298 research outputs found
Wolbachia endosymbiont of the horn fly Haematobia irritans irritans: a supergroup A strain with multiple horizontally acquired cytoplasmic incompatibility genes
The horn fly, Haematobia irritans irritans, is a hematophagous parasite of livestock distributed throughout Europe, Africa, Asia, and the Americas. Welfare losses on livestock due to horn fly infestation are estimated to cost between USD 1-2.5 billion annually in North America and Brazil. The endosymbiotic bacterium Wolbachia pipientis is a maternally inherited manipulator of reproductive biology in arthropods and naturally infects laboratory colonies of horn flies from Kerrville, USA and Alberta, Canada, but has also been identified in wild-caught samples from Canada, USA, Mexico and Hungary. Re-assembly of PacBio long-read and Illumina genomic DNA libraries from the Kerrville H. i. irritans genome project allowed for a complete and circularised 1.3 Mb Wolbachia genome (wIrr). Annotation of wIrr yielded 1249 coding genes, 34 tRNAs, three rRNAs, and five prophage regions. Comparative genomics and whole genome Bayesian evolutionary analysis of wIrr compared to published Wolbachia genomes suggests that wIrr is most closely related to and diverged from Wolbachia supergroup A strains known to infect Drosophila spp. Whole-genome synteny analyses between wIrr and closely related genomes indicates that wIrr has undergone significant genome rearrangements while maintaining high nucleotide identity. Comparative analysis of the cytoplasmic incompatibility (CI) genes of wIrr suggests two phylogenetically distinct CI loci and acquisition of another CifB homolog from phylogenetically distant supergroup A Wolbachia strains suggesting horizontal acquisition of these loci. The wIrr genome provides a resource for future examination of the impact Wolbachia may have in both biocontrol and potential insecticide resistance of horn flies
Gravitational Collapse of Inhomogeneous Dust in (2+1) Dimensions
We examine the gravitational collapse of spherically symmetric inhomogeneous
dust in (2+1) dimensions, with cosmological constant. We obtain the analytical
expressions for the interior metric. We match the solution to a vacuum
exterior. We discuss the nature of the singularity formed by analyzing the
outgoing radial null geodesics. We examine the formation of trapped surfaces
during the collapse.Comment: Accepted for publication in CQ
Collapse Dynamics of a Star of Dark Matter and Dark Energy
In this work, we study the collapse dynamics of an inhomogeneous spherically
symmetric star made of dark matter (DM) and dark energy (DE). The dark matter
is taken in the form of a dust cloud while anisotropic fluid is chosen as the
candidate for dark energy. It is investigated how dark energy modifies the
collapsing process and is examined whether dark energy has any effect on the
Cosmic Censorship Conjecture. The collapsing star is assumed to be of finite
radius and the space time is divided into three distinct regions and
, where represents the boundary of the star and
denotes the interior (exterior) of the star. The junction
conditions for matching over are specified. Role of Dark
energy in the formation of apparent horizon is studied and central singularity
is analyzed.Comment: 13 page
Instruments of RT-2 Experiment onboard CORONAS-PHOTON and their test and evaluation V: Onboard software, Data Structure, Telemetry and Telecommand
The onboard software and data communication in the RT-2 Experiment onboard
the Coronas-Photon satellite is organized in a hierarchical way to effectively
handle and communicate asynchronous data generated by the X-ray detectors. A
flexible data handling system is organized in the X-ray detector packages
themselves and the processing electronic device, namely RT-2/E, has the
necessary intelligence to communicate with the 3 scientific payloads by issuing
commands and receiving data. It has direct interfacing with the Satellite
systems and issues commands to the detectors and processes the detector data
before sending to the satellite systems. The onboard software is configured
with several novel features like a) device independent communication scheme, b)
loss-less data compression and c) Digital Signal Processor. Functionality of
the onboard software along with the data structure, command structure, complex
processing scheme etc. are discussed in this paper.Comment: 24 pages, 2 figures, Accepted for publication in Experimental
Astronomy (in press
Gravitational collapse in asymptotically Anti-de Sitter/de Sitter backgrounds
We study here the gravitational collapse of a matter cloud with a
non-vanishing tangential pressure in the presence of a non-zero cosmological
term. Conditions for bounce and singularity formation are derived for the
model. It is also shown that when the tangential pressures vanish, the bounce
and singularity conditions reduce to that of the dust case studied earlier. The
collapsing interior is matched with an exterior which is asymptotically de
Sitter or anti de Sitter, depending on the sign of cosmological constant. The
junction conditions for matching the cloud to exterior are specified. The
effect of the cosmological term on apparent horizons is studied in some detail,
and the nature of central singularity is analyzed. We also discuss here the
visibility of the singularity and implications for the cosmic censorship
conjecture.Comment: 11 pages, 1 figure, Revtex
Classical and Quantum Equations of Motion for a BTZ Black String in AdS Space
We investigate gravitational collapse of a -dimensional BTZ black
string in AdS space in the context of both classical and quantum mechanics.
This is done by first deriving the conserved mass per unit length of the
cylindrically symmetric domain wall, which is taken as the classical
Hamiltonian of the black string. In the quantum mechanical context, we take
primary interest in the behavior of the collapse near the horizon and near the
origin (classical singularity) from the point of view of an infalling observer.
