42 research outputs found
Antiulcerative properties of crude polyphenols and juice of apple, and Chinese quince extracts
ArticleFOOD CHEMISTRY. 108(2): 488-495 (2008)journal articl
Consistent lack of CD34-positive stromal cells in the stroma of malignant breast lesions
To examine the distribution of CD34-positive
and ASMA-positive stromal cells in various breast
lesions, we performed immunohistochemical assays
(using a streptavidin-biotin immunoperoxidase
technique) of tissue specimens, obtained by excisional
biopsy and partial or total mastectomy, from 62 patients
with breast lesions. Specimens were obtained from 64
lesions as follows: fibrocystic disease (n=12), intraductal
papilloma (n=4), fibroadenoma (n=17), invasive lobular
carcinoma (n=6), invasive ductal carcinoma (n=20) and
invasive micropapillary carcinoma (n=5). In normal
breast tissue (controls), CD34-positive spindle cells were
abundant in the intralobular stroma, but no ASMApositive
stromal cells were identified except
myoepithelial cells. Small to large numbers of CD34-
positive cells were observed in the stroma of 29 of 33
benign diseases. In all invasive carcinomas (lobular,
ductal and micropapillary), no CD34-positive stromal
cells were observed in the stroma. In the stroma of
benign lesions, the number of ASMA-positive stromal
cells was various, but the stroma of all invasive breast
cancers contained ASMA-positive stromal cells. The
present results indicate that disappearance of CD34-
positive stromal cells consistently occurs in the stroma
of invasive carcinoma of the breast, irrespective of
histological type and may be associated with the
presence of ASMA-positive stromal cells
The distribution of myofibroblasts and CD34-positive stromal cells in normal renal pelvis and ureter and their cancers
In this article, we examined the distribution
of myofibroblasts and CD34-positive stromal cells in
normal renal pelvis and ureter and their cancers using
immunohistochemistry. Eighteen tumors and normal
tissues apart from the main tumor were examined. In the
wall of normal renal pelvis and ureter, no myofibroblasts
were observed through all layers, but CD34-positive
stromal cells were observed in the deep area of lamina
propria, muscular layer and adventitia. In the stroma of
renal pelvic and ureteral cancers, myofibroblasts were
distributed in fifteen tumors and were absent in three
tumors. All three tumors containing no myofibroblasts in
the stroma were non-invasive type and all invasive
cancers contained myofibroblasts in the stroma. CD34-
positive stromal cells were consistently absent in the
stroma of cancers, irrespective of the invasiveness.
Finally, myofibroblasts are major stromal components in
renal pelvic and ureteral cancers, particularly in invasive
cancers, and CD34-positive stromal cells are
consistently absent or lost in the stroma of their cancers.
These findings suggest that the invasion of renal pelvic
and ureteral cancers may cause the phenotypic change of
stromal cells
The appearance of myofibroblasts and the disappearance of CD34-positive stromal cells in the area adjacent to xanthogranulomatous foci of chronic cholecystitis
We investigated the distribution of
myofibroblasts and CD34-positive stromal cells in
normal gallbladder and its pathological conditions
(cholecystitis, n=25) using immunohistochemistry and in
situ hybridization. In the wall of normal gallbladder,
myofibroblasts were generally absent from all layers, but
many CD34-positive stromal cells were observed in the
connective tissue layer. In chronic cholecystitis with
mild perimuscular fibrosis, a small to moderate number
of myofibroblasts appeared in the mucosal layer. In
chronic cholecystitis with marked perimuscular fibrosis,
a small to large number of myofibroblasts appeared
predominantly in the connective tissue layer, whereas
the number of CD34-positive stromal cells decreased at
the same location, although the number of
myofibroblasts increased. In chronic cholecystitis with
xanthogranulomatous foci, a small to large number of
myofibroblasts were observed in the periphery of the
xanthogranulomatous reaction and adjacent area. In
contrast, CD34-positive stromal cells were completely
absent or were limited to the area just around the
xanthogranulomatous reaction. Induction of collagen
type I and III mRNA was predominantly observed in the
cytoplasm of myofibroblasts associated with the marked
fibrosis, which consisted primarily of mature collagen
fibers, and in the cytoplasm of myofibroblasts around
the xanthogranulomatous reaction, respectively. Finally,
myofibroblasts were observed in all subtypes. The
increased number of myofibroblasts was most prominent
in the connective tissue layer of chronic cholecystitis
with marked perimuscular fibrosis or in the area adjacent
to xanthogranulomatous foci of chronic cholecystitis.
Under these conditions, CD34-positive stromal cells
tended to disappear from the connective tissue layer,
which exhibited an increase in myofibroblasts
Non-extractable Procyanidins and Lignin are Important Factors in the Bile Acid Binding and Radical Scavenging Properties of Cell Wall Material in some Fruits
The cell wall components and the food functions of alcohol-insoluble solids (AIS) of Chinese quince, quince, hawthorn, apple, pear and blueberry fruits were analyzed. Chinese quince contained characteristically high contents of cellulose, lignin, and non-extractable procyanidins (NEPCs). On the other hand, the quince AIS contained the highest proportion of NEPCs, the highest mean degree of polymerization (mDP), the strongest radical scavenging activity, and strong bile acid binding activity. In fruit AIS, the lignin and NEPC contents both showed positive correlations with the bile acid binding and radical scavenging activities. The value for mDP x NEPC content was a good index for the radical scavenging activity. The results suggest that highly polymerized NEPCs and lignin are important factors of cell wall components of fruits to having a high functionality, and Chinese quince and quince are interesting fruits from this view point
Ordinary and supernumerary resonant scattering of low energy electrons from the BiCu 2
Electron intensity versus energy curves from the ordered substitutional BiCu2(111)-surface alloy, obtained with low energy electron microscopy (LEEM), show distinct unexpected intensity dips under normal incidence conditions. The dips above 10 eV are found to be characteristic of ordinary resonant scattering of electrons. Their positions represent resonant scattering into image potential states, involving scattering along G 10 and G 11 reciprocal lattice vectors. A detailed analysis of additional specular intensity dips at energies below 10 eV demonstrates that these originate from supernumerary resonant scattering, now also along lower symmetry directions, that should be forbidden in this energy range for the perfect crystal. LEEM is highly sensitive to detect these often neglected features which, if not properly disentangled or discarded, hamper its potential to extract the rich information about atomic positions and unoccupied electronic states encoded in very low energy electron scattering