35 research outputs found
Increasing Fatigue LIfe of 09Mn2Si Steel by means of High-Temperature Multistep Helical Rolling
The effect of high temperature helical rolling (HR) on structure and fatigue life of 09Mn2Si pipe steel has been studied. With the use of transmission electron microscopy there was revealed that rolling gives rise to refinement of ferrite grains and cracking (fracturing) of cementite plates within the pearlite phase. The effect manifests itself to the greatest extent in the surface layer where due to the rolling the level of plastic deformation was the highest. Data of microhardness measurements confirms the gradient pattern of strain hardening over the cross section during the HR occurs while the most intensive microhardness increasing take place at the depth of up to 3 mm. According to the mechanical testing results the helical rolling of 09Mn2Si steel gives rise to increasing the level of deforming stress at the yield plateau as well as the proportionality limit with a general decrease in the relative elongation. At the same time, despite the strain hardening resulting from the helical rolling the mechanisms of plastic deformation which manifest themselves in the form of parabolic hardening with a smooth decrease in the flow stress level after neck formation are preserved in the steel. During the cyclic tension the number of cycles prior to failure increases from 2.5 to 3.8 times that depends on the location of specimens' cutting from the rolled rod. The highest improvement in fatigue fracture resistance is registered for specimens cut out from the core of the rolled rods
Increasing Fatigue LIfe of 09Mn2Si Steel by means of High-Temperature Multistep Helical Rolling
The effect of high temperature helical rolling (HR) on structure and fatigue life of 09Mn2Si pipe steel has been studied. With the use of transmission electron microscopy there was revealed that rolling gives rise to refinement of ferrite grains and cracking (fracturing) of cementite plates within the pearlite phase. The effect manifests itself to the greatest extent in the surface layer where due to the rolling the level of plastic deformation was the highest. Data of microhardness measurements confirms the gradient pattern of strain hardening over the cross section during the HR occurs while the most intensive microhardness increasing take place at the depth of up to 3 mm. According to the mechanical testing results the helical rolling of 09Mn2Si steel gives rise to increasing the level of deforming stress at the yield plateau as well as the proportionality limit with a general decrease in the relative elongation. At the same time, despite the strain hardening resulting from the helical rolling the mechanisms of plastic deformation which manifest