An update of information on market characteristics of sweet potato in the Lake and Eastern Zones of Tanzania

This report is an update of information on market characteristics of sweet potato conducted in the Lake and Eastern zones of Tanzania. It is intended to provide information under which flexible systems that are required to enable farmers to market their crops either when they need income or when market prices are most favorable. Information was obtained by interviewing farmers and traders from Lake Zone and Eastern Zone of Tanzania using Rapid Rural Appraisal method (RRA). Groups of 12 to 14 farmers, one from each village surveyed were selected randomly for focus group interviews. Each group comprised of women and men. Traders from the municipal markets of Dar es Salaam, Morogoro and Mwanza were also interviewed. This information was complemented by information from the District Agricultural Development Office. Groups of three Scientists (a post-harvest specialist, a market specialist and a social worker) undertook the interviews. The report documents the economic activities of the farmers and types of crops grown in the respective areas. It specifically provides information on the production, handling and marketing constraints of sweet potatoes. Other aspects shown in the report include, the marketing chain of sweet potatoes, production and marketing costs of the crop and seasonality of supply of sweet potatoes versus prices and quality in Mwanza and Dares Salaam markets. A number of recommendations based on the overall observation of the study have been documented in order to enable the stakeholders of the project to plan further interventions

The use of needs assessment methodologies to focus technical interventions in root and tuber crop post-harvest systems: a case study to improve incomes and reduce losses associated with marketing of fresh cassava from rural areas to Dar es Salaam

