Wider view over bicycle crashes: Complementing and extending bicycle crash statistics in urban areas using surveys

ntroduction: In pursuit of sustainability goals, many cities are introducing measures to increase the usage of bicycles as a means of transportation. City planners aim to ensure that this increase does not lead to an increase in crashes, but must make corresponding infrastructure decisions with limited information. Sufficient data to perform a statistical analysis of location-specific crash frequencies is rarely available. For example, only approximately 10% of all bicycle crashes are reported to the police (Shinar et al., 2018). Therefore, urban planners often rely on expert opinion, which may lead to suboptimal prioritization and realization of infrastructure improvements. Method: This paper demonstrates how surveys on bicycle crashes can be used to aid urban planners in making infrastructure decisions. In addition to confirming the location and characteristics of reported crashes, surveys can uncover characteristics of crashes that are not reported to the police, situations in which a crash almost occurred, and locations perceived by cyclists to be dangerous. Surveys also allow urban planners to investigate non-infrastructure related causes of crashes, such as the frequency with which individual cyclists use other modes of transportation. Practical Applications: The usefulness of surveys in the determination of urban cycling safety is demonstrated in this paper through analysis of survey results from the city of Zurich in 2018

Wider view over bicycle accidents: Complementing and extending bicycle accident statistics in urban areas using surveys

City traffic planners are striving to adapt their infrastructure to not only increase the number of cyclists but also to ensure that city cycling is both enjoyable and safe. In Switzerland and in many other countries, it is suspected that only one of ten bicycle accidents is reported to the police [ 1-5]. Only knowing about 10% of the accidents, on top of the fact that there are luckily not many accidents from a statistical perspective, casts doubt about where efforts should be made to improve cycling infrastructure, and how effective the actions taken actually are. To deal with this lack of data, this paper proposes to use surveys of cyclists besides police records to obtain a more complete picture of the number and location of cycling accidents, including the ones not reported to police, and the locations that cyclists perceive as dangerous. The combination of survey and police reported data gives a considerably different and more complete impression of where there is potential to improve cycling infrastructure, when compared to that obtained using only police reported accidents. This work expounds how the survey responses about hazard perception and unreported accidents help provide a more complete overview of the accident potential of the existing cycling network and how they form a base of immensely useful inputs for planning improvements. [From: Introduction

Complex Networks on Hyperbolic Surfaces

We explore a novel method to generate and characterize complex networks by means of their embedding on hyperbolic surfaces. Evolution through local elementary moves allows the exploration of the ensemble of networks which share common embeddings and consequently share similar hierarchical properties. This method provides a new perspective to classify network-complexity both on local and global scale. We demonstrate by means of several examples that there is a strong relation between the network properties and the embedding surface.Comment: 8 Pages 3 Figure

Stability conditions and Stokes factors

Let A be the category of modules over a complex, finite-dimensional algebra. We show that the space of stability conditions on A parametrises an isomonodromic family of irregular connections on P^1 with values in the Hall algebra of A. The residues of these connections are given by the holomorphic generating function for counting invariants in A constructed by D. Joyce.Comment: Very minor changes. Final version. To appear in Inventione

Exploring complex networks via topological embedding on surfaces

We demonstrate that graphs embedded on surfaces are a powerful and practical tool to generate, characterize and simulate networks with a broad range of properties. Remarkably, the study of topologically embedded graphs is non-restrictive because any network can be embedded on a surface with sufficiently high genus. The local properties of the network are affected by the surface genus which, for example, produces significant changes in the degree distribution and in the clustering coefficient. The global properties of the graph are also strongly affected by the surface genus which is constraining the degree of interwoveness, changing the scaling properties from large-world-kind (small genus) to small- and ultra-small-world-kind (large genus). Two elementary moves allow the exploration of all networks embeddable on a given surface and naturally introduce a tool to develop a statistical mechanics description. Within such a framework, we study the properties of topologically-embedded graphs at high and low `temperatures' observing the formation of increasingly regular structures by cooling the system. We show that the cooling dynamics is strongly affected by the surface genus with the manifestation of a glassy-like freezing transitions occurring when the amount of topological disorder is low.Comment: 18 pages, 7 figure

Alterations in composition and diversity of the intestinal microbiota in patients with diarrhea-predominant irritable bowel syndrome: Alterations in composition and diversity of the intestinal microbiota in D-IBS