themselves in the form of parabolic hardening with a smooth decrease in the flow stress level after neck formation are preserved in the steel. During the cyclic tension the number of cycles prior to failure increases from 2.5 to 3.8 times that depends on the location of specimens' cutting from the rolled rod. The highest improvement in fatigue fracture resistance is registered for specimens cut out from the core of the rolled rods
Possibilities for predicting ventricular tachyarrhythmias in patients with heart failure with reduced ejection fraction based on surface electrocardiography. First results from a single-center prospective study
According to current clinical guidelines, the risk of life-threatening ventricular tachyarrhythmias (VTAs) in patients with heart failure (HF) is determined by left ventricular ejection fraction (LVEF). The available clinical and experimental data indicate the imperfection of this one-factor approach, which specifies the need to search for new predictors of VTAs. In this prospective study, we performed a comparative analysis of surface electrocardiographic parameters in HF patients with LVEF β€35% without syncope or sustained ventricular arrhythmias in history, who were implanted with cardioverter defibrillator as a primary prevention of sudden cardiac death. During the two-year follow-up, the primary endpoint (new-onset persistent VTA episode, or VTA/ventricular fibrillation that required electrotherapy) was recorded in 42 patients (25,5%). The secondary endpoint (an increase in LVEF by 5% or more of the initial level against the background of cardiac resynchronization therapy) was more often recorded in the group of patients without VTAs (41 (33%) vs 4 (9,5%), p=0,005). The studied cohort of patients was characterized by a left axis deviation (72%), LV hypertrophy signs (84%), impaired intra-atrial (P wave duration of 120 (101-120) ms) and intraventricular conduction (QRS duration of 140 (110-180) ms), ventricular electrical systole prolongation (QTcor β 465 (438-504) ms). Differences between the groups divided depending on reaching the primary endpoint in terms of the Cornell product, Cornell voltage index and ICEB, as well as the detection rate of complete left bundle branch block morphology had levels of significance close to critical (p=0,09; p=0,05; p=0,1; p=0,09, respectively). The multivariate predictive model included following factors: Cornell product, Tp-Te/ QRS, P wave duration (diagnostic efficiency of the model was 60%: sensitivity, 61,1%, specificity, 59,6%; p=0,007)
EFFECT OF UROKINASE GENE-KNOCKOUT ON GROWTH OF MELANOMA IN EXPERIMENT
