Serum glycomarkers of endoplasmic reticulum and lysosomal-endosomal system stress in human healthy aging and diseases

To verify the idea that extracellular free oligosaccharides might be able to reflect the functional status of the endoplasmic reticulum (ER) and lysosomal-endosomal system, HPLC-profiles of serum-derived free oligosaccharides (FOS) in human healthy aging, acute myeloproliferative neoplasms, and cardiovascular pathologies were compared with intracellular glycans. After plasma deproteinization and FOS purification the oligosaccharides were labelled with anthranilic acid, separated into the neutral and charged with QAE Sephadex (Q25-120) chromatography and analysed using high-performance liquid chromatography (HPLC). The charged FOS were digested with a sialidase and compared with free oligosaccharides from transferrin for structural decoding. HPLC-profiles of serum-derived FOS revealed mild delay of the dolichol phosphate cycle in ER, moderate intensification of ER-associated degradation (ERAD) and degradation in endosomallysosomal system with aging; an inhibition of the dolichol phosphate cycle, intensification of ERAD and increasing of lysosomal exocytosis in acute myeloproliferative neoplasms; intensification of ERAD and glycocojugate degradation with endosomal-lysosomal system in cardiovascular diseases. As serum free oligosaccharides are able to reflect specifically perturbations in ER and endosomal-lysosomal system under wide range of stressors they can serve as extracellular markers of functionality of these organelles

Ligand-affinity chromatographic purification of #alpha#-fucosidases and #alpha#-glucosidases Tools for the structural characterisation of N-linked oligosaccharides from diverse species

SIGLEAvailable from British Library Document Supply Centre-DSC:DX190836 / BLDSC - British Library Document Supply CentreGBUnited Kingdo

Detection of the lipid-linked precursor oligosaccharide of N-linked protein glycosylation in Drosophila melanogaster

AbstractThe presence of a glycan of the same molecular size as the lipid linked precursor oligosaccharide (Glc3Man9GlcNAc2) of the N-linked protein glycosylation pathway in mammalian cells has been detected in a glycolipid fraction of cultured Drosophila melanogaster cells. Oligosaccharide sequencing studies were consistent with the existence of a glucosylated high mannose containing structure, which may be the common precursor for N-linked protein glycosylation in insect cells

The glycomic effect of N-acetylglucosaminyltransferase III overexpression in metastatic melanoma cells. GnT-III modifies highly branched N-glycans

Proteomic

Diagnosis of atrial ectopic origin from the body surface ECG : Insights from 3D virtual human atria and torso

Ectopic atrial activity underlies atrial tachycardia (AT), which, may predispose to atrial fibrillation (AF). Such abnormal excitation may be reflected as an alteration in the P-wave morphology (PWM) of the body surface potential (BSP). Identifying the location of the ectopic foci from body surface potential maps, can help to diagnose the early onset of AF in a cost effective manner. However, the correlation between the PWM of BSP and the origin of atrial foci has not been well established. In this study we use a biophysically detailed computational model of the human atria and torso to investigate such correlations