Dynamics of Microtubule Networks with Antiparallel Crosslinkers

Microtubules are the most rigid element of the cytoskeleton. They are responsible for the structure of cells and make up the tracks for intracellular cargo transport. Interactions between microtubules, motor proteins, and microtubule-associated proteins drive important mechanisms in the cell, such as cell division, cell motility, cell homeostasis, and cell signaling. I seek to understand how such complex, energy-consuming non-equilibrium biological networks self-organize by studying in vitro microtubules bundled by microtubule-associated protein 65 (MAP65), in kinesin-1 gliding assays. I found that large networks can break into smaller, cell-like networks that can mimic types of cell motility. Dynamics of these networks change with varying concentrations of MAP65 and microtubules

Genome characterization and population genetic structure of the zoonotic pathogen, streptococcus canis

Background - Streptococcus canis is an important opportunistic pathogen of dogs and cats that can also infect a wide range of additional mammals including cows where it can cause mastitis. It is also an emerging human pathogen. Results - Here we provide characterization of the first genome sequence for this species, strain FSL S3-227 (milk isolate from a cow with an intra-mammary infection). A diverse array of putative virulence factors was encoded by the S. canis FSL S3-227 genome. Approximately 75% of these gene sequences were homologous to known Streptococcal virulence factors involved in invasion, evasion, and colonization. Present in the genome are multiple potentially mobile genetic elements (MGEs) [plasmid, phage, integrative conjugative element (ICE)] and comparison to other species provided convincing evidence for lateral gene transfer (LGT) between S. canis and two additional bovine mastitis causing pathogens (Streptococcus agalactiae, and Streptococcus dysgalactiae subsp. dysgalactiae), with this transfer possibly contributing to host adaptation. Population structure among isolates obtained from Europe and USA [bovine = 56, canine = 26, and feline = 1] was explored. Ribotyping of all isolates and multi locus sequence typing (MLST) of a subset of the isolates (n = 45) detected significant differentiation between bovine and canine isolates (Fisher exact test: P = 0.0000 [ribotypes], P = 0.0030 [sequence types]), suggesting possible host adaptation of some genotypes. Concurrently, the ancestral clonal complex (54% of isolates) occurred in many tissue types, all hosts, and all geographic locations suggesting the possibility of a wide and diverse niche. Conclusion - This study provides evidence highlighting the importance of LGT in the evolution of the bacteria S. canis, specifically, its possible role in host adaptation and acquisition of virulence factors. Furthermore, recent LGT detected between S. canis and human bacteria (Streptococcus urinalis) is cause for concern, as it highlights the possibility for continued acquisition of human virulence factors for this emerging zoonotic pathogen

Spectral geometry of the Steklov problem on orbifolds

We consider how the geometry and topology of a compact $n$-dimensional Riemannian orbifold with boundary relates to its Steklov spectrum. In two dimensions, motivated by work of A. Girouard, L. Parnovski, I. Polterovich and D. Sher in the manifold setting, we compute the precise asymptotics of the Steklov spectrum in terms of only boundary data. As a consequence, we prove that the Steklov spectrum detects the presence and number of orbifold singularities on the boundary of an orbisurface and it detects the number each of smooth and singular boundary components. Moreover, we find that the Steklov spectrum also determines the lengths of the boundary components modulo an equivalence relation, and we show by examples that this result is the best possible. We construct various examples of Steklov isospectral Riemannian orbifolds which demonstrate that these two-dimensional results do not extend to higher dimensions. In addition, we give two- imensional examples which show that the Steklov spectrum does \emph{not} detect the presence of interior singularities nor does it determine the orbifold Euler characteristic. In fact, a flat disk is Steklov isospectral to a cone. In another direction, we obtain upper bounds on the Steklov eigenvalues of a Riemannian orbifold in terms of the isoperimetric ratio and a conformal invariant. We generalize results of B. Colbois, A. El Soufi and A. Girouard, and the fourth author to the orbifold setting; in the process, we gain a sharpness result on these bounds that was not evident in the manifold setting. In dimension two, our eigenvalue bounds are solely in terms of the orbifold Euler characteristic and the number each of smooth and singular boundary components

E-letter: Follow-up after arthroplasty of the hip and knee: Are we over-servicing or under-caring?

© 2018 The British Editorial Society of Bone & Joint Surgery. The number of arthroplasties of the hip and knee is predicted to increase rapidly during the next 20 years. Accompanying this is the dilemma of how to follow-up these patients appropriately. Current guidelines recommend long-term follow-up to identify patients with aseptic loosening, which can occur more than a decade postoperatively. The current guidelines and practices of orthopaedic surgeons vary widely. Existing models take up much clinical time and are expensive. Pilot studies using 'virtual' clinics and advanced-practice physiotherapists have shown promise in decreasing the time and costs for orthopaedic surgeons and patients. This review discusses current practices and future trends in the follow-up of patients who have an arthroplasty

Understanding person acquisition using an interactive activation and competition network

Face perception is one of the most developed visual skills that humans display, and recent work has attempted to examine the mechanisms involved in face perception through noting how neural networks achieve the same performance. The purpose of the present paper is to extend this approach to look not just at human face recognition, but also at human face acquisition. Experiment 1 presents empirical data to describe the acquisition over time of appropriate representations for newly encountered faces. These results are compared with those of Simulation 1, in which a modified IAC network capable of modelling the acquisition process is generated. Experiment 2 and Simulation 2 explore the mechanisms of learning further, and it is demonstrated that the acquisition of a set of associated new facts is easier than the acquisition of individual facts in isolation of one another. This is explained in terms of the advantage gained from additional inputs and mutual reinforcement of developing links within an interactive neural network system. <br/

Religious faith and psychosocial adaptation among stroke patients in Kuwait: A mixed method study

This is the author's accepted manuscript. The final published article is available from the link below. Copyright @ 2012 Springer Science+Business Media.Religious faith is central to life for Muslim patients in Kuwait, so it may influence adaptation and rehabilitation. This study explored quantitative associations among religious faith, self-efficacy, and life satisfaction in 40 female stroke patients and explored the influence of religion within stroke rehabilitation through qualitative interviews with 12 health professionals. The quantitative measure of religious faith did not relate to life satisfaction or self-efficacy in stroke patients. However, the health professionals described religious coping as influencing adaptation post-stroke. Fatalistic beliefs were thought to have mixed influences on rehabilitation. Measuring religious faith among Muslims through a standardized scale is debated. The qualitative accounts suggest that religious beliefs need to be acknowledged in stroke rehabilitation in Kuwait

Classical and quantum ergodicity on orbifolds

We extend to orbifolds classical results on quantum ergodicity due to Shnirelman, Colin de Verdi\`ere and Zelditch, proving that, for any positive, first-order self-adjoint elliptic pseudodifferential operator P on a compact orbifold X with positive principal symbol p, ergodicity of the Hamiltonian flow of p implies quantum ergodicity for the operator P. We also prove ergodicity of the geodesic flow on a compact Riemannian orbifold of negative sectional curvature.Comment: 14 page