201 research outputs found
EFFETS DE LA PYRIDOXINE SUR LE NIVEAU PORTAL DES ACIDES AMINĂS APRĂS UN REPAS PROTĂIQUE
No full textInternational audienc
A porin activity of purified λ-receptor protein from Escherichia coli in reconstituted vesicle membranes
No full text
Studies of the interaction of the maltose-binding protein of Escherichia coli, a closed-groove binder, with 4,6-O-ethylidenemalto-oligosaccharides (dp 2â5) and its regioselective labelling with 3-azibutyl 1-thio-α-(6-3H)maltoside
No full textDiffusion of solutes through channels produced by phage lambda receptor protein of Escherichiacoli: Inhibition by higher oligosaccharides of maltose series
No full textVirus-assisted loading of polymer nanocontainer
No full textWe present a DNA-containing polymeric nanocontainer using the self-assembled superstructure of amphiphilic block copolymers in aqueous solutions. To demonstrate that DNA translocation is possible across a completely synthetic block copolymer membrane, we have used a phage transfection strategy as a DNA-transfer model system. For this purpose the bacterial channel forming protein LamB was reconstituted in ABA-triblock copolymer vesicles. The outer membrane protein LamB is a specific transporter for maltodextrins but also serves as a receptor for λ phage to trigger the ejection of λ phage DNA. We demonstrate that the functionality of the LamB protein is fully preserved despite the artificial surrounding. This leads to a type of polymeric vehicle for DNA that could be useful for gene therapy
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