Measurement of Flow Angularity at Supersonic and Hypersonic Speeds with the Use of a Conical Probe

The characteristics of a 40 deg half-angle cone for measuring flow angularity were determined experimentally. Tests were conducted at a Mach number of 21 in helium with check points at Mach number 3.55 in air for angles of pitch up to 5 deg. The air tests confirmed theoretical indications of small or negligible Mach number and test-medium effects for the case of air and helium. The instrument is capable of measuring flow angularity at high Reynolds numbers and speeds greater than that necessary for shock attachment to within (+/-)1/3 deg

Graduate Recital: Gibson Swalley, Violin; YounHee Choi, Piano; April 27, 2024

Kemp Recital HallApril 27, 2024Saturday Evening7:30 p.m

Self-Perceptions of Leadership Ability and Achieving Styles of Female Student-Athletes

This study examined female student-athletes' self-reported leadership ability and achieving styles and the relationship of individual and team sport female student-athletes' self-perceptions of leadership ability and achieving style preferences. An on-online survey consisting of a composite variable of 12 leadership-indicator items and the Achieving Styles Inventory was used to examine the research questions. The sample included 30 female student-athletes competing in Division I National Collegiate Athletic Association athletics at a Mid-Atlantic public institution. The results indicated individual sport female student-athletes have a significantly greater preference for using the Competitive Direct Achieving Style than team sport student-athletes. While individual and team sport female student-athletes demonstrated a similar perception of leadership ability, the team sport student-athletes consistently saw their achieving practices as being leaderly while the individual sport student-athletes saw only the Power Direct achieving style as being leaderly. Implications for enhancing student-athletes' relational leadership capacities are discussed

Startup Of A Large Compression Train - Testing Verifies Design.

LecturePg. 65-72The startup of a reliable compressor train involves not only the design and design audit, but also startup testing to verify the functionality of the design. Startup testing usually includes mechanical and electrical system checks, solo runs, alignment measurements, and extensive vibration monitoring. There are other test programs conducted at startup that can also play an important role in the successful startup of a critical process train. Recently; a large compressor train was installed and brought on stream in an air oxidation process. Described briefly herein are five tests that helped verify the design and that are contributing to the reliability of the train. These tests were: • shaker testing a low tuned concrete foundation, • transient torsional torque measurement during startup, • measurement of stator vane stresses during surge, • compressor surge tests, and • measuring rated point performance

Aliphatic/aromatic amino acid pairings for polyamide recognition in the minor groove of DNA

Selective placement of an aliphatic β-alanine (β) residue paired side-by-side with either a pyrrole (Py) or imidazole (Im) aromatic amino acid is found to compensate for sequence composition effects for recognition of the minor groove of DNA by hairpin pyrrole−imidazole polyamides. A series of polyamides were prepared which contain pyrrole and imidazole aromatic amino acids, as well as γ-aminobutyric acid (γ) “turn” and β-alanine “spring” aliphatic amino acid residues. The binding affinities and specificities of these polyamides are regulated by the placement of paired β/β, Py/β, and Im/β residues. Quantitative footprint titrations demonstrate that replacing two Py/Py pairings in a 12-ring hairpin (6-γ-6) with two Py/β pairings affords 10-fold enhanced affinity and similar sequence specificity for an 8-bp target sequence. The 6-γ-6 hairpin ImPyImPyPyPy-γ-ImPyPyPyPyPy-β-Dp, which contains six consecutive amino acid pairings, is unable to discriminate a single-base-pair mismatch site 5‘-TGTTAACA-3‘ from a 5‘-TGTGAACA-3‘ match site. The hairpin polyamide Im-β-ImPyPyPy-γ-ImPyPyPy-β-Py-β-Dp binds to the 8-bp match sequence 5‘-TGTGAACA-3‘ with an equilibrium association constant of Ka = 2.4 × 1010 M-1 and ≥48-fold specificity versus the 5‘-TGTTAACA-3‘ single-base-pair mismatch site. Modeling indicates that the β-alanine residue relaxes ligand curvature, providing for optimal hydrogen bond formation between the floor of the minor groove and both Im residues within the Im-β-Im polyamide subunit. This observation provided the basis for design of a hairpin polyamide, Im-β-ImPy-γ-Im-β-ImPy-β-Dp, which incorporates Im/β pairings to recognize a “problematic” 5‘-GCGC-3‘ sequence at subnanomolar concentrations. These results identify Im/β and β/Im pairings that respectively discriminate G·C and C·G from A·T/T·A as well as Py/β and β/Py pairings that discriminate A·T/T·A from G·C/C·G. These aliphatic/aromatic amino acid pairings will facilitate the design of hairpin polyamides which recognize both a larger binding site size as well as a more diverse sequence repertoire

Charles W. Bolen Faculty Recital Series: Faculty String Quartet: Dr. Igor Kalnin, Violin; Gibson Swalley, Violin; Katherine Lewis, Viola; Cora Swenson Lee, Cello; Kimberly Risinger, Flute; April 2, 2024

Kemp Recital HallApril 2, 2024Tuesday Evening7:30 p.m

Faculty Recital Series: Faculty String Quartet: Igor Kalnin, Violin; Gibson Swalley, Violin; Katherine Lewis, Viola; Cora Swenson Lee, Cello; Tuyen Tonnu, Piano; November 3, 2022

Kemp Recital HallNovember 3, 2022Thursday Evening7:30 p.m

Charles W. Bolen Faculty Recital Series: Illinois State University Faculty String Quartet: Igor Kalnin, Violin; Gibson Swalley, Violin; Katherine Lewis, Viola; Cora Swenson Lee, Cello; David Gresham, Clarinet; October 30, 2023

Kemp Recital HallOctober 30, 2023Monday Evening 7:30 p.m

Faculty String Quartet: Igor Kalnin, Violin; Gibson Swalley, Violin; Katherine Lewis, Viola; Cora Swenson Lee, Cello; Benjamin de Kock, Double Bass; April 4, 2023

Kemp Recital HallApril 4, 2023Tuesday Evening7:30 p.m

Enhancing the cellular uptake of Py–Im polyamides through next-generation aryl turns

Pyrrole–imidazole (Py–Im) hairpin polyamides are a class of programmable, sequence-specific DNA binding oligomers capable of disrupting protein–DNA interactions and modulating gene expression in living cells. Methods to control the cellular uptake and nuclear localization of these compounds are essential to their application as molecular probes or therapeutic agents. Here, we explore modifications of the hairpin γ-aminobutyric acid turn unit as a means to enhance cellular uptake and biological activity. Remarkably, introduction of a simple aryl group at the turn potentiates the biological effects of a polyamide targeting the sequence 5′-WGWWCW-3′ (W = A/T) by up to two orders of magnitude. Confocal microscopy and quantitative flow cytometry analysis suggest this enhanced potency is due to increased nuclear uptake. Finally, we explore the generality of this approach and find that aryl-turn modifications enhance the uptake of all polyamides tested, while having a variable effect on the upper limit of polyamide nuclear accumulation. Overall this provides a step forward for controlling the intracellular concentration of Py–Im polyamides that will prove valuable for future applications in which biological potency is essential