Modified Rate-Theory Predictions in Comparison to Microstructural Data

Standard rate theory methods have recently been combined with experimental microstructures to successfully reproduce measured swelling behavior in ternary steels around 400 C. Fit parameters have reasonable values except possibly for the recombination radius, R{sub c}, which can be larger than expected. Numerical simulations of void nucleation and growth reveal the importance additional recombination processes at unstable clusters. Such extra recombination may reduce the range of possible values for R{sub c}. A modified rate theory is presented here that includes the effect of these undetectably small defect clusters. The fit values for R{sub c} are not appreciably altered, as the modification has little effect on the model behavior in the late steady state. It slightly improves the predictions for early transient times, when the sink strength of stable voids and dislocations is relatively small. Standard rate theory successfully explains steady swelling behavior in high purity stainless steel

Evidence for a companion to BM Gem, a silicate carbon star

Balmer and Paschen continuum emission as well as Balmer series lines of P Cygni-type profile from H_gamma through H_23 are revealed in the violet spectra of BM Gem, a carbon star associated with an oxygen-rich circumstellar shell (`silicate carbon star') observed with the high dispersion spectrograph (HDS) on the Subaru telescope. The blue-shifted absorption in the Balmer lines indicates the presence of an outflow, the line of sight velocity of which is at least 400 km s^-1, which is the highest outflow velocity observed to date in a carbon star. We argue that the observed unusual features in BM Gem are strong evidence for the presence of a companion, which should form an accretion disk that gives rise to both an ionized gas region and a high velocity, variable outflow. The estimated luminosity of ~0.2 (0.03-0.6) L_sun for the ionized gas can be maintained by a mass accretion rate to a dwarf companion of ~10^-8 M_sun yr^-1, while ~10^-10 M_sun yr^-1 is sufficient for accretion to a white dwarf companion. These accretion rates are feasible for some detached binary configurations on the basis of the Bond-Hoyle type accretion process. We concluded that the carbon star BM Gem is in a detached binary system with a companion of low mass and low luminosity. However, we are unable to determine whether this companion object is a dwarf or a white dwarf. The upper limits for binary separation are 210 AU and 930 AU for a dwarf and a white dwarf, respectively. We also note that the observed features of BM Gem mimic those of Mira (omi Cet), which may suggest actual similarities in their binary configurations and circumstellar structures.Comment: 11 pages, 2 figures, 1 table, accepted for publication in Ap

Virus-free induction of pluripotency and subsequent excision of reprogramming factors

Reprogramming of somatic cells to pluripotency, thereby creating induced pluripotent stem (iPS) cells, promises to transform regenerative medicine. Most instances of direct reprogramming have been achieved by forced expression of defined factors using multiple viral vectors1-7. However, such iPS cells contain a large number of viral vector integrations1,8, any one of which could cause unpredictable genetic dysfunction. While c-Myc is dispensable for reprogramming9,10, complete elimination of the other exogenous factors is also desired since ectopic expression of either Oct4 or Klf4 can induce dysplasia11,12. Two transient transfection reprogramming methods have been published to address this issue13,14. However, the efficiency of either approach is extremely low, and neither has thus far been applied successfully to human cells. Here we show that non-viral transfection of a single multiprotein expression vector, which comprises the coding sequences of c-Myc​,​ Klf4​,​ Oct4 and Sox2 linked with 2A peptides, can reprogram both mouse and human fibroblasts. Moreover, the transgene can be removed once reprogramming has been achieved. iPS cells produced with this non-viral vector show robust expression of pluripotency markers, indicating a reprogrammed state confirmed functionally by in vitro differentiation assays and formation of adult chimeric mice. When the single vector reprogramming system was combined with a piggyBac transposon15,16 we succeeded in establishing reprogrammed human cell lines from embryonic fibroblasts with robust expression of pluripotency markers. This system minimizes genome modification in iPS cells and enables complete elimination of exogenous reprogramming factors, efficiently providing iPS cells that are applicable to regenerative medicine, drug screening and the establishment of disease models

Chemical composition of carbon-rich, very metal-poor subgiant LP625-44 observed with the Subaru/HDS

We have obtained a high resolution (R~90,000) spectrum of the carbon- and s-process-element-rich, very metal-poor ([Fe/H]=-2.7) subgiant LP625-44, as well as that of HD140283 (a metal-poor subgiant with normal abundance ratio) for comparison, with the High Dispersion Spectrograph (HDS) for the Subaru Telescope for detailed abundance study. The oxygen abundance derived from the O I triplet around 7770A is uncertain, but the excess of oxygen in LP625-44 seems remarkable (perhaps by nearly a factor 10), in comparison with that of HD140283 derived from the same lines. The Na enhancement in LP625-44 is by about a factor 50, suggesting hydrogen burning in the 22Ne-rich layer in an asymptotic giant branch star which produces the abundance pattern of this object. In our new spectrum of LP625-44, the Pb I lambda 3683A line has been detected, as well as the Pb I lambda 4057A line which has already been studied, confirming the Pb abundance (log epsilon(Pb)~1.9) derived by the previous work. The abundance ratio of s-process elements at the second peak (e.g., La, Ce and Nd) to that at the third peak (Pb) in LP 625-44 is significantly higher (by a factor 5) than that in other three s-process element-rich objects recently studied by van Eck et al.. Recent theoretical works have modeled the s-process nucleosynthesis in the radiative layer of asymptotic giant branch stars in the inter-pulse phase, and the above results means that these processes produced a large scatter in the abundance ratios. Another possiblity is that different processes (e.g., s-process nucleosynthesis during thermal pulses) have contributed to heavy elements in the early Galaxy.Comment: 65 pages, 11 figures, to appear in PAS

Heteroepitaxial growth of ferromagnetic MnSb(0001) films on Ge/Si(111) virtual substrates

Molecular beam epitaxial growth of ferromagnetic MnSb(0001) has been achieved on high quality, fully relaxed Ge(111)/Si(111) virtual substrates grown by reduced pressure chemical vapor deposition. The epilayers were characterized using reflection high energy electron diffraction, synchrotron hard X-ray diffraction, X-ray photoemission spectroscopy, and magnetometry. The surface reconstructions, magnetic properties, crystalline quality, and strain relaxation behavior of the MnSb films are similar to those of MnSb grown on GaAs(111). In contrast to GaAs substrates, segregation of substrate atoms through the MnSb film does not occur, and alternative polymorphs of MnSb are absent

Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair.

Repair of double strand DNA breaks (DSBs) can result in gene disruption or gene modification via homology directed repair (HDR) from donor DNA. Altering cellular responses to DSBs may rebalance editing outcomes towards HDR and away from other repair outcomes. Here, we utilize a pooled CRISPR screen to define host cell involvement in HDR between a Cas9 DSB and a plasmid double stranded donor DNA (dsDonor). We find that the Fanconi Anemia (FA) pathway is required for dsDonor HDR and that other genes act to repress HDR. Small molecule inhibition of one of these repressors, CDC7, by XL413 and other inhibitors increases the efficiency of HDR by up to 3.5 fold in many contexts, including primary T cells. XL413 stimulates HDR during a reversible slowing of S-phase that is unexplored for Cas9-induced HDR. We anticipate that XL413 and other such rationally developed inhibitors will be useful tools for gene modification