Immunogenic Salivary Proteins of Triatoma infestans: Development of a Recombinant Antigen for the Detection of Low-Level Infestation of Triatomines

Chagas disease, caused by Trypanosoma cruzi, is a neglected disease with 20 million people at risk in Latin America. The main control strategies are based on insecticide spraying to eliminate the domestic vectors, the most effective of which is Triatoma infestans. This approach has been very successful in some areas. However, there is a constant risk of recrudescence in once-endemic regions resulting from the re-establishment of T. infestans and the invasion of other triatomine species. To detect low-level infestations of triatomines after insecticide spraying, we have developed a new epidemiological tool based on host responses against salivary antigens of T. infestans. We identified and synthesized a highly immunogenic salivary protein. This protein was used successfully to detect differences in the infestation level of T. infestans of households in Bolivia and the exposure to other triatomine species. The development of such an exposure marker to detect low-level infestation may also be a useful tool for other disease vectors

Assessing Tuberculosis Case Fatality Ratio: A Meta-Analysis

Background: Recently, the tuberculosis (TB) Task Force Impact Measurement acknowledged the need to review the assumptions underlying the TB mortality estimates published annually by the World Health Organization (WHO). TB mortality is indirectly measured by multiplying estimated TB incidence with estimated case fatality ratio (CFR). We conducted a meta-analysis to estimate the TB case fatality ratio in TB patients having initiated TB treatment. Methods: We searched for eligible studies in the PubMed and Embase databases through March 4(th) 2011 and by reference listing of relevant review articles. Main analyses included the estimation of the pooled percentages of: a) TB patients dying due to TB after having initiated TB treatment and b) TB patients dying during TB treatment. Pooled percentages were estimated using random effects regression models on the combined patient population from all studies. Main Results: We identified 69 relevant studies of which 22 provided data on mortality due to TB and 59 provided data on mortality during TB treatment. Among HIV infected persons the pooled percentage of TB patients dying due to TB was 9.2% (95% Confidence Interval (CI): 3.7%-14.7%) and among HIV uninfected persons 3.0% (95% CI: 21.2%-7.4%) based on the results of eight and three studies respectively providing data for this analyses. The pooled percentage of TB patients dying during TB treatment was 18.8% (95% CI: 14.8%-22.8%) among HIV infected patients and 3.5% (95% CI: 2.0%-4.92%) among HIV uninfected patients based on the results of 27 and 19 studies respectively. Conclusion: The results of the literature review are useful in generating prior distributions of CFR in countries with vital registration systems and have contributed towards revised estimates of TB mortality This literature review did not provide us with all data needed for a valid estimation of TB CFR in TB patients initiating TB treatmen

Feeding-Entrained Circadian Rhythms in Fishes

There has been sufficient work done in fishes to warrant a review of feeding-entrained rhythmicity, if only for comparative reasons. Relative to the extensive work with laboratory mammals, however, information on this topic in fishes is meager. An attempt at a broad overview at this point is fated from the outset to be data poor and speculation rich; hopefully this paper will, nevertheless, serve a heuristic role. Specifically, the paper addresses five questions regarding feeding entrainment of circadian rhythms in fishes: Does feeding entrain rhythms in fishes? How? Why? So what? and What next?https://nsuworks.nova.edu/occ_facbooks/1020/thumbnail.jp

The All-Wavelength Extended Groth Strip International Survey (AEGIS) data sets

In this the first of a series of Letters, we present a panchromatic data set in the Extended Groth Strip region of the sky. Our survey, the All-Wavelength Extended Groth Strip International Survey (AEGIS), aims to study the physical properties and evolutionary processes of galaxies at z ∼ 1. It includes the following deep, wide-field imaging data sets: Chandra/ACIS X-ray, GALEX ultraviolet, CFHT/MegaCam Legacy Survey optical, CFHT/CFH12K optical, Hubble Space Telescope/ACS optical and NICMOS near-infrared, Palomar/WIRC near-infrared, Spitzer/IRAC mid-infrared, Spitzer/MIPS far-infrared, and VLA radio continuum. In addition, this region of the sky has been targeted for extensive spectroscopy using the Deep Imaging Multi-Object Spectrograph (DEIMOS) on the Keck II 10 m telescope. Our survey is compared to other large multiwavelength surveys in terms of depth and sky coverage. © 2007. The American Astronomical Sociey, All rights reserved

Molecular insights into the ligand-controlled organization of SAM-I riboswitch

S-adenosylmethionine (SAM) riboswitches are widespread in bacteria, and up to five different SAM riboswitch families have been reported, highlighting the relevance of SAM regulation. On the basis of crystallographic and biochemical data, it has been postulated, but never demonstrated, that ligand recognition by SAM riboswitches involves key conformational changes in the RNA architecture. We show here that the aptamer follows a two-step hierarchical folding selectively induced by metal ions and ligand binding, each of them leading to the formation of one of the two helical stacks observed in the crystal structure. Moreover, we find that the anti-antiterminator P1 stem is rotated along its helical axis upon ligand binding, a mechanistic feature that could be common to other riboswitches. We also show that the nonconserved P4 helical domain is used as an auxiliary element to enhance the ligand-binding affinity. This work provides the first comprehensive characterization, to our knowledge, of a ligand-controlled riboswitch folding pathway