The purpose of the study was to reveal special features of the Π16/F10 melanoma growth in urokinase (uPA) geneΒ knockout mice with and without chronic neurogenic pain (CNP). Material and methods. The study included male andΒ female Π‘57ΠL/6 mice (n = 102) and C57BL/6-Plautm1.1BugThisPlauGFDhu/GFDhu mice with uPA gene knockoutΒ (n = 48). Mice of the main subgroups underwent subcutaneous transplantation of Π16/F10 melanoma 2 weeks afterΒ bilateral ligation of sciatic nerves (CNP model); mice of the same strain with standard melanoma transplantation servedΒ as controls. Results and discussion. Survival of uPA gene knockout mice did not differ from that of normal animals βΒ 1.5 times higher in females than in males (p < 0.05), with melanoma onset in gene-deficient mice a week earlier. TheΒ dynamics of tumor growth had pronounced gender differences: in females, the tumor did not grow and its maximalΒ volume prior to death was 1.0 cm3, while tumors in males were characterized by an active growth with two peaks ofΒ volume increase (weeks 2 and 4). Melanoma was weakly metastatic β solitary metastases to the lungs (in females) orΒ no metastases, but pulmonary and heart hemorrhages were noted (in males). CNP decreased the survival of uPA geneΒ knockout females, as well as of normal animals, but did not influence the survival of males; primary tumors in genedeficientΒ mice appeared a few days later than in controls but their growth was more intense, with diminished genderΒ differences. Increased metastasis was manifested by the initiation of metastatic lesions to the lungs and liver in males,Β with maintained pulmonary hemorrhages, and by increased number of metastatic foci in the lungs together with theΒ appearance of pulmonary hemorrhages in females. Conclusions. The influence of uPA gene knockout on the courseΒ of Π16/F10 melanoma differs in male and female mice. CNP enhances malignant tumor growth, diminishing genderΒ differences, and activates melanoma metastasis
ΠΠ½ΡΠ΅Π½ΡΠΈΠ²Π½ΠΎΡΡΡ Ρ Π΅ΠΌΠΈΠ»ΡΠΌΠΈΠ½Π΅ΡΡΠ΅Π½ΡΠΈΠΈ, ΡΠΎΡΡΠΎΡΠ½ΠΈΠ΅ Π°Π½ΡΠΈΠΎΠΊΡΠΈΠ΄Π°Π½ΡΠ½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΡ ΠΈ ΠΎΠΊΠΈΡΠ»ΠΈΡΠ΅Π»ΡΠ½Π°Ρ ΠΌΠΎΠ΄ΠΈΡΠΈΠΊΠ°ΡΠΈΡ Π±Π΅Π»ΠΊΠΎΠ² ΠΏΠ»Π°Π·ΠΌΡ ΠΊΡΠΎΠ²ΠΈ ΠΏΡΠΈ ΡΠ°Π·Π²ΠΈΡΠΈΠΈ ΡΠ΅ΡΠΈΠ΄ΠΈΠ²Π° ΡΠ°ΠΊΠ° ΡΠΈΡΠ½ΠΈΠΊΠΎΠ²
The state of antioxidant system (the activity of catalase and ceruloplasmine), the intensity of chemiluminescence, and oxidative modification of proteins were studied in blood plasma of 61 patients with ovarian cancer (cystadenocarcinoma) IIIβIV stage in state of remission and ones with a local recurrent tumor. The dynamics of these indices was analyzed in dependence of recurrent tumor vascularization. The statistically significant changes of the activity of some links of antioxidant system were found, as well as chemiluminescence intensity increase in blood plasma. The level of oxidative modificated protein molecules was raised, the most expressed for