Análise numérica tridimensional de blocos sobre duas estacas

This paper compares the results between a non-linear three-dimensional numerical analysis of pile caps with two piles and the experimental study conducted by Delalibera. It is verified the load-carrying capacity, the crack pattern distribution, the principal stress in concrete and steel, the deflection and the fracture of the pile cap. The numerical analysis is executed with the finite-element software ATENA 3D, considering a perfect bond between concrete and steel. The numerical and experimental results are presented and have demonstrated a good approximation, reasserting the results of the experimental model and corroborating the theory.Neste artigo é realizado um estudo comparativo entre o comportamento experimental de blocos de concreto armado apoiados sobre duas estacas, a partir de modelo experimental de Delalibera, e o obtido a partir de análise numérica tridimensional não-linear. A comprovação de resultados é feita observando-se a força última, o panorama de fissuração, o fluxo de tensões atuantes no concreto e no aço, as deformações e o padrão de ruína. A simulação numérica do comportamento estrutural do bloco de concreto armado é realizada com o emprego do programa computacional de elementos finitos ATENA 3D, considerando a aderência perfeita entre as barras de aço da armadura e o concreto. Os resultados numéricos e experimentais são apresentados e quando comparados apresentam uma boa aproximação, comprovando os fundamentos teóricos e os resultados experimentais.252283Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES

The cooperative action of CSB, CSA, and UVSSA target TFIIH to DNA damage-stalled RNA polymerase II

The response to DNA damage-stalled RNA polymerase II (RNAPIIo) involves the assembly of the transcription-coupled repair (TCR) complex on actively transcribed strands. The function of the TCR proteins CSB, CSA and UVSSA and the manner in which the core DNA repair complex, including transcription factor IIH (TFIIH), is recruited are largely unknown. Here, we define the assembly mechanism of the TCR complex in human isogenic knockout cells. We show that TCR is initiated by RNAPIIo-bound CSB, which recruits CSA through a newly identified CSA-interaction motif (CIM). Once recruited, CSA facilitates the association of UVSSA with stalled RNAPIIo. Importantly, we find that UVSSA is the key factor that recruits the TFIIH complex in a manner that is stimulated by CSB and CSA. Together these findings identify a sequential and highly cooperative assembly mechanism of TCR proteins and reveal the mechanism for TFIIH recruitment to DNA damage-stalled RNAPIIo to initiate repair. The response to DNA damage-stalled RNA polymerase II leads to the assembly of the transcription-coupled repair (TCR) complex on actively transcribed strands. Here, the authors reveal the complex assembly mechanism of the TCR complex in human cells.Genome Instability and Cance

ELOF1 is a transcription-coupled DNA repair factor that directs RNA polymerase II ubiquitylation

Two side-by-side papers report that the transcription elongation factor ELOF1 drives transcription-coupled repair and prevents replication stress.Cells employ transcription-coupled repair (TCR) to eliminate transcription-blocking DNA lesions. DNA damage-induced binding of the TCR-specific repair factor CSB to RNA polymerase II (RNAPII) triggers RNAPII ubiquitylation of a single lysine (K1268) by the CRL4(CSA) ubiquitin ligase. How CRL4(CSA) is specifically directed towards K1268 is unknown. Here, we identify ELOF1 as the missing link that facilitates RNAPII ubiquitylation, a key signal for the assembly of downstream repair factors. This function requires its constitutive interaction with RNAPII close to K1268, revealing ELOF1 as a specificity factor that binds and positions CRL4(CSA) for optimal RNAPII ubiquitylation. Drug-genetic interaction screening also revealed a CSB-independent pathway in which ELOF1 prevents R-loops in active genes and protects cells against DNA replication stress. Our study offers key insights into the molecular mechanisms of TCR and provides a genetic framework of the interplay between transcriptional stress responses and DNA replication.Cancer Signaling networks and Molecular Therapeutic

Making Self-directed learning work

Contains fulltext : 226617.pdf (publisher's version ) (Open Access)Radboud University, 09 december 2020Promotores : Wilt, G.J. van der, Jaarsma, A.D.C. Co-promotores : Reuzel, R.P.B., Koksma, J.J

Three-dimensional analysis of two-pile caps

This paper compares the results between a non-linear three-dimensional numerical analysis of pile caps with two piles and the experimental study conducted by Delalibera. It is verified the load-carrying capacity, the crack pattern distribution, the principal stress in concrete and steel, the deflection and the fracture of the pile cap. The numerical analysis is executed with the finite-element software ATENA 3D, considering a perfect bond between concrete and steel. The numerical and experimental results are presented and have demonstrated a good approximation, reasserting the results of the experimental model and corroborating the theory

Development and testing of a magnetically actuated capsule endoscopy for obesity treatment

10.1371/journal.pone.0148035PLoS ONE111e014803