Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2)

A Hesketh; Andrew Hesketh; CG Hebert; D D'alia; E Wagner; F Flett; Gabriel C. Uguru; GC Uguru; H Aiba; IL Hofacker; J Livny; J Livny; J Pánek; James E. M. Stach; JP Swiercz; L Good; L Good; Liam Good; LS Waters; M McArthur; M McArthur; Madhav Mondhe; Mark Isalan; Mervyn J. Bibb; MP Vockenhuber; MP Vockenhuber; MW Pfaffl; N Nakashima; N Nekhotiaeva; N Shen; NM Romero; NM Romero; P Hindra; R Dryselius; RP Desai; S Goh; S Herai; SG Kang; Shan Goh; T Morita; T Tezuka; TA Hjalt; W Minas; Y Chen

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Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2)

Authors: A Hesketh
Andrew Hesketh
CG Hebert
D D'alia
E Wagner
F Flett
Gabriel C. Uguru
GC Uguru
H Aiba
IL Hofacker
J Livny
J Livny
J Pánek
James E. M. Stach
JP Swiercz
L Good
L Good
Liam Good
LS Waters
M McArthur
M McArthur
Madhav Mondhe
Mark Isalan
Mervyn J. Bibb
MP Vockenhuber
MP Vockenhuber
MW Pfaffl
N Nakashima
N Nekhotiaeva
N Shen
NM Romero
NM Romero
P Hindra
R Dryselius
RP Desai
S Goh
S Herai
SG Kang
Shan Goh
T Morita
T Tezuka
TA Hjalt
W Minas
Y Chen
Publication date: 27 June 2013
Publisher: 'Public Library of Science (PLoS)'
Doi

Abstract

We demonstrate the first application of synthetic RNA gene silencers in Streptomyces coelicolor A3(2). Peptide nucleic acid and expressed antisense RNA silencers successfully inhibited actinorhodin production. Synthetic RNA silencing was target-specific and is a new tool for gene regulation and metabolic engineering studies in Streptomyces.Peer reviewe