Rare Animals, Poor People, and Big Agencies: A Perspective on Biological Conservation and Rural Development in the Himalaya

CT In earlier issues of Mountain Research and Development (Vol. 5, Nos. 2 and 3), Thompson and Warburton developed an institutional approach to development in the Himalayan region: an approach that, in treating the institutions and the perceptions they generate as the facts, largely dissolved away the physical constraints in a sea of uncertainty. In this article, we try to complete the exploratory circle by bringing nature-the physical constraints-back into our cultural picture. All institutions, sooner or later, bump against these constraints; the local farmers sooner, the international agencies later. Learning-readjustments in systems of knowledge - then takes place. Nature, in effect, forces the different systems of knowledge that are promoted by different institutions into conversation with one another. The present challenge is to convert that conversation from monologue to dialogu

The effect of self-efficacy on behavior and weight in a behavioral weight-loss intervention.

To determine whether eating self-efficacy and physical activity self-efficacy are predictive of dietary intake, physical activity, and weight change within a behavioral weight loss intervention, and whether dietary intake and physical activity mediate relationships between self-efficacy and weight change

Randomized trial comparing group size of periodic in-person sessions in a remotely delivered weight loss intervention

Abstract Background Few randomized studies have examined differential effects of group size in behavioral weight control, especially in hybrid programs that include Internet treatment approaches. Methods Randomized controlled trial (n = 195) comparing a 4 month hybrid internet weight loss program coupled with monthly face to face groups of 100 persons (Large Group, LG; 1 group) or to the same approach with monthly groups of 20 persons (Small Group, SG; 4 groups). Repeated-measures mixed-model analysis with age and race as covariates were used to estimate primary (weight) and secondary outcomes, and to test group differences in change over time. Results The sample was 46.3 years old ±10.4, 90.3% female, and 51.9% non-white, with BMI 37.9 ± 8.4 kg/m2. Participants in the LG were more likely to return for the 4-month assessment visit than those in the SG (p = 0.04). Participants randomized to both the LG and SG conditions experienced significant WL over time (no between group difference: −4.1 kg and −3.7 kg, respectively) and weight loss was positively associated with attendance at monthly meetings and logins to the website. Satisfaction with the program was high and similar in both groups (94.4% reported that they were “satisfied” or “very satisfied”). Conclusions Using a hybrid approach of in-person and online weight loss interventions may be an effective way to reach larger and more diverse populations. Delivering the face to face component of the intervention in groups larger than those traditionally delivered (20–25 people) could increase the cost-effectiveness of group-based behavioral weight loss interventions. Clinical trials registration number NCT01615471 . Registered June 6, 2012. Registered retrospectively

Acculturation Influences Postpartum Eating, Activity, and Weight Retention in Low-Income Hispanic Women

Background: Low-income Hispanic women experience elevated rates of high postpartum weight retention (PPWR), which is an independent risk factor for lifetime obesity. Sociocultural factors might play an important role among Hispanic women; however, very few studies have examined this association. Objective: The purpose of our study was to examine the associations between acculturation and maternal diet, physical activity, and PPWR. Design: This is a cross-sectional study of baseline data from 282 Hispanic women participating in the FitMoms/Mamás Activas study, a randomized controlled trial examining the impact of primarily an internet-based weight control program, in reducing PPWR among low-income women. We performed multivariable linear regression to examine the association of acculturation with diet quality, physical activity, and PPWR at study entry. Results: A total of 213 (76%) women had acculturation scores reflecting Mexican orientation or bicultural orientation, whereas 69 (24%) had scores that represented assimilation to Anglo culture. Women who were more acculturated had lower intakes of fruits and vegetables, lower HEI scores, and lower physical activity levels than women who were less acculturated (p < 0.05). We found an association between acculturation and PPWR in that for every 1-unit increase in acculturation score, PPWR increased, on average, by 0.80 kg. Conclusion: Higher acculturation was associated with poorer diet and physical activity behaviors and greater PPWR

Targeted knock-down of miR21 primary transcripts using snoMEN vectors induces apoptosis in human cancer cell lines

We have previously reported an antisense technology, 'snoMEN vectors', for targeted knock-down of protein coding mRNAs using human snoRNAs manipulated to contain short regions of sequence complementarity with the mRNA target. Here we characterise the use of snoMEN vectors to target the knock-down of micro RNA primary transcripts. We document the specific knock-down of miR21 in HeLa cells using plasmid vectors expressing miR21-targeted snoMEN RNAs and show this induces apoptosis. Knock-down is dependent on the presence of complementary sequences in the snoMEN vector and the induction of apoptosis can be suppressed by over-expression of miR21. Furthermore, we have also developed lentiviral vectors for delivery of snoMEN RNAs and show this increases the efficiency of vector transduction in many human cell lines that are difficult to transfect with plasmid vectors. Transduction of lentiviral vectors expressing snoMEN targeted to pri-miR21 induces apoptosis in human lung adenocarcinoma cells, which express high levels of miR21, but not in human primary cells. We show that snoMEN-mediated suppression of miRNA expression is prevented by siRNA knock-down of Ago2, but not by knock-down of Ago1 or Upf1. snoMEN RNAs colocalise with Ago2 in cell nuclei and nucleoli and can be co-immunoprecipitated from nuclear extracts by antibodies specific for Ago2