products of the main character (530 nm). The dynamics of level of carbonyl derivatives of the neutral and main character (370 and 530 nm) was opposite directed during intensification of recurrent tumour vascularization and increase in speed of blood-groove in a recurrent tumorΠ£ 61 Π±ΠΎΠ»ΡΠ½ΠΎΠΉ ΡΠ°ΠΊΠΎΠΌ ΡΠΈΡΠ½ΠΈΠΊΠΎΠ² (ΡΠΈΡΡΠ°Π΄Π΅Π½ΠΎΠΊΠ°ΡΡΠΈΠ½ΠΎΠΌΠ°) IIIβIV ΡΡΠ°Π΄ΠΈΠΉ Π² ΡΠΎΡΡΠΎΡΠ½ΠΈΠΈ ΡΠ΅ΠΌΠΈΡΡΠΈΠΈ ΠΈ Ρ Π»ΠΎΠΊΠ°Π»ΡΠ½ΡΠΌ ΡΠ΅ΡΠΈΠ΄ΠΈΠ²ΠΎΠΌ ΠΈΡ- ΡΠ»Π΅Π΄ΠΎΠ²Π°Π½Ρ Π² ΠΏΠ»Π°Π·ΠΌΠ΅ ΠΊΡΠΎΠ²ΠΈ ΠΈΠ½ΡΠ΅Π½ΡΠΈΠ²Π½ΠΎΡΡΡ Ρ
Π΅ΠΌΠΈΠ»ΡΠΌΠΈΠ½Π΅ΡΡΠ΅Π½ΡΠΈΠΈ, ΡΠΎΡΡΠΎΡΠ½ΠΈΠ΅ ΡΡΠ΄Π° Π·Π²Π΅Π½ΡΠ΅Π² Π°Π½ΡΠΈΠΎΠΊΡΠΈΠ΄Π°Π½ΡΠ½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΡ (Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΠΊΠ°ΡΠ°Π»Π°Π·Ρ, ΡΠ΅ΡΡΠ»ΠΎΠΏΠ»Π°Π·ΠΌΠΈΠ½Π°) ΠΈ ΡΡΠΎΠ²Π΅Π½Ρ ΠΎΠΊΠΈΡΠ»ΠΈΡΠ΅Π»ΡΠ½ΠΎΠΉ ΠΌΠΎΠ΄ΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ Π±Π΅Π»ΠΊΠΎΠ² ΠΎΠ±ΡΠ΅ΠΏΡΠΈΠ½ΡΡΡΠΌΠΈ ΡΠΏΠ΅ΠΊΡΡΠΎΡΠΎΡΠΎΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΠΌΠ΅ΡΠΎΠ΄Π°ΠΌΠΈ. ΠΡΠΎΠ°Π½Π°Π»ΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Π° Π΄ΠΈΠ½Π°ΠΌΠΈΠΊΠ° ΠΈΠ·ΡΡΠ΅Π½Π½ΡΡ
ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Π΅ΠΉ Π² ΠΊΡΠΎΠ²ΠΈ Π±ΠΎΠ»ΡΠ½ΡΡ
Ρ ΡΠ΅ΡΠΈΠ΄ΠΈΠ²ΠΎΠΌ Π² Π·Π°Π²ΠΈΡΠΈΠΌΠΎΡΡΠΈ ΠΎΡ ΠΈΠ½ΡΠ΅Π½ΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΊΡΠΎΠ²ΠΎΡΠΎΠΊΠ° Π² ΡΠ΅ΡΠΈΠ΄ΠΈΠ²Π½ΠΎΠΉ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ. ΠΠ±Π½Π°ΡΡΠΆΠ΅Π½ΠΎ Π΄ΠΎΡΡΠΎΠ²Π΅ΡΠ½ΠΎΠ΅ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π½Π΅ΠΊΠΎΡΠΎΡΡΡ
Π·Π²Π΅Π½ΡΠ΅Π² Π°Π½ΡΠΈΠΎΠΊΡΠΈΠ΄Π°Π½ΡΠ½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΡ, ΡΡΠΈΠ»Π΅Π½ΠΈΠ΅ Ρ
Π΅ΠΌΠΈΠ»ΡΠΌΠΈΠ½Π΅ΡΡΠ΅Π½ΡΠΈΠΈ ΠΏΠ»Π°Π·ΠΌΡ ΠΊΡΠΎΠ²ΠΈ. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ ΡΡΠ΅ΠΏΠ΅Π½ΠΈ ΠΎΠΊΠΈΡΠ»Π΅Π½Π½ΠΎΡΡΠΈ Π±Π΅Π»ΠΊΠΎΠ²ΡΡ
ΠΌΠΎΠ»Π΅ΠΊΡΠ», Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΠΎΠ΅ Π΄Π»Ρ ΠΏΡΠΎΠ΄ΡΠΊΡΠΎΠ² ΠΎΡΠ½ΠΎΠ²Π½ΠΎΠ³ΠΎ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠ° (530 Π½ΠΌ). ΠΠΎ ΠΌΠ΅ΡΠ΅ ΠΏΡΠΎΠ³ΡΠ΅ΡΡΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΎΠ½ΠΊΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΏΡΠΎΡΠ΅ΡΡΠ° ΠΏΡΠΈ ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ ΡΠ΅ΡΠΈΠ΄ΠΈΠ²Π½ΠΎΠΉ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ, ΡΡΠΈΠ»Π΅Π½ΠΈΠΈ Π΅Π΅ Π²Π°ΡΠΊΡΠ»ΡΡΠΈΠ·Π°ΡΠΈΠΈ ΠΈ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠΈ ΡΠΊΠΎΡΠΎΡΡΠΈ ΠΊΡΠΎΠ²ΠΎΡΠΎΠΊΠ° Π½Π°Π±Π»ΡΠ΄Π°Π΅ΡΡΡ ΠΏΡΠΎΡΠΈΠ²ΠΎΠΏΠΎΠ»ΠΎΠΆΠ½ΠΎ Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½Π°Ρ Π΄ΠΈΠ½Π°ΠΌΠΈΠΊΠ° ΡΡΠΎΠ²Π½Ρ ΠΊΠ°ΡΠ±ΠΎΠ½ΠΈΠ»ΡΠ½ΡΡ
ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΡΡ
Π½Π΅ΠΉΡΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΈ ΠΎΡΠ½ΠΎΠ²Π½ΠΎΠ³ΠΎ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠ° (370 ΠΈ 530 Π½ΠΌ
ΠΠΠ£Π§ΠΠΠΠ ΠΠΠΠ ΠΠΠΠΠΠΠΠ‘Π’Π ΠΠΠ ΠΠΠΠ’ΠΠ ΠΠ£ΠΠ¬Π’Π£Π Π« HeLa ΠΠΠ’ΠΠΠΠ ΠΠΠ-ΠΠΠΠΠ’ ΠΠ Π ΠΠΠΠΠΠΠΠ ΠΠΠΠΠΠΠ ΠΠΠΠΠΠΠ‘Π’ΠΠΠ Π£ΠΠ¬Π’Π ΠΠΠΠ£ΠΠΠ Π 5-Π€Π’ΠΠ Π£Π ΠΠ¦ΠΠΠΠ