The intestinal microbiota has been implicated in the pathophysiology of Irritable Bowel Syndrome (IBS). Due to the variable resolutions of techniques used to characterize the intestinal microbiota, and the heterogeneity of IBS, the defined alterations of the IBS intestinal microbiota are inconsistent. We analyzed the composition of the intestinal microbiota in a defined subgroup of IBS patients (diarrhea-predominant IBS, D-IBS) using a technique that provides the deepest characterization available for complex microbial communities

The biosynthesis of pyoverdines

Pyoverdines are fluorescent siderophores of pseudomonads that play important roles for growth under iron-limiting conditions. The production of pyoverdines by fluorescent pseudomonads permits their colonization of hosts ranging from humans to plants. Prominent examples include pathogenic or non-pathogenic species such as Pseudomonas aeruginosa, P. putida, P. syringae, or P. fluorescens. Many distinct pyoverdines have been identified, all of which have a dihydroxyquinoline fluorophore in common, derived from oxidative cyclizations of non-ribosomal peptides. These serve as precursor of pyoverdines and are commonly known as ferribactins. Ferribactins of distinct species or even strains often differ in their sequence, resulting in a large variety of pyoverdines. However, synthesis of all ferribactins begins with an L-Glu/D-Tyr/L-Dab sequence, and the fluorophore is generated from the D-Tyr/L-Dab residues. In addition, the initial L-Glu residue is modified to various acids and amides that are responsible for the range of distinguishable pyoverdines in individual strains. While ferribactin synthesis is a cytoplasmic process, the maturation to the fluorescent pyoverdine as well as the tailoring of the initial glutamate are exclusively periplasmic processes that have been a mystery until recently. Here we review the current knowledge of pyoverdine biosynthesis with a focus on the recent advancements regarding the periplasmic maturation and tailoring reactions

PvdM of fluorescent pseudomonads is required for the oxidation of ferribactin by PvdP in periplasmic pyoverdine maturation

Fluorescent pseudomonads such as Pseudomonas aeruginosa or Pseudomonas fluorescens produce pyoverdine siderophores that ensure iron-supply in iron-limited environments. After its synthesis in the cytoplasm, the nonfluorescent pyoverdine precursor ferribactin is exported into the periplasm, where the enzymes PvdQ, PvdP, PvdO, PvdN, and PtaA are responsible for fluorophore maturation and tailoring steps. While the roles of all these enzymes are clear, little is known about the role of PvdM, a human renal dipeptidase–related protein that is predicted to be periplasmic and that is essential for pyoverdine biogenesis. Here, we reveal the subcellular localization and functional role of PvdM. Using the model organism P. fluorescens, we show that PvdM is anchored to the periplasmic side of the cytoplasmic membrane, where it is indispensable for the activity of the tyrosinase PvdP. While PvdM does not share the metallopeptidase function of renal dipeptidase, it still has the corresponding peptide-binding site. The substrate of PvdP, deacylated ferribactin, is secreted by a ΔpvdM mutant strain, indicating that PvdM prevents loss of this periplasmic biosynthesis intermediate into the medium by ensuring the efficient transfer of ferribactin to PvdP in vivo. We propose that PvdM belongs to a new dipeptidase-related protein subfamily with inactivated Zn2+ coordination sites, members of which are usually genetically linked to TonB-dependent uptake systems and often associated with periplasmic FAD-dependent oxidoreductases related to D-amino acid oxidases. We suggest that these proteins are necessary for selective binding, exposure, or transfer of specific D- and L-amino acid–containing peptides and other periplasmic biomolecules in manifold pathways

Understanding the competing pathways leading to hydropyrene and isoelisabethatriene

Terpene synthases are responsible for the biosynthesis of terpenes, the largest family of natural products. Hydropyrene synthase generates hydropyrene and hydropyrenol as its main products along with two byproducts, isoelisabethatrienes A and B. Fascinatingly, a single active site mutation (M75L) diverts the product distribution towards isoelisabethatrienes A and B. In the current work, we study the competing pathways leading to these products using quantum chemical calculations in the gas phase. We show that there is a great thermodynamic preference for hydropyrene and hydropyrenol formation, and hence most likely in the synthesis of the isoelisabethatriene products kinetic control is at play