GENETIC IN CANCER: RB1 MUTATIONS IN RETINOBLASTOMA CASES

Retinoblastoma (RB) is a uncommon childhood malignant ailment induced by means of the biallelic inactivation of RB1 gene. Early analysis and identification of carriers of heritable RB1 mutations can enhance sickness effect and management. In this study, mutational evaluation used to be performed on fifty-nine matched tumor and peripheral blood samples from 18 bilateral and forty one unilateral unrelated RB instances by means of a combinatorial strategy of Multiplex Ligation-dependent Probe Amplification (MLPA) assay, deletion screening, direct sequencing, replica wide variety gene dosage evaluation and methylation assays. Screening of each blood and tumor samples yielded a mutation detection charge of 94.9% (56/59) whilst solely 42.4% (25/59) of mutations had been detected if blood samples on my own had been analyzed. Biallelic mutations had been discovered in 43/59 (72.9%) of tumors screened. There have been three instances (5.1%) in which no mutations may want to be detected and germline mutations have been detected in 19.5% (8/41) of unilateral cases. A whole of sixty one factor mutations have been identified, of which 10 had been novel. There was once a excessive incidence of in the past stated recurrent mutations, taking place at 38.98% (23/59) of all cases. Of pastime had been three instances of mosaic RB1 mutations detected in the blood from sufferers with unilateral retinoblastoma. Our findings additionally underscore the significance of genetic checking out in aiding individualized disorder administration plans for sufferers and asymptomatic household individuals carrying low-penetrance, germline mosaicism or heritable unilateral mutational phenotypes

The Relationship Between Husband Support and Behavior of Pregnant Teenagers to Face Pregnancy During the Covid-19 Pandemic in Gombong, Kebumen, Indonesia

Background: Age influences pregnancy and childbirth. Pregnant women aged less than 20 years old or pregnant adolescents are not physically and mentally ready to cope with pregnancy or childbirth. The husband's support highly determines the health status of the mother. This research aimed to determine the relationship between husband support and the behavior of pregnant adolescents to face pregnancy during the COVID-19 pandemic in the Gombong District, Kebumen Regency. Methods: This study used a cross-sectional approach. The population was all pregnant women aged less than 20 years in the Gombong District, Kebumen Regency. It used a saturated sampling technique involving 256 people. Data were analyzed using univariate and bivariate analysis to answer the percentage and the Chi-Square test to answer the research hypothesis. Results: The study showed that 130 (50.8%) husbands did not support the mothers, and 126 (49.2%) husbands supported the mothers. The result also showed that 116 (45.3%) of the pregnant women showed positive behavior, while140 (54.7%) pregnant women showed negative behavior. The chi-square test found a p-value of 0.005, which proved a significant relationship between the husband's support and the behavior of pregnant adolescents to overcome pregnancy during the covid-19 pandemic. Conclusion: Pregnant teenagers with husband support will prepare for pregnancy and childbirth programs more maturely to ensure the safety of both mother and fetus

Edukasi Pengenalan Seks Dini Pada Komunitas Anak Di Rumah Singgah Kota Bengkulu

Sexual violence is still high, which is proof of low parental knowledge to apply parenting concepts and low child knowledge in general. For decreasing sexual violence, early education about sex is a solution to improving children's knowledge and a preventive measure to protect them. Community service is proposed for the children's community under KCS Odot, Bengkulu City, Indonesia. respondents to reduce the case by proposing improved children's sex education knowledge. According to the results of community service in the KCS Odot child community in Bengkulu city, children had good knowledge among 53 respondents (60%), and low knowledge among 36 respondents (40%). According to the findings, majority  of participants had a good level of early sexual education

Social-Economic and Children’s Nutritional Status in Bengkulu Province

This study aims to determine the factors related to the nutritional status of children in Bengkulu Province due to COVID-19 that affect economic conditions and make the healthy situation worse than before, especially child nutrition. The method used is a cross-sectional study conducted through an online questionnaire. The results of this study indicate that the factors that are significantly related to the nutritional status of children are; financially inadequate (AOR = 2.32; 95% CI: 1.50 to 3.60, P<0.0001), employed (AOR = 3.03; 95% CI: 193 to 4.76, P< 0.0001), living in urban areas (AOR = 1.59; 95% CI: 1.03 – 2.43, P: 0.034), local ethnicity (AOR = 1.72; 95% CI: 1.11 to 2, 68, P: 0.016) and low-income family support (AOR = 2.10; 95% CI: 1.34 to 3.28 < P<0.0001). In conclusion, financial condition, employment status, place, ethnicity, and family are related to the nutritional status of children in Bengkulu Province. Keywords: Financial Condition, Employment, Children's Nutritional Statu

