Microtubule rescue at midzone edges promotes overlap stability and prevents spindle collapse during anaphase B

During anaphase B, molecular motors slide interpolar microtubules to elongate the mitotic spindle, contributing to the separation of chromosomes. However, sliding of antiparallel microtubules reduces their overlap, which may lead to spindle breakage, unless microtubules grow to compensate sliding. How sliding and growth are coordinated is still poorly understood. In this study, we have used the 1ssion yeast S. pombe to measure microtubule dynamics during anaphase B. We report that the coordination of microtubule growth and sliding relies on promoting rescues at the midzone edges. This makes microtubules stable from pole to midzone, while their distal parts including the plus ends alternate between assembly and disassembly. Consequently, the midzone keeps a constant length throughout anaphase, enabling sustained sliding without the need for a precise regulation of microtubule growth speed. Additionally, we found that in S. pombe, which undergoes closed mitosis, microtubule growth speed decreases when the nuclear 22 membrane wraps around the spindle midzone

Microtubule rescue at midzone edges promotes overlap stability and prevents spindle collapse during anaphase B

During anaphase B, molecular motors slide interpolar microtubules to elongate the mitotic spindle, contributing to the separation of chromosomes. However, sliding of antiparallel microtubules reduces their overlap, which may lead to spindle breakage, unless microtubules grow to compensate sliding. How sliding and growth are coordinated is still poorly understood. In this study, we have used the fission yeastS. pombeto measure microtubule dynamics during anaphase B. We report that the coordination of microtubule growth and sliding relies on promoting rescues at the midzone edges. This makes microtubules stable from pole to midzone, while their distal parts including the plus ends alternate between assembly and disassembly. Consequently, the midzone keeps a constant length throughout anaphase, enabling sustained sliding without the need for a precise regulation of microtubule growth speed. Additionally, we found that inS. pombe, which undergoes closed mitosis, microtubule growth speed decreases when the nuclear membrane wraps around the spindle midzone

An alternative approach for robot localization inside pipes using RF spatial fadings

Accurate robot localization represents a challenge inside pipes due to the particular conditions that characterize this type of environment. Outdoor techniques (GPS in particular) do not work at all inside metal pipes, while traditional indoor localization methods based on camera or laser sensors do not perform well mainly due to a lack of external illumination and distinctive features along pipes. Moreover, humidity and slippery surfaces make wheel odometry unreliable. In this paper, we estimate the localization of a robot along a pipe with an alternative Radio Frequency (RF) approach. We first analyze wireless propagation in metallic pipes and propose a series of setups that allow us to obtain periodic RF spatial fadings (a sort of standing wave periodic pattern), together with the influence of the antenna position and orientation over these fadings. Subsequently, we propose a discrete RF odometry-like method, by means of counting the fadings while traversing them. The transversal fading analysis (number of antennas and cross-section position) makes it possible to increase the resolution of this method. Lastly, the model of the signal is used in a continuous approach serving as an RF map. The proposed localization methods outperform our previous contributions in terms of resolution, accuracy, reliability and robustness. Experimental results demonstrate the effectiveness of the RF-based strategy without the need for a previously known map of the scenario or any substantial modification of the existing infrastructure

The demand for sports and exercise: Results from an illustrative survey

Funding from the Department of Health policy research programme was used in this study.There is a paucity of empirical evidence on the extent to which price and perceived benefits affect the level of participation in sports and exercise. Using an illustrative sample of 60 adults at Brunel University, West London, we investigate the determinants of demand for sports and exercise. The data were collected through face-to-face interviews that covered indicators of sports and exercise behaviour; money/time price and perceived benefits of participation; and socio- economic/demographic details. Count, linear and probit regression models were fitted as appropriate. Seventy eight per cent of the sample participated in sports and exercise and spent an average of £27 per month and an average of 20 min travelling per occasion of sports and exercise. The demand for sport and exercise was negatively associated with time (travel or access time) and ‘variable’ price and positively correlated with ‘fixed’ price. Demand was price inelastic, except in the case of meeting the UK government’s recommended level of participation, which is time price elastic (elasticity = −2.2). The implications of data from a larger nationally representative sample as well as the role of economic incentives in influencing uptake of sports and exercise are discussed.This article is available through the Brunel Open Access Publishing Fund

Joint analysis constraints on the physics of the first galaxies with low frequency radio astronomy data

Observations of the first billion years of cosmic history are currently limited. We demonstrate, using a novel machine learning technique, the synergy between observations of the sky-averaged 21-cm signal from neutral hydrogen and interferometric measurements of the corresponding spatial fluctuations. By jointly analysing data from SARAS3 (redshift $z\approx15-25$) and limits from HERA ($z\approx8$ and $10$), we show that such a synergetic analysis provides tighter constraints on the astrophysics of galaxies 200 million years after the Big Bang than can be achieved with the individual data sets. Although our constraints are weak, this is the first time data from a sky-averaged 21-cm experiment and power spectrum experiment have been analysed together. In synergy, the two experiments leave only $64.9^{+0.3}_{-0.1}$ % of the explored broad theoretical parameter space to be consistent with the joint data set, in comparison to $92.3^{+0.3}_{-0.1}$ % for SARAS3 and $79.0^{+0.5}_{-0.2}$ % for HERA alone. We use the joint analysis to constrain star formation efficiency, minimum halo mass for star formation, X-ray luminosity of early emitters and the radio luminosity of early galaxies. The joint analysis disfavours at 68 % confidence a combination of galaxies with X-ray emission that is $\lesssim 33$ and radio emission that is $\gtrsim 32$ times as efficient as present day galaxies. We disfavour at 95 % confidence scenarios in which power spectra are $\geq126$ mK$^{2}$ at $z=25$ and the sky-averaged signals are $\leq-277$ mK.Comment: Submitte

