Planetary Spectroscopy

The goal of this task is to acquire physical data on the atmospheres of the outer planets and Titan by means of ground-based spectroscopy, spectrophotometry, and spectral imaging at visible to near-infrared wavelengths (approximately 0.3 to 2.5 micrometer). These data constrain physical parameters which characterize properties and distribution of aerosols in the atmospheres of these bodies. Reduced spectral imaging of Neptune was accomplished. The data were analyzed in several ways. Direct inspection of images reveals the distribution of discrete clouds in the atmosphere, which indicate that the global distribution of clouds has changed since earlier imaging. Disk-integrated photometry obtained from the images demonstrates that the diurnal variability at methane-band wavelengths is caused by the presence of discrete clouds; short-term variability is also seen in the rotational light curve, providing evidence for modification of cloud structure on the planet. The center-to-limb brightness profiles of the equatorial region of Neptune were analyzed, which provided constraints on the location, albedos, and optical depths of aerosol scattering layers in the troposphere and lower stratosphere

Near perihelion observations of Comet Halley from Shuttle orbiter

Intercept missions and the space telescope will return unique data on Comet Halley, but will leave important gaps in the observational coverage of the comet's activity, especially around the time of perihelion passage. A small package of instruments, which could be scheduled to fly on several shuttle missions, would be an effective means of extending observational coverage of Comet Halley to include the critical part of the apparition near perihelion passage. This approach would certainly be "second best" to a dedicated orbital comet observatory. However, it would be feasible in the sense that payload space appears to be available for at least two flights during the apparition, and instruments exist, or could be modified, or are being developed, which could be integrated into a package of the required size, and which would return useful physical data on the comet

The ortho-para H2 distribution on Uranus: Constraints from the collision-induced 3-0 dipole band and 4-0 S(0) and S(1) quadrupole line profiles

Recent high quality spectral observations have allowed the derivation of constraints on the atmospheric structure of Uranus. The present analysis, which is based on the detailed modeling of a broadband geometric albedo spectrum and high resolution observations of the H2 4-0 quadrupole and 6818.9-A CH4 features, yields (1) a family of models which parameterize an upper tropospheric haze layer, (2) a lower, optically infinite cloud at a given pressure level, (3) the cloud-level methane molar fraction, and (4) the mean ortho/para ratio in the visible atmosphere. The single scattering albedo of atmospheric aerosols exhibits a steep darkening between 5890 and 6040 A

NASA Outer Solar System Exploration

A viewgraph presentation on NASA's Outer Solar System Exploration is shown. The topics include: 1) Completed Missions; 2) Operating Missions; 3) Missions in Development; and 4) Future Missions

Infrared observations of planetary atmospheres

The goal of this research in to obtain infrared data on planetary atmospheres which provide information on several aspects of structure and composition. Observations include direct mission real-time support as well as baseline monitoring preceding mission encounters. Besides providing a broader information context for spacecraft experiment data analysis, observations will provide the quantitative data base required for designing optimum remote sensing sequences and evaluating competing science priorities. In the past year, thermal images of Jupiter and Saturn were made near their oppositions in order to monitor long-term changes in their atmospheres. Infrared images of the Jovian polar stratospheric hot spots were made with IUE observations of auroral emissions. An exploratory 5-micrometer spectrum of Uranus was reduced and accepted for publication. An analysis of time-variability of temperature and cloud properties of the Jovian atomsphere was made. Development of geometric reduction programs for imaging data was initiated for the sun workstation. Near-infrared imaging observations of Jupiter were reduced and a preliminary analysis of cloud properties made. The first images of the full disk of Jupiter with a near-infrared array camera were acquired. Narrow-band (10/cm) images of Jupiter and Saturn were obtained with acousto-optical filters

The role of integrins in Drosophila egg chamber morphogenesis

A Drosophila egg chamber is comprised of a germline cyst surrounded by a tightly-organised epithelial monolayer, the follicular epithelium (FE). Loss of integrin function from the FE disrupts epithelial organisation at egg chamber termini, but the cause of this phenotype remains unclear. Here we show that the -integrin Myospheroid (Mys) is only required during early oogenesis when the pre-follicle cells form the FE. mys mutants disrupt both the formation of a monolayered epithelium at egg chamber termini and the morphogenesis of the stalk between adjacent egg chambers, which develops through the intercalation of two rows of cells into a single-cell wide stalk. Secondary epithelia, like the FE, have been proposed to require adhesion to the basement membrane to polarise. However, Mys is not required for pre-follicle cell polarisation, as both follicle and stalk cells localise polarity factors correctly, despite being mispositioned. Instead, loss of integrins causes pre-follicle cells to basally constrict, detach from the basement membrane and become internalised. Thus, integrin function is dispensable for pre-follicle cell polarity but is required to maintain cellular organisation and cell shape during morphogenesis.This work was supported by a Wellcome Trust Principal Fellowship to D.St J. (080007, 207496) and by centre grant support from the Wellcome Trust (092096, 203144) and Cancer Research UK (A14492, A24823). H.E.L. was supported by a Herchel Smith Studentship, University of Cambridge. D.T.B. was supported by a Marie Curie Fellowship (funded by the European Commission) and the Wellcome Trust

Spindle orientation: a question of complex positioning.

