Meeting the challenges related to material issues in chemical industries

Reliable performance and profitability are two important requirements for any chemical industry. In order to achieve high level of reliability and excellent performance, several issues related to design, materials selection, fabrication, quality assurance, transport, storage, inputs from condition monitoring, failure analysis etc. have to be adequately addressed and implemented. Technology related to nondestructive testing and monitoring of the plant is also essential for precise identification of defect sites and to take appropriate remedial decision regarding repair, replacement or modification of process conditions. The interdisciplinary holistic approach enhances the life of critical engineering components in chemical plants. Further, understanding the failure modes of the components through the analysis of failed components throws light on the choice of appropriate preventive measures to be taken well in advance, to have a control over the overall health of the plant. The failure analysis also leads to better design modification and condition monitoring methodologies, for the next generation components and plants. At the Indira Gandhi Centre for Atomic Research (IGCAR), Kalpakkam, a unique combination of the expertise in design, materials selection, fabrication, NDT development, condition monitoring, life prediction and failure analysis exists to obtain desired results for achieving high levels of reliability and performance assessment of critical engineering components in chemical industries. Case studies related to design, materials selection and fabrication aspects of critical components in nuclear fuel reprocessing plants, NDT development and condition monitoring of various components of nuclear power plants, and important failure investigations on critical engineering components in chemical and allied industries are discussed in this paper. Future directions are identified and planned approaches are briefly described

GODAE systems in operation

During the last 15 years, operational oceanography systems have been developed in several countries around the world. These developments have been fostered primarily by the Global Ocean Data Assimilation Experiment (GODAE), which coordinated these activities, encouraged partnerships, and facilitated constructive competition. This multinational coordination has been very beneficial for the development of operational oceanography. Today, several systems provide routine, real-time ocean analysis, forecast, and reanalysis products. These systems are based on (1) state-of-the-art Ocean General Circulation Model (OGCM) configurations, either global or regional (basin-scale), with resolutions that range from coarse to eddy-resolving, and (2) data assimilation techniques ranging from analysis correction to advanced three- or four-dimensional variational schemes. These systems assimilate altimeter sea level anomalies, sea surface temperature data, and in situ profiles of temperature and salinity, including Argo data. Some systems have implemented downscaling capacities, which consist of embedding higher-resolution local systems in global and basin-scale models (through open boundary exchange of data), especially in coastal regions, where small scale-phenomena are important, and also increasing the spatial resolution for these regional/coastal systems to be able to resolve smaller scales (so-called downscaling). Others have implemented coupling with the atmosphere and/or sea ice. This paper provides a short review of these operational GODAE systems.Published76-914.6. Oceanografia operativa per la valutazione dei rischi in aree marineN/A or not JCRope

GODAE systems in operation

During the last 15 years, operational oceanography systems have emerged in several countries around the world. This emergence has been largely fostered by the GODAE experiment, during which each nation engaged in this activity have organised partnership and constructive competition. This trans-national coordination was very beneficial for the development of operational oceanography, leading to economies of scales and more targeted actions. Today, several systems provide routine real-time ocean analysis and forecast and/or reanalysis products. They are all based on (i) state-of-the-art primitive equation baroclinic Ocean General Circulation Model (OGCM) configurations, either global or regional (basin-scale), with resolutions that range from coarse to eddy resolving and (ii) data assimilation techniques whose complexity ranges from simple analysis correction to advanced 4D variational schemes. They assimilate altimeter sea level anomalies, remotely sensed SST such as GHRSST products and in situ profiles of T and S, including ARGO. Some systems have implemented downscaling capacities in specific regions of interest including shelf/coastal seas. Some also have implemented coupling with the atmosphere and/or the prognostic sea ice in polar regions. They are the GODAE system in operation. They are reviewed in this paper. The GODAE system discussed here include: (1) BLUElink OceanMAPS, (2) C-NOOFS, , (3) ECCO, (4) FOAM, (5) HYCOM/NCODA, (6) MERCATOR, (7) MFS, (8) MOVE/MRI.COM, (9) NLOM/NCOM, (10) NMEFC, (11) RTOFS and (12) TOPAZ.SubmittedNice, France3.11. Oceanografia Operativaope

