DEVELOPMENT OF A NOVEL VEHICLE GUIDANCE SYSTEM: VEHICLE RISK MITIGATION AND CONTROL

Over a half of fatal vehicular crashes occur due to vehicles leaving their designated travel lane and entering other lanes or leaving the roadway. Lane departure accidents also result in billions of dollars in cost to society. Recent vehicle technology research into driver assistance and vehicle autonomy has developed to assume various driving tasks. However, these systems are do not work for all roads and travel conditions. The purpose of this research study was to begin the development a novel vehicle guidance approach, specifically studying how the vehicle interacts with the system to detect departures and control the vehicle A literature review was conducted, covering topics such as vehicle sensors, control methods, environment recognition, driver assistance methods, vehicle autonomy methods, communication, positioning, and regulations. Researchers identified environment independence, recognition accuracy, computational load, and industry collaboration as areas of need in intelligent transportation. A novel method of vehicle guidance was conceptualized known as the MwRSF Smart Barrier. The vision of this method is to send verified road path data, based AASHTO design and vehicle dynamic aspects, to guide the vehicle. To further development research was done to determine various aspects of vehicle dynamics and trajectory trends can be used to predict departures and control the vehicle. Tire-to-road friction capacity and roll stability were identified as traits that can be prevented with future road path knowledge. Road departure characteristics were mathematically developed. It was shown that lateral departure, orientation error, and curvature error are parametrically linked, and discussion was given for these metrics as the basis for of departure prediction. A three parallel PID controller for modulating vehicle steering inputs to a virtual vehicle to remain on the path was developed. The controller was informed by a matrix of XY road coordinates, road curvature and future road curvature and was able to keep the simulated vehicle to within 1 in of the centerline target path. Recommendations were made for the creation of warning modules, threshold levels, improvements to be applied to vehicle controller, and ultimately full-scale testing. Advisor: Cody S. Stoll

Patterson Irrigation District

Presented at the 2002 USCID/EWRI conference, Energy, climate, environment and water - issues and opportunities for irrigation and drainage on July 9-12 in San Luis Obispo, California.Includes bibliographical references.Making accurate, informed operational decisions in water and energy management can have significant resource and fiscal impacts on irrigation districts. The need for accurate and reliable real-time and historical data is key in making these vital decisions. The use of every acre-foot of water and every kilowatt-hour of energy, resource management, has become the topic of scrutiny in today's world. The protection of these valuable water and energy resources, held in trust and managed by the irrigation district, on behalf of its' landowner constituents, is one of the vital functions of the Patterson Irrigation District (PIO). Plant Control and Supervisory Control and Data Acquisition (SCADA) systems can provide the link between data and effective District operations and management. This case study will outline the initial development, expansion and subsequent upgrade of the Patterson Irrigation District's Plant Control and SCADA systems, the role in data acquisition and daily district operations, the benefits the district and its water users have accrued from accurate real-time and historical data and finally, the lessons learned in the development, implementation and evolution of a state-of-the-art Plant Control and SCADA system for irrigation district use. In its first full year of operation, 1999, historical data verified an increase of 23% in total Station #1 pumping plant efficiency on a kW-hr per acre-foot basis

Improved Vectors for Selecting Resistance to Hygromycin

Resistance to hygromycin B is an important dominant selectable marker in fungal transformation. Our goal was to improve vectors for hygromycin selection by making the gene more compact, by eliminating sites for commonly used restriction enzymes, and by subcloning the modified gene into convenient vectors. These improvements were made by modifying pCSN43 (Staben et al. 1989 Fungal Genetics Newsl. 36:79-81) through three rounds of megaprimer mutagenesis (Aiyar and Leis, 1993 Biotechniques 14:366-368 ), a technique based on polymerase chain reaction amplification. Plasmid pCSN43 has a 2.4 kb SalI fragment containing the bacterial hph gene (Gritz and Davies, 1983 Gene 25:179-188), encoding hygromycin B phosphotransferase, under control of the Aspergillus nidulans trpC promoter and terminator (Mullaney et al. 1985 MGG 199:37-45

Pipeline and Canal Downstream Control System For Recirculation - Patterson ID Case Study

