IMMUNOLOGIC MARKER OF INTRANATAL INFECTIONS

The results of the research established determination of immunologic marker for prognosis of intranatal infection. The authors performed a comparative study of the immunity values of women in a risk group for prenatal infection. As it was shown when prenatal infection develops, there is an increase in the content of proinflammatory cytokines in the pregnant woman serum and activation of the ingestion rate. Account the results to devise an algorithm the pregnant woman care

A clinical case of congenital cytomegalovirus infection in a premature baby. Observation in the catamnesis

The purpose of the study is to demonstrate the importance of a multidisciplinary approach in the timely diagnosis and treatment of a child with congenital cytomegalovirus infection by the example of a clinical case.Цель исследования - на примере клинического случая продемонстрировать важность мультидисциплинарного подхода в своевременной диагностики и лечении ребенка с врожденной цитомегаловирусной инфекцией

Metal Hydrides Form Halogen Bonds: Measurement of Energetics of Binding

The formation of halogen bonds from iodopentafluorobenzene and 1-iodoperfluorohexane to a series of bis(η5-cyclopentadienyl)metal hydrides (Cp2TaH3, 1; Cp2MH2, M = Mo, 2, M = W, 3; Cp2ReH, 4; Cp2Ta(H)CO, 5; Cp = η5-cyclopentadienyl) is demonstrated by 1H NMR spectroscopy. Interaction enthalpies and entropies for complex 1 with C6F5I and C6F13I are reported (ΔH° = −10.9 ± 0.4 and −11.8 ± 0.3 kJ/mol; ΔS° = −38 ± 2 and −34 ± 2 J/(mol·K), respectively) and found to be stronger than those for 1 with the hydrogen-bond donor indole (ΔH° = −7.3 ± 0.1 kJ/mol, ΔS° = −24 ± 1 J/(mol·K)). For the more reactive complexes 2–5, measurements are limited to determination of their low-temperature (212 K) association constants with C6F5I as 2.9 ± 0.2, 2.5 ± 0.1, <1.5, and 12.5 ± 0.3 M–1, respectively

The Magnitude and Mechanism of Charge Enhancement of CH∙∙O H-bonds

Quantum calculations find that neutral methylamines and thioethers form complexes, with N-methylacetamide (NMA) as proton acceptor, with binding energies of 2–5 kcal/mol. This interaction is magnified by a factor of 4–9, bringing the binding energy up to as much as 20 kcal/mol, when a CH3+ group is added to the proton donor. Complexes prefer trifurcated arrangements, wherein three separate methyl groups donate a proton to the O acceptor. Binding energies lessen when the systems are immersed in solvents of increasing polarity, but the ionic complexes retain their favored status even in water. The binding energy is reduced when the methyl groups are replaced by longer alkyl chains. The proton acceptor prefers to associate with those CH groups that are as close as possible to the S/N center of the formal positive charge. A single linear CH··O hydrogen bond (H-bond) is less favorable than is trifurcation with three separate methyl groups. A trifurcated arrangement with three H atoms of the same methyl group is even less favorable. Various means of analysis, including NBO, SAPT, NMR, and electron density shifts, all identify the +CH··O interaction as a true H-bond

EPIDEMIOLOGIC CHARACTERISTICS AND IMMUNOLOGICAL ASPECTS OF PRENATAL INFECTION

Abstract. To improve diagnostics of prenatal infections the basic obstetrics indices in Omsk region in the period of 2000–2010 years have been analyzed. It was found that perinatal mortality reduced 2.5 times (from 14.5 till 5.7 per 1000 newborns) as well as neonatal mortality (from 6.5 till 0.9 per 1000 newborns). The laboratory testing of 187 newborns (85 pairs mother-newborn) revealed monoinfection in 24% of cases and mixed infection in 7% of cases with predomination of herpes viruses (HHV-6, CMV, EBV, HSV1,2). Newborns with manifested infections had increased number of cytotoxic T-cells, intensive expression of HLA-DR antigens on monocytes and IL-8 chemokine increased production

IMMUNOPHENOTYPING OF BLOOD LYMPHOCYTES IN NEWBORNS WITH PRENATAL INFECTIONS AT DIFFERENT GESTATION TERMS

The study aimed to compare subset composition of peripheral blood lymphocytes in 40 newborns at different gestation periods, complicated by a verified prenatal infection (1st group), or with hypoxic brain damage (2nd group), using a flow cytometry technique. It was found that activated CD14+HLA-DR+ monocytes were 2,3-fold more common in the infants from the 1st group, and representation of CD5+CD19+ B1 lymphocytes (a «forbidden» clone) was 1,8-fold higher than in 2nd group. Among pre-term babies from the 1st group, the lymphocytes with CD3+CD16+/CD56+, CD3+CD95+ and CD5+CD19+ phenotype were registered more often, than in full-term infants (resp., 5, 11, and 2 times higher). These data argue for involvement of compensatory defense immune mechanisms under the conditions of increased antigenic load, being more pronounced in pre-term infants

SURVIVAL OF BACTERIAL CULTURES AND VARIATION OF BIOCHEMICAL PROPERTIES IN THE LONG-TERM STORAGE

We analyzed 67 strains from the collection of psychrophilic bacteria isolated from the water and bottom sediments of Lake Baikal in 2002 and 14 strains of mesophilic heterotrophic bacteria isolated from the digestive system of Baikal gastropods in 2010–2011. Biochemical properties were studied for 26 strains. We performed a comparative analysis of the enzymatic activities of the strains isolated in 2002 and their re-isolates of the 2014.Исследование выполнено при финансовой поддержке Российского фонда фундаментальных исследований в рамках научного проекта № 14-04-14-04-90416-Укр а