Lipid Profiles and Its Association with Pre-Eclampsia and Eclampsia in Nulliparous Pregnant Women

Objectives: To access the lipid profiles and its association with pre-eclampsia and eclampsia in nulliparous pregnant women. Material and Methods: This cross-sectional study was performed on 234 nulliparous pregnant women with gestation period of  > 20 weeks and aged 15-45 years, from March to October 2014, in Institute of Basic Medical Sciences (IBMS), Khyber Medical University (KMU), Peshawar, Pakistan with cooperation from gynecology and obstetrics departments of three tertiary-care hospitals of Peshawar & Khyber Medical College, Peshawar,  Khyber Pakhtunkhwa (KP), Pakistan. Subjects were assigned to three groups i.e., group A, group B and group C. For performing biochemical assays and lipid profiling, through ELISA, blood samples were collected from already subjects both with the disease and the controls. Results: Both subjects having pre-eclampsia and eclampsia showed significant elevated levels (p < 0.001) for low density lipoproteins cholesterols (LDL-c), high density lipoprotein cholesterol (HDL-c), total cholesterol (TC)/HDL-c and LDL-c/HDL-c ratio. But TC of pre-eclampsia subjects were found significantly elevated (p < 0.001) in relation to controls. LDL-c/HDL-c and TC/HDL-c also revealed an elevated significant change (p < 0.001) both for pre-eclamptic and eclamptic subjects. On the other hand, only TG/HDL-c in pre-eclamptic patients was found significantly higher (p<0.004) when compared to control group.  Conclusion: Serum lipid levels were observed higher in pre-eclampsia and eclampsia patients hence an early assessment is necessary to prevent complications in such patients

Genetic dissection of Ni toxicity in a spring wheat diversity panel by using 90 K SNP array

Excess Ni intake has harmful implications on human health, which include chronic bronchitis, reduced lung function, and cancer of lung and nasal sinuses. Like other toxic metals, higher Ni accumulation in grains leads to excess intake by humans when the contaminated grains are consumed as food. There is little information about the genetic factors that regulate Ni uptake in plants. To investigate genetic architecture of Ni uptake in leaf and translocation to grain, we performed a genome-wide association study with genotyping from 90 K array in a historical bread wheat diversity panel from Pakistan. We observed that Ni toxicity caused more than 50 % reductions in biological yield and grain yield, other agronomic traits were also partly or severely affected. Genetic association study helped identify 23 SNP-trait associations involved in Ni uptake in leaf and translocation to grains. These 23 SNPs covered 15 genomic loci at chromosomes 1A, 2D, 3B, 4A and 4B of wheat. The favorable alleles of these SNPs were randomly distributed in subpopulations indicating no selection pressure for this trait during breeding improvement. These regions had 283 low-confidence and 248 high-confidence protein coding genes. Among these, 156 were annotated using databases of wheat and closely related grass species. Since there is no previous report on genetic information of Ni uptake and translocation, these results provide sufficient grounds for further research of candidate genes and varietal development.Peer reviewe

Burnout among surgeons before and during the SARS-CoV-2 pandemic: an international survey

Background: SARS-CoV-2 pandemic has had many significant impacts within the surgical realm, and surgeons have been obligated to reconsider almost every aspect of daily clinical practice. Methods: This is a cross-sectional study reported in compliance with the CHERRIES guidelines and conducted through an online platform from June 14th to July 15th, 2020. The primary outcome was the burden of burnout during the pandemic indicated by the validated Shirom-Melamed Burnout Measure. Results: Nine hundred fifty-four surgeons completed the survey. The median length of practice was 10 years; 78.2% included were male with a median age of 37 years old, 39.5% were consultants, 68.9% were general surgeons, and 55.7% were affiliated with an academic institution. Overall, there was a significant increase in the mean burnout score during the pandemic; longer years of practice and older age were significantly associated with less burnout. There were significant reductions in the median number of outpatient visits, operated cases, on-call hours, emergency visits, and research work, so, 48.2% of respondents felt that the training resources were insufficient. The majority (81.3%) of respondents reported that their hospitals were included in the management of COVID-19, 66.5% felt their roles had been minimized; 41% were asked to assist in non-surgical medical practices, and 37.6% of respondents were included in COVID-19 management. Conclusions: There was a significant burnout among trainees. Almost all aspects of clinical and research activities were affected with a significant reduction in the volume of research, outpatient clinic visits, surgical procedures, on-call hours, and emergency cases hindering the training. Trial registration: The study was registered on clicaltrials.gov "NCT04433286" on 16/06/2020