In the absence of radiation, quantum effects near the horizon do not change the
classical conclusions for an infalling observer, meaning that the horizon is
not an obstacle for him/her. The most interesting quantum mechanical effect
comes in when investigating near the origin. First, quantum effects are able to
remove the classical singularity at the origin, since the wave function is
non-singular at the origin. Second, the Schr\"odinger equation describing the
behavior near the origin displays non-local effects, which depend on the energy
density of the domain wall. This is manifest in that derivatives of the
wavefunction at one point are related to the value of the wavefunction at some
other distant point.Comment: 9 pages, 1 figure. Minor Clarification and corrections. Accepted for
Publication in JHE
Development of compact bandpass filter using symmetrical metamaterial structures for GPS, ISM, Wi-MAX, and WLAN applications
This article describes the development of a compact microstrip bandpass filter (BPF) for multiple wireless communication utilizations. The proposed bandpass filter consists of metamaterial unit cells that are symmetrical in shape. The design process involves the placement of four symmetrical split-ring resonators (SRRs) on the top plane of the BPF. It exhibits improved filter characteristics through the implementation of these SRRs. The filter was modeled and fabricated and its performance was evaluated using a Vector Network Analyzer. The designed bandpass filter shows a 5 GHz bandwidth covering the frequency band spanning from 1 to 5.2 GHz, with a quality factor value of 1.85 across 1.9 GHz, 3.3 across 3.3 GHz and 5.1 across 5.1 GHz. The metamaterial analysis was carried out using ANSYS ELECTRONIC DESKTOP. The proposed filter measures 20 × 18 × 1.6 mm3, which is significantly smaller than current filters. The designed bandpass filter occupies 50% of the space of a conventional filter. The designed bandpass filter exhibits a distributed surface current of 84 A/m, and 94 A/m across the wide- and narrow-band operating frequency. The simulated and measured results indicate that the suggested metamaterial filter is well-suited for multiband wireless applications like GPS (1.57 GHz), WLAN (2.4, 3.6, and 5.2 GHz), Wi-MAX (2.3, 2.5, and 3.5 GHz), and ISM (2.5 GHz)
Active Tension Network model suggests an exotic mechanical state realized in epithelial tissues.
Mechanical interactions play a crucial role in epithelial morphogenesis, yet understanding the complex mechanisms through which stress and deformation affect cell behavior remains an open problem. Here we formulate and analyze the Active Tension Network (ATN) model, which assumes that the mechanical balance of cells within a tissue is dominated by cortical tension and introduces tension-dependent active remodeling of the cortex. We find that ATNs exhibit unusual mechanical properties. Specifically, an ATN behaves as a fluid at short times, but at long times supports external tension like a solid. Furthermore, an ATN has an extensively degenerate equilibrium mechanical state associated with a discrete conformal - "isogonal" - deformation of cells. The ATN model predicts a constraint on equilibrium cell geometries, which we demonstrate to approximately hold in certain epithelial tissues. We further show that isogonal modes are observed in the fruit y embryo, accounting for the striking variability of apical areas of ventral cells and helping understand the early phase of gastrulation. Living matter realizes new and exotic mechanical states, the study of which helps to understand biological phenomena
Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2)
We demonstrate the first application of synthetic RNA gene silencers in Streptomyces coelicolor A3(2). Peptide nucleic acid and expressed antisense RNA silencers successfully inhibited actinorhodin production. Synthetic RNA silencing was target-specific and is a new tool for gene regulation and metabolic engineering studies in Streptomyces.Peer reviewe
One vaccine to counter many diseases? Modeling the economics of oral polio vaccine against child mortality and COVID-19
INTRODUCTION: Recent reviews summarize evidence that some vaccines have heterologous or non-specific effects (NSE), potentially offering protection against multiple pathogens. Numerous economic evaluations examine vaccines\u27 pathogen-specific effects, but less than a handful focus on NSE. This paper addresses that gap by reporting economic evaluations of the NSE of oral polio vaccine (OPV) against under-five mortality and COVID-19.
MATERIALS AND METHODS: We studied two settings: (1) reducing child mortality in a high-mortality setting (Guinea-Bissau) and (2) preventing COVID-19 in India. In the former, the intervention involves three annual campaigns in which children receive OPV incremental to routine immunization. In the latter, a susceptible-exposed-infectious-recovered model was developed to estimate the population benefits of two scenarios, in which OPV would be co-administered alongside COVID-19 vaccines. Incremental cost-effectiveness and benefit-cost ratios were modeled for ranges of intervention effectiveness estimates to supplement the headline numbers and account for heterogeneity and uncertainty.
RESULTS: For child mortality, headline cost-effectiveness was 23,000-65,000 if it were administered simultaneously with a COVID-19 vaccine \u3c200 days into a wave of the epidemic. If the COVID-19 vaccine availability were delayed, the cost per averted death would decrease to $2600-6100. Estimated benefit-to-cost ratios vary but are consistently high.
DISCUSSION: Economic evaluation suggests the potential of OPV to efficiently reduce child mortality in high mortality environments. Likewise, within a broad range of assumed effect sizes, OPV (or another vaccine with NSE) could play an economically attractive role against COVID-19 in countries facing COVID-19 vaccine delays.
FUNDING: The contribution by DTJ was supported through grants from Trond Mohn Foundation (BFS2019MT02) and Norad (RAF-18/0009) through the Bergen Center for Ethics and Priority Setting
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