To understand the mechanism of the effect of 5 % 5-fluorouracil (5-FU) gel used simultaneously with ultrasound (US ) on a tumor in patients with cervical cancer, the level of DNA damage was studied in vitro. We used cervical adenocarcinoma HeLa CC L-2 cells cultured under standard conditions in RPMI-1640 medium with 10 % fetal bovine serum and 50 ΞΌg / ml gentamicin. Ultrasound exposure lasted 10 min. (frequency of 0.88 MHz and intensity of 0.2 W / cm2). The 5-FU dose was 0.7 ΠΌΠΊΠ, with the time of exposure of 24 hours. In 24 hours after starting exposure, the level of DNA damage to the cells of the culture was studied using the comet assay in the alkaline version and evaluated by the % DNA parameter in the comet tail (% TDNA). The statistical significance of the differences was evaluated using the Mann-Whitney U test and Fisherβs exact test. The combined exposure to ultrasound / 5-FU led to a 5.2-fold increase in % TDNA compared to the control culture, % TDNA was 1.9 times higher after exposure to 5-FU alone and 3.0 times higher after exposure to ultrasound alone. In 24 hours after the combined exposure, less than 50 % of the culture cells had a low level of DNA damage (<10 % TDNA), i.e. completed the repair process and could continue to proliferate, and more than 30 % of the culture cells still had a high level of damage (>30 % TDNA) and were probably in the process of apoptosis. Thus, the results of the study showed that the combined effect of ultrasound / 5-FU on the HeLa culture helped to overcome resistance to chemotherapy, having a synergistic effect.ΠΠ»Ρ ΠΏΠΎΠ½ΠΈΠΌΠ°Π½ΠΈΡ ΠΌΠ΅Ρ
Π°Π½ΠΈΠ·ΠΌΠ° ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π»ΠΎΠΊΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ 5 % Π³Π΅Π»Ρ 5-ΡΡΠΎΡΡΡΠ°ΡΠΈΠ»Π° (5-Π€Π£) Ρ ΠΎΠ΄Π½ΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΡΠΌ ΠΊΠΎΠ½ΡΠ°ΠΊΡΠ½ΡΠΌ ΡΠ»ΡΡΡΠ°Π·Π²ΡΠΊΠΎΠ²ΡΠΌ (Π£Π) Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ΠΌ Π½Π° ΠΎΠΏΡΡ
ΠΎΠ»Ρ Ρ Π±ΠΎΠ»ΡΠ½ΡΡ
ΡΠ°ΠΊΠΎΠΌ ΡΠ΅ΠΉΠΊΠΈ ΠΌΠ°ΡΠΊΠΈ Π² ΠΌΠΎΠ΄Π΅Π»ΡΠ½ΡΡ
ΡΡΠ»ΠΎΠ²ΠΈΡΡ
Π±ΡΠ» ΠΈΠ·ΡΡΠ΅Π½ ΡΡΠΎΠ²Π΅Π½Ρ ΠΏΠΎΠ²ΡΠ΅ΠΆΠ΄Π΅Π½Π½ΠΎΡΡΠΈ ΠΠΠ. ΠΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½Π° ΠΊΡΠ»ΡΡΡΡΠ° Π°Π΄Π΅Π½ΠΎΠΊΠ°ΡΡΠΈΠ½ΠΎΠΌΡ ΡΠ΅ΠΉΠΊΠΈ ΠΌΠ°ΡΠΊΠΈ HeLa CC L-2, ΠΊΡΠ»ΡΡΠΈΠ²ΠΈΡΠΎΠ²Π°Π½Π½Π°Ρ Π² ΡΡΠ°Π½Π΄Π°ΡΡΠ½ΡΡ
ΡΡΠ»ΠΎΠ²ΠΈΡΡ
Π² ΡΡΠ΅Π΄Π΅ RPMI-1640 Ρ Π΄ΠΎΠ±Π°Π²Π»Π΅Π½ΠΈΠ΅ΠΌ 10 % ΡΠ΅ΡΠ°Π»ΡΠ½ΠΎΠΉ ΡΡΠ²ΠΎΡΠΎΡΠΊΠΈ ΡΠ΅Π»Π΅Π½ΠΊΠ° ΠΈ 50 ΠΌΠΊΠ³/ΠΌΠ» Π³Π΅Π½ΡΠ°ΠΌΠΈΡΠΈΠ½Π°. ΠΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ ΡΠ»ΡΡΡΠ°Π·Π²ΡΠΊΠΎΠΌ ΠΎΡΡΡΠ΅ΡΡΠ²Π»ΡΠ»ΠΈ Ρ ΠΏΠΎΠΌΠΎΡΡΡ Π°ΠΏΠΏΠ°ΡΠ°ΡΠ° Π£ΠΠ’-1.03Π£ (Π ΠΎΡΡΠΈΡ) Π² ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ 10 ΠΌΠΈΠ½ Ρ ΡΠ°ΡΡΠΎΡΠΎΠΉ 0,88 ΠΠΡ ΠΈ ΠΈΠ½ΡΠ΅Π½ΡΠΈΠ²Π½ΠΎΡΡΡΡ 0,2 ΠΡ/ΡΠΌ2. ΠΠΎΠ·Π° 