EFFECT OF CE3+ METAL IONS ON THE ANTIBACTERIAL AND ANTICANCER ACTIVITY OF ZINC OXIDE NANOPARTICLES PREPARED BY COPRECIPITATION METHOD

ABSTRACTObjective: This study was undertaken to know about the antibacterial and anticancer activity of synthesized zinc oxide (ZnO) nanoparticles (NPs).Methods: The ZnO NPs and different concentration of Ce3+ (0.05M, 0.10M, and 0.15M)-doped ZnO NPs were synthesized by coprecipitation method.The synthesized nanoparticles were analyzed by X-ray diffraction (XRD) and HRSEM. The antibacterial studies were performed against a set ofbacterial strains as Gram-positive bacteria (Streptococcus aureus and Streptococcus pneumonia) and Gram-negative (Escherichia coli, Pseudomonasaeruginosa, Proteus vulgaris, Klebsiella pneumonia, and Shigella dysenteriae) bacteria. The cytotoxic effect of ZnO and Ce-doped ZnO was analyzed incultured (A549) human lung cancer cell line.Result: The XRD studies showed the wurtzite structure of nanoparticles. HRSEM analysis showed the spherical shape of ZnO and Ce-doped ZnO. TheZn0.85Ce0.15O NPs possessed more antibacterial effect as compared to the other ZnO and Ce-doped ZnO NPs. The Zn0.90Ce0.10O NPs created the highestcytotoxicity activity. With respect to cell death, as low a concentration of 68±0.05 μg/ml of Zn0.90Ce0.10O NPs was good enough to cause loss of viabilityof 50% of the cell as compared to ZnO and Zn1-xCexO (x=0.05 and 0.15) NPs.Conclusion: Results from this work concluded that Zn0.85Ce0.15O and Zn0.90Ce0.10O NPs possess antibacterial and anticancer activity, respectively.Keywords: Zinc oxide nanoparticles, Coprecipitation method, Antibacterial activity and anticancer activity, Human lung cancer cell line

A Hospital - Based Observational Study on Clinical Profile of Ischemic and Haemorrhagic Stroke and Validation of Siriraj Stroke Score System

BACKGROUND: Ischemic and haemorrhagic stroke are the two major types of stroke, that contribute much to the neurological causes of morbidity and mortality. But their treatment modality differs. Hence it is essential to differentiate between infarct and haemorrhagic stroke. Siriraj stroke score helps in differentiating between these two conditions. In situations, where neuroimaging is not possible, Siriraj stroke score system can be used and prompt treatment can be initiated, since success of treatment depends on how fast the treatment has been initiated. AIMS AND OBJECTIVES: 1. To ascertain the clinical profile of ischemic and haemorrhagic stroke. 2. To determine the validity of Siriraj stroke score system in differentiating ischemic and haemorrhagic stroke. MATERIALS AND METHODS: All patients are subjected to thorough history taking and clinical examination and are subjected to neuroimaging by noncontrast CT brain and MRI brain. After getting informed consent from patient/ patients attenders, after removing metallic objects from body, patient is made to lie on CT examination table, lying flat on her/his back. CT brain will be taken and then MRI Brain. RESULT: There existed a significant correlation between Siriraj stroke score system and MRI Brain findings, which shows a significant validity for using Siriraj stroke score system as bedside test in differentiating ischemic from haemorrhagic stroke. CONCLUSION: ◈ As Siriraj stroke score has significant correlation with MRI brain, it can be used as bedside test for differentiating infarct from haemorrhagic stroke. ◈ It can be used in conditions where CT and MRI are not possible. ◈ Since hypertension and Diabetes mellitus has significant correlation with incidence of stroke, early diagnosis and treatment of stroke can reduce the incidence of stroke

ANTICANCER EFFECT OF METHANOLIC EXTRACT OF ANNONA SQUAMOSA ON MAMMARY CARCINOMA IN RATS WITH REFERENCE TO GLYCOPROTEIN COMPONENTS, LYSOSOMAL, AND MARKER ENZYMES

  Objectives: The aim is to evaluate the anticancer potential of Annona squamosa (AS) against 7, 12-dimethylbenz(a)anthracene (DMBA)-induced mammary carcinoma in rats.Methods: The tumor was induced in Sprague-Dawley rats by gastric intubation of 25 mg DMBA in 1 ml olive oil. After 3 months of induction period, the methanolic extract of AS at different doses of 100, 150, 200, 250, and 300 mg/kg body weight were administered orally a dose per day to mammary carcinoma-bearing rats for 45 days. The serum and tissue levels of glycoprotein components as well as the activities of marker enzymes and lysosomal enzymes were measured in DMBA-induced mammary carcinoma-bearing rats.Results: Administration of AS resulted in decrease in the levels of marker and lysosomal enzymes and also alterations in the body weight and tumor volume were also restored to near normalcy in a dose-dependent manner. The results of the present study indicate that AS has anticancer effect and it exhibits its potential effect at the dosage of 200 mg/kg body weight in mammary carcinoma-bearing rats.Conclusions: Based on the results obtained, it can be concluded that the methanol extract of AS possesses anticancer properties. Further study is needed to isolate the active principle of this extract responsible for anticancer activity to develop the future pharmaceuticals