Patterson ID in Central California has five long, along-the-contour lateral canals that flow northward from a main canal. The main canal operates on downstream control, providing excellent flexibility to heads of the laterals. The lateral canals operate with manual upstream control and have little storage. The classic tail-ender problem existed; spill from the tail end was necessary to avoid under-supplying tail-end customers. To eliminate the spill and to provide better flexibility, a system was designed and constructed to tie the ends of the five laterals together with pipes and pumps, with one central regulating reservoir. The automatic control system allows water to exit a lateral pool by gravity if that particular lateral end has too high a water level. Conversely, if the most downstream pool on a lateral canal drops, a VFD-equipped pump from a downhill lateral automatically supplies the correct flow rate to re-establish a constant water level. The same pipe is used for flow in both directions. Any excess flow from the system as a whole is automatically routed to the reservoir. Any deficit from the system as a whole is removed from the reservoir. The complete system is monitored by SCADA, so operators know where excesses or deficits occur, and can adjust flows at the heads of the laterals to compensate for mismatches at the ends of the laterals. The system has worked successfully for three irrigation seasons. The paper describes the control philosophy, design, costs, challenges, and benefits

A series of vectors for fungal transformation

We report a new fungal selectable marker that confers resistance to chlorimuron ethyl, a sulfonylurea herbicide. This gene as well as genes that confer resistance to hygromycin and bialaphos have been engineered to be compact and to eliminate sites for most common restriction enzymes. These three selectable markers have been used to construct a series of vectors for fungal transformation

A miniprep procedure for isolating genomic DNA from Magnoporthe grisea

We have developed a simple miniprep procedure for the isolation of genomic DNA from the ascomycete Magnaporthe grisea. This pathogen of many grasses, including rice, has a moderate growth rate and produces intermediate to low numbers of conidia when grown in culture. Thus, in our previous DNA preparation procedure we inoculated swirling liquid cultures with mycelium that had been fragmented in a blender rather than with conidia. The mycelium obtained from these cultures was ground in liquid nitrogen for DNA extraction. Though the quantity and quality of DNA obtained by this method is satisfactory, the technique is too laborious for analysis of many strains. We developed the procedure described below to eliminate the need to fragment mycelium in a blender to inoculate cultures and to eliminate the need to grind mycelium in liquid nitrogen for DNA extraction. The new procedure, which relies on the enzymatic removal of cell walls and the lysis of protoplasts, should be readily adaptable to other filamentous fungi with growth characteristics similar to those of M. grisea

HYR1-Mediated Detoxification of Reactive Oxygen Species Is Required for Full Virulence in the Rice Blast Fungus

During plant-pathogen interactions, the plant may mount several types of defense responses to either block the pathogen completely or ameliorate the amount of disease. Such responses include release of reactive oxygen species (ROS) to attack the pathogen, as well as formation of cell wall appositions (CWAs) to physically block pathogen penetration. A successful pathogen will likely have its own ROS detoxification mechanisms to cope with this inhospitable environment. Here, we report one such candidate mechanism in the rice blast fungus, Magnaporthe oryzae, governed by a gene we refer to as MoHYR1. This gene (MGG_07460) encodes a glutathione peroxidase (GSHPx) domain, and its homologue in yeast was reported to specifically detoxify phospholipid peroxides. To characterize this gene in M. oryzae, we generated a deletion mutantΔhyr1 which showed growth inhibition with increased amounts of hydrogen peroxide (H2O2). Moreover, we observed that the fungal mutants had a decreased ability to tolerate ROS generated by a susceptible plant, including ROS found associated with CWAs. Ultimately, this resulted in significantly smaller lesion sizes on both barley and rice. In order to determine how this gene interacts with other (ROS) scavenging-related genes in M. oryzae, we compared expression levels of ten genes in mutant versus wild type with and without H2O2. Our results indicated that the HYR1 gene was important for allowing the fungus to tolerate H2O2 in vitro and in planta and that this ability was directly related to fungal virulence

“Taking the Pulse of Jefferson Primary Care: Provider and Team Wellness Survey Results and Next Steps”

Agenda Context (Randa Sifri) Results (Amy Cunningham) Action Steps (Keith Sweigard) Q&