Relationship between the Dietary Fibre Consumption and the Level of

Frequent consumption of dietary fibres can have favourable effect in the prevention and treatment of several diseases. Despite of these positive effects, Hungarian consumption of dietary fibre, as well as fruits and vegetables are below the recommended levels. Thus the aim of this study was to analyse the fibre consumption habits, the related level of knowledge, as well as the recognition of the potential information sources to ensure authentic information about dietary fibres. For this purpose a questionnaire survey was completed with the help of 303 Hungarian participants in 2014. Results showed that participants with higher level of consumption of fruits and vegetables had higher level of knowledge about dietary fibres. Even though respondents were aware of the positive health effects of dietary fibre, their consumption habits do not reflect that. Internet was proved as a utilized and preferred information source, so stressing of the authentic websites for the consumers about dietary fibres is an outstanding task. Results indicated that regarding the multiple beneficial effects of dietary fibres, for the supporting of mindful consumer decisions accurate and reliable information must be ensured for consumers, as well as authentic sources have to be designated

A veszélyeztetett Anacamptodon splachnoides (Froel. ex Brid.) Brid. új adata a Bükk-hegységből = A new record of the threatened Anacamptodon splachnoides (Froel. ex Brid.) Brid. from the Bükk Mts. (NE Hungary)

Abstract – A new locality of the moss species Anacamptodon splachnoides (Froel. ex Brid.) Brid. was discovered in a Turkey oak-dominated forest stand in the Bükk Mts. (near Varbó village, North Hungarian Montains). A small population of this threatened bryophyte was found in a wet root-hole of a living Quercus cerris L. tree. Keywords: Amblystegiaceae, Bükk Mts., dendrotelm, knot-hole moss, North Hungarian Montains, Quercus cerris | Összefoglalás – A szerzők a veszélyeztetett Anacamptodon splachnoides (Froel. ex Brid.) Brid. mohafaj új adatát közlik a Bükk-hegységből, egy Varbó község melletti cseres–kocsánytalan tölgyesből. E veszélyeztetett lombosmohafaj kis populációját egy élő Quercus cerris fa gyökfőjének üregében azonosították. Kulcsszavak: Amblystegiaceae, Bükk-hegység, dendrotelma, Északi-középhegység, ritka mohafaj, Quercus cerri

Az élelmi rostokkal kapcsolatos fogyasztói ismeret – Hazai kérdőíves felmérés eredményei

Absztrakt Bevezetés: Az élelmi rostok az egészséges és kiegyensúlyozott táplálkozás kulcselemei, amelyek rendszeres fogyasztása számos betegség kockázatát csökkentheti. Célkitűzés: Jelen munka célja a magyar fogyasztók élelmi rostokkal kapcsolatos ismeretének, vásárlási döntésben betöltött szerepének feltárása, valamint a jelenleg használt és a fogyasztást előremozdító lehetséges információforrások megismerése volt. Módszer: A kérdőíves megkérdezést 303 magyar felnőtt fogyasztó segítségével végezték. Eredmények: Az élelmi rostok forrásait tekintve a résztvevők bizonytalanok voltak, míg a rostfogyasztás egészségügyi hatásainak ismerete viszonylag alapos volt. Az internet jelentős információs forrásként jelent meg a témában, azonban az eredmények rámutatnak az iskolai oktatás fontosságára is. Az élelmi rostban gazdag élelmiszereket gyakrabban fogyasztók, a nők, valamint a 45 éven felüli résztvevők az élelmi rostokkal kapcsolatosan tudatos attitűdöt mutattak. Következtetések: A hazai fogyasztók kiegyensúlyozott táplálkozásának érdekében az élelmi rostokkal kapcsolatos hiteles és gyakorlati elemeket tartalmazó iránymutatás és oktatás elengedhetetlen, amelynek eredményeképpen a latens ismeret várhatóan a fogyasztók vásárlási döntéseiben tudatosan is meg fog jelenni. Orv. Hetil., 2016, 157(8), 302–309

OWLAP - using OLAP approach in anomaly detection

OWLAP (Operative Workbench for Large-scale Analytics and Presentation) is a visual analytics tool that allows the user to browse and drill down the multidimensional data on-line with the possibility to export result into a zooming presentation framework. We address the challenges of multidimensional visualization by aiding the cognitively hard task of understanding attributes, finding patterns and outliers. We successfully solved the challenge of real time Big Data OLAP reporting by a home developed multithreaded inmemory database manager. Our additional focus is the automatic management of summary preparation that we aid by scripting the presentation framework of Prezi Inc

Razvoj i usporedba alternativnih metoda pročišćavanja adalimumaba izravno iz suspenzije stanične kulture

