Old case, new leads: miRNA links Kaposi’s sarcoma-associated herpesvirus with sepsis

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Pasture Yield and Animal Performance from Aeschynomene Americana Cultivars Glenn and Lee

An on farm demonstration site was used to promote and compare the performance of the tropical legumes Glenn American jointvetch and Lee American jointvetch (Aeschynomene americana). Both legumes are widely sown in tropical Australia and are being increasingly sown in South-East Asian pasture projects. Glenn and Lee both grew well and persisted in grass/legume pastures over a 5 year period. Liveweight gain of steers, at a stocking rate of 1.5/ha, slightly favoured the Glenn pasture and gains of 0.49 kg/day for a 595 day period were achieved. Soil seed reserves were much higher under the annual Glenn than for the later flowering perennial Lee. Current recommendations are to sow equal portions of Glenn and Lee in legume/grass pasture mixture

Managing for rainfall variability: effect of grazing strategy on cattle production in a dry tropical savanna

Rainfall variability is a challenge to sustainable and pro. table cattle production in northern Australia. Strategies recommended to manage for rainfall variability, like light or variable stocking, are not widely adopted. This is due partly to the perception that sustainability and profitability are incompatible. A large, long-term grazing trial was initiated in 1997 in north Queensland, Australia, to test the effect of different grazing strategies on cattle production. These strategies are: (i) constant light stocking (LSR) at long-term carrying capacity (LTCC); (ii) constant heavy stocking (HSR) at twice LTCC; (iii) rotational wet-season spelling (R/Spell) at 1.5 LTCC; (iv) variable stocking (VAR), with stocking rates adjusted in May based on available pasture; and (v) a Southern Oscillation Index (SOI) variable strategy, with stocking rates adjusted in November, based on available pasture and SOI seasonal forecasts. Animal performance varied markedly over the 10 years for which data is presented, due to pronounced differences in rainfall and pasture availability. Nonetheless, lighter stocking at or about LTCC consistently gave the best individual liveweight gain (LWG), condition score and skeletal growth; mean LWG per annum was thus highest in the LSR (113 kg), intermediate in the R/Spell (104 kg) and lowest in the HSR(86 kg). MeanLWGwas 106 kg in the VAR and 103 kg in the SOI but, in all years, the relative performance of these strategies was dependent upon the stocking rate applied. After 2 years on the trial, steers from lightly stocked strategies were 60-100 kg heavier and received appreciable carcass price premiums at the meatworks compared to those under heavy stocking. In contrast, LWG per unit area was greatest at stocking rates of about twice LTCC; mean LWG/ha was thus greatest in the HSR (21 kg/ha), but this strategy required drought feeding in four of the 10 years and was unsustainable. Although LWG/ha was lower in the LSR (mean 14 kg/ha), or in strategies that reduced stocking rates in dry years like the VAR(mean 18 kg/ha) and SOI (mean 17 kg/ha), these strategies did not require drought feeding and appeared sustainable. The R/Spell strategy (mean 16 kg/ha) was compromised by an ill-timed fire, but also performed satisfactorily. The present results provide important evidence challenging the assumption that sustainable management in a variable environment is unprofitable. Further research is required to fully quantify the long-term effects of these strategies on land condition and profitability and to extrapolate the results to breeder performance at the property level

More Aeschynomene Pasture Legumes for the Tropics and Sub-Tropics

Two cultivars of Aeschynomene villosa were released in Queensland in 1995. Cultivars ‘Reid’ (previously CPI 91209) and ‘Kretschmer’ (previously CPI 93621) are both native to Mexico and are the first cultivars of A. villosa to be released as pasture legumes. They will complement the three Aeschynomene cultivars already in use in tropical and sub-tropical northern and eastern Australia, namely cultivars ‘Glenn’ and ‘Lee’ (A. americana) and ‘Bargoo’ (A. falcata). Characteristics of Reid and Kretschmer jointvetch include, early seeding perennials within high seed yields, prostrate to semi-erect growth habit, adaptation to a wide range of soil types in medium rainfall areas, tolerance of temporary waterlogging, palatability of high quality forage, responsive to added phosphorus, and tolerance of light frosts. Commercial seed will be available for sale about October, 1997. Reid and Kretschmer villose jointvetch are widely adapted high quality pasture legumes which will complement current legume cultivars and will broaden the scope for sown pasture development in sub-tropical and tropical northern Australia

