Separation of valuable endogenous components from carrot peel by microwave extraction

With the rapid development of food technology, the amount of by-products generated is proportionally increasing as well. If not reused, by-products can pose a serious problem to our environment. In addition to managing and reusing such by-products, they may be valorised by extraction of valuable endogenous components using a less sophisticated, plain but modern technology such as microwave-assisted extraction. This new technique has been gaining popularity in recent years because compared to conventional methods, microwave-assisted extraction is characterised by shorter extraction times and lower solvent requirements. This preliminary experiment aimed to demonstrate that with the right operating parameters and solvent ratio, this method can be used for the efficient extraction of valuable endogenous components from carrot peels. To quantify the endogenous components, polyphenol content (TPC), antioxidant capacity (FRAP) and carotenoid content were investigated. The findings of this study showed that the optimal valuable substance extraction was obtained at 1:10 peelsolvent ratio at 100 W microwave power for fresh carrot peels, and 1:20 peel-solvent ratio at 800 W microwave power for dried carrot peels

Design, synthesis and biological evaluation of thiosemicarbazones, hydrazinobenzothiazoles and arylhydrazones as anticancer agents with a potential to overcome multidrug resistance

There is a constant need for new therapies against multidrug resistant (MDR) cancer. An attractive strategy is to develop chelators that display significant antitumor activity in multidrug resistant cancer cell lines overexpressing the drug efflux pump P-glycoprotein. In this study we used a panel of sensitive and MDR cancer cell lines to evaluate the toxicity of picolinylidene and salicylidene thiosemicarbazone, arylhydrazone, as well as picolinylidene and salicylidene hydrazino-benzothiazole derivatives. Our results confirm the collateral sensitivity of MDR cells to isatin-β-thiosemicarbazones, and identify several chelator scaffolds with a potential to overcome multidrug resistance. Analysis of structure-activity-relationships within the investigated compound library indicates that NNS and NNN donor chelators show superior toxicity as compared to ONS derivatives regardless of the resistance status of the cells. © 2016 Elsevier Masson SAS

Urológiai elváltozások nőgyógyászati tumoros betegeknél

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Prealbumin epitópok haptén hordozó szerepének vizsgálata limfocita transzformációs tesztben a gyógyszerallergia igazolására = The role of prealbumin as a hapten-carrier protein in lymphocyte transformation test for the establishment of drug allergy

Korábbi vizsgálataink alapján célul tűztük ki, hogy megvizsgáljuk a prealbumin egyes szerkezeti elemeinek haptén hordozó szerepét és különböző gyógyszerekhez kapcsolva nagyszámú betegben meghatározzuk, növelhető-e a limfocita transzformációs teszt (LTT) diagnosztikus hatékonysága. 119 biztosan gyógyszer érzékenységben szenvedő beteg csak gyógyszerrel ill. gyógyszer-prealbumin peptid konjugátummal végzett párhuzamos LTT vizsgálata során nem találtunk konzekvens különbséget a gyógyszer-peptid javára az LTT vizsgálat érzékenységében. Gyógyszeraktiválást követően a különböző immunglobulinok (IgD, IgM, IgG) sejtfelszíni kifejeződése individuálisan változott a betegek B limfocitáin. 84 gén expresszióját vizsgáltuk 2 egészséges egyén és 4 gyógyszerallergiában szenvedő beteg izolált limfocitáin a gyógyszerindukciót követően, amely gének a CD4+ helper T limfociták TH1-TH2 típusaival voltak kapcsolatban. A betegek esetében az érzékenyítő gyógyszer hatására sokkal több vizsgált gén kifejeződésének növekedése következett be. Az is megállapítható volt, hogy a TH1 és TH2 típusú válaszok egyaránt indukálódnak a gyógyszerallergiás betegeknél, bár egyénenként különböző kifejeződési mintázatot mutattak. Eredményeink megerősítik azt a vélekedést, hogy a gyógyszerallergia mind klinikai megjelenésében és lefolyásában, mind immunológiai mechanizmusaiban heterogén kórkép, a TH1 és TH2 típusú válaszok keverednek. | Human prealbumin acting as a hapten-carrier protein in drug allergy was suggested by our previous investigations. The aim of the work was that different structural elements of prealbumin acting as hapten-carrier peptides, in conjugation of different drugs, are able to increase the diagnostic sensitivity of lymphocyte transformation test (LTT) in drug allergy patients comparing to simple drugs. Gene expressions of drug-specific lymphocytes during drug activation were also investigated. Unfortunately, drug-prealbumin conjugates as activators were not able to increase the diagnostic sensitivity of LTT test comparing to simple drug activation based on detailed parallel measurements in 119 patients with proved drug allergy. Immunoglobulin expressions (IgD, IgM, IgG) on the drug-induced B lymphocytes of the patients are individually varied compared to freshly isolated B lymphocytes of the patients as well as to healthy controls.We investigated the expressions of 84 genes (in connection with TH1 and/or TH2 immune response) in freshly separated and drug-induced lymphocytes isolated from healthy controls and patients with drug allergy. The expressions of a lot of genes are increased in the drug-induced lymphocytes of drug allergy patients, however, their expression patterns are varied individually. Our results supported that drug allergy is a heterogeneous disorder in its clinical picture and immunological mechanisms. Both TH1 and TH2 immune responses are involved in the pathogenesis