1,177 research outputs found
Ion-channel-like behavior in lipid bilayer membranes at the melting transition
It is well known that at the gel-liquid phase transition temperature a lipid
bilayer membrane exhibits an increased ion permeability. We analyze the
quantized currents in which the increased permeability presents itself. The
open time histogram shows a "-3/2" power law which implies an open-closed
transition rate that decreases like as time evolves. We
propose a "pore freezing" model to explain the observations. We discuss how
this model also leads to the noise that is commonly observed in
currents across biological and artificial membranes.Comment: 5 pages, 4 figure
Structural Kinetic Modeling of Metabolic Networks
To develop and investigate detailed mathematical models of cellular metabolic
processes is one of the primary challenges in systems biology. However, despite
considerable advance in the topological analysis of metabolic networks,
explicit kinetic modeling based on differential equations is still often
severely hampered by inadequate knowledge of the enzyme-kinetic rate laws and
their associated parameter values. Here we propose a method that aims to give a
detailed and quantitative account of the dynamical capabilities of metabolic
systems, without requiring any explicit information about the particular
functional form of the rate equations. Our approach is based on constructing a
local linear model at each point in parameter space, such that each element of
the model is either directly experimentally accessible, or amenable to a
straightforward biochemical interpretation. This ensemble of local linear
models, encompassing all possible explicit kinetic models, then allows for a
systematic statistical exploration of the comprehensive parameter space. The
method is applied to two paradigmatic examples: The glycolytic pathway of yeast
and a realistic-scale representation of the photosynthetic Calvin cycle.Comment: 14 pages, 8 figures (color
Three and four current reversals versus temperature in correlation ratchets with a simple sawtooh potential
Transport of Brownian particles on a simple sawtooth potential subjected to
both unbiased thermal and nonequilibrium symmetric three-level Markovian noise
is considered. The new effects of three and four current reversals as a
function of temperature are established in such correlation ratchets. The
parameter space coordinates of the fixed points associated with these current
reversals and the necessary and sufficient conditions for the existence of the
novel current reversals are found.Comment: 4 pages, 5 figures; some changes introduced; accepted for publication
in Physical Review
Identificação por espectrometria de massa MALDI-TOF de Salmonella spp. e Escherichia coli isolados de carcaças bovinas.
The aim of this study was to introduce matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry to improve the traditional microbiological method for the detection of Salmonella spp. and Escherichia coli in beef carcasses. Two hundred seventy samples from 90 beef carcasses were evaluated. The methodologies described in ISO 6579:2002 and in the Compendium of Methods for the Microbiological Examination of Foods were used for Salmonella spp. and E. coli isolation, respectively. MALDI-TOF analysis were performed on tryptone soya broth suspension isolates or directly from nutrient agar colonies, from the positive, inconclusive or negative biochemically tested samples for Salmonella and E. coli. Mass profiles were acquired on an Autoflex III SmartBeam MALDI-TOF mass spectrometer and the raw spectra were processed using the MALDI Biotyper software (Bruker Daltonics). According to the preliminary identification based on colony morphology and the biochemical reactions, seven isolates were positive for Salmonella spp. Through MALDI Biotyper these seven isolates were also classified as belonging to the genus Salmonella and further identified as S. enterica. Four isolates showing unusual phenotypic characteristics and inconclusive results in biochemical tests for Salmonella were identified as belonging to Citrobacter and Proteus genera after MALDI analysis. Regarding Escherichia coli, 37 were positive for species biochemical testing which MALDI Biotyper confirmed. MALDI-TOF methodology allowed rapid Salmonella spp. and E. coli identity confirmation and may be used to detect these microrganisms within bacterial isolates from beef carcasses.Título em inglês: MALDI-TOF mass spectrometry identification of Salmonella spp. and Escherichia coli isolated from bovine carcasses
Acupuncture for Treatment of Autism Spectrum Disorders
Background. There has been lack of reviews of evidence on efficacy, methodology, and/or safety of acupuncture in autism spectrum disorders. This paper examines the emerging evidence of the effects of acupuncture in the treatment of autistic children. Method. A literature review was completed via Medline and three Chinese search engines. A total of 31 studies were evaluated for acupuncture methodology, study design, treatment effects, and tolerability. Results. The acupoints used, the duration of needling, the frequency of treatment, the choice of stimulation, and the course of the treatment were highly variable amongst the studies. Behavioral and/or developmental improvements were reported in all acupuncture treatment studies. All studies reported general tolerability. Weakness of experimental designs was discussed. Conclusions. Vigorously controlled double-blinded clinical trials are needed to evaluate the efficacy and safety of acupuncture in children with autism spectrum disorders
Separation quality of a geometric ratchet
We consider an experimentally relevant model of a geometric ratchet in which
particles undergo drift and diffusive motion in a two-dimensional periodic
array of obstacles, and which is used for the continuous separation of
particles subject to different forces. The macroscopic drift velocity and
diffusion tensor are calculated by a Monte-Carlo simulation and by a
master-equation approach, using the correponding microscopic quantities and the
shape of the obstacles as input. We define a measure of separation quality and
investigate its dependence on the applied force and the shape of the obstacles
Active Brownian Motion Models and Applications to Ratchets
We give an overview over recent studies on the model of Active Brownian
Motion (ABM) coupled to reservoirs providing free energy which may be converted
into kinetic energy of motion. First, we present an introduction to a general
concept of active Brownian particles which are capable to take up energy from
the source and transform part of it in order to perform various activities. In
the second part of our presentation we consider applications of ABM to ratchet
systems with different forms of differentiable potentials. Both analytical and
numerical evaluations are discussed for three cases of sinusoidal,
staircase-like and Mateos ratchet potentials, also with the additional loads
modeled by tilted potential structure. In addition, stochastic character of the
kinetics is investigated by considering perturbation by Gaussian white noise
which is shown to be responsible for driving the directionality of the
asymptotic flux in the ratchet. This \textit{stochastically driven
directionality} effect is visualized as a strong nonmonotonic dependence of the
statistics of the right versus left trajectories of motion leading to a net
current of particles. Possible applications of the ratchet systems to molecular
motors are also briefly discussedComment: 12 pages, 17 figure
Exposure to Leptospira spp. and associated risk factors in the human, cattle and dog populations in Bhutan
Leptospirosis is a neglected worldwide zoonotic bacterial disease with a high prevalence in subtropical and tropical countries. The prevalence of Leptospira spp. in humans, cattle and dogs is unknown in Bhutan. Therefore, we sought to find out whether humans, cattle or dogs had been infected in the past with leptospires by measuring antibodies in the serum. We therefore collected blood from 864 humans >/=13 years of age, 130 bovines and 84 dogs from different rural and urban areas in Bhutan and tested the serum for antibodies specific for leptospires with a screening of enzyme-linked immunosorbent assays (ELISA) and a confirmatory microscopic agglutination test (MAT). In humans, 17.6% were seropositive by ELISA and 1.6% by MAT. The seropositivity was stronger in bovines (36.9%) and dogs (47.6%). "Having had a fever recently" (OR 5.2, p = 0.004), "working for the military" (OR 26.6, p = 0.028) and "being unemployed" (OR 12.9, p = 0.041) (reference category = housemaker) were statistically significantly associated with seropositivity when controlled for the effects of other risk factors. However, due to the small number of positive test results, the findings on risk factors should be interpreted with caution. Based on the serogroups found in the three species, dogs could be a source of infection for humans, or dogs and humans are exposed to the same environmental risk factors Clinical leptospirosis in humans and domestic animals should be investigated by testing blood and urine for the presence of leptospires by molecular methods (qPCR)
- …