Inhibitory Effects of Glycyrrhiza glabra

Glycyrrhiza glabra L. (Leguminosae) is widely used in folk medicines. Glycyrrhizin, an active compound of G. glabra, possesses anti-inflammatory activity. This study investigates the G. glabra methanol extract and glycyrrhizin for the treatment of corneal neovascularization (CNV). G. glabra was extracted in 70% aqueous methanol. Phytochemical tests, thin layer chromatography (TLC), and high performance liquid chromatography (HPLC) were used for the analysis of chemical composition. The topical solution of G. glabra methanol extract (2% w/v) and glycyrrhizin (1% w/v) was prepared in normal saline. After corneal burn (1 N NaOH), animals were left untreated for a week so that neovascularization appears in all groups. Treatments started on day 7 and continued for next 21 consecutive days. The animals were treated with 3 drops of various topical solutions thrice a day. Digital photograph analysis and histological studies were used for the evaluation of CNV. Phytochemical analysis of the G. glabra methanol extract showed the presence of saponins, phenols, carbohydrates, flavonoids, and proteins. TLC and HPLC confirmed the presence of glycyrrhizin. Photograph analysis of the extract and glycyrrhizin treated group showed a considerable decrease in CNV. Histological study of G. glabra and glycyrrhizin treated groups showed no blood vessels with properly arranged collagen fibers. This study showed that G. glabra and glycyrrhizin can be used for the treatment of CNV. Bioassay guided isolation can lead to preparation of ophthalmic solutions for the treatment of CNV

An Innovative Clustering Hierarchical Protocol for Data Collection from Remote Wireless Sensor Networks Based Internet of Things Applications

Recently, unmanned aerial vehicles (UAVs) have emerged as a viable solution for data collection from remote Internet of Things (IoT) applications. However, the successful implementation in this regard necessitates the development of a reliable and energy-efficient routing protocol. This paper proposes a reliable and an energy-efficient UAV-assisted clustering hierarchical (EEUCH) protocol designed for remote wireless sensor networks (WSNs) based IoT applications. The proposed EEUCH routing protocol facilitates UAVs to collect data from ground sensor nodes (SNs) that are equipped with wake-up radios (WuRs) and deployed remotely from the base station (BS) in the field of interest (FoI). During each round of the EEUCH protocol, the UAVs arrive at the predefined hovering positions at the FoI, perform clear channel assignment, and broadcast wake-up calls (WuCs) to the SNs. Upon receiving the WuCs by the SNs’ wake-up receivers, the SNs perform carrier sense multiple access/collision avoidance before sending joining requests to ensure reliability and cluster-memberships with the particular UAV whose WuC is received. The cluster-member SNs turn on their main radios (MRs) for data packet transmission. The UAV assigns time division multiple access (TDMA) slots to each of its cluster-member SNs whose joining request is received. Each SN must send the data packets in its assigned TDMA slot. When data packets are successfully received by the UAV, it sends acknowledgments to the SNs, after which the SNs turn off their MRs, completing a single round of the protocol. The proposed EEUCH routing protocol with WuR eliminates the issue of cluster overlapping, improves the overall performance, and increases network stability time by a factor of 8.7. It also improves energy efficiency by a factor of 12.55, resulting in a longer network lifespan compared to Low Energy Adaptive Clustering Hierarchy (LEACH) protocol. Moreover, EEUCH collects 5.05 times more data from the FoI than LEACH. These results are based on simulations in which the EEUCH protocol outperformed the existing six benchmark routing protocols proposed for homogeneous, two-tier, and three-tier heterogeneous WSNs

TAMEC: Trusted Augmented Mobile Execution on Cloud

Cloud computing has emerged as an attractive platform for individuals and businesses to augment their basic processing capabilities. Mobile devices with access to Internet are also turning towards clouds for resource-intensive tasks by working out a trade-off between resources required for performing computation on-device against those required for off-loading task to the cloud. However, as with desktop clients, mobile clients face significant concerns related to confidentiality and integrity of data and applications moved to and from the cloud. Cloud-related security solutions proposed for desktop clients could not be readily ported to mobile clients owing to the obvious limitation in their processing capabilities and restrained battery life. We address this problem by proposing architecture for secure exchange and trusted execution between mobile devices and cloud hosts. We establish a symmetric-key-based secure communication channel between mobile and cloud, backed by a trusted coordinator. We also employee a Trusted Platform Module- (TPM-) based attestation of the cloud nodes on which the data and applications of mobile device will be hosted. This gives a comprehensive solution for end-to-end secure and trusted interaction of the mobile device with cloud hosts

Dyeing of Wool with Sappan Wood Natural Dye Using Metal Salts for Enhancement in Color and Fastness Properties

In this study, we explore the dyeing properties of the extract of sappan wood natural dye for coloration of wool yarn to find the potential use of natural dyed wool yarn in the development of eco-friendly textiles. Different characterizations were used to know the chemistry of sappan wood dye and its interaction with metal salts and wool. Interestingly, we found that metal salts, which are called as mordants in reference to unmordanted dyed sample, play an important role in fixing the dye molecule on wool as well as in creating different shades with a variety of hues and tones. The typical color of fabric dyed with extracts of sappan wood without mordant varies from yellowish to red. Light yellowish shades were obtained in case of aluminum sulfate-mordanted samples, whereas yellowish shades were obtained in case of ferrous sulfate mordant. Light colorfastness of the premordanted dyed samples was relatively better than that of the unmordanted dyed samples. The wash fastness of all samples mordanted as well as unmordanted shows color change fairly good to good level, whereas the color staining on wool and cotton was found to be negligible