5-Π€Π£ ΡΠΎΡΡΠ°Π²ΠΈΠ»Π° 0,7 ΠΌΠΊΠ, Π²ΡΠ΅ΠΌΡ Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΡ 24 Ρ. Π§Π΅ΡΠ΅Π· 24 Ρ ΠΏΠΎΡΠ»Π΅ Π½Π°ΡΠ°Π»Π° Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΡ ΡΡΠΎΠ²Π΅Π½Ρ ΠΏΠΎΠ²ΡΠ΅ΠΆΠ΄Π΅Π½Π½ΠΎΡΡΠΈ ΠΠΠ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΊΡΠ»ΡΡΡΡΡ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π»ΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΠΠ-ΠΊΠΎΠΌΠ΅Ρ (Comet Assay) Π² ΡΠ΅Π»ΠΎΡΠ½ΠΎΠΌ Π²Π°ΡΠΈΠ°Π½ΡΠ΅ ΠΈ ΠΎΡΠ΅Π½ΠΈΠ²Π°Π»ΠΈ ΠΏΠΎ ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΡ % ΠΠΠ Π² Ρ
Π²ΠΎΡΡΠ΅ ΠΊΠΎΠΌΠ΅ΡΡ (% TDNA). ΠΠΎΠΌΠ±ΠΈΠ½ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠ΅ Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ Π£Π/5-Π€Π£ ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΠ»ΠΎ ΠΊ ΡΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠΈ Π·Π½Π°ΡΠΈΠΌΠΎΠΌΡ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΡ % TDNA β Π² 5,2 ΡΠ°Π·Π° ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΠΎΠΉ ΠΊΡΠ»ΡΡΡΡΠΎΠΉ, ΠΏΡΠΈ ΡΡΠΎΠΌ % TDNA Π±ΡΠ» Π² 1,9 ΡΠ°Π·Π° Π²ΡΡΠ΅ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Ρ ΠΏΠΎΡΠ»Π΅ Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΡ ΡΠΎΠ»ΡΠΊΠΎ 5-Π€Π£ ΠΈ Π² 3,0 ΡΠ°Π·Π° Π²ΡΡΠ΅ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Ρ ΠΏΠΎΡΠ»Π΅ Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΡ Π£Π. Π§Π΅ΡΠ΅Π· 24 Ρ ΠΏΠΎΡΠ»Π΅ ΠΊΠΎΠΌΠ±ΠΈΠ½ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΡ ΠΌΠ΅Π½Π΅Π΅ 50 % ΠΊΠ»Π΅ΡΠΎΠΊ ΠΊΡΠ»ΡΡΡΡΡ ΠΈΠΌΠ΅Π»ΠΈ Π½ΠΈΠ·ΠΊΠΈΠΉ ΡΡΠΎΠ²Π΅Π½Ρ ΠΏΠΎΠ²ΡΠ΅ΠΆΠ΄Π΅Π½Π½ΠΎΡΡΠΈ ΠΠΠ (<10 % TDNA), ΡΠΎ Π΅ΡΡΡ Π·Π°ΠΊΠΎΠ½ΡΠΈΠ»ΠΈ ΠΏΡΠΎΡΠ΅ΡΡ ΡΠ΅ΠΏΠ°ΡΠ°ΡΠΈΠΈ ΠΈ ΠΌΠΎΠ³Π»ΠΈ ΠΏΡΠΎΠ΄ΠΎΠ»ΠΆΠΈΡΡ ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΡ, Π° Π±ΠΎΠ»Π΅Π΅ 30 % ΠΊΠ»Π΅ΡΠΎΠΊ ΠΊΡΠ»ΡΡΡΡΡ Π²ΡΠ΅ Π΅ΡΠ΅ ΠΈΠΌΠ΅Π»ΠΈ Π²ΡΡΠΎΠΊΠΈΠΉ ΡΡΠΎΠ²Π΅Π½Ρ ΠΏΠΎΠ²ΡΠ΅ΠΆΠ΄Π΅Π½Π½ΠΎΡΡΠΈ (>30 % TDNA) ΠΈ, Π²Π΅ΡΠΎΡΡΠ½ΠΎ, Π½Π°Ρ
ΠΎΠ΄ΠΈΠ»ΠΈΡΡ Π² ΠΏΡΠΎΡΠ΅ΡΡΠ΅ Π°ΠΏΠΎΠΏΡΠΎΠ·Π°. Π’Π°ΠΊΠΈΠΌ ΠΎΠ±ΡΠ°Π·ΠΎΠΌ, ΠΊΠΎΠΌΠ±ΠΈΠ½ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠ΅ Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ Π£Π/5-Π€Π£ Π½Π° ΠΊΡΠ»ΡΡΡΡΡ HeLa ΡΠΏΠΎΡΠΎΠ±ΡΡΠ²ΠΎΠ²Π°Π»ΠΎ ΠΏΡΠ΅ΠΎΠ΄ΠΎΠ»Π΅Π½ΠΈΡ ΡΠ΅Π·ΠΈΡΡΠ΅Π½ΡΠ½ΠΎΡΡΠΈ ΠΊ Ρ
ΠΈΠΌΠΈΠΎΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΡ, ΠΎΠΊΠ°Π·ΡΠ²Π°Ρ ΡΠΈΠ½Π΅ΡΠ³ΠΈΡΠ΅ΡΠΊΠΈΠΉ ΡΡΡΠ΅ΠΊΡ
DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death
The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated to DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was followed-up. Kaplan-Meier analysis indicated a worse overall survival in the medium and high tertile of DNA damage (p < 0.001). The effect of DNA damage on survival was modelled according to Cox proportional hazard regression model. The adjusted hazard ratio (HR) was 1.42 (1.06-1.90) for overall mortality, and 1.94 (1.04-3.59) for diseases of the circulatory system in subjects with the highest tertile of DNA damage. The findings of this study provide epidemiological evidence encouraging the implementation of the comet assay in preventive strategies for non-communicable diseases