The Transfer of Needs Assessment Methodologies and Post-Harvest Technologies for Non-Grain Starch Staple Food Crops (NGSS) in sub-Saharan Africa project was initiated in Tanzania in July 1993 as a collaborative venture between the Natural Resources Institute (NRI) and the Tanzania Food and Nutrition Centre (TFNC). The project was funded by the UK Department For International Development (DFID). The main project involved activities in Ghana and Tanzania but this report deals exclusively with activities in Tanzania focusing on the transfer of needs assessment methodologies, the application of needs assessment in identifying post-harvest problems and technical interventions for NGSS and the identification, validation and dissemination of a technology to improve incomes and reduce post-harvest losses associated with marketing of fresh cassava roots from rural production areas to urban markets in Dar es Salaam. In the first stage of the project a range of needs assessment methodologies were successfully validated under field conditions through the mechanism of three case studies, which were carried out in Lake Zone, Tanga Region and Dar es Salaam of Tanzania. Following the case studies, two training in needs assessment workshops were conducted in Tanzania to disseminate needs assessment technologies to key personnel from research organisations and institutes in Tanzania, Mozambique, Uganda, Kenya, Ghana, Rwanda and Ethiopia. The needs assessment case study in Tanga Region highlighted post-harvest problems as a major constraint facing farmers and traders involved in the marketing of fresh cassava to urban areas, and formed the basis for a more detailed study of the marketing system for fresh cassava from rural production areas to urban markets in Dar es Salaam. Semi structured interviews with key stakeholders in the marketing system demonstrated that reductions in quality resulting from poor handling and delays in marketing lead to serious reductions in income throughout the market chain. Farmers and market personnel identified the criteria for a technical intervention to reduce income losses incurred when marketing fresh cassava. After considering various options a simple low cost storage technique for fresh cassava originally developed by the Centro Internacional de Agricultura Tropical (CIAT) and NRI and later modified with DFID RNRRS funds by the NRI and the Ministry of Food and Agriculture (MoFA) in Ghana was selected for validation in Tanzania. Validation exercises in Tanzania demonstrated that low cost fresh cassava root storage technology had the technical and economic potential to alleviate postharvest problems associated with marketing of fresh cassava and improve the incomes of those involved in the cassava market system. In the final stage of the project a flexible dissemination strategy was developed and used to carry out a limited technical dissemination campaign in the major cassava markets in Dares Salaam and selected villages ofKisarawe and Ruf~i Districts in Pwani (Coast) Region, and to promote wider awareness of the technology throughout Tanzania. Needs assessment techniques were used to develop practical approaches for dissemination and two impact assessments (November 1996 and December1997) were carried out to assess effectiveness of dissemination activities and allow for optimisation ofthe strategy. The first impact assessment (November 1996) was used to highlight the strengths and limitations of the initial dissemination strategy, so that a more effective strategy could be developed in collaboration with representatives of the Agricultural Extension Services and key stakeholders in the market system. In December 1997 a second impact assessment was made to assess the effectiveness of the revised dissemination strategy and to highlight any problems. The wider awareness campaign was found to have been effective and a number of expressions of interest had been received from organisations and individuals interested in fresh cassava within Tanzania. A high level of interest and awareness was found in markets and villages where training activities had taken place, but a number of problems were identified which were hampering uptake of the technology. The two main problems were: (a). to be effective the technology needs to be implemented at all points in the market system but individuals were unwilling to take the risk ofbeing the first to use the technology without proof of the economic benefits of the technology; (b). the market system is dominated by a large open type of package called a lumbesa which is not suitable for application of the technology or for transport of cassava but is used for other reasons related to transport charges. To overcome the first difficulty the project team developed a marketing demonstration to provide a practical demonstration of the financial benefits of low cost :fresh cassava root storage technology to potential beneficiaries within the marketing system. The second difficulty was addressed by representatives of various village governments and market co-operatives in collaboration with the TFNCINRI project team. In November of 1997 the village governments of Jaribu and Bungu villages in Rufiji District enacted bylaws to encourage adoption of close packaging for cassava and adoption of the new storage technology. To support this initiative the Agricultural Extension Service in Rufiji District have agreed to integrate the technology into their extension programmes, and in addition the village government in Jaribu has initiated its own training programme in the sub-villages around Jaribu. In December 1997 the village governments of Masaki, Sungwi and Gumba villages in Kisarawe District villages decided to follow the lead taken by villages in Rufiji District and develop bylaws and training programmes to promote uptake ofthe new technology. The Agricultural Extension Service for Kisarawe District have acknowledged the importance of cassava as a source of income generation in the District, and have agreed to integrate the technology into their extension programmes. If co-operation between the various stakeholders is maintained it seems likely that the technology will start to be adopted during 1998. This case study within the main regional Africa project has demonstrated not only that low cost :fresh cassava root storage technology has the technical and economic potential to reduce post-harvest losses and improve incomes, but has also shown that key stakeholders are genuinely interested in adoption of the technology and are willing to co-operate and spend their own funds to promote uptake of the technology. However, for successful uptake of the technology a wider dissemination at both local and national level is required. It is also clear that lack of funds in rural areas could constrain producers and country buyers from adopting this technology. To meet the needs ofthose involved in marketing of fresh cassava throughout Tanzania a sustained campaign of dissemination is required, involving close collaboration between the Agricultural Extension Services, selected NGOs and key stakeholders involved in marketing of fresh cassava from rural areas to various urban centres in Tanzania. A concept note for wider dissemination of low cost fresh cassava root storage technology in Tanzania is included in this report (Appendix 5). The fmdings of this case study have shown that successful adoption of this technology would improve the quality of cassava reaching urban consumers, and contribute to poverty alleviation by improving the income generating potential of marketing of fresh cassava

Cardiac Resynchronization With Sequential Biventricular Pacing for the Treatment of Moderate-to-Severe Heart Failure

ObjectivesThe InSync III study evaluated sequential cardiac resynchronization therapy (CRT) in patients with moderate-to-severe heart failure and prolonged QRS.BackgroundSimultaneous CRT improves hemodynamic and clinical performance in patients with moderate-to-severe heart failure (HF) and a wide QRS. Recent evidence suggests that sequentially stimulating the ventricles might provide additional benefit.MethodsThis multicenter, prospective, nonrandomized, six-month trial enrolled a total of 422 patients to determine the effectiveness of sequential CRT in patients with New York Heart Association (NYHA) functional class III or IV HF and a prolonged QRS. The study evaluated: whether patients receiving sequential CRT for six months experienced improvement in 6-min hall walk (6MHW) distance, NYHA functional class, and quality of life (QoL) over control group patients from the reported Multicenter InSync Randomized Clinical Evaluation (MIRACLE) trial; whether sequential CRT increased stroke volume compared to simultaneous CRT; and whether an increase in stroke volume translated into greater clinical improvements compared to patients receiving simultaneous CRT.ResultsInSync III patients experienced greater improvement in 6MHW, NYHA functional class, and QoL at six months compared to control (all p < 0.0001). Optimization of the sequential pacing increased (median 7.3%) stroke volume in 77% of patients. No additional improvement in NYHA functional class or QoL was seen compared to the simultaneous CRT group; however, InSync III patients demonstrated greater exercise capacity.ConclusionsSequential CRT provided most patients with a modest increase in stroke volume above that achieved during simultaneous CRT. Patients receiving sequential CRT had improved exercise capacity, but no change in functional status or QoL