IMPLEMENTATION OF MESSAGE AUTHENTICATION SCHEME WITH ELLIPTIC CURVE CRYPTOGRAPHY

ABSTRACT Transmission of private information over the public channels requires security or data protection against unauthorized access. Elliptic Curve Cryptography (ECC) is one of the efficient encryption technique can be used to secure the private data. High level security requirement of Restricted Services of Indian Regional Navigation Satellite System (IRNSS) to transmit the navigation data through wireless channel, can be achieved by ECC with minimum key size.ECC is based on Elliptic Curve Scalar Multiplication (ECSM) which is the process of multiplying a point on elliptic curve by a scalar value. The operations has been performed on National Institute of Standards and Technology (NIST) recommended elliptic curves over binary field

QCD Working Group Report

This is the report of the QCD working group at WHEPP 6. Discussions and work on heavy ion collisions, polarised scattering, and collider phenomenology are reported.Comment: Report of the QCD group at WHEPP-6, Chennai, January 2000. 7 page

RECENT TRENDS IN MEAT AND MEAT PRODUCTS PACKAGING -A REVIEW

ABSTRACT The aim of any packaging system for fresh muscle foods is to prevent or delay undesirable changes to the appearance, flavour, odour, and texture. Deterioration in these qualities can result in economic losses due to consumer rejection of the product. Therefore, a preservative packaging should ideally inhibit undesirable enzyme activities, but not interfere with, or inhibit, activities that are beneficial. The non-enzymatic reactions that affect the organoleptic qualities of raw meats are invariably undesirable, so these should preferably be slowed or prevented by a preservative packaging

Extraction process optimization of flavonoid and in vitro amylase inhibitory effect of purified quercetin derivative from Amorphophallus paeoniifolius tubers

544-556Amorphophallus paeoniifolius (Elephant foot yam) is a prominent tuberous plant utilized across several parts of India to treat various ailments such as a tumour, haemorrhage, microbial infections, cough, bronchitis, diabetes, anaemia, and hepato-gastro and cardiovascular diseases. In this context, the present study aims to optimize the extraction process of the flavonoid and to study the in vitro amylase inhibitory effect of purified flavonoid moiety. The Shake flask method with different extraction solvents was adopted to quantify the flavonoid content. Central composite design (CCD) based response surface methodology (RSM) was formulated to optimize the extraction process. Three-dimensional preparative chromatography (3D PTLC) was executed to purify the flavonoid content and high-resolution liquid chromatography-mass spectrometry (HRLC-MS) was adopted to predict the structure. 3,5-dinitrosalicylic acid (DNS) based spectrophotometry method was used to determine the amylase inhibitory property. All the analyses were subjected to standard statistical tests. The developed model for the extraction optimization process was found to be near significant (P = 0.242) with temperature as a significant variable (P = 0.029), and a 107-fold increase (71.11±0.5 mg/g tissue) of flavonoid content was recorded. A strong yellow colour spot (flavonoid fraction) was eluted using 3D PTLC technique and the molecule was identified as quercetin derivative (m/z 447) by the direct MS method. Significant amylase inhibition (36.1±2.1%) recorded by purified quercetin derivative has documented the utilization of A. paeoniifolius tubers as classical traditional medicine

Identifying the Neutrino mass Ordering with INO and NOvA

The relatively large value of $\theta_{13}$ established recently by the Daya Bay reactor experiment opens the possibility to determine the neutrino mass ordering with experiments currently under construction. We investigate synergies between the NOvA long-baseline accelerator experiment with atmospheric neutrino data from the India-based Neutrino Observatory (INO). We identify the requirements on energy and direction reconstruction and detector mass for INO necessary for a significant sensitivity. If neutrino energy and direction reconstruction at the level of 10% and 10 degree can be achieved by INO a determination of the neutrino mass ordering seems possible around 2020.Comment: 18 pages, 8 figures, minor improvements and clarifications, new panel in fig. 7, version to appear in JHEP, typo in eq. 4 correcte

The electron thermal propagator at p>>T: An entire function of p_{0}

The retarded electron propagator S_{R}(p_{0},p) at high momentum p>>T was shown by Blaizot and Iancu to be an entire function of complex p_{0}. In this paper a specific form for S_{R}(p_{0},p) is obtained and checked by showing that its temporal Fourier transform S_{R}(t, p) has the correct behavior at large t. Potential infrared and collinear divergences from the emission of soft photons do not occur.Comment: 8 page

Neutrinos from Stellar Collapse: Effects of flavour mixing

We study the effect of non-vanishing masses and mixings among neutrino flavours on the detection of neutrinos from stellar collapse by a water Cerenkov detector. We consider a realistic framework in which there are three neutrino flavours whose mass squared differences and mixings are constrained by the present understanding of solar and atmospheric neutrinos. We also include the effects of high dense matter within the supernova core. We find that the number of events due to the dominant process involving electron-antineutrinos may change dramatically for some allowed mixing parameters. Furthermore, contributions from charged-current scattering off oxygen atoms in the detector can be considerably enhanced due to flavour mixing; such events have a distinct experimental signature since they are backward-peaked. Hence, mixing has a non-trivial effect on the signature of neutrinos (and antineutrinos) from stellar collapse.Comment: 22 pages Latex file, with 6 postscript figures, minor changes made in tex