Uber die Legierungen des Mangans und Siliziums mit Alkali- und Erdalkalimetallen

Bekanntlich uben die Zusatze von Alkali- und Erdalkalimetallen wie Kalzium, Lithium, Barium und Strontium zu Eisenschmelzen vor allem zur Veredelung des Gusseisens durch Kugelgraphitstruktur eine beachtliche Wirkung aus. Auch wegen ihrer starken Affinitat fur Gase in der Schmelze hieraus folgt eine interessante Moglichkeit der Eigenschaftsverbesserung fur Stahle. Dies wirft die Frage auf, wie diese Metalle zur Eisenschmelze wirksam zugesetzt werden kann. Zu diesem Zweck konnte man vorschlagen, sie in Form einer Legierung mit Mangan sowie Silizium zu verwenden. Die Legierungssysteme des Mangans mit Lithium, Barium und Strontium sind aber bislang gar nicht bearbeitet worden, wahrend das System Mangan-Kalzium^ von den Verfassern kurzlich untersucht worden ist. Uber die Legierungen des Siliziums mit Lithium, Barium und Strontium liegen einige Unter-suchungen vor, die sich hauptsachlich mit der praktischen Darstellung der Silizide und der Bestimmung ihrer Zusammensetzung beschaftigen. Die vol-lstandigen Zustandsiblder dieser Systeme sind heute nicht bekannt. Die Tausache, dass man durch die ublichen Schmelzverfahren keine reine Legierungen herstellen kann, verhindert die Untersuchungen uber diese Legierungssysteme. Die Schwierig-keit ist jedoch, wie in der Arbeit uber das System Mangan-Kalzium^ angegeben, durch Lichtbogenschmelzen in einer wassergekuhlten Kupferkokille unter Argon gelost worden

Novel parasitic chytrids infecting snow algae in an alpine snow ecosystem in Japan

IntroductionMicrobial communities are important components of glacier and snowpack ecosystems that influence biogeochemical cycles and snow/ice melt. Recent environmental DNA surveys have revealed that chytrids dominate the fungal communities in polar and alpine snowpacks. These could be parasitic chytrids that infect snow algae as observed microscopically. However, the diversity and phylogenetic position of parasitic chytrids has not been identified due to difficulties in establishing their culture and subsequent DNA sequencing. In this study, we aimed to identify the phylogenetic positions of chytrids infecting the snow algae, Chloromonas spp., bloomed on snowpacks in Japan.MethodsBy linking a microscopically picked single fungal sporangium on a snow algal cell to a subsequent sequence of ribosomal marker genes, we identified three novel lineages with distinct morphologies.ResultsAll the three lineages belonged to Mesochytriales, located within “Snow Clade 1”, a novel clade consisting of uncultured chytrids from snow-covered environments worldwide. Additionally, putative resting spores of chytrids attached to snow algal cells were observed.DiscussionThis suggests that chytrids may survive as resting stage in soil after snowmelt. Our study highlights the potential importance of parasitic chytrids that infect snow algal communities

ゲノム配列解析ツール giv の開発

We here describe gve, a new CUI tool for analyzing genome sequence

Early mortality factors in immune checkpoint inhibitor monotherapy for advanced or metastatic non‑small cell lung cancer

Purpose: Immune checkpoint inhibitors (ICI) are a promising treatment, but may cause hyperprogressive disease and early death. The present study investigated early mortality factors in ICI monotherapy for lung cancer. Patients and methods: We retrospectively reviewed all patients diagnosed with advanced or metastatic non-small cell lung cancer (NSCLC) and treated with ICI monotherapy (nivolumab, pembrolizumab, and atezolizumab) between March 2016 and August 2021 at National Hospital Organization Kochi Hospital and Tokushima University. Early death was defined as patients who died within 60 days of ICI treatment. Results: A total of 166 patients were included. The majority of patients (87%) had an Eastern cooperative oncology group (ECOG) Performance status (PS) of 0/1. There were 21 early deaths. Significant differences were observed in ECOG PS, the histological type, liver metastasis, tumor size, the white blood cell count, neutrophils (%), lymphocytes (%), the neutrophilto-lymphocyte ratio in serum (sNLR), C-reactive protein (CRP), and albumin between the groups with or without early death. Univariate logistic regression analyses identified ECOG PS score ≥ 2, liver metastasis, tumor size ≥ 5 cm, neutrophils ≥ 69%, lymphocytes < 22%, sNLR ≥ 4, CRP ≥ 1 mg/dl, and albumin < 3.58 g/dl as significant risk factors for early death. A multivariate logistic regression analysis revealed that liver metastasis (Odds ratio [OR], 10.3; p = 0.008), ECOG PS score ≥ 2 (OR, 8.0; p = 0.007), and a smoking history (OR, 0.1; p = 0.03) were significant risk factors for early death. Conclusion: Liver metastases, ECOG PS score ≥ 2, and a non-smoking history are early mortality factors in ICI monotherapy for advanced or metastatic NSCLC

Utility of Combined Use of Transabdominal Ultrasonography and Fecal Immunochemical Test Examinations in Ulcerative Colitis

