Mucus glycoprotein secretion by tracheal explants: effects of pollutants.

Tracheal slices incubated with radioactive precursors in tissue culture medium secrete labeled mucus glycoproteins into the culture medium. We have used an in vivtro approach, a combined method utilizing exposure to pneumotoxins in vivo coupled with quantitation of mucus secretion rates in vitro, to study the effects of inhaled pollutants on mucus biosynthesis by rat airways. In addition, we have purified the mucus glycoproteins secreted by rat tracheal explants in order to determine putative structural changes that might by the basis for the observed augmented secretion rates after exposure of rats to H2SO4 aerosols in combination with high ambient levels of ozone. After digestion with papain, mucus glycoproteins secreted by tracheal explants may be separated into five fractions by ion-exchange chromatography, with recovery in high yield, on columns of DEAE-cellulose. Each of these five fractions, one neutral and four acidic, migrates as a single unique spot upon cellulose acetate electrophoresis at pH values of 8.6 and 1.2. The neutral fraction, which is labeled with [3H] glucosamine, does not contain radioactivity when Na2 35SO4 is used as the precursor. Acidic fractions I-IV are all labeled with either 3H-glucosamine or Na2 35SO4 as precursor. Acidic fraction II contains sialic acid as the terminal sugar on its oligosaccharide side chains, based upon its chromatographic behavior on columns of wheat-germ agglutinin-Agarose. Treatment of this fraction with neuraminidase shifts its elution position in the gradient to a lower salt concentration, coincident with acidic fraction I. After removal of terminal sialic acid residues with either neuraminidase or low pH treatment, the resultant terminal sugar on the oligosaccharide side chains is fucose. These results are identical with those observed with mucus glycoproteins secreted by cultured human tracheal explants and purified by these same techniques

Optimization of nanostructured permalloy electrodes for a lateral hybrid spin-valve structure

Ferromagnetic electrodes of a lateral semiconductor-based spin-valve structure are designed to provide a maximum of spin-polarized injection current. A single-domain state in remanence is a prerequisite obtained by nanostructuring Permalloy thin film electrodes. Three regimes of aspect ratios $m$ are identified by room temperature magnetic force microscopy: (i) high-aspect ratios of $m \ge 20$ provide the favored remanent single-domain magnetization states, (ii) medium-aspect ratios $m \sim 3$ to $m \sim 20$ yield highly remanent states with closure domains and (iii) low-aspect ratios of $m \le 3$ lead to multi-domain structures. Lateral kinks, introduced to bridge the gap between micro- and macroscale, disturb the uniform magnetization of electrodes with high- and medium-aspect ratios. However, vertical flanks help to maintain a uniformly magnetized state at the ferromagnet-semiconcuctor contact by domain wall pinning.Comment: revised version, major structural changes, figures reorganized,6 pages, 8 figures, revte

Onset of Flowering in Biennial and Perennial Garden Plants

Observations were made weekly over a period of 30 years of 208 species (trees, shrubs, herbaceous plants and geophytes) from more than 1,000 growing in a garden located 18km east of the Royal Botanic Garden Edinburgh (RBGE), Scotland (lat. 55º 56ʹN: long. 3º 09ʹW). Of these species, 27 were British native or naturalised.The First Flowering Dates (FFD) of 67 species were without significant temperature association with variable weather; the FFDs of the other 141 species reflected, in contrast, the net outcome of ‘major’ associations with late winter/spring temperatures and smaller impacts of autumn/early winter temperatures. Increases in late winter and spring temperatures advanced the onset of flowering in the current year; in contrast, increases in autumn and early winter temperatures tended to be associated with delayed flowering in the following year.With stepwise regression, penalised signal regression and thermal-time models, it was possible to identify species with ‘strong’ associations with both air and soil temperatures and species with ‘weak’ associations with either air or soil temperatures.Thermal-time models for each of 120 species, whose FFDs were associated with temperature, enabled the characterisation of (1) base temperatures, Tb(°C), at, and above which, development towards open flowers is possible; and (2) thermal constants (degree days accumulated between the start of development and the onset of flowering). Together these attributes suggested that each base temperature cohort has species with widely different degree-day requirements. Between 1978 and 2007 mean air temperatures significantly increased by 0.080°C, 0.044°C and 0.026°C yrˉ¹ in the first, second and third quarters; soil temperatures increased by 0.060ºCyrˉ¹in the first quarter. Over the 30-year period, the trends in flowering showed the early (February/March) flowering species flowering c. 24 days sooner; the later flowering species (April/May) advanced by only c. 12 days