GODAE systems in operation

During the last 15 years, operational oceanography systems have been developed in several countries around the world. These developments have been fostered primarily by the Global Ocean Data Assimilation Experiment (GODAE), which coordinated these activities, encouraged partnerships, and facilitated constructive competition. This multinational coordination has been very beneficial for the development of operational oceanography. Today, several systems provide routine, real-time ocean analysis, forecast, and reanalysis products. These systems are based on (1) state-of-the-art Ocean General Circulation Model (OGCM) configurations, either global or regional (basin-scale), with resolutions that range from coarse to eddy-resolving, and (2) data assimilation techniques ranging from analysis correction to advanced three- or four-dimensional variational schemes. These systems assimilate altimeter sea level anomalies, sea surface temperature data, and in situ profiles of temperature and salinity, including Argo data. Some systems have implemented downscaling capacities, which consist of embedding higher-resolution local systems in global and basin-scale models (through open boundary exchange of data), especially in coastal regions, where small scale-phenomena are important, and also increasing the spatial resolution for these regional/coastal systems to be able to resolve smaller scales (so-called downscaling). Others have implemented coupling with the atmosphere and/or sea ice. This paper provides a short review of these operational GODAE systems

GODAE systems in operation

During the last 15 years, operational oceanography systems have emerged in several countries around the world. This emergence has been largely fostered by the GODAE experiment, during which each nation engaged in this activity have organised partnership and constructive competition. This trans-national coordination was very beneficial for the development of operational oceanography, leading to economies of scales and more targeted actions. Today, several systems provide routine real-time ocean analysis and forecast and/or reanalysis products. They are all based on (i) state-of-the-art primitive equation baroclinic Ocean General Circulation Model (OGCM) configurations, either global or regional (basin-scale), with resolutions that range from coarse to eddy resolving and (ii) data assimilation techniques whose complexity ranges from simple analysis correction to advanced 4D variational schemes. They assimilate altimeter sea level anomalies, remotely sensed SST such as GHRSST products and in situ profiles of T and S, including ARGO. Some systems have implemented downscaling capacities in specific regions of interest including shelf/coastal seas. Some also have implemented coupling with the atmosphere and/or the prognostic sea ice in polar regions. They are the GODAE system in operation. They are reviewed in this paper. The GODAE system discussed here include: (1) BLUElink OceanMAPS, (2) C-NOOFS, , (3) ECCO, (4) FOAM, (5) HYCOM/NCODA, (6) MERCATOR, (7) MFS, (8) MOVE/MRI.COM, (9) NLOM/NCOM, (10) NMEFC, (11) RTOFS and (12) TOPAZ

Molecular epidemiology of Bordetella pertussis in the Philippines in 2012–2014

SummaryObjectivesThe present study was designed to determine the genotypes of circulating Bordetella pertussis in the Philippines by direct molecular typing of clinical specimens.MethodsNasopharyngeal swabs (NPSs) were collected from 50 children hospitalized with pertussis in three hospitals during 2012–2014. Multilocus variable-number tandem repeat analysis (MLVA) was performed on the DNA extracts from NPSs. B. pertussis virulence-associated allelic genes (ptxA, prn, and fim3) and the pertussis toxin promoter, ptxP, were also investigated by DNA sequence-based typing.ResultsTwenty-six DNA extracts yielded a complete MLVA profile, which were sorted into 10 MLVA types. MLVA type 34 (MT34), which is rare in Australia, Europe, Japan, and the USA, was the predominant strain (50%). Seven MTs (MT29, MT32, MT33, and MT283–286, total 42%) were single-locus variants of MT34, while two (MT141 and MT287, total 8%) were double-locus variants of MT34. All MTs had the combination of virulence-associated allelic genes, ptxP1–ptxA1–prn1–fim3A.ConclusionsThe B. pertussis population in the Philippines comprises genetically related strains. These strains are markedly different from those found in patients from other countries where acellular pertussis vaccines are used. The differences in vaccine types between these other countries and the Philippines, where the whole-cell vaccine is still used, may select for distinct populations of B. pertussis