Occupational Safety Practice of Hazardous Health-care Waste Management in Bengkulu City

Background: Facing many patients while the workers were limited prompted health workers to make a quick decision regarding health-care waste. High workload pressured health workers and put them at risk of getting diseases than others, especially diseases from medical waste that they took care. Besides, as health workers, they should be clean from bacteria or viruses that can transmit  diseases to their patients. This research aimed to investigate the factors associated with occupational safety practice of hazardous health-care waste management in Bengkulu City. Method: This cross-sectional study used a stratified random sampling technique to select 230 respondents who work as health workers to respond to a questionnaire interview. The data were analyzed using multivariate logistic regression to find the association between the outcomes and the independent variables. Results: The prevalence of   poor  occupational safety practice of hazardous health-carewaste management was 35.21 % (95% CI = 29.27-41.66).  The factors significantly associated with occupational safety practice of hazardous health-care waste management were; gender (p-value < 0.001), department/unit (p-value < 0.001), knowledge (p-value < 0.001), and spirituality (p-value  < 0.001). Conclusion:  gGnder, department/unit, knowledge, and spirituality were associated with occupational safety practice of hazardous health-carewaste management in Bengkulu City

Pelatihan Prosedur Keselamatan Bagi Pemandu Pariwisata Air Terjun Trisakti Desa Belitar Seberang

Indonesia adalah negara yang mempunyai ribuan pulau dengan kekayaan alam dan keaneka ragaman budaya yang menarik dan memiliki ciri khas tersendiri bagi siapa saja yang melihatnya. Indonesia memiliki obyek wisata alam yang terkenal di seluruh dunia, baik itu pantai, pegunungan, hutan wisata, air terjun, wisata goa yang potensi besar untuk menjadi salah satu negara yang menjadi tujuan wisata. Tempat wisata identik dengan bersenang-senang, namun kegiatan wisata ini juga tentunya memiliki berbagai macam resiko, seperti resiko kejadian gawat atau kecelakaan yang menimpa pelaku pariwisata baik itu pekerja maupun wisatawan. Adapun kejadian gawat darurat yang dialami di tempat wisata seperti kehilangan kesadaran karena terseret arus/tenggelam, luka, patah tulang terkilir, dislokasi lutut dan sebagainya. Dalam kejadian ini pelaku pariwisata baik itu wisatawan, penyedia jasa, pendukung wisata, pemerintah, masyarakat lokal merupakan first responder yang termasuk sebagai orang awam atau masyarakat umum. Secara spontan sebagian dari mereka melakukan pertolongan terhadap korban sesuai dengan pengetahuannya (Pro Emergency, 2011).  Pramuwisata adalah seseorang yang dibayar untuk menemani wisatawan dalam perjalanan mengunjungi, melihat, menyaksikan objek dan atraksi wisata yang menurut wisatawan pramuwisata adalah seseorang yang bekerja pada suatu biro perjalanan atau suatu kantor pariwisata (tourism office) yang bertugas memberikan informasi, petunjuk secara langsung kepada wisatawan sebelum dan selama perjalanan

Pengaruh Roleplay Pendidikan Kesehatan Tentang Pertolongan Pertama Pada Kecelakaan (P3K) Terhadap Pengetahuan Pemandu Parawisata Di Desa Blitar Sebrang Kabupaten Rejang Lebong Tahun 2022

Background. The provision of first aid must be done quickly and accurately by using the facilities and infrastructure available at the scene. A first aid action that is done properly will reduce disability or suffering and even save the victim from death, but if the first aid action is not carried out properly it can even worsen the accident and even cause death, a tour guide must be able to provide security and safety to every tourist who visits. Health education is all activities to provide and improve the knowledge, attitudes, practices of individuals, groups or communities in maintaining and improving their own health. Purpose of this study was to determine the effect of Health Education Roleplay on First Aid in Accidents (P3K) on the Knowledge of Tourist Guides in Blitar Sebrang Village, Rejang Lebong Regency. Methods This research is analytical research with Pre Experimental Design (The One Group Pre-Test-Post Test Design). The sampling technique used stratified random sampling. The number of respondents is 35 people. Results of statistical tests before and after the intervention of health education about first aid in accidents (P3K) on the knowledge of tour guides showed a significant effect with the P-Value = 0.000 less than 0.05. Conclusion There is an Influence of Health Education Roleplay on First Aid in Accidents (P3K) on Knowledge of Tourist Guides in Blitar Sebrang Village, Rejang Lebong Regency. &nbsp

