Fabrication of a Hybrid Microfluidic System Incorporating both Lithographically Patterned Microchannels and a 3D Fiber-Formed Microfluidic Network

A device containing a 3D microchannel network (fabricated using sacrificial melt-spun microfibers) sandwiched between lithographically patterned microfluidic channels offers improved delivery of soluble compounds to a large volume compared to a simple stack of two microfluidic channel layers. With this improved delivery ability comes an increased fluidic resistance due to the tortuous network of small-diameter channels.United States. Army (Engineer Research and Development Center-Construction Engineering Research Laboratory (ERDC-CERL))National Institutes of Health (U.S.) (NIH grant 5F32EB011866)National Institutes of Health (U.S.) (NIH Grant 1K99EB013630)National Institutes of Health (U.S.) (NIH NHLBI grant 1 R21 HL106585-01)National Heart, Lung, and Blood Institute (NIH NHLBI grant 1 R21 HL106585-01

The Hamburg/SAO Survey for Emission--Line Galaxies. IV. The Fourth List of 119 Galaxies

We present the fourth list with results of the Hamburg/SAO Survey for Emission-Line Galaxies (HSS hereafter, SAO - Special Astrophysical Observatory, Russia). The list is a result of the follow-up spectroscopy conducted with the 6m SAO RAS telescope in 1998, 1999 and 2000. The data of this snap-shot spectroscopy survey confirmed 127 emission-line objects out of 176 observed candidates and allowed their quantitative spectral classification. We could classify 76 emission-line objects as BCG/HII galaxies or probable BCGs, 8 - as QSOs, 2 - as Seyfert galaxies, 2 - as super-associations in a subluminous spiral and an irregular galaxy, and 37 as low-excitation objects - either starburst nuclei (SBN), or dwarf amorphous nuclei starburst galaxies (DANS). We could not classify 2 ELGs. Furthermore, for 5 galaxies we did not detect any significant emission lines. For 91 emission-line galaxies, the redshifts and/or line intensities are determined for the first time. Of the remaining 28 previously known ELGs we give either improved data on the line intensities or some independent measurements. The candidates were taken from three different samples selected by different criteria. Among our first priority candidates we achieved a detection rate of emission-line objects (ELGs + QSOs) of 68%, among which 51% are BCGs. Observations of a random selected sample among our second priority candidates showed that only ~10% are BCGs. We found that the confirmed BCGs have usually a blue colour ((B-R) < 1.0) and a non-stellar appearance in the APM database. Our third sample is comprised of second priority candidates fulfilling these criteria derived from the APM. Follow-up spectroscopy of a small subsample indicates that the expected detection rate for BCGs is ~40%.Comment: 15 pages, 1 embedded figure and A1-A12(spectra), A&AS in press. PS-file of A1-A12(spectra) also available via http://precise.sao.ru/Laboratory/Publications/2000/HSSIV_figs.ps.g

P-Type ATPase TAT-2 Negatively Regulates Monomethyl Branched-Chain Fatty Acid Mediated Function in Post-Embryonic Growth and Development in C. elegans

Monomethyl branched-chain fatty acids (mmBCFAs) are essential for Caenorhabditis elegans growth and development. To identify factors acting downstream of mmBCFAs for their function in growth regulation, we conducted a genetic screen for suppressors of the L1 arrest that occurs in animals depleted of the 17-carbon mmBCFA C17ISO. Three of the suppressor mutations defined an unexpected player, the P-type ATPase TAT-2, which belongs to the flippase family of proteins that are implicated in mediating phospholipid bilayer asymmetry. We provide evidence that TAT-2, but not other TAT genes, has a specific role in antagonizing the regulatory activity of mmBCFAs in intestinal cells. Interestingly, we found that mutations in tat-2 also suppress the lethality caused by inhibition of the first step in sphingolipid biosynthesis. We further showed that the fatty acid side-chains of glycosylceramides contain 20%–30% mmBCFAs and that this fraction is greatly diminished in the absence of mmBCFA biosynthesis. These results suggest a model in which a C17ISO-containing sphingolipid may mediate the regulatory functions of mmBCFAs and is negatively regulated by TAT-2 in intestinal cells. This work indicates a novel connection between a P-type ATPase and the critical regulatory function of a specific fatty acid

The Hamburg/SAO Survey for Emission--Line Galaxies. V. The Fifth List of 161 Galaxies