Creative and Stylistic Devices Employed by Children During a Storybook Narrative Task: A Cross-Cultural Study

Purpose: The purpose of this study was to analyze the effects of culture on the creative and stylistic features children employ when producing narratives based on wordless picture books. Method: Participants included 60 first- and second-grade African American, Latino American, and Caucasian children. A subset of narratives based on wordless picture books collected as part of a larger study was coded and analyzed for the following creative and stylistic conventions: organizational style (topic centered, linear, cyclical), dialogue (direct, indirect), reference to character relationships (nature, naming, conduct), embellishment (fantasy, suspense, conflict), and paralinguistic devices (expressive sounds, exclamatory utterances). Results: Many similarities and differences between ethnic groups were found. No significant differences were found between ethnic groups in organizational style or use of paralinguistic devices. African American children included more fantasy in their stories, Latino children named their characters more often, and Caucasian children made more references to the nature of character relationships. Conclusion: Even within the context of a highly structured narrative task based on wordless picture books, culture influences children’s production of narratives. Enhanced understanding of narrative structure, creativity, and style is necessary to provide ecologically valid narrative assessment and intervention for children from diverse cultural backgrounds

Human cryptochrome exhibits light-dependent magnetosensitivity

Humans are not believed to have a magnetic sense, even though many animals use the Earth's magnetic field for orientation and navigation. One model of magnetosensing in animals proposes that geomagnetic fields are perceived by light-sensitive chemical reactions involving the flavoprotein cryptochrome (CRY). Here we show using a transgenic approach that human CRY2, which is heavily expressed in the retina, can function as a magnetosensor in the magnetoreception system of Drosophila and that it does so in a light-dependent manner. The results show that human CRY2 has the molecular capability to function as a light-sensitive magnetosensor and reopen an area of sensory biology that is ready for further exploration in humans

Resistance to the CCR5 Inhibitor 5P12-RANTES Requires a Difficult Evolution from CCR5 to CXCR4 Coreceptor Use

Viral resistance to small molecule allosteric inhibitors of CCR5 is well documented, and involves either selection of preexisting CXCR4-using HIV-1 variants or envelope sequence evolution to use inhibitor-bound CCR5 for entry. Resistance to macromolecular CCR5 inhibitors has been more difficult to demonstrate, although selection of CXCR4-using variants might be expected. We have compared the in vitro selection of HIV-1 CC1/85 variants resistant to either the small molecule inhibitor maraviroc (MVC) or the macromolecular inhibitor 5P12-RANTES. High level resistance to MVC was conferred by the same envelope mutations as previously reported after 16–18 weeks of selection by increasing levels of MVC. The MVC-resistant mutants were fully sensitive to inhibition by 5P12-RANTES. By contrast, only transient and low level resistance to 5P12-RANTES was achieved in three sequential selection experiments, and each resulted in a subsequent collapse of virus replication. A fourth round of selection by 5P12-RANTES led, after 36 weeks, to a “resistant” variant that had switched from CCR5 to CXCR4 as a coreceptor. Envelope sequences diverged by 3.8% during selection of the 5P12-RANTES resistant, CXCR4-using variants, with unique and critical substitutions in the V3 region. A subset of viruses recovered from control cultures after 44 weeks of passage in the absence of inhibitors also evolved to use CXCR4, although with fewer and different envelope mutations. Control cultures contained both viruses that evolved to use CXCR4 by deleting four amino acids in V3, and others that maintained entry via CCR5. These results suggest that coreceptor switching may be the only route to resistance for compounds like 5P12-RANTES. This pathway requires more mutations and encounters more fitness obstacles than development of resistance to MVC, confirming the clinical observations that resistance to small molecule CCR5 inhibitors very rarely involves coreceptor switching

Genotypic tropism testing by massively parallel sequencing: qualitative and quantitative analysis