Enhanced ionization in small rare gas clusters

A detailed theoretical investigation of rare gas atom clusters under intense short laser pulses reveals that the mechanism of energy absorption is akin to {\it enhanced ionization} first discovered for diatomic molecules. The phenomenon is robust under changes of the atomic element (neon, argon, krypton, xenon), the number of atoms in the cluster (16 to 30 atoms have been studied) and the fluency of the laser pulse. In contrast to molecules it does not dissappear for circular polarization. We develop an analytical model relating the pulse length for maximum ionization to characteristic parameters of the cluster

Suppressed spin dephasing for 2D and bulk electrons in GaAs wires due to engineered cancellation of spin-orbit interaction terms

We report a study of suppressed spin dephasing for quasi-one-dimensional electron ensembles in wires etched into a GaAs/AlGaAs heterojunction system. Time-resolved Kerr-rotation measurements show a suppression that is most pronounced for wires along the [110] crystal direction. This is the fingerprint of a suppression that is enhanced due to a strong anisotropy in spin-orbit fields that can occur when the Rashba and Dresselhaus contributions are engineered to cancel each other. A surprising observation is that this mechanisms for suppressing spin dephasing is not only effective for electrons in the heterojunction quantum well, but also for electrons in a deeper bulk layer.Comment: 5 pages, 3 figure

Double butterfly spectrum for two interacting particles in the Harper model

We study the effect of interparticle interaction $U$ on the spectrum of the Harper model and show that it leads to a pure-point component arising from the multifractal spectrum of non interacting problem. Our numerical studies allow to understand the global structure of the spectrum. Analytical approach developed permits to understand the origin of localized states in the limit of strong interaction $U$ and fine spectral structure for small $U$.Comment: revtex, 4 pages, 5 figure

Dynamical ionization ignition of clusters in intense and short laser pulses

The electron dynamics of rare gas clusters in laser fields is investigated quantum mechanically by means of time-dependent density functional theory. The mechanism of early inner and outer ionization is revealed. The formation of an electron wave packet inside the cluster shortly after the first removal of a small amount of electron density is observed. By collisions with the cluster boundary the wave packet oscillation is driven into resonance with the laser field, hence leading to higher absorption of laser energy. Inner ionization is increased because the electric field of the bouncing electron wave packet adds up constructively to the laser field. The fastest electrons in the wave packet escape from the cluster as a whole so that outer ionization is increased as well.Comment: 8 pages, revtex4, PDF-file with high resolution figures is available from http://mitarbeiter.mbi-berlin.de/bauer/publist.html, publication no. 24. Accepted for publication in Phys. Rev.

Acceptance conditions in automated negotiation

In every negotiation with a deadline, one of the negotiating parties has to accept an offer to avoid a break off. A break off is usually an undesirable outcome for both parties, therefore it is important that a negotiator employs a proficient mechanism to decide under which conditions to accept. When designing such conditions one is faced with the acceptance dilemma: accepting the current offer may be suboptimal, as better offers may still be presented. On the other hand, accepting too late may prevent an agreement from being reached, resulting in a break off with no gain for either party. Motivated by the challenges of bilateral negotiations between automated agents and by the results and insights of the automated negotiating agents competition (ANAC), we classify and compare state-of-the-art generic acceptance conditions. We focus on decoupled acceptance conditions, i.e. conditions that do not depend on the bidding strategy that is used. We performed extensive experiments to compare the performance of acceptance conditions in combination with a broad range of bidding strategies and negotiation domains. Furthermore we propose new acceptance conditions and we demonstrate that they outperform the other conditions that we study. In particular, it is shown that they outperform the standard acceptance condition of comparing the current offer with the offer the agent is ready to send out. We also provide insight in to why some conditions work better than others and investigate correlations between the properties of the negotiation environment and the efficacy of acceptance condition

X-ray Phase-Contrast Imaging and Metrology through Unified Modulated Pattern Analysis

We present a method for x-ray phase-contrast imaging and metrology applications based on the sample-induced modulation and subsequent computational demodulation of a random or periodic reference interference pattern. The proposed unified modulated pattern analysis (UMPA) technique is a versatile approach and allows tuning of signal sensitivity, spatial resolution, and scan time. We characterize the method and demonstrate its potential for high-sensitivity, quantitative phase imaging, and metrology to overcome the limitations of existing methods