The direction in which a cell divides is determined by the orientation of its mitotic spindle at metaphase. Spindle orientation is therefore important for a wide range of developmental processes, ranging from germline stem cell division to epithelial tissue homeostasis and regeneration. In multiple cell types in multiple animals, spindle orientation is controlled by a conserved biological machine that mediates a pulling force on astral microtubules. Restricting the localization of this machine to only specific regions of the cortex can thus determine how the mitotic spindle is oriented. As we review here, recent findings based on studies in tunicate, worm, fly and vertebrate cells have revealed that the mechanisms for mediating this restriction are surprisingly diverse.The Wellcome Trus

Tohoku-Hiroshima-Nagoya planetary spectra library: A method for characterizing planets in the visible to near infrared

There has not been a comprehensive framework for comparing spectral data from different planets.Such a framework is needed for the study of extrasolar planets and objects within the solar system. We have undertaken observations to compile a library of planet spectra for all planets, some moons, and some dwarf planets in the solar system to study their general spectroscopic and photometric natures. During May and November of 2008, we acquired spectra for the planets using TRISPEC, which is capable of simultaneous three-band spectroscopy in a wide wavelength range of 0.45 - 2.5 microns with low resolving power (lambda-over-Delta-lambda is 140 - 360). Patterns emerge from comparing the spectra. Analyzing their general spectroscopic and photometric natures, we show that it is possible to distinguish between gas planets, soil planets and ice planets. These methods can be applied to extrasolar observations using low resolution spectrography or broad-band filters. The present planet spectral library is the first library to contain observational spectra for all of the solar system planets, based on simultaneous observations in visible and near infrared wavelengths. This library will be a useful reference for analyzing extrasolar planet spectra, and for calibrating planetary data sets.Comment: 11 pages, 6 figures, Accepted on 28/08/2009 to appear in Section 10. Planets and planetary systems of Astronomy and Astrophysic

Drosophila MUS312 and the Vertebrate Ortholog BTBD12 Interact with DNA Structure-Specific Endonucleases in DNA Repair and Recombination

DNA recombination and repair pathways require structure-specific endonucleases to process DNA structures that include forks, flaps, and Holliday junctions. Previously, we determined that the Drosophila MEI-9-ERCC1 endonuclease interacts with the novel MUS312 protein to produce meiotic crossovers, and that MUS312 has a MEI-9-independent role in interstrand crosslink (ICL) repair. The importance of MUS312 to pathways crucial for maintaining genomic stability in Drosophila prompted us to search for orthologs in other organisms. Based on sequence, expression pattern, conserved protein-protein interactions, and ICL repair function, we determined that the mammalian ortholog of MUS312 is BTBD12. Orthology between these proteins and S. cerevisiae Slx4 helped identify a conserved interaction with a second structure-specific endonuclease, SLX1. Genetic and biochemical evidence described here and in related papers suggest that MUS312 and BTBD12 direct Holliday junction resolution by at least two distinct endonucleases in different recombination and repair contexts

Global functional analysis of nucleophosmin in Taxol response, cancer, chromatin regulation, and ribosomal DNA transcription

Analysis of lung cancer response to chemotherapeutic agents showed the accumulation of a Taxol-induced protein that reacted with an anti-phospho-MEK1/2 antibody. Mass spectroscopy identified the protein as nucleophosmin/B23 (NPM), a multifunctional protein with diverse roles: ribosome biosynthesis, p53 regulation, nuclear-cytoplasmic shuttling, and centrosome duplication. Our work demonstrates that following cellular exposure to mitosis-arresting agents NPM is phosphorylated and its chromatographic property is altered, suggesting changes in function during mitosis. To determine the functional relevance of NPM, its expression in tumor cells was reduced by siRNA. Cells with reduced NPM were treated with Taxol followed by microarray profiling accompanied by gene/protein pathway analyses. These studies demonstrate several expected and unexpected consequences of NPM depletion. The predominant downstream effectors of NPM are genes involved in cell proliferation, cancer, and the cell cycle. In congruence with its role in cancer, NPM is over-expressed in primary malignant lung cancer tissues. We also demonstrate a role for NPM in the expression of genes encoding SET (TAF1β) and the histone methylase SET8. Additionally, we show that NPM is required for a previously unobserved G2/M upregulation of TAF1A, which encodes the rDNA transcription factor TAFI48. These results demonstrate multi-faceted functions of NPM that can affect cancer cells