Assessment of Development of Yuksom Gram Panchayat Unit in Sikkim using SWOT Model

SWOT model is a technique to appraise strategies for rural development. This study aims to apply this model to examine the development of Yuksom Gram Panchayat Unit (GPU) of West district of Sikkim, India. To accomplish this analysis, internal factor evaluation (IFE) matrix and external factor evaluation matrix (EFE) were prepared to identify the critical and less important factors for development. Finally, a framework for strategy has been formulated by linking ‘strength-opportunity’ (SO) and ‘weakness-threat’ (WT) aspects. Results show mountain environment sustainability as the most agreed one (SO) and on the other hand, implementation of ‘land bank scheme’ and microfinance (WT) as the alternate planning strategies for the development of the Yuksom are

Population Growth, Infrastructural Development and Exposure to Seismic Hazard: Challenges of East Sikkim

A comprehensive, analytical and numerical application of population growth, rapid building construction and associated risk is presented in the paper. East district of Sikkim (27°08?N to 27°23?N and 88°25?E to 88°45?E) constitutes 954 sq km. area and it is the most developed district in Sikkim. Unstable geostratigrphical settings along with unscientific use of heavy building materials further exaggerate risk in the fragile Eastern Himalayan landscape. DOI: 10.17762/ijritcc2321-8169.150710

Economy and Social Development of Rural Sikkim

The tiny Himalayan state of Sikkim is well known for its multi-cultural and multi-ethnic identity. There is a political and historical debate regarding the identity of communities in Sikkim. Lepchas are considered as original inhabitants of Sikkim. Currently, Lepcha, Bhutia and Limbu are recognised as minor communities and have Schedule Tribes (ST) status in the state. Individual community concentration is mainly found in North and West Sikkim. Lepcha-Bhutias are found mainly in North Sikkim whereas Limbus are concentrated in West Sikkim. Community concentration is profound in rural areas. Gyalshing sub-division of West Sikkim has been selected for the present study. Purpose of this study is to investigate the Gram Panchayat Unit (GPU) level economy and social development of the rural areas based mainly on secondary sources of information. A field survey was also conducted to interact with the local people. Findings suggest that education and population density are the key determinants for GPU level disparity in social development of the study area. It has been found that the economy is primarily agriculture based and fully organised by organic farming system. Recently, homestay (eco)tourism business has been started here like other parts of Sikkim

TREND OF URBANISATION AND GROWTH OF SMALL TOWNS IN BARDDHAMAN DISTRICT, WEST BENGAL, INDIA

As per the census of India 2011, Barddhaman district (22o56' N to 23o53'N and 86o48' E to 88o25' E) holds 4th position in percentage share of urban population in West Bengal, India. The district shows an increase in rate of urbanisation from 1971-2011. Level of urbanisation is high than the state and national averages. There exists wide intra district variation in level of urbanisation. Interestingly, much of the share comes from Western Barddhaman, comprising Asansol and Durgapur subdivisions. Town spacing is also low here, indicating high concentration. The present work intends to study trend of urbanisation and growth of small towns in Barddhaman district, West Bengal, India. Census of India data (1971 to 2011) have been used for the analysis. There is an uneven development of the small towns and the number of them obviously high in Western one than the Eastern part

Perspective on Large Cardamom Cultivation and its Challenges in West Sikkim, India

Sikkim is the smallest mountainous state in North-East India. Agriculture is the backbone of rural Sikkim. It contributes 16% of the Gross Domestic Product (GDP) of the State and supports more than 64% of the population. In 2015, Sikkim Organic Mission was launched, and the focus was shifted from subsistence to commercial agriculture. Farmers shifted their choice from food crop cultivation to large cardamom cultivation for high-profit making purposes. Presently, cardamom cultivation in West Sikkim suffers from challenges like maintaining the quality of the product and lack of scientific technique to tackle the situation

Dopamine induces functional extracellular traps in microglia

Dopamine (DA) plays many roles in the brain, especially in movement, motivation, and reinforcement of behavior; however, its role in regulating innate immunity is not clear. Here, we show that DA can induce DNA-based extracellular traps in primary, adult, human microglia and BV2 microglia cell line. These DNA-based extracellular traps are formed independent of reactive oxygen species, actin polymerization, and cell death. These traps are functional and capture fluorescein (FITC)-tagged Escherichia coli even when reactive oxygen species production or actin polymerization is inhibited. We show that microglial extracellular traps are present in Glioblastoma multiforme. This is crucial because Glioblastoma multiforme cells are known to secrete DA. Our findings demonstrate that DA plays a significant role in sterile neuro-inflammation by inducing microglia extracellular traps