Research background. Protein A affinity chromatography is a well-established method currently used in the pharmaceutical industry. However, the high costs usually associated with chromatographic separation of protein A and the difficulties in continuous operation make the investigation of alternative purification methods very important. Experimental approach. In this study, extraction/back-extraction and precipitation/dissolution methods were developed and optimised. They were compared with protein A and cation exchange chromatography separations in terms of yield of monoclonal antibody (mAb) and amount of residual impurities, such as DNA and host cell proteins, and amount of mAb aggregates. For a comprehensive comparison of the different methods, experiments were carried out with the same cell-free fermentation broth containing adalimumab. Results and conclusions. Protein A and cation exchange chromatographic separations resulted in high yield and purity of adalimumab. The precipitation-based process resulted in high yield but with lower purity. The extraction-based purification resulted in low yield and purity. Thus, the precipitation-based method proved to be more promising than the extraction-based method for direct purification of adalimumab from harvested cell culture fluid. Novelty and scientific contribution. Although alternative purification methods may offer the advantages of simplicity and low-cost operation, further significant improvements are required to compete with the performance of chromatographic separations of adalimumab from true fermentation broth.Pozadina istraživanja. Afinitetna kromatografija pri kojoj se monoklonsko protutijelo veže za protein A je već dobro poznata metoda u farmaceutskoj industriji. Međutim, zbog visokih troškova izdvajanja proteina A pomoću kromatografije te poteškoća u provođenju kontinuiranog procesa važno je ispitati alternativne metode pročišćavanja. Eksperimentalni pristup. U ovome su radu razvijene i optimirane metode ekstrakcije sa ponovnom ekstrakcijom te taloženja i otapanja. Rezultati tih dvaju metoda, odnosno prinos monoklonskih protutijela, količina preostalih nečistoća, kao što su DNA i proteini stanica domaćina, te količina agregata monoklonskih protutijela, uspoređeni su s onima dobivenim afinitetnom kromatografijom na ionskom izmjenjivaču. Za sveobuhvatnu usporedbu različitih metoda, ispitivanja su provedena na identičnim hranjivim podlogama koje su sadržavale adalimumab. Rezultati i zaključci. Afinitetnom kromatografijom na ionskom izmjenjivaču dobiveni su veliki prinos i velika čistoća adalimumaba. Taloženjem su dobiveni veliki prinos, ali manja čistoća uzorka. Pročišćavanje pomoću ekstrakcije rezultiralo je manjim prinosom i manjom čistoćom uzorka. Zaključeno je da je metoda taloženja bolja od ekstrakcije za izravno pročišćavanje adalimumaba iz suspenzije stanične kulture. Novina i znanstveni doprinos. Iako alternativne metode imaju neke prednosti, kao što su jednostavna i jeftina primjena, neophodno ih je unaprijediti da bi dobiveni rezultati bili usporedivi s rezultatima dobivenim pročišćavanjem adalimumaba ionskom kromatografijom u hranjivoj podlozi

Increase in Alzheimer's related markers preceeds memory disturbances: Studies in vasopressin-deficient Brattleboro rat

Alzheimer's disease (AD) is the most common form of dementia in the elderly. For more effective therapy early diagnostic markers could be beneficial. Therefore we compared one year old rats with adults and examined if changes in possible brain markers of AD preceeded memory decline. We also tested if vasopressin-deficient animals were useful model of AD as vasopressin has well known positive effect on memory and AD patient has decreased vasopressin production. We compared adult (3 month) and old (12 month), normal and vasopressin-deficient Brattleboro rats. To receive a comprehensive picture about their memory we examined their social discrimination, object discrimination and conditioned learning abilities (shuttle box). Amyloid precursor protein (APP), mitogen-activated protein kinase 1 (MAPK1), β-actin and tryptophan 2,3-dioxygenase 2 (TDO2) mRNA levels was measured by quantitative PCR. There was no difference between the memory of adult and aged groups. The vasopressin-deficient rats at both ages showed a weaker performance in the course of social and object discrimination tests and a higher escape failure during the shuttle box experiment. The brain marker mRNAs of the elder animals were higher than the levels of the adults, but the absence of vasopressin had no influence on them. Thus, the one year old rats showed elevated levels of AD-related markers, but memory deficits were observable only in vasopressin deficient animals. Vasopressin does not seem to have pathogenic role in AD. Changes in the studied markers might predict later symptoms, although further studies are required for confirmation

Consumption of fibre rich foods: comparative study in different countries

Foods such as fruit, vegetables, and cereals, and particularly whole grain, are rich in dietary fibre and have been proved to have multiple beneficial effects for the human health. The present research was designed to assess some eating practices related to fibre-rich foods in different countries, namely Argentina, Croatia, Hungary, Latvia, Portugal, and Romania. A cross-sectional descriptive study was undertaken on a sample of 4905 adult participants, obeying all ethical guidelines for this type of research. Regarding the data treatment, basic statistics was complemented with the tree classification analysis. Generally, the results show a low consumption of salads and vegetables, i.e., up to 11 servings/week (for 78.2% of participants), with Croatia in the lead (86.6%). Regarding fruit, a great majority of data also indicated low consumption (92.3%), most especially for Latvia (98.3%). The level of consumption of whole cereals was also low (72.6%), particularly for Latvia (90.0%). The tree classification analysis showed that while the first discriminant variable for the consumption of salads and vegetables was country, followed by education, for the consumption of fruit, it was country and then sex, and finally, for the consumption of whole cereals, it was sex and followed by country. The results allowed the conclusion that the consumption of foods rich in dietary fibre was very low for these countries, highlighting the necessity to implement strategies that incentivise the consumption of such foods, which are very important for a healthy diet