Π£ΡΠΎΠ²Π΅Π½Ρ Π½Π΅ΠΉΡΠΎΡΡΠΎΡΠΈΠ½ΠΎΠ² Π² Π³ΠΎΠ»ΠΎΠ²Π½ΠΎΠΌ ΠΌΠΎΠ·Π³Π΅ Ρ ΠΌΡΡΠ΅ΠΉ Ρ Π½ΠΎΠΊΠ°ΡΡΠΎΠΌ Π³Π΅Π½Π° ΡΡΠΎΠΊΠΈΠ½Π°Π·Ρ ΠΏΡΠΈ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΠΎΠΉ ΠΌΠ΅Π»Π°Π½ΠΎΠΌΠ΅ ΠΈ ΠΊΠΎΠΌΠΎΡΠ±ΠΈΠ΄Π½ΠΎΠΉ ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΠΈ
The objective was to evaluate the levels of neurotrophins in the brain of mice with urokinase (uPA) gene knockout, carriers of B16/F10 melanoma developing in presence of comorbid pathology β chronic neurogenic pain (CNP).Methods and materials. The study included female mice of two strains: Π‘57ΠL/6 (n=40) and C57BL/6-PlautmI.IBug-ThisPlau6FDhu/GFDhu (n=28). In the main groups, CNP was created by the bilateral sciatic nerve ligation, with Π16/F10 melanoma transplanted under the skin of the back 2 weeks after. The comparison groups included sham operated animals with melanoma transplantation, the control groups β sham operated animals and animals with CNP. Mice were decapitated on day 21 of the tumor growth, and the brain levels of brain neurotrophic factor (BDNF); nerve growth factor (NGF), neurotrophins 3 (NT3) and 4 (NT4) were studied by ELISA.Results. The brain of mice with uPA gene knockout demonstrated higher levels of NT3 (by 1.3 times (p=0.0146)), NT4 (by 2.6 times) and NGF-Ξ² (by 1.9 times (p=0.0021)) and lower BDNF (by 1.7 times (p=0.0203)), compared to mice without knockout. Cerebral reduction of NGF-Ξ² was a nonspecific brain response to CNP and neoplastic growth in female mice, enhanced in the combination of the pathological factors. Greater stimulation of subcutaneous melanoma growth in female mice with uPA knockout under the influence of CNP combined with a 2-fold decrease in levels of NT3 and BDNF in the brain, along with 2.2 times higher cerebral levels of NGF-Ξ², compared to female mice without knockout.Conclusions. In female mice with uPA gene knockout compared to mice without knockout, we revealed background differences and other dynamics of neurotrophin levels in the brain at melanoma growth both alone and in combination with comorbid pathology β CNP.Π¦Π΅Π»Ρ β ΠΈΠ·ΡΡΠΈΡΡ ΡΡΠΎΠ²Π΅Π½Ρ Π½Π΅ΠΉΡΠΎΡΡΠΎΡΠΈΠ½ΠΎΠ² Π² Π³ΠΎΠ»ΠΎΠ²Π½ΠΎΠΌ ΠΌΠΎΠ·Π³Π΅ ΠΌΡΡΠ΅ΠΉ Ρ Π½ΠΎΠΊΠ°ΡΡΠΎΠΌ Π³Π΅Π½Π° ΡΡΠΎΠΊΠΈΠ½Π°Π·Ρ (uPA), Π½ΠΎΡΠΈΡΠ΅Π»Π΅ΠΉ ΠΌΠ΅Π»Π°Π½ΠΎΠΌΡ B16/F10, ΡΠ°ΡΡΡΡΠ΅ΠΉ Π½Π° ΡΠΎΠ½Π΅ ΠΊΠΎΠΌΠΎΡΠ±ΠΈΠ΄Π½ΠΎΠΉ ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΠΈ β Ρ
ΡΠΎΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π½Π΅ΠΉΡΠΎΠ³Π΅Π½Π½ΠΎΠΉ Π±ΠΎΠ»ΠΈ (Π₯ΠΠ).ΠΠ΅ΡΠΎΠ΄Ρ ΠΈ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ. Π Π°Π±ΠΎΡΠ° Π²ΡΠΏΠΎΠ»Π½Π΅Π½Π° Π½Π° ΡΠ°ΠΌΠΊΠ°Ρ
