Biomass yield and fodder quality of Napier grass (Pennisetum purpureum) as affected by Pigeon pea (Cajanus cajan) intercrop and planting distance

The study sought to investigate the effect of two planting distances and Pigeon Pea (Cajanus cajan) intercrop on the biomass yield, chemical composition and in vitro organic matter digestibility (IVOMD) of Napier grass (Pennisetum purpureum). The treatments were laid in a 2x2 factorial RCBD. The factors were two planting distances (0.5x0.5 and 0.5x1m) and two levels of intercrop (Pigeon Pea intercrop and sole Napier grass). At 90-days, the grasses were harvested and biomass yield measured. The fodder was separated into leaves and stems for chemical and digestibility analysis in 2x2x2 factorial in CRD. The interaction at all levels was not significant for all the parameters. There was a significant main effect of planting distance, intercrop and botanical fraction on biomass yield, plant height, tiller number and fodder quality. Whilst biomass yield was higher (P=0.02) in the 0.5x0.5m, no significant difference was recorded in the effect of the intercrop. Whilst intercrop affected (P<0.05) IVOMD and ME, botanical fraction significantly affected DM, CP and ME. The leaf fraction had more (P<0.05) DM, CP and ME than the stem fraction. The study revealed that close planting distance enhanced biomass yield whilst Pigeon Pea intercrop and botanical fraction affected fodder quality of Napier grass

Effect of rumen content to water ratio in biogas production

Biogas technology is widely used as a better alternative in the handling of waste in many societies in the world. This study was conducted to investigate the effect of rumen content to water ratio in biogas production. An experiment was carried out using 75 litre capacity locally made anaerobic digester. Rumen contents were formulated in three proportions designated as T1, T2 and T3. T1 contained 30kg of rumen contents and 30 litres of tap water; T2 contained 20kg of rumen contents and 40 litres of tap water; and T3 contained 40kg of rumen contents and 20 litres of tap water. Each proportion was duplicated and the whole experiment was triplicated for 20 days each. The pH, substrate, ambient temperatures and the biogas produced were recorded in two days’ interval up to the 20 days. The result obtained from the study showed that there were significant differences (P < 0.05) in the average pH and the biogas produced. The pH of T2 (6.97) was significantly higher than that of T1 (6.80) and T3 (6.81). The biogas produced by T3 was significantly higher than that of T1 and T2. T3 produced the highest average volume of 16.70m3 biogas per kilogram of rumen content and the highest percentage (56.4%) of methane. There were no significant differences (P > 0.05) in the average substrate and ambient temperatures, and the volume of CO2 produced. This study revealed that it is more efficient to produce biogas using 40kg of rumen contents and 20 litres of tap water than producing biogas with 30kg of rumen contents and 30 litres of tap water or 20kg of rumen contents and 40 litres of tap water.Keywords: biogas, pH, rumen, temperature, wate

Improved forage cultivation for increased in fodder availability and climate change mitigation in the Savanna agro-ecological zone of northern Ghana

The study was conducted to investigate the biomass yield and quality of two forage species Brachiaria ruziziensis (B. ruziziensis) and Sorghum almum (S. almum) in a Randomized Complete Block Design (RCBD) in two different locations (Bihinayili in the Savelugu District and Zanlerigu in the Nabdam District) in the Savanna agro-ecological zone of Ghana. Agronomic data were collected and representative samples of forage biomass taken at 60 days after planting to estimate dry matter yield and nutritive quality. The two-way interaction effect of forage species and experimental site was not significant for both agronomic and chemical parameters except for tiller number. The average leaf size was broader (P=0.006) in S. almum (299.0 cm2) than B. ruziziensis (85.0 cm2). Number of leaves per plant was higher (P=0.016) in B. ruziziensis (10.75). Number of tillers per plant in B. ruziziensis (9.62) was higher (P=0.001) than S. almum (2.88). Plant height was however, higher in S. almum (183.1 cm) than B. ruziziensis (90.1 cm). Dry matter yield of forages at 60 days after planting was higher (P<0.001) at Bihinayili (8.49 tons/ha) than that at Zanlerigu (2.23 tons/ha). The CP content of the forages at Bihinayili (89.7 g/kg DM) was also higher (P=0.018) than that at Zanlerigu (68.6 g/kg DM). Dry matter yield of B. ruziziensis (4.84 tons/ha) did not differ significantly from that of S. almum (5.88 tons/ha). In conclusion, B. ruziziensis and S. almum performed well within the Savanna agroecological zone and could enhance fodder supply and carbon sequestration

