Behavioral analysis of the effects of long-term treatment with L-ornithine in mice

L-ornithine has recently received considerable attention as an anxiolytic-like or antifatigue agent in both human and animal studies. However, most previous results were obtained from a single administration of L-ornithine. Accordingly, this study was designed to clarify the effects of long-term L-ornithine treatment on psychological stress and locomotor activity in mice using a variety of behavioral pharmacology methods. In a stress-induced hyperthermia test, 10 days of L-ornithine treatment induced hyperthermia elevation. Pentobarbital-induced sleep time was shortened by the treatment. However, no significant effects were detected in the elevated plus-maze, running wheel, or forced swim tests. These results suggest that long-term treatment with L-ornithine elevates stress sensitivity and arousal level in mice

Simultaneous Two-Photon Absorption of the Thioguanosine Analogue 2′,3′,5′-Tri‑<i>O</i>‑acetyl-6,8-dithioguanosine with Its Potential Application to Photodynamic Therapy

2',3',5'-Tri-O-acetyl-6,8-dithioguanosine (taDTGuo) is an analogue of nucleosides and currently under investigation as a potential agent for photodynamic therapy (PDT). Excitation by simultaneous two-photon absorption of visible or near-infrared light would provide an efficient PDT for deep-seated tumors. The two-photon absorption spectrum of taDTGuo was obtained by optical-probing photoacoustic spectroscopy (OPPAS). A two-photon absorption band corresponding to the S5 ← S0 transition was observed at 556 nm, and the two-photon absorption cross-section σ(2) was determined to be 26 ± 3 GM, which was much larger than that of other nucleobases and nucleosides. Quantum chemical calculations suggested that the large σ(2) value of taDTGuo was responsible for large transition dipole moments and small detuning energy resulting from the thiocarbonyl group at 6- and 8-positions. This is the first report on two-photon absorption spectra and cross-sections of thionucleoside analogues, which could be used to develop a more specific PDT for cancers in deep regions

センゴ ニ オケル キモノ ノ カイリョウ ト アタラシイ キモノ ノ チョウリュウ ザッシ ウツクシイ キモノ ノ ブンセキ カラ

学術論

Verification of numerical code for calculation of characteristics of diffraction and transition radiation

In the present study, we have simulated diffraction and transition radiation using the previously created numerical code. The obtained results are in good agreement with experimental data

End-to-End Joint Target and Non-Target Speakers ASR

This paper proposes a novel automatic speech recognition (ASR) system that can transcribe individual speaker's speech while identifying whether they are target or non-target speakers from multi-talker overlapped speech. Target-speaker ASR systems are a promising way to only transcribe a target speaker's speech by enrolling the target speaker's information. However, in conversational ASR applications, transcribing both the target speaker's speech and non-target speakers' ones is often required to understand interactive information. To naturally consider both target and non-target speakers in a single ASR model, our idea is to extend autoregressive modeling-based multi-talker ASR systems to utilize the enrollment speech of the target speaker. Our proposed ASR is performed by recursively generating both textual tokens and tokens that represent target or non-target speakers. Our experiments demonstrate the effectiveness of our proposed method.Comment: Accepted at Interspeech 202

A new protocol for the preparation of superconducting KBi2

A superconducting KBi2 sample was successfully prepared using a liquid ammonia (NH3) technique. The temperature dependence of the magnetic susceptibility (M/H) showed a superconducting transition temperature (Tc) as high as 3.6 K. In addition, the shielding fraction at 2.0 K was evaluated to be 87%, i.e., a bulk superconductor was realized using the above method. The Tc value was the same as that reported for the KBi2 sample prepared using a high-temperature annealing method. An X-ray diffraction pattern measured based on the synchrotron X-ray radiation was analyzed using the Rietveld method, with a lattice constant, a, of 9.5010(1) Å under the space group of Fd[3 with combining macron]m (face-centered cubic, no. 227). The lattice constant and space group found for the KBi2 sample using a liquid NH3 technique were the same as those reported for KBi2 through a high-temperature annealing method. Thus, the superconducting behavior and crystal structure of the KBi2 sample obtained in this study are almost the same as those for the KBi2 sample reported previously. Strictly speaking, the magnetic behavior of the superconductivity was different from that of a KBi2 sample reported previously, i.e., the KBi2 sample prepared using a liquid NH3 technique was a type-II like superconductor, contrary to that prepared using a high-temperature annealing method, the reason for which is fully discussed. These results indicate that the liquid NH3 technique is effective and simple for the preparation of a superconducting KBi2. In addition, the topological nature of the superconductivity for KBi2 was not confirmed

N-Acetyl-d-Glucosamine-Binding Lectin in Acropora tenuis Attracts Specific Symbiodiniaceae Cell Culture Strains

Many corals establish symbiosis with Symbiodiniaceae cells from surrounding environments, but very few Symbiodiniaceae cells exist in the water column. Given that the N-acetyl-d-glucosamine-binding lectin ActL attracts Symbiodiniaceae cells, we hypothesized that corals must attract Symbiodiniaceae cells using ActL to acquire them. Anti-ActL antibody inhibited acquisition of Symbiodiniaceae cells, and rearing seawater for juvenile Acropora tenuis contained ActL, suggesting that juvenile A. tenuis discharge ActL to attract these cells. Among eight Symbiodiniaceae cultured strains, ActL attracted NBRC102920 (Symbiodinium tridacnidorum) most strongly followed by CS-161 (Symbiodinium tridacnidorum), CCMP2556 (Durusdinium trenchii), and CCMP1633 (Breviolum sp.); however, it did not attract GTP-A6-Sy (Symbiodinium natans), CCMP421 (Effrenium voratum), FKM0207 (Fugacium sp.), and CS-156 (Fugacium sp.). Juvenile polyps of A. tenuis acquired limited Symbiodiniaceae cell strains, and the number of acquired Symbiodiniaceae cells in a polyp also differed from each other. The number of Symbiodiniaceae cells acquired by juvenile polyps of A. tenuis was correlated with the ActL chemotactic activity. Thus, ActL could be used to attract select Symbiodiniaceae cells and help Symbiodiniaceae cell acquisition in juvenile polyps of A. tenuis, facilitating establishment of symbiosis between A. tenuis and Symbiodiniaceae cells

A case of autism spectrum disorder with cleft lip and palate carrying a mutation in exon 8 of AUTS2

We report a patient with autism and cleft lip and palate carrying a de novo heterozygous AUTS2 mutation, c.1464_1467del ACTC (p.Tyr488*). Although the causal relationship between cleft lip and palate and this mutation is unclear, this case report may expand the clinical phenotype of AUTS2 syndrome