We present the fifth list with results of the Hamburg/SAO Survey for Emission-Line Galaxies (HSS therein, SAO -- Special Astrophysical Observatory, Russia). The list is a result of follow-up spectroscopy conducted with the 2.2m CAHA and 4m Kitt Peak telescopes in 1999. The data of this snap-shot spectroscopy survey confirmed 166 emission-line objects out of 209 observed candidates and allowed their quantitative spectral classification and redshift determination. We could classify 98 emission-line objects as BCG/HII galaxies or probable BCGs, 5 -- as QSOs, 3 - as Seyfert galaxies, 2 - as super-associations in subluminous spiral galaxies. 25 low-excitation objects were classified as starburst nuclei (SBN), 24 as dwarf amorphous nuclei starburst galaxies (DANS) and 3 as LINERs. Due to low signal-to-noise ratio we could not classify 6 ELGs. Furthermore, for another 4 galaxies we did not detect any significant emission lines. For 131 emission-line galaxies, the redshifts and/or line intensities are determined for the first time. For the remaining 30 previously known ELGs we give either improved data of the line intensities or some independent measurements.Comment: 18 pages, A&A in press. Figures A1-A17 (spectra) available in GIF-format. PS-file with Figures A1-A17 available via http://precise.sao.ru/Laboratory/Publications/2001/HSSV_figs.ps.g

Coordinate Regulation of Lipid Metabolism by Novel Nuclear Receptor Partnerships

Mammalian nuclear receptors broadly influence metabolic fitness and serve as popular targets for developing drugs to treat cardiovascular disease, obesity, and diabetes. However, the molecular mechanisms and regulatory pathways that govern lipid metabolism remain poorly understood. We previously found that the Caenorhabditis elegans nuclear hormone receptor NHR-49 regulates multiple genes in the fatty acid beta-oxidation and desaturation pathways. Here, we identify additional NHR-49 targets that include sphingolipid processing and lipid remodeling genes. We show that NHR-49 regulates distinct subsets of its target genes by partnering with at least two other distinct nuclear receptors. Gene expression profiles suggest that NHR-49 partners with NHR-66 to regulate sphingolipid and lipid remodeling genes and with NHR-80 to regulate genes involved in fatty acid desaturation. In addition, although we did not detect a direct physical interaction between NHR-49 and NHR-13, we demonstrate that NHR-13 also regulates genes involved in the desaturase pathway. Consistent with this, gene knockouts of these receptors display a host of phenotypes that reflect their gene expression profile. Our data suggest that NHR-80 and NHR-13's modulation of NHR-49 regulated fatty acid desaturase genes contribute to the shortened lifespan phenotype of nhr-49 deletion mutant animals. In addition, we observed that nhr-49 animals had significantly altered mitochondrial morphology and function, and that distinct aspects of this phenotype can be ascribed to defects in NHR-66– and NHR-80–mediated activities. Identification of NHR-49's binding partners facilitates a fine-scale dissection of its myriad regulatory roles in C. elegans. Our findings also provide further insights into the functions of the mammalian lipid-sensing nuclear receptors HNF4α and PPARα

How to use neural network and web technologies in modeling complex technical systems

This paper discusses the problem of integrating modern methods of forecasting and modeling complex technical objects into the learning process. As an example, the problem of solving a system of ordinary differential equations is considered, which has significant practical application. In particular, solving a system of differential equations can be an essential part of patents. The neural network method to solve this problem by using Matlab simulation software and visual modeling tool Simulink is considered. Efficient cloud-based solution to ordinary differential equations is presented

FINANCIAL AND ORGANIZATIONAL BASE OF TELEMEDICINE AS A DRIVER FOR THE DEVELOPMENT OF VOLUNTARY HEALTH INSURANCE IN RUSSIA

The publication examines the usage of telemedicine as a possible factor in the development of voluntary health insurance. We examine the current state of the market of online technologies in healthcare. Proposals for introducing telemedicine into service packages and individual programs under the voluntary health insurance agreement are made. Possible risks and difficulties associated with the use of this technology are determined.В публикации исследуется внедрение телемедицины как возможный фактор развития добровольного медицинского страхования. Рассматривается текущее состояние рынка онлайн-технологий в здравоохранении. Формируются предложения по внедрению телемедицины в пакеты услуг и отдельные программы в рамках договора добровольного медицинского страхования. Определяются возможные риски и сложности, связанные с использованием данной технологии