Differences in formal and informal sports participation at regional level in England

Purpose: The aim of the paper is to provide a regional approach to analyse sports participation in two different contexts: organised/formal versus non-organised/informal participation, using the England's Active People Survey (APS) national dataset. Method: We have estimated two models: first, a general model to explain differences in regional informal and formal participation rates; second, an econometric model dealing with formal participation at a regular frequency. Results: The results emphasise the different roles played by some correlates depending on the context of sports participation under study. Only economic and cultural variables seem to have a general influence throughout all the sports participation contexts. The results reinforce the role played by sport supply and sport funding in some sports participation levels, offering interesting implications for sport policy. The urban environment, for example, appears to be positively related to the transition from informal to formal sport participation. Conclusions: The distinct analysis of the sports participation contexts provides the opportunity to evaluate ways of boosting that participation as well as to suggest some interesting policy implications towards this aim. For example, sporting infrastructure is only influential for the transition from non-participation to formal participation, implying that in general the key question about sport funding and supply is not the amount of funds but rather the direction and aims of sport policy. Finally, the paper offers some explanations about the gender inequality detected in some forms of sports participation. Keywords: sports participation, formal and informal participation, sports infrastructure, Dirichlet model

The FXII c.-4T > C Polymorphism as a Disease Modifier in Patients With Hereditary Angioedema Due to the FXII p.Thr328Lys Variant

Background: Hereditary angioedema due to the Thr328Lys variant in the coagulation factor XII (HAE-FXII) affects mainly women in whom the symptomatology is dependent on high estrogen levels. Clinical variability and incomplete penetrance are challenging features that hinder the diagnosis and management of HAE-FXII. The c.-4T>C Kozak polymorphism is the only common variation accounting for FXII plasma levels and was previously shown to modify the course of HAE due to C1-Inhibitor deficiency. Objectives: To assess the influence of the c.-4T>C polymorphism on disease expression in 39 Spanish HAE-FXII index patients. Methods: The c.-4T>C polymorphism was sequenced by the standard Sanger method, and HAE severity was calculated according to the score by Cumming et al. (2003) The activation of the contact system was quantified by the kallikrein-like activity of plasma in chromogenic assays upon activation with high-molecular-weight dextran sulfate. Results: The c.-4CC genotype was overrepresented in the studied cohort: 82% were CC-homozygous (expected frequency = 59%) and 18% were CT-heterozygous (expected frequency = 39%) (p = 0.001). Patients with a c.-4CC genotype exhibited higher kallikrein-like activity (0.9659 +/- 0.1136) than those with a c.-4TC genotype (0.7645 +/- 0.1235) (p = 0.024) or healthy donors. Moreover, the polymorphism influenced HAE-FXII severity score (c.-4CC = 4.43 +/- 2.28 vs c.-4TC = 2.0 +/- 1.15; p = 0.006) but not the degree of estrogen dependence or time until remission. Conclusion: The c.-4T>C polymorphism is overrepresented in a Spanish HAE-FXII cohort and significantly influences the degree of contact system activation and the clinical severity of the disease

Rethinking ecosystem service indicators for their application to intermittent rivers

In these times of strong pressure on aquatic ecosystems and water resources due to climate change and water abstraction, intermittent rivers and ephemeral streams (IRES) (rivers that periodically cease to flow and/or dry) have become valuable assets. Indeed, not only do they supply water but they also offer services for humanity. Despite a growing recognition towards IRES, information for assessing their ecosystem services (ES) remains scarce. In a first step, an international interdisciplinary group of researchers developed a methodological framework to acknowledge ES provided by IRES using 109 indicators. A subset of selected ES indicators was then applied to two case studies: the Rio Seco in the Algarve (Portugal) and the Giofyros River in Crete (Greece). This paper discusses the applicability of these indicators, including the temporal and spatial variability of IRES flow regimes. Aspects of the framework, such as the methods and time required for data collection, the nature (demand or supply) and functionality of each indicator are discussed. The new framework accounts for flow intermittence in ES analyses and can help scientists and water managers to i) increase the ease and justification for IRES use in management approaches and ii) improve their conservation and restoration with a comprehensive set of appropriate indicators for IRES. In addition, the comprehensive nature of the proposed indicators ensures that they can be understood by a broad audience and easily applicable. Since they were designed through a public participation process, the setting has been prepared for holistic stakeholder analysis and education around IRES functions and associated ES. From a management point of view, it would be particularly relevant to perform an economic evaluation with this new framework to understand the value of each ES category and their trade-offs. For the scientific community, however, it is important to consider public preferences to design socially accepted policies. The proposed indicators can successfully bridge these elements, hereby establishing a solid basis for the assessment of ES provided by IRES.info:eu-repo/semantics/publishedVersio