Agronomic performance of five rice varieties and nutritive value of the straw from these varieties

Two separate experiments were conducted to assess the grain and straw yield (Exp. 1), chemical composition and in vitro gas production (Exp. 2) of five varieties of rice; Hybrid, Exbaika, Jasmine 85, IR841 and Long grain ordinary 2. Experiment 1 was conducted in a randomized complete block design with four replicates per variety. After harvesting, the rice straw from each variety was combined with Kapok leaf meal (KLM) at three inclusion levels (0, 25, 50%) to formulate a diet. The sole rice straw and formulated diets were analyzed for crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF) and Ash. Approximately 0.2 g of each diet (sole and formulated) was incubated in a McDougall’s buffered rumen fluid under anaerobic condition for the in vitro gas production. The varieties differed (P < 0.05) in relation to plant height, maturity days, percentage emergence, tiller number, straw yield and harvest index but did not differ in grain yield. The percentage emergence was in the range of 72.5 and 85.0%. with the highest (P = 0.003) recorded in the Hybrid variety. Plant height ranged from 90.5 to 110.8 cm with the highest (P = 0.046) reported in variety Long grain ordinary 2. Variety Long grain ordinary 2 had the longest (P<0.001) mean maturity days with the least recorded in the Hybrid variety. The highest (P < 0.05) straw yield was reported in variety Exbaika whilst Jasmine 85 had the least straw yield and harvest index. The CP concentration of the rice straw varieties increased numerically with an increase in the level of KLM. The NDF ranged from 622 g/kg DM to 913 g/kg DM for IR842 variety with 0% KLM and Long grain ordinary 2 variety with 25% KLM respectively. The ADF was in the range of 299.7 g/kg DM to 483.6 g/kg DM with the lowest reported in IR842 variety with 50% KLM. Mean asymptote gas production (b), fractional rate of gas production (c), in vitro gas production (IVGP) and in vitro organic matter digestibility (IVOMD) were not affected (P <0.05) by the variety x KLM inclusion level interaction. However, IVGP at 24 h and IVOMD both differed (P < 0.05) by variety. Varieties Jasmine 85, IR842 and Long grain ordinary 2 had higher IVGP and IVOMD as compared to the other two varieties. It was observed from the study that varieties IR841 and Long grain ordinary 2 provided higher grain and fodder production. The use of KLM as a replacement enhanced the nutrient composition, fermentation characteristics and digestibility

