13,462 research outputs found
RAD6-RAD18-RAD5-pathway-dependent tolerance to chronic low-dose ultraviolet light
In nature, organisms are exposed to chronic low- dose ultraviolet light ( CLUV) as opposed to the acute high doses common to laboratory experiments. Analysis of the cellular response to acute high-dose exposure has delineated the importance of direct DNA repair by the nucleotide excision repair pathway(1) and for checkpoint-induced cell cycle arrest in promoting cell survival(2). Here we examine the response of yeast cells to CLUV and identify a key role for the RAD6-RAD18-RAD5 error- free postreplication repair (RAD6 error-free PRR) pathway(3,4) in promoting cell growth and survival. We show that loss of the RAD6 error- free PRR pathway results in DNA-damage-checkpoint- induced G2 arrest in CLUV-exposed cells, whereas wild-type and nucleotide-excision-repair-deficient cells are largely unaffected. Cell cycle arrest in the absence of the RAD6 error- free PRR pathway was not caused by a repair defect or by the accumulation of ultraviolet-induced photoproducts. Notably, we observed increased replication protein A (RPA) and Rad52 - yellow fluorescent protein foci(5) in the CLUV- exposed rad18 Delta cells and demonstrated that Rad52- mediated homologous recombination is required for the viability of the rad18 Delta cells after release from CLUV- induced G2 arrest. These and other data presented suggest that, in response to environmental levels of ultraviolet exposure, the RAD6 error- free PRR pathway promotes replication of damaged templates without the generation of extensive single- stranded DNA regions. Thus, the error- free PRR pathway is specifically important during chronic low- dose ultraviolet exposure to prevent counter- productive DNA checkpoint activation and allow cells to proliferate normally
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An atlas of CO2 storage potential in the nearshore waters of the Texas coast – American Recovery and Reinvestment Act – “Gulf of Mexico Miocene CO2 site characterization mega-transect”
Bureau of Economic Geolog
Stability criterion for self-similar solutions with a scalar field and those with a stiff fluid in general relativity
A stability criterion is derived in general relativity for self-similar
solutions with a scalar field and those with a stiff fluid, which is a perfect
fluid with the equation of state . A wide class of self-similar
solutions turn out to be unstable against kink mode perturbation. According to
the criterion, the Evans-Coleman stiff-fluid solution is unstable and cannot be
a critical solution for the spherical collapse of a stiff fluid if we allow
sufficiently small discontinuity in the density gradient field in the initial
data sets. The self-similar scalar-field solution, which was recently found
numerically by Brady {\it et al.} (2002 {\it Class. Quantum. Grav.} {\bf 19}
6359), is also unstable. Both the flat Friedmann universe with a scalar field
and that with a stiff fluid suffer from kink instability at the particle
horizon scale.Comment: 15 pages, accepted for publication in Classical and Quantum Gravity,
typos correcte
Convergence to a self-similar solution in general relativistic gravitational collapse
We study the spherical collapse of a perfect fluid with an equation of state
by full general relativistic numerical simulations. For 0, it has been known that there exists a general relativistic counterpart
of the Larson-Penston self-similar Newtonian solution. The numerical
simulations strongly suggest that, in the neighborhood of the center, generic
collapse converges to this solution in an approach to a singularity and that
self-similar solutions other than this solution, including a ``critical
solution'' in the black hole critical behavior, are relevant only when the
parameters which parametrize initial data are fine-tuned. This result is
supported by a mode analysis on the pertinent self-similar solutions. Since a
naked singularity forms in the general relativistic Larson-Penston solution for
0, this will be the most serious known counterexample against
cosmic censorship. It also provides strong evidence for the self-similarity
hypothesis in general relativistic gravitational collapse. The direct
consequence is that critical phenomena will be observed in the collapse of
isothermal gas in Newton gravity, and the critical exponent will be
given by , though the order parameter cannot be the black
hole mass.Comment: 22 pages, 15 figures, accepted for publication in Physical Review D,
reference added, typos correcte
Primordial black holes as a tool for constraining non-Gaussianity
Primordial Black Holes (PBH's) can form in the early Universe from the
collapse of large density fluctuations. Tight observational limits on their
abundance constrain the amplitude of the primordial fluctuations on very small
scales which can not otherwise be constrained, with PBH's only forming from the
extremely rare large fluctuations. The number of PBH's formed is therefore
sensitive to small changes in the shape of the tail of the fluctuation
distribution, which itself depends on the amount of non-Gaussianity present. We
study, for the first time, how quadratic and cubic local non-Gaussianity of
arbitrary size (parameterised by f_nl and g_nl respectively) affects the PBH
abundance and the resulting constraints on the amplitude of the fluctuations on
very small scales. Intriguingly we find that even non-linearity parameters of
order unity have a significant impact on the PBH abundance. The sign of the
non-Gaussianity is particularly important, with the constraint on the allowed
fluctuation amplitude tightening by an order of magnitude as f_nl changes from
just -0.5 to 0.5. We find that if PBH's are observed in the future, then
regardless of the amplitude of the fluctuations, non-negligible negative f_nl
would be ruled out. Finally we show that g_nl can have an even larger effect on
the number of PBH's formed than f_nl.Comment: 9 pages, 5 figures, v2: version to appear in Phys. Rev. D with minor
changes, v3: typos corrected (including factor of 1/2 in erfc prefactor), no
changes to result
African Water: Supporting African involvement in the EU Framework Programme.
