Evaluation Of Rat Testes Treated With Arctium Lappa L: Morphometric Study

Arctium lappa L. (burdock) is a plant brought from Japan and acclimated in Brazil, which is widely used in popular medicine all over the world. This study was carried out to assess the possible effects of A. lappa in testes of adult Wistar rats. They received the extract in water bottles at doses of 10 or 20 g/L daily for 40 days. There were no significant alterations in the biometric data including body and accessory sexual organs weight and in the histometric data such as volume density of the testis compartments and Leydig cell morphometry. Unaltered histometric and biometric data shows that A. lappa did not cause impairment of spermatogenesis.242112117Capasso, R., Izzo, A.A., Pinto, L., Bifulco, T., Vitobello, C., Mascolo, N., Phytotherapy and quality of herbal medicines (2000) Fitoterapia, 71, pp. 58-65Chen, F., Wu, A., Chen, C., The influence of different treatments on the free radical scavenging activity of burdock and variations of its active components (2004) Food Chem, 86, pp. 479-484Creasy, D.M., Pathogenesis of Male Reproductive Toxicity (2001) Toxicol. Pathol, 29, pp. 64-76França, L.R., Godinho, C.L., Testis morphometry, seminiferous epithelium cycle lengh, and daily sperm production in domestic cats (Felis catus) (2003) Biol. Reprod, 68, pp. 1556-1561França, L.R., Russell, L.D., The testes of domestic animals (1998) Male Reproduction. a Multidisciplinary Overview, pp. 197-219. , Regadera J, Martinez-Garcia, eds, Churchill Livingstone: MadridKim, I., Yang, H., Morphometric study of the testicular interstitium of rats during postnatal development. The korean (1999) J. Anat, 32, pp. 849-858Franco, I.F., Fontana, V.L., Ervas & Plantas (2002) A Medicina Dos Simples, p. 208. , Livraria Vida, EreximLeonard, S.S., Keil, D., Mehlman, T., Proper, S., Shi, X., Harris, G.K., Essiac tea: Scavenging of reactive oxygen species and effects on DNA damage (2006) J. Ethnopharmacol, 103, pp. 288-296Lin, C.C., Lu, J.M., Yang, J.J., Chuang, S.C., Ujiie, T., Anti-inflammatory and radical scavenge effects of Arctium lappa (1996) Am. J. Chin. Med, 24, pp. 127-137Lin, S.C., Chung, T.C., Lin, C.C., Ueng, T.H., Lin, Y.H., Lin, S.Y., Wang, L.Y., Hepatoprotective effects of Arctium lappa on carbon tetrachloride- and acetaminophen- induced liver damage (2000) A. J. Chin. Med, 28, pp. 163-173Lin, S.C., Lin, C.H., Lin, C.C., Lin, Y.H., Chen, C.F., Chen, I.C., Wang, L.Y., Hepatoprotective effects of Arctium lappa Linne on liver injuries induced by chronic ethanol consumption and potentiated by 10 carbon tetrachloride (2002) J. Biomed. Sci, 9, pp. 401-409Mori, H., Christensen, A.K., Morphometric analysis of Leydig cells in the normal rat testis (1980) J. Cell Biol, 84, pp. 340-354Russell, L.D., Ettlin, R.A., Sinha-Hikim, A.P., Clegg, (1990) Histological and Histopathological Evaluation of the Testis, , Cache River Press: ClearwaterPereira, J.V., Bergamo, D.C.B., Pereira, J.O., França, S.C., Pietro, R.C.L.R., Silva-Souza, Y.T.C., Antimicrobial activity of Arctium lappa constituents against microorganisms commoly found in endodontic infections (2005) Braz. Dent. Journal, 16, pp. 192-196Russell, L.D., França, L.R., Building a testis (1995) Tissue Cell, 27, pp. 129-14

Morfofisiologia do tecido intertubular e das células de Leydig de jaguatirica (Leopardus pardalis) adulta

Estudou-se o espaço intertubular e descreveu-se seu arranjo em jaguatirica adulta (Leopardus pardalis). Para tal, colheram-se fragmentos dos testículos, de cinco jaguatiricas adultas, provenientes de cativeiro, obtidos por biópsia incisional. O compartimento intertubular correspondeu a 19,4% do parênquima, sendo 3,9% ocupado por células de Leydig. Estes se apresentaram uninucleados, com o núcleo arredondado e nucléolo único, e com grande quantidade de grânulos pigmentares no citoplasma. O compartimento intertubular apresentou padrão do tipo II, e o tecido conjuntivo foi o componente mais abundante do tecido intertubular. Observou-se pequeno percentual de células de Leydig na espécie estudada, e o número médio por grama de testículo, 33,39 x106, apresentou-se dentro da amplitude descrita para a maioria dos mamíferos.The aim of this study was to do a quantitative investigation of the intertubular space and describe its arrangement in adult ocelots (Leopardus pardalis). In this experiment we used testicular fragments, obtained through biopsy from five adult ocelots maintained in captivity. The intertubular compartment corresponded to 19.4% of the testicular parenchyma, and 3.9% of this compartment was occupied by Leydig cells. The Leydig cells showed to be uninucleate, with rounded nuclei and single nucleoli, with a large amount of pigment granules in their cytoplasm. The intertubular compartment showed a clear pattern of type II and the conjunctive tissue was the most abundant component of the intertubular tissue. Despite the small unit volume of Leydig cells in adult ocelots, the average number per gram of testis (33.39 x106) was within the range described for most mammals

Espermatogênese de catetos (Tayassu tajacu) Spermatogenesis in the collared peccary (Tayassu tajacu)

Realizaram-se estudos morfológico e funcional da espermatogênese em 15 testículos de catetos adultos, provenientes de abatedouro comercial de animais silvestres. Foram estudados os estádios do ciclo do epitélio seminífero (CES), a população celular dos túbulos seminíferos, o rendimento intrínseco da espermatogênese, o índice das células de Sertoli, o diâmetro médio dos túbulos seminíferos e a proporção volumétrica dos componentes do parênquima testicular. O coeficiente de eficiência de mitoses espermatogoniais foi de 22,54, a eficiência da mitose de 22,54, o rendimento meiótico de 1:2,7 e o rendimento geral da espermatogênese de 1:64. Concluiu-se que a espermatogênese dos catetos é muito semelhante à espermatogênese de suínos.The aim of this study was to analyze morphologically and functionally the spermatogenesis in adult collared peccary. Fifteen testes provided by a commercial wild animal abattoir were used. The stages of the seminiferous epithelium cycle, the cellular number of the seminiferous tubules, the intrinsic efficiency of spermatogenesis, the Sertoli cells, the mean diameter of the seminiferous tubules and the volumetric proportion of testicular parenchyma components were analyzed. The mitosis efficiency coefficient in collared peccary was 22.54, the meiotic profile, 1:2.7, and spermatogenesis general profile was 1:64. It was concluded that spermatogenesis in peccary is very similar to that in pigs