B1 SOX Coordinate Cell Specification with Patterning and Morphogenesis in the Early Zebrafish Embryo

The B1 SOX transcription factors SOX1/2/3/19 have been implicated in various processes of early embryogenesis. However, their regulatory functions in stages from the blastula to early neurula remain largely unknown, primarily because loss-of-function studies have not been informative to date. In our present study, we systematically knocked down the B1 sox genes in zebrafish. Only the quadruple knockdown of the four B1 sox genes sox2/3/19a/19b resulted in very severe developmental abnormalities, confirming that the B1 sox genes are functionally redundant. We characterized the sox2/3/19a/19b quadruple knockdown embryos in detail by examining the changes in gene expression through in situ hybridization, RT–PCR, and microarray analyses. Importantly, these phenotypic analyses revealed that the B1 SOX proteins regulate the following distinct processes: (1) early dorsoventral patterning by controlling bmp2b/7; (2) gastrulation movements via the regulation of pcdh18a/18b and wnt11, a non-canonical Wnt ligand gene; (3) neural differentiation by regulating the Hes-class bHLH gene her3 and the proneural-class bHLH genes neurog1 (positively) and ascl1a (negatively), and regional transcription factor genes, e.g., hesx1, zic1, and rx3; and (4) neural patterning by regulating signaling pathway genes, cyp26a1 in RA signaling, oep in Nodal signaling, shh, and mdkb. Chromatin immunoprecipitation analysis of the her3, hesx1, neurog1, pcdh18a, and cyp26a1 genes further suggests a direct regulation of these genes by B1 SOX. We also found an interesting overlap between the early phenotypes of the B1 sox quadruple knockdown embryos and the maternal-zygotic spg embryos that are devoid of pou5f1 activity. These findings indicate that the B1 SOX proteins control a wide range of developmental regulators in the early embryo through partnering in part with Pou5f1 and possibly with other factors, and suggest that the B1 sox functions are central to coordinating cell fate specification with patterning and morphogenetic processes occurring in the early embryo

Exportin 4 Interacts with Sox9 through the HMG Box and Inhibits the DNA Binding of Sox9

Sox9 is a transcription factor that is required for tissue development in mammals. In general, such transcription factors require co-regulators for precise temporal and spatial control of the activation and inactivation of the numerous genes necessary for precise development during embryogenesis. Here we identify a new Sox9 co-regulator: Using affinity chromatography with immobilized Sox9 protein, we identified exportin 4 (Exp4) as an interacting protein of Sox9 in human cultured cells. Interaction between endogenous Exp4 and Sox9 proteins was confirmed in the human osteosarcoma U2OS cells by immunoprecipitation experiments using anti-Sox9 antibody. siRNA depletion of Exp4 enhanced transcription of Sox9 target genes in U2OS cells, but did not affect nuclear localization of Sox9. These results suggest that Exp4 regulates Sox9 activity in the nucleus. Furthermore we found that the HMG box of Sox9 was responsible for binding to Exp4, and the HMG box was required for suppression of Sox9-mediated transcription. This contrasts with the known Sox9 co-regulators which bind to its transcriptional activation domain. Chromatin immunoprecipitation analyses revealed that Exp4 prevents Sox9 binding to the enhancers of its target genes. These results demonstrate that Exp4 acts as a Sox9 co-regulator that directly regulates binding of Sox9 to its target genes