Effects of tropical browse plants on in vitro rumen protein degradability

Leaves of Multi-purpose trees (MPT) provide a very important ecosystem service in the form of feed for ruminant livestock in most tropical countries. They are often fed to ruminants as supplements due to their appreciable levels of crude protein and less concentration of recalcitrant cell wall fiber.The presence of condensed tannins in tropical MPT could minimise protein degradation in the rumen, reduce rumen ammonia concentration and ultimately reduce overall dry matter digestibility. In this study, eight MPT (Ceiba pentandra, Khaya senegalensis, Senna siamea, Ficus gnaphalocarpa, Pterocarpus erinaceus, Albizia lebbeck, Azadirachta indica and Gmelina arborea) were incubated with two levels of nitrogen to test the effect of condensed tannin on in vitro ammonia nitrogen concentration. The MPT’s in another experiment were incubated in sacco to determine the extent of protein degradability. The crude protein (CP) of the MPT was in the range of 92.2 to 229 g/kg DM. The neutral detergent fiber (NDF), acid detergent fiber (ADF) and acid detergent lignin (ADL) were in the range of 216-307 g/kg DM, 163.4 to 291 g/kg DM and 94.8-282 g/kg DM respectively. The highest condensed tannin (CT) was obtained in F gnaphalocarpa with no CT detected in A Lebbeck. There was no effect of the MPT and nitrogen buffer interaction on the in vitro gas production parameters at 72 h. There was no difference in in vitro dry matter digestibility (IVDMD) between the two nitrogen (N) buffered rumen fluids. Leaves of MPT incubated in nitrogen sufficient (NSf) buffer consistently had a higher NH3N concentration than the nitrogen deficient (ND). However, the NH3N concentration from the ND buffer remained within levels that ensured IVDMD was not different from that of NSf. The in sacco CP degradation characteristics differed among the leaves at 72 h. A. indica had a higher proportion of its protein content being soluble. The MPT with CT had lower proportion of their protein being degraded particularly in C. pentandra. Ammonia nitrogen concentration was maintained within limits that did not negatively affect IVDMD when the leaves of the MPT were incubated in ND and Nsf

In-Vitro Assessment of Antioxidant and Antimicrobial Activities of Methanol Extracts of Six Wound Healing Medicinal Plants

In this study, quantitative values of antioxidant activity of crude methanolic extracts of five Wound healing medicinal plants (Amaranthus spinosus, Anogeissus leiocarpus, Spondia monbin, Corchorus olitorius, and Mallotus oppositifolia) were investigated. The investigation used DPPH (1, 1-diphenyl-2-picrylhydrazyl) free radical as a substrate and Ferric reducing antioxidant power (FRAP) assay to determine both scavenging ability and the reducing properties. Antioxidant was further analysed quantitatively for flavonoid content, total phenolic content in the crude methanolic extracts using spectrophotometric assay. The result showed that all plants exhibited scavenging ability and strong reducing activity although the ability differed markedly among the various plant samples. The highest scavenging ability (% inhibition) was exhibited by A. leiocarpus (95.86 ± 0.1) followed by C. olitorius (94.19 ± 0.06) while the lowest was from A. spinosus (40.87±2.5). The reducing power was also highest in A. leiocarpus followed by S. monbin; while A. spinosus showed the least reducing power. In quantitative analysis, again A. leiocarpus was found to have the highest phenolic content (1294.81± 3.0 mg/g) with A. spinosus recording the least phenol and flavonoid content. The crude methanol extracts were also screened for their antimicrobial activity against four common pathogenic microorganisms (Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae and Citrobacter sp.) associated with wound infection by well diffusion method. All the extracts were found to inhibit the growth of both gram (+) and gram (-) bacteria organisms tested. Keywords: Radical scavenging effect, phenolic compounds, antioxidant activit

Primary beam effects of radio astronomy antennas -- II. Modelling the MeerKAT L-band beam

After a decade of design and construction, South Africa's SKA-MID precursor MeerKAT has begun its science operations. To make full use of the widefield capability of the array, it is imperative that we have an accurate model of the primary beam of its antennas. We have taken available L-band full-polarization 'astro-holographic' observations of three antennas and a generic electromagnetic simulation and created sparse representations of the beams using principal components and Zernike polynomials. The spectral behaviour of the spatial coefficients has been modelled using discrete cosine transform. We have provided the Zernike-based model over a diameter of 10 deg averaged over the beams of three antennas in an associated software tool (EIDOS) that can be useful in direction-dependent calibration and imaging. The model is more accurate for the diagonal elements of the beam Jones matrix and at lower frequencies. As we get more accurate beam measurements and simulations in the future, especially for the cross-polarization patterns, our pipeline can be used to create more accurate sparse representations of MeerKAT beams.Comment: 16 pages, 18 figures. This is a pre-copyedited, author-produced PDF of an article accepted for publication in MNRAS following peer review. The version of record [K. M. B. Asad et al., 2021] is available online at: https://doi.org/10.1093/mnras/stab10