ΠΌΡΡΠ΅ΠΉ Π»ΠΈΠ½ΠΈΠΉ Π‘57ΠL/6 (n=40) ΠΈ C57BL/6-PlautmI.IBugThisPlau6FDhu/GFDhu (n=28). Π ΠΎΡΠ½ΠΎΠ²Π½ΡΡ
Π³ΡΡΠΏΠΏΠ°Ρ
ΠΌΠΎΠ΄Π΅Π»ΠΈΡΠΎΠ²Π°Π»ΠΈ Π₯ΠΠ Π΄Π²ΡΡΡΠΎΡΠΎΠ½Π½Π΅ΠΉ ΠΏΠ΅ΡΠ΅Π²ΡΠ·ΠΊΠΎΠΉ ΡΠ΅Π΄Π°Π»ΠΈΡΠ½ΡΡ
Π½Π΅ΡΠ²ΠΎΠ² ΠΈ ΡΠ΅ΡΠ΅Π· 2 Π½Π΅Π΄Π΅Π»ΠΈ ΠΏΠΎΠ΄ ΠΊΠΎΠΆΡ ΡΠΏΠΈΠ½Ρ ΠΏΠ΅ΡΠ΅Π²ΠΈΠ²Π°Π»ΠΈ ΠΌΠ΅Π»Π°Π½ΠΎΠΌΡ Π16/F10. ΠΡΡΠΏΠΏΡ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ β Π»ΠΎΠΆΠ½ΠΎΠΎΠΏΠ΅ΡΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΠΆΠΈΠ²ΠΎΡΠ½ΡΠ΅ Ρ ΠΏΠ΅ΡΠ΅Π²ΠΈΠ²ΠΊΠΎΠΉ ΠΌΠ΅Π»Π°Π½ΠΎΠΌΡ. ΠΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΡΠ΅ Π³ΡΡΠΏΠΏΡ β Π»ΠΎΠΆΠ½ΠΎΠΎΠΏΠ΅ΡΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΠΆΠΈΠ²ΠΎΡΠ½ΡΠ΅ ΠΈ ΠΆΠΈΠ²ΠΎΡΠ½ΡΠ΅ Ρ Π₯ΠΠ. ΠΠ° 21-Π΅ ΡΡΡΠΊΠΈ ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΠΎΠ³ΠΎ ΡΠΎΡΡΠ° ΠΌΡΡΠ΅ΠΉ Π΄Π΅ΠΊΠ°ΠΏΠΈΡΠΈΡΠΎΠ²Π°Π»ΠΈ ΠΈ Π² Π³ΠΎΠ»ΠΎΠ²Π½ΠΎΠΌ ΠΌΠΎΠ·Π³Π΅ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΈΠΌΠΌΡΠ½ΠΎΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π° ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ Π½Π΅ΠΉΡΠΎΡΡΠΎΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠ°ΠΊΡΠΎΡΠ° ΠΌΠΎΠ·Π³Π° (BDNF); ΡΠ°ΠΊΡΠΎΡΠ° ΡΠΎΡΡΠ° Π½Π΅ΡΠ²ΠΎΠ² (NGF), Π½Π΅ΠΉΡΠΎΡΡΠΎΡΠΈΠ½ΠΎΠ²-3 (NT3) ΠΈ -4 (NT4).Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. Π£ ΠΌΡΡΠ΅ΠΉ Ρ Π½ΠΎΠΊΠ°ΡΡΠΎΠΌ ΠΏΠΎ uPA Π±ΡΠ»ΠΎ Π±ΠΎΠ»ΡΡΠ΅ NT3 (Π² 1,3 ΡΠ°Π·Π° (Ρ=0,0146)), NT4 (Π² 2,6 ΡΠ°Π·Π°) ΠΈ NGF-Ξ² (Π² 1,9 ΡΠ°Π·Π° (Ρ=0,0021)) ΠΈ ΠΌΠ΅Π½ΡΡΠ΅ BDNF (Π² 1,7 ΡΠ°Π·Π° (Ρ=0,0203)). ΠΠ΅ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΈΠΌ ΠΎΡΠ²Π΅ΡΠΎΠΌ Π³ΠΎΠ»ΠΎΠ²Π½ΠΎΠ³ΠΎ ΠΌΠΎΠ·Π³Π° ΡΠ°ΠΌΠΎΠΊ ΠΌΡΡΠ΅ΠΉ Π½Π° Π₯ΠΠ ΠΈ Π½Π΅ΠΎΠΏΠ»Π°ΡΡΠΈΡΠ΅ΡΠΊΠΈΠΉ ΡΠΎΡΡ ΡΠ²Π»ΡΠ»Π°ΡΡ ΡΠ΅ΡΠ΅Π±ΡΠ°Π»ΡΠ½Π°Ρ ΡΠ΅Π΄ΡΠΊΡΠΈΡ NGF-Ξ², Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΠΎΡΡΡ ΠΊΠΎΡΠΎΡΠΎΠΉ ΡΠ²Π΅Π»ΠΈΡΠΈΠ²Π°Π»Π°ΡΡ ΠΏΡΠΈ ΡΠΎΡΠ΅ΡΠ°Π½ΠΈΠΈ ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠ°ΠΊΡΠΎΡΠΎΠ². ΠΓ³Π»ΡΡΠ°Ρ ΡΡΠΈΠΌΡΠ»ΡΡΠΈΡ ΠΏΠΎΠ΄ΠΊΠΎΠΆΠ½ΠΎΠ³ΠΎ ΡΠΎΡΡΠ° ΠΌΠ΅Π»Π°Π½ΠΎΠΌΡ Ρ ΡΠ°ΠΌΠΎΠΊ ΠΌΡΡΠ΅ΠΉ Ρ Π½ΠΎΠΊΠ°ΡΡΠΎΠΌ uPA ΠΏΠΎΠ΄ Π²Π»ΠΈΡΠ½ΠΈΠ΅ΠΌ Π₯ΠΠ ΡΠΎΡΠ΅ΡΠ°Π»Π°ΡΡ Ρ Π΄Π²ΡΡ
ΠΊΡΠ°ΡΠ½ΡΠΌ ΡΠΌΠ΅Π½ΡΡΠ΅Π½ΠΈΠ΅ΠΌ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ NT3 ΠΈ BDNF Π² ΠΌΠΎΠ·Π³Π΅ Π½Π° ΡΠΎΠ½Π΅ Π² 2,2 ΡΠ°Π·Π° Π±Γ³Π»ΡΡΠ΅Π³ΠΎ, ΡΠ΅ΠΌ Ρ ΡΠ°ΠΌΠΎΠΊ Π±Π΅Π· Π½ΠΎΠΊΠ°ΡΡΠ°, ΡΠ΅ΡΠ΅Π±ΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΡΡΠΎΠ²Π½Ρ NGF-Ξ².ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. Π£ ΡΠ°ΠΌΠΎΠΊ ΠΌΡΡΠ΅ΠΉ Ρ Π½ΠΎΠΊΠ°ΡΡΠΎΠΌ Π³Π΅Π½Π° uPA, Π² ΠΎΡΠ»ΠΈΡΠΈΠ΅ ΠΎΡ ΠΌΡΡΠ΅ΠΉ Π±Π΅Π· Π½ΠΎΠΊΠ°ΡΡΠ°, Π²ΡΡΠ²Π»Π΅Π½Ρ ΡΠΎΠ½ΠΎΠ²ΡΠ΅ ΠΎΡΠ»ΠΈΡΠΈΡ ΠΈ ΠΈΠ½Π°Ρ Π΄ΠΈΠ½Π°ΠΌΠΈΠΊΠ° ΡΡΠΎΠ²Π½Π΅ΠΉ Π½Π΅ΠΉΡΠΎΡΡΠΎΡΠΈΠ½ΠΎΠ² Π² Π³ΠΎΠ»ΠΎΠ²Π½ΠΎΠΌ ΠΌΠΎΠ·Π³Π΅ ΠΏΡΠΈ ΡΠΎΡΡΠ΅ ΠΌΠ΅Π»Π°Π½ΠΎΠΌΡ Π² ΡΠ°ΠΌΠΎΡΡΠΎΡΡΠ΅Π»ΡΠ½ΠΎΠΌ Π²Π°ΡΠΈΠ°Π½ΡΠ΅ ΠΈ Π½Π° ΡΠΎΠ½Π΅ ΠΊΠΎΠΌΠΎΡΠ±ΠΈΠ΄Π½ΠΎΠΉ ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΠΈ β Π₯ΠΠ