23 research outputs found
FOXD1 Promotes Cell Growth and Metastasis by Activation of Vimentin in NSCLC
Background/Aims: Forkhead box D1 (FOXD1) has a well-established role in early embryonic development and organogenesis and functions as an oncogene in several cancers. However, the clinical significance and biological roles of FOXD1 in non-small cell lung cancer (NSCLC) remain largely unknown. Methods: A total of 264 primary NSCLC tissue samples were collected. The expression levels of FOXD1 in these samples were examined by immunohistochemical staining. The expression of FOXD1 was knocked down by lentiviral shRNA. The relative expression of FOXD1 was determined by qRT-PCR, Western blotting and immunofluorescence image. The functional roles of FOXD1 in NSCLC were demonstrated cell viability CCK-8 assay, colony formation, cell invasion and migration assays, and cell apoptosis assay in vitro. In vivo mouse xenograft and metastasis models were used to assess tumorigenicity and metastatic ability. The Chi-square test was used to assess the correlation between FOXD1 expression and the clinicopathological characteristics. Survival curves were estimated by Kaplan-Meier method and compared using the log-rank test. The Cox proportional hazards model was used for univariate and multivariate analyses. Results: We determined that higher levels of FOXD1 were present in NSCLC tissues, especially in metastatic NSCLC tissues. FOXD1 was also higher in all NSCLC cells compared with normal human bronchial epithelial cells. A higher expression level of FOXD1 was associated with malignant behavior and poor prognosis in NSCLC patients. Knockdown of FOXD1 significantly inhibited proliferation, migration, and invasion in vitro and tumor growth and metastasis in vivo, and it increased the apoptosis rates of NSCLC cells. Mechanistic analyses revealed that FOXD1 expressed its oncogenic characteristics through activating Vimentin in NSCLC. Multivariate Cox regression analysis indicated that FOXD1 was an independent prognostic factor both for overall survival (OS) and disease-free survival (DFS) in NSCLC patients. Conclusion: Our results indicated that FOXD1 might be involved in the development and progression of NSCLC as an oncogene, and thereby might be a potential therapeutic target for NSCLC patients
Pemetrexed plus Platinum as the First-Line Treatment Option for Advanced Non-Small Cell Lung Cancer: A Meta-Analysis of Randomized Controlled Trials
To compare the efficacy and toxicities of pemetrexed plus platinum with other platinum regimens in patients with previously untreated advanced non-small cell lung cancer (NSCLC). Methods: A meta-analysis was performed using trials identified through PubMed, EMBASE, and Cochrane databases. Two investigators independently assessed the quality of the trials and extracted data. The outcomes included overall survival (OS), progression-free survival (PFS), response rate (RR), and different types of toxicity. Hazard ratios (HRs), odds ratios (ORs) and their 95% confidence intervals (CIs) were pooled using RevMan software. Results: Four trials involving 2,518 patients with previously untreated advanced NSCLC met the inclusion criteria. Pemetrexed plus platinum chemotherapy (PPC) improved survival compared with other platinum-based regimens (PBR) in patients with advanced NSCLC (HR = 0.91, 95% CI: 0.83–1.00, p = 0.04), especially in those with non-squamous histology (HR = 0.87, 95% CI: 0.77–0.98, p = 0.02). No statistically significant improvement in either PFS or RR was found in PPC group as compared with PBR group (HR = 1.03, 95% CI: 0.94–1.13, p = 0.57; OR = 1.15, 95% CI: 0.95–1.39, p = 0.15, respectively). Compared with PBR, PPC led to less grade 3–4 neutropenia and leukopenia but more grade 3–4 nausea. However, hematological toxicity analysis revealed significant heterogeneities. Conclusion: Our results suggest that PPC in the first-line setting leads to a significant survival advantage with acceptable toxicities for advanced NSCLC patients, especially those with non-squamous histology, as compared with other PRB. PPC could be considered as the first-line treatment option for advanced NSCLC patients, especially those with non-squamous histology
CURRENT STATUS OF RESEARCH ON MECHANICAL VENTILATION STRATEGIES IN PEDIATRIC ACUTE RESPIRATORY DISTRESS SYNDROME
Pediatric acute respiratory distress syndrome (PARDS) is a relatively common critical illness in the pediatric intensive care unit with an incidence of about 3%. It has a high mortality rate; although this number has decreased in the last de-cades, it is still as high as 18%; in some developing countries, it even reached 40%-50% in the last 2-3 years. Optimized mechanical ventilation strategy contributes to an improved prognosis. To this end, this article reviews the current status of research on mechanical ventilation strategies in PARDS
Pt Catalyzed Hydrosilylation of Fluorinated Phenoxy-Imine Ligands with Hydrosilanes
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Evaluation of methanogenic microbial electrolysis cells under closed/open circuit operations
Reconstruction of large unilateral hemi-facial scar contractures with supercharged expanded forehead flaps based on the anterofrontal superficial temporal vessels
Retraction Note: The HOTAIRM1/miR-107/TDG axis regulates papillary thyroid cancer cell proliferation and invasion
Evaluation of methanogenic microbial electrolysis cells under closed/open circuit operations
<p>The present study investigated the independent roles of biofilm and external power supply for volatile fatty acids degradation and methane production in the microbial electrolysis cell. Reactors were operated in fed-batch mode in the presence of graphite felt (GF) or titanium rod (Ti) as electrodes, in open circuit (OC, without applied voltage) or closed circuit (CC) conditions, i.e. R1 (Ti + CC), R2 (GF + CC) and R3 (GF + OC). The first-order kinetic analysis of acetate degradation indicated that the presence of GF biofilm and applied voltage in R2 improved the degradation rate of acetate by 23% as compared to R1, while it was only a 7% increment in R3 with GF biofilm. The degradation of butyrate was accelerated by 12% in the first 24 h, whereas there was no enhancement of the propionic acid digestion. Generally, methane yields from the three reactors followed the sequence: R2 > R1 > R3, indicating the positive effect of external power supply on methane generation. High-throughput sequencing revealed that <i>Geobacter</i> sp. could be enriched on conductive GF even without electric stimulation. The clustered <i>Geobacter</i> and <i>Methanosarcina</i> in R2 presented the potential to promote interspecies electron transfer and accelerate substrate utilization and methane production.</p