The effect of alkalisation on the mechanical properties of natural fibres

A study on the effect of alkalisaton using 3% NaOH solution was carried out on Flax, Kenaf, Abaca and Sisal to observe the impact that the common pre-treatment process has on fibre mechanical properties. The result of the investigation indicated that over-treatment of natural fibres using NaOH could have a negative effect on the base fibre properties. It is concluded that a treatment time of less than 10 minutes is sufficient to remove hemicelluloses and to give the optimum effect

Vacuum infusion of natural fibre composites for structural applications

Numerous methods of manufacturing natural fibre composites have been reported in the literature, including compression moudling, often in conjunction with a hot press. Other forms of composite manufacture include 'Vacuum Assisted Resin Transfer Moulding' (VATRM) and the 'Seemann Composite Resin Infusion Moulding Process' (SCRIMP). These methods have been reported to produce natural fibre composies with reasonable mechanical properties [1-2]. In this paper, a vacuum infusion rig is described that has been developed to produce consistent quality composite plates for studies into optimising natural fibre composites. The process aims to harness the benefits of vacuum infusion and compression moulding, where vacuum infusion encourages the removal of trapped air in the system and hence avoid reduction, and additional compression moulding can help to achieve high volume fractions that are otherwise difficult in other processes

Mechanical testing of natural fibre reinforced polyester resin composites and Mode 1 fracture toughness testing of resin blocks

Recent European Parliament directive requires companies to achieve materials recycling greater than 80% in particular in the automotive sector [1]. The research on natural fibre based composite materials fits well into this ecological image. The advantages of natural fibres over synthetic materials include, low density, relative cheapness, availability and biodegradability. In this paper we explore the fabrication and mechanical testing of natural fibre composites and this is part of an on going study at Strathclyde University and describes the fabrication of composites using natural fibre and styrene polyester resin. The properties of the synthetic resin can be varied by changing the catalysts concentration and flexural (three point bending) and single-edged notched bending (SENB) properties are reported at different concentrations of the catalyst

Tensile testing of cellulose based natural fibers for structural composite applications

A series of tensile tests were conducted on a Lloyd LRX tensile testing machine for numerous natural fibers deemed potential candidates for development in composite applications. The tensile tests were conducted on the fibers jute, kenaf, flax, abaca, sisal, hemp, and coir for samples exposed to moisture conditions of (1) room temperature and humidity, (2) 65% moisture content, (3) 90% moisture content, and (4) soaked fiber. These seven fibers were then tested for the four conditions and the mechanical properties of tensile strength, tensile strain to failure, and Young's modulus were calculated for the results. These results were then compared and verified with those from the literature, with some of the fibers showing distinctly promising potential. Additionally, a study on the effect of alkalization using 3% NaOH solution was carried out on flax, kenaf, abaca, and sisal to observe impact that this common fiber pre-treatment process has on fiber mechanical properties. The result of the investigation indicated that over treatment of natural fibers using NaOH could have a negative effect on the base fiber properties. It is consequently apparent that a treatment time of less than 10 min is sufficient to remove hemicelluloses and to give the optimum effect

PCNA ubiquitylation ensures timely completion of unperturbed DNA replication in fission yeast

PCNA ubiquitylation on lysine 164 is required for DNA damage tolerance. In many organisms PCNA is also ubiquitylated in unchallenged S phase but the significance of this has not been established. Using Schizosaccharomyces pombe, we demonstrate that lysine 164 ubiquitylation of PCNA contributes to efficient DNA replication in the absence of DNA damage. Loss of PCNA ubiquitylation manifests most strongly at late replicating regions and increases the frequency of replication gaps. We show that PCNA ubiquitylation increases the proportion of chromatin associated PCNA and the co-immunoprecipitation of Polymerase δ with PCNA during unperturbed replication and propose that ubiquitylation acts to prolong the chromatin association of these replication proteins to allow the efficient completion of Okazaki fragment synthesis by mediating gap filling

Cellulose based composite materials

Natural fibre composites are a fast growing research area, with many observable research branches. In this thesis, studies into natural fibre composites are undertaken. This includes work into the base fibre mechanical properties, pre-processing techniques and the influence of alkalisation and silanation, both common fibre processing methods used to improve interfacial properties. The effects of these pre-processing techniques were also evaluated using Fourier transform infrared spectroscopy (FT-IR). It was observed that the processing had shown definite signs of altering the surface functional groups. For the studies into the base fibre strengths, it was found that natural fibres are highly variable. with the testing complicated by difficulties in measuring cross sectional areas. It was also found that natural fibres are sensitive to moisture, which affects their mechanical properties somewhat, although no conclusive trends were derived.EThOS - Electronic Theses Online ServiceGBUnited Kingdo

Genome-scale CRISPR screening reveals that C3aR signaling is critical for rapid capture of fungi by macrophages.

The fungal pathogen Histoplasma capsulatum (Hc) invades, replicates within, and destroys macrophages. To interrogate the molecular mechanisms underlying this interaction, we conducted a host-directed CRISPR-Cas9 screen and identified 361 genes that modify macrophage susceptibility to Hc infection, greatly expanding our understanding of host gene networks targeted by Hc. We identified pathways that have not been previously implicated in Hc interaction with macrophages, including the ragulator complex (involved in nutrient stress sensing), glycosylation enzymes, protein degradation machinery, mitochondrial respiration genes, solute transporters, and the ER membrane complex (EMC). The highest scoring protective hits included the complement C3a receptor (C3aR), a G-protein coupled receptor (GPCR) that recognizes the complement fragment C3a. Although it is known that complement components react with the fungal surface, leading to opsonization and release of small peptide fragments such as C3a, a role for C3aR in macrophage interactions with fungi has not been elucidated. We demonstrated that whereas C3aR is dispensable for macrophage phagocytosis of bacteria and latex beads, it is critical for optimal macrophage capture of pathogenic fungi, including Hc, the ubiquitous fungal pathogen Candida albicans, and the causative agent of Valley Fever Coccidioides posadasii. We showed that C3aR localizes to the early phagosome during Hc infection where it coordinates the formation of actin-rich membrane protrusions that promote Hc capture. We also showed that the EMC promotes surface expression of C3aR, likely explaining its identification in our screen. Taken together, our results provide new insight into host processes that affect Hc-macrophage interactions and uncover a novel and specific role for C3aR in macrophage recognition of fungi