Edukasi Dan Pemeriksaan Penyakit Kusta Dan Frambusia Di RT 27 Dusun Besar Wilayah Kerja Puskesmas Jembatan Kecil

Leprosy (morbus hansen) is a chronic chronic infectious disease caused by Mycobacterium leprae (M.leprae) which attacks almost all organs of the body, especially the peripheral nerves and skin and other organs such as the oral mucosa, upper respiratory tract, reticuloendothelial system, eyes. , muscles and bones except the central nervous system. Yaws is a chronic infectious disease caused by Treponema pallidum subspecies pertenue which occurs in the tropics, especially in densely populated areas, low socio-economic, and poor hygiene). Measurement activities with the theme Education and Examination of Leprosy and Yaws in Rt 27 Dusun Besar Working Area of the Jembatan Kecil Health Center with the aim of increasing knowledge and early detection of Leprosy and Yaws. The community service activities that have been carried out are considered very important so that the community can recognize and detect leprosy and yaws early and get treatment immediately. Community service participants were able to briefly explain leprosy and yaws. The community service participants were enthusiastic about the activities carried out to increase knowledge in the form of education about leprosy and yaws. The participants were very happy to participate in the examination of leprosy and yaw

Faktor – faktor yang berhubungan dengan gejala depresi di kalangan perempuan yang melakukan pernikahan dini

Background: In general, the role of the mother in family life is to be responsible for taking care of the family. Mother has to handle all aspects of household management, including childcare, money management, food preparation, etc. Requires mental readiness to carry out its role which has the potential to cause depression.Purpose: To identify factors correlated with depression symptoms among women in early married.Method: Using a stratified random sampling procedure, 488 participants were selected for the interview and questionnaire in this cross-sectional study. Married women experience pressure because of the condition of their marriage, especially those who marry at a young age. The dependent variable, depression, was divided into two groups: the group with depressive symptoms with a score range of >60, and the group without depressive symptoms with a score range of 0 to 60. The fourteen independent variables were age of marriage, religion, education, ethnicity, area of residence in Bengkulu Province, occupation, place of residence, household income per month, poor living conditions, social influence on depression, family influence on depression, and stress. This study also includes three types of tests: univariate, logistic regression, and multiple logistic regression. Logistic regression and multiple logistic regression were used as data analysis to find the relationship between the independent factors and the dependent variable.Results: The prevalence of depression was 77.87% (95% CI: 73.95-81.34). The factors that are significantly related to depression are; 60, dan mereka yang tidak memiliki gejala depresi dengan rentang skor 0 hingga 60. Empat belas variabel independen adalah usia pernikahan, agama, pendidikan, etnis, wilayah tempat tinggal di Provinsi Bengkulu, pekerjaan, tempat tinggal, pendapatan rumah tangga per bulan, kondisi tempat tinggal yang buruk, pengaruh komunitas terhadap depresi, pengaruh keluarga terhadap depresi, dan stres. Penelitian ini juga mencakup tiga jenis uji yang berbeda: univariat, regresi logistik, dan regresi logistik berganda. Regresi logistik dan regresi logistik berganda digunakan sebagai data analisis untuk menemukan hubungan antara faktor independen dan variabel dependen.Hasil: Prevalensi depresi adalah 77,87% (95% CI: 73,95-81,34). Faktor-faktor yang secara signifikan berhubungan dengan depresi adalah; < 18 tahun (AOR = 6,19; 95% CI: 2,98 - 12,88), memiliki pendapatan rumah tangga < Rp. 122.72.000 (OR = 7,01; 95% CI: 3,62 - 13,57), kondisi tempat tinggal yang buruk (OR = 2,16; 95% CI: 1,11 - 4,18), memiliki pengaruh komunitas terhadap depresi (OR = 3,68; 95% CI: 1,91 - 7,06) dan mengalami stres (OR = 9,85; 95% CI: 5,24 - 18,24). Simpulan: Usia pernikahan, pendapatan rumah tangga, kondisi kehidupan yang buruk, depresi di masyarakat, dan stres semuanya berhubungan dengan depresi di antara perempuan yang sudah menikah di Provinsi Bengkulu