Pasteurella multocida toxin- induced osteoclastogenesis requires mTOR activation

Background: Pasteurella multocida toxin (PMT) is a potent inducer of osteoclast formation. Pigs suffering from an infection with toxigenic Pasteurella multocida strains develop atrophic rhinitis characterised by a loss of turbinate bones and conchae. However, on the molecular level the process of bone loss remains largely uncharacterised. Results: Recently it was found that PMT activates the serine/threonine kinase mammalian target of rapamycin (mTOR) in fibroblasts. Using RAW264.7 macrophages, we investigated the role of the mTOR complex 1 (mTORC1) in PMT-mediated osteoclast formation. PMT induces the differentiation of RAW264.7 macrophages into multinucleated, tartrate resistant acid phosphatase (TRAP) positive osteoclasts that are capable to resorb bone. In the presence of the mTORC1 inhibitor rapamycin, PMT was significantly less able to induce the formation of TRAP-positive osteoclasts. Accordingly, the resulting resorption of bone was strongly reduced. A major target of mTOR is the 70 kDa ribosomal protein S6 kinase 1 (p70 S6K1). Activated p70 S6K1 decreases the expression of programmed cell death protein 4 (PDCD4), a negative transcriptional regulator of osteoclastogenesis, at the protein and gene level. Ultimately this results in the activation of c-Jun, a component of the activator protein 1 (AP-1) complex, which is a major transcription factor for the induction of osteoclast-specific genes. We now demonstrate that c-Jun and its downstream target, the osteoclast-specific bone degrading protease cathepsin K, are upregulated upon PMT treatment in an mTOR-dependent manner. Conclusions: Activation of mTOR signalling plays a central role in the formation of osteoclasts through the bacterial toxin PMT. On the molecular level, PMT-induced activation of mTOR leads to down regulation of PDCD4, a known repressor of AP-1 complex, culminating in the activation of c-Jun, an essential transcription factor for triggering osteoclastogenesis

Shielding in whole brain irradiation in the multileaf collimator era: Dosimetric evaluation of coverage using SFOP guidelines against in-house guidelines

Aim : Compare the planning target volume (PTV) coverage in three different shielding techniques in cranial irradiation. Settings and Design : Tertiary care center, prospective study. Materials and Methods : The whole brain and meninges were contoured in ten planning CT scans, and expanded by 5 mm for the PTV. Shielding was designed using the French Society of Pediatric Oncology (SFOP) guidelines (SFOP plan), in-house recommendation (with 1 cm margin from the orbital roof and sphenoid wing) on a igitally Reconstructed Radiograph (DRR) and a third plan was generated using a 3D conformal radiation technique (3DCRT). The coverage of the PTV was noted using the isodose covering 95% of the PTV(D95), minimum dose within the PTV(D min ), and maximum dose within the PTV(D max ). The location of PTV not covered by the 95% isodose curve was noted. The median dose and maximum dose (D max ) to both eyes and maximum dose D max for the lens were noted. Statistical Analysis : General linear model method repeated the measure of analysis of variance test (ANOVA). Results : PTV coverage was significantly poorer in the SFOP and in-house plans as compared to 3DCRT plan (P=0.04). Median volume of PTV not covered by 95% isodose curve was 4.18 cc, 1.01 cc, and 0 cc in SFOP, in-house, and 3DCRT plan, respectively. Conclusions : In the absence of volumetric planning techniques, SFOP guidelines lead to inadequate coverage and the in-house method is recommended

A network-based comparative framework to study conservation and divergence of proteomes in plant phylogenies

Comparative functional genomics offers a powerful approach to study species evolution. To date, the majority of these studies have focused on the transcriptome in mammalian and yeast phylogenies. Here, we present a novel multi-species proteomic dataset and a computational pipeline to systematically compare the protein levels across multiple plant species. Globally we find that protein levels diverge according to phylogenetic distance but is more constrained than the mRNA level. Module-level comparative analysis of groups of proteins shows that proteins that are more highly expressed tend to be more conserved. To interpret the evolutionary patterns of conservation and divergence, we develop a novel network-based integrative analysis pipeline that combines publicly available transcriptomic datasets to define co-expression modules. Our analysis pipeline can be used to relate the changes in protein levels to different species-specific phenotypic traits. We present a case study with the rhizobia-legume symbiosis process that supports the role of autophagy in this symbiotic association

Pasteurella multocida toxin- induced osteoclastogenesis requires mTOR activation

Background Pasteurella multocida toxin (PMT) is a potent inducer of osteoclast formation. Pigs suffering from an infection with toxigenic Pasteurella multocida strains develop atrophic rhinitis characterised by a loss of turbinate bones and conchae. However, on the molecular level the process of bone loss remains largely uncharacterised. Results Recently it was found that PMT activates the serine/threonine kinase mammalian target of rapamycin (mTOR) in fibroblasts. Using RAW264.7 macrophages, we investigated the role of the mTOR complex 1 (mTORC1) in PMT-mediated osteoclast formation. PMT induces the differentiation of RAW264.7 macrophages into multinucleated, tartrate resistant acid phosphatase (TRAP) positive osteoclasts that are capable to resorb bone. In the presence of the mTORC1 inhibitor rapamycin, PMT was significantly less able to induce the formation of TRAP-positive osteoclasts. Accordingly, the resulting resorption of bone was strongly reduced. A major target of mTOR is the 70 kDa ribosomal protein S6 kinase 1 (p70 S6K1). Activated p70 S6K1 decreases the expression of programmed cell death protein 4 (PDCD4), a negative transcriptional regulator of osteoclastogenesis, at the protein and gene level. Ultimately this results in the activation of c-Jun, a component of the activator protein 1 (AP-1) complex, which is a major transcription factor for the induction of osteoclast-specific genes. We now demonstrate that c-Jun and its downstream target, the osteoclast-specific bone degrading protease cathepsin K, are upregulated upon PMT treatment in an mTOR-dependent manner. Conclusions Activation of mTOR signalling plays a central role in the formation of osteoclasts through the bacterial toxin PMT. On the molecular level, PMT-induced activation of mTOR leads to down regulation of PDCD4, a known repressor of AP-1 complex, culminating in the activation of c-Jun, an essential transcription factor for triggering osteoclastogenesis