This study examined the utility of the combined use of transabdominal ultrasonography (TUS) and fecal immunochemical testing (FIT) to detect mucosal inflammation, vis-a-vis the Mayo endoscopic subscore (MES), in ulcerative colitis (UC). Sixty-three UC patients who underwent TUS and FIT were retrospectively enrolled. For TUS, the colon was divided into five segments, and the bowel wall thickness was measured and evaluated. The accuracy of FIT (> 100 ng/ml) in detecting mucosal inflammation (MES>0) was 0.93, whereas that of TUS (BWT>2 mm) in each segment was 0.84-0.97. The combined use of TUS and FIT may be helpful in noninvasive treatment strategies

Insurance value of biodiversity in the Anthropocene is the full resilience value

Recently two distinctly different conceptualisations of insurance value of biodiversity/ ecosystems have been developed. The ecosystem framing addresses the full resilience value without singling out subjective risk preferences. Conversely, the economic framing focuses exactly on this subjective value of risk aversion, implying that the insurance value is zero for risk neutral persons. Here we analyse the differences conceptually and empirically, and relate this to the broader socio-cultural dimensions of social-ecological resilience. The uncertainty of the Anthropocene blurs the distinction between subjective/objective. We show that the economic framing has been operationalised only in specific cases while the broader literature on resilience, disaster risk reduction, and nature-based solutions tend to address the full value of resilience. Yet, the empirical literature that relates to insurance value of biodiversity is hardly consistent with resilience theory because the slow underlying variables defining resilience are rarely addressed. We suggest how the empirical literature on insurance value can be better aligned with resilience theory. Since the ecosystem framing of insurance value captures the essence of the resilience, we propose using the concept “resilience value” as it may reduce the present ambiguity in terminology and conceptualisation of insurance value of biodiversity. nsurance value of ecosystems Natural insurance value Ecosystem services General resilience Specified resiliencepublishedVersio

Functional Sperm of the Yellowtail (Seriola quinqueradiata) Were Produced in the Small-Bodied Surrogate, Jack Mackerel (Trachurus japonicus).

Production of xenogeneic gametes from large-bodied, commercially important marine species in closely related smaller surrogates with short generation times may enable rapid domestication of the targeted species. In this study we aimed to produce gametes of Japanese yellowtail (Seriola quinqueradiata) using jack mackerel (Trachurus japonicus) as a surrogate with a smaller body size and shorter maturation period. Donor spermatogonia were collected from the testes of yellowtail males and transferred into the peritoneal cavity of 10- and 12-day-old jack mackerel larvae. Twenty days later, 59.5% of the recipients survived of which 88.2% had donor-derived germ cells in their gonads. One year later, genomic DNA templates were prepared from the semen of 96 male recipients and subjected to PCR analyses using primers specific for the yellowtail vasa sequence, resulting in the detection of positive signals in semen from two recipients. The milt collected from the recipients was used for fertilization with yellowtail eggs. Of eight hatchlings obtained from the crosses, two were confirmed to be derived from donor yellowtail by DNA markers, although the others were gynogenetic diploids. These findings indicate that it is possible to produce donor-derived sperm in xenogeneic recipients with smaller body size and shorter generation time by transplanting spermatogonia. Thus, the xenogeneic transplantation of spermatogonia might be a potential tool to produce gametes of large-bodied, commercially important fish although the efficiency of the method requires further improvement. This is the first report demonstrating that donor-derived sperm could be produced in xenogeneic recipient via spermatogonial transplantation in carangid fishes

Molecular database for classifying Shorea species (Dipterocarpaceae) and techniques for checking the legitimacy of timber and wood products

The extent of tropical forest has been declining, due to over-exploitation and illegal logging activities. Large quantities of unlawfully extracted timber and other wood products have been exported, mainly to developed countries. As part of the export monitoring effort, we have developed methods for extracting and analyzing DNA from wood products, such as veneers and sawn timbers made from dipterocarps, in order to identify the species from which they originated. We have also developed a chloroplast DNA database for classifying Shorea species, which are both ecologically and commercially important canopy tree species in the forests of Southeast Asia. We are able to determine the candidate species of wood samples, based on DNA sequences and anatomical data. The methods for analyzing DNA from dipterocarp wood products may have strong deterrent effects on international trade of illegitimate dipterocarp products. However, the method for analyzing DNA from wood is not perfect for all wood products and need for more improvement, especially for plywood sample. Consequently, there may be benefits for the conservation of tropical forests in Southeast Asia. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10265-010-0348-z) contains supplementary material, which is available to authorized users

Double-Stranded RNA of Intestinal Commensal but Not Pathogenic Bacteria Triggers Production of Protective Interferon-β

SummaryThe small intestine harbors a substantial number of commensal bacteria and is sporadically invaded by pathogens, but the response to these microorganisms is fundamentally different. We identified a discriminatory sensor by using Toll-like receptor 3 (TLR3). Double-stranded RNA (dsRNA) of one major commensal species, lactic acid bacteria (LAB), triggered interferon-β (IFN-β) production, which protected mice from experimental colitis. The LAB-induced IFN-β response was diminished by dsRNA digestion and treatment with endosomal inhibitors. Pathogenic bacteria contained less dsRNA and induced much less IFN-β than LAB, and dsRNA was not involved in pathogen-induced IFN-β induction. These results identify TLR3 as a sensor to small intestinal commensal bacteria and suggest that dsRNA in commensal bacteria contributes to anti-inflammatory and protective immune responses