Water researchers in developing countries have yet to take full advantage of the funding and collaborative research opportunities presented by the EU Framework Programme. There are a variety of reasons for this, such as insufficient information and a lack of previous experience. The African Water initiative aims to increase the involvement of African water researchers through a range of activities including communication and dissemination, capacity building and development, and complementary initiatives. The project has demonstrated that there is a demand for such sector-specific support activities. However, African Water is a small component of a much larger process of partnership between the developed and the less-developed countries of the world, involving many different European and African organisations working across political, institutional and technical domains, and complementing the wide range of actions already being undertaken
Uniqueness of Self-Similar Asymptotically Friedmann-Robertson-Walker Spacetime in Brans-Dicke theory
We investigate spherically symmetric self-similar solutions in Brans-Dicke
theory. Assuming a perfect fluid with the equation of state , we show that there are no non-trivial solutions which approach
asymptotically to the flat Friedmann-Robertson-Walker spacetime if the energy
density is positive. This result suggests that primordial black holes in
Brans-Dicke theory cannot grow at the same rate as the size of the cosmological
particle horizon.Comment: Revised version, 4 pages, no figures, Revtex, accepted for
publication in Physical Review
Multifocal High-Grade Pancreatic Precursor Lesions: A Case Series and Management Recommendations
Background: The risk of developing invasive cancer in the remnant pancreas after resection of multifocal high-grade pancreatic precursor lesions is not well known. We report three patients who were followed up after resection of multifocal high-grade pancreatic intraepithelial neoplasia (PanIN)-3 or intraductal papillary mucinous neoplasia (IPMN), two of whom eventually developed invasive carcinoma. Presentation: 1) 68-year-old woman who had a laparoscopic distal pancreatectomy for multifocal mixed-type IPMN, identified as high-grade on final pathology, with negative surgical margins. During semiannual monitoring, eight years from the first surgery, the patient developed suspicious features prompting surgical resection of the body with final pathology revealing invasive ductal adenocarcinoma in the setting of IPMN. 2) 48-year-old woman who had a distal pancreatectomy for severe acute/chronic symptomatic pancreatitis, with final pathology revealing multifocal high-grade PanIN-3, with negative surgical margins. Despite semiannual monitoring, two years from the first surgery, the patient developed pancreatic adenocarcinoma with liver metastasis. 3) 55-year-old woman who had a Whipple procedure for symptomatic chronic pancreatitis, with multifocal PanIN-3 on final pathology. The patient underwent completion pancreatectomy due to symptomatology and her high-risk profile, with final pathology confirming multifocal PanIN-3. Conclusion: Multifocal high-grade dysplastic lesions of the pancreas might benefit from surgical resection
Homothetic Self-Similar Solutions of the Three-Dimensional Brans-Dicke Gravity
All homothetic self-similar solutions of the Brans-Dicke scalar field in
three-dimensional spacetime with circular symmetry are found in closed form.Comment: latex, five pages, without figur
Bound and resonance states of the nonlinear Schroedinger equation in simple model systems
The stationary nonlinear Schroedinger equation, or Gross-Pitaevskii equation,
is studied for the cases of a single delta potential and a delta-shell
potential. These model systems allow analytical solutions, and thus provide
useful insight into the features of stationary bound, scattering and resonance
states of the nonlinear Schroedinger equation. For the single delta potential,
the influence of the potential strength and the nonlinearity is studied as well
as the transition from bound to scattering states. Furthermore, the properties
of resonance states for a repulsive delta-shell potential are discussed.Comment: 19 pages, 10 figure
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