enterprise digital live streaming activities is an innovation in the application of configuration analysis methods and a new breakthrough in the field of improving activity effectiveness. By identifying elements or combinations of elements that can create greater value for enterprise digital live streaming activities, and adopting various ways to enhance these element combinations, the goal of improving the effectiveness of enterprise digital live streaming activities can be achieved, which has innovative guiding significance for enterprise development. [Method/Process] Based on the consideration of sample homogeneity, the background data of the digital live streaming activity of Guangxi Zhongyan Industry Co., Ltd. was used as the data source. Four influencing factors related to the digital live streaming activity were selected as conditional variables using literature induction and problem oriented methods, and some conditional variables were assigned values using linear discriminant dimensionality reduction. At the same time, the activity effect was used as the outcome variable, configuration analysis was conducted on digital live streaming activities in the tobacco industry through clear set qualitative comparative analysis, and combined with SOR model and the 4I theory, a configuration path for improving the effectiveness was generated. [Conclusions/Results] Research has found that a single factor does not constitute a necessary condition for improving the effectiveness of digital live streaming activities. There are two configuration paths: game assisted and topic supported, which can improve the effectiveness of digital live streaming activities. The mechanism of action can be summarized as follows: rich topic types are conducive to meeting users' curiosity psychology, topic popularity and game quantity can ensure users' participation in live streaming, and user interaction work is the basic guarantee for improving user retention. Meanwhile, these two paths can be applied to different forms of digital live streaming, and enterprises should choose according to their own needs and refine the paths in practice. In addition, although this article explores the key paths to improve the effectiveness of digital live streaming activities, due to limitations in research samples and industry fields, it has not fully revealed the various factors that affect the effectiveness of digital live streaming, and there is a certain degree of subjectivity in the criteria for assigning variables. Therefore, further revisions and improvements are needed in future research

Feature Evaluation and Comparison in Radar Emitter Recognition Based on SAHP

In the field of radar emitter recognition, with the wide application of modern radar, the traditional recognition method based on typical five feature parameters cannot achieve satisfactory recognition results in a complex electromagnetic environment. Currently, many new feature extraction methods are presented, but few approaches have been applied for feature evaluation or performance comparison. To deal with this problem, a feature evaluation and selection method was proposed based on set pair analysis (SPA) theory and analytic hierarchy process (AHP). The main idea of this method is to use SPA theory to solve problems regarding the construction of the decision matrix based on AHP, as it relies heavily on expert’s subjective experience. The aim was to improve the objectivity of the evaluation. To check the effectiveness of the proposed method, six feature parameters were selected for a comprehensive performance evaluation. Then, the convolutional neural network (CNN) was introduced to validate the recognition capability based on the evaluation results. Simulation results demonstrated that the proposed method could achieve the feature analysis and evaluation more reasonably and objectively