A 340/380 nm light emitting diode illuminator for Fura-2 AM ratiometric Ca2+ imaging of live cells with better than 5 nM precision

We report the first demonstration of a fast wavelength-switchable 340/380 nm light emitting diode (LED) illuminator for Fura-2 ratiometric Ca2+ imaging of live cells. The LEDs closely match the excitation peaks of bound and free Fura-2 and enables the precise detection of cytosolic Ca2+ concentrations, which is only limited by the Ca2+ response of Fura-2. Using this illuminator, we have shown that Fura-2 acetoxymethyl ester (AM) concentrations as low as 250 nM can be used to detect induced Ca2+ events in tsA-201 cells and while utilizing the 150 µs switching speeds available, it was possible to image spontaneous Ca2+ transients in hippocampal neurons at a rate of 24.39 Hz that were blunted or absent at typical 0.5 Hz acquisition rates. Overall, the sensitivity and acquisition speeds available using this LED illuminator significantly improves the temporal resolution that can be obtained in comparison to current systems and supports optical imaging of fast Ca2+ events using Fura-2

Using remote sensing to forecast forage quality for cattle in the dry savannas of northeast Australia

In the dry savannas of northeast Australia, forage quality is just as important for cattle production as forage quantity. The seasonal trend of forage quality is broadly predictable by land managers, but it is more difficult to predict the point when quality—which depends on local climate, management, and pasture condition—falls below the requirement for animal maintenance. In this study we use statistical modelling to forecast how forage quality might change at the crucial time of year, i.e., as the summer wet season transitions to the dry winter. We do this with the aid of historical information associated with a long-term cattle-grazing trial in the dry savannas. We combined multiple years of two measures of forage quality (dietary crude protein and in vivo dry-matter digestibility; respectively DCP and DMD) and ground cover information (specifically the ratio of ‘green grass’ cover to ‘dead (i.e., non-photosynthetic) grass’ cover, derived from an archive of Landsat satellite imagery) into a linear mixed model that explicitly considered the correlations with time and between variables. DCP and DMD were estimated by near-infrared spectroscopy of fresh faecal samples; values did not have to be temporally coincident with the satellite imagery. With the end of May considered a nominal decision-point, we forecast monthly averages of forage quality for June to August, over a 12-year period at the study site. Over all months and all years, the median absolute error of the forecasts was DCP = 0.86%, and DMD = 0.95%. The remote sensing information served as a correlated, oft-sampled covariate that helped to guide the forecasts of forage quality. We propose summarising the forecasts (and their uncertainty) as a near-real-time graphical tool for decision-support. Such a product could potentially benefit cattle-grazing enterprises in the northeast of Australia, enabling more timely management of herds through the dry season

Remodelling of a polypyrimidine tract-binding protein complex during apoptosis activates cellular IRESs.

Post-transcriptional control of gene expression is mediated by the interaction of RNA-binding proteins with their cognate mRNAs that specifically regulate their stability, localization and translation. mRNA-binding proteins are multifunctional and it has been proposed therefore that a combinatorial RNA-binding protein code exists that allows specific protein sub-complexes to control cytoplasmic gene expression under a range of pathophysiological conditions. We show that polypyrimidine tract-binding protein (PTB) is central to one such complex that forms in apoptotic cells. Thus, during apoptosis initiated by TNF-related apoptosis inducing ligand there is a change in the repertoire of RNA-binding proteins with which PTB interacts. We show that altering the cellular levels of PTB and its binding partners, either singly or in combination, is sufficient to directly change the rates of apoptosis with increased expression of PTB, YBX1, PSF and NONO/p54(nrb) accelerating this process. Mechanistically, we show that these proteins post-transcriptionally regulate gene expression, and therefore apoptotic rates, by interacting with and stimulating the activity of RNA elements (internal ribosome entry segments) found in mRNAs that are translated during apoptosis. Taken together, our data show that PTB function is controlled by a set of co-recruited proteins and importantly provide further evidence that it is possible to dictate cell fate by modulating cytoplasmic gene expression pathways alone

correction novel chemical probes for the investigation of nonribosomal peptide assembly

Correction for 'Novel chemical probes for the investigation of nonribosomal peptide assembly' by Y. T. Candace Ho et al., Chem. Commun., 2017, 53, 7088–7091