<p>Abstract</p> <p>Background</p> <p>Inferring viral tropism from genotype is a fast and inexpensive alternative to phenotypic testing. While being highly predictive when performed on clonal samples, sensitivity of predicting CXCR4-using (X4) variants drops substantially in clinical isolates. This is mainly attributed to minor variants not detected by standard bulk-sequencing. Massively parallel sequencing (MPS) detects single clones thereby being much more sensitive. Using this technology we wanted to improve genotypic prediction of coreceptor usage.</p> <p>Methods</p> <p>Plasma samples from 55 antiretroviral-treated patients tested for coreceptor usage with the Monogram Trofile Assay were sequenced with standard population-based approaches. Fourteen of these samples were selected for further analysis with MPS. Tropism was predicted from each sequence with geno2pheno_[coreceptor].</p> <p>Results</p> <p>Prediction based on bulk-sequencing yielded 59.1% sensitivity and 90.9% specificity compared to the trofile assay. With MPS, 7600 reads were generated on average per isolate. Minorities of sequences with high confidence in CXCR4-usage were found in all samples, irrespective of phenotype. When using the default false-positive-rate of geno2pheno_[coreceptor](10%), and defining a minority cutoff of 5%, the results were concordant in all but one isolate.</p> <p>Conclusions</p> <p>The combination of MPS and coreceptor usage prediction results in a fast and accurate alternative to phenotypic assays. The detection of X4-viruses in all isolates suggests that coreceptor usage as well as fitness of minorities is important for therapy outcome. The high sensitivity of this technology in combination with a quantitative description of the viral population may allow implementing meaningful cutoffs for predicting response to CCR5-antagonists in the presence of X4-minorities.</p

Stitching a new garment: considering the future of the speech–language therapy profession globally

Providing equitable support for people experiencing communication disability (CD) globally is a historical and contemporary challenge for the speech–language therapy profession. A group of speech–language therapists (SLTs) with ongoing and sustained experiences in Majority and Minority World contexts participated in five virtual meetings in 2021. The aim of these meetings was to develop provocative statements that might spur a global discussion among individuals and organisations that support people experiencing CD. The following questions were discussed: What is our vision for the future of the profession globally? What are the global challenges around access to speech–language therapy services? Four main themes emerged: (1) the need to centre people experiencing CD as the focal point of services, (2) participation, (3) equity and (4) community. The themes relate to the need for a process of de-imperialism in the profession. Suggestions were made to develop more suitable terminology and to establish a global framework that promotes more equitable access to communication services. We seek the adoption of approaches that focus on reciprocal global engagement for capacity strengthening. Alternative models of culturally sustaining and equitable service delivery are needed to create impact for people experiencing CD, and their families worldwide. Contribution: Provocative statements were developed to prompt global conversations among speech–language therapy professionals and associations. We encourage readers to consider the questions posed, share their viewpoints and initiate positive change towards a global strategy

The Evolutionary Analysis of Emerging Low Frequency HIV-1 CXCR4 Using Variants through Time—An Ultra-Deep Approach

Large-scale parallel pyrosequencing produces unprecedented quantities of sequence data. However, when generated from viral populations current mapping software is inadequate for dealing with the high levels of variation present, resulting in the potential for biased data loss. In order to apply the 454 Life Sciences' pyrosequencing system to the study of viral populations, we have developed software for the processing of highly variable sequence data. Here we demonstrate our software by analyzing two temporally sampled HIV-1 intra-patient datasets from a clinical study of maraviroc. This drug binds the CCR5 coreceptor, thus preventing HIV-1 infection of the cell. The objective is to determine viral tropism (CCR5 versus CXCR4 usage) and track the evolution of minority CXCR4-using variants that may limit the response to a maraviroc-containing treatment regimen. Five time points (two prior to treatment) were available from each patient. We first quantify the effects of divergence on initial read k-mer mapping and demonstrate the importance of utilizing population-specific template sequences in relation to the analysis of next-generation sequence data. Then, in conjunction with coreceptor prediction algorithms that infer HIV tropism, our software was used to quantify the viral population structure pre- and post-treatment. In both cases, low frequency CXCR4-using variants (2.5–15%) were detected prior to treatment. Following phylogenetic inference, these variants were observed to exist as distinct lineages that were maintained through time. Our analysis, thus confirms the role of pre-existing CXCR4-using virus in the emergence of maraviroc-insensitive HIV. The software will have utility for the study of intra-host viral diversity and evolution of other fast evolving viruses, and is available from http://www.bioinf.manchester.ac.uk/segminator/