Relationships among Lifestyle, Depression, Anxiety, and Reproductive Health in Female University Students

PURPOSE: To identify relationships among lifestyle, psychological distress, and reproductive health in female university students. METHODS: We used a descriptive cross-sectional design. A total of 275 subjects were enrolled. Subjects agreed to undergo a face-to-face interview, including administration of structured questionnaires to determine health promotion lifestyle profile II (HPLP-II), depression anxiety stress scale for depression and anxiety (DASS-D, DASS-A), and reproductive health (knowledge, attitude, behavior, menstruation irregularity, dysmenorrhea, and gynecologic symptoms). RESULTS: The mean age of subjects was 21.2 years. Those with low HPLP-II score showed significantly high menstruation irregularity. Those with high depression and anxiety had significantly high reproductive symptoms. In addition, high HPLP-II score was correlated with low depression, anxiety, and high reproductive health. CONCLUSION: The life style of female university students is associated with depression, anxiety, and reproductive health. To improve female university students' reproductive health, life style interventions including healthy nutritional habit, physical activity, and stress management are important

One-pot Enzymatic Synthesis of Deoxy-thymidine-diphosphate (TDP)-2-deoxy-∝-d-glucose Using Phosphomannomutase

Production of deoxy-thymidine-diphosphate (TDP)-sugars as substrates of glycosyltransferases, has been one of main hurdles for combinatorial antibiotic biosynthesis, which combines sugar moiety with aglycon of various antibiotics. Here, we report the one-pot enzymatic synthesis of TDP-2-deoxy-glucose employing high efficient TMP kinase (TMK; E.C. 2.7.2.12), acetate kinase (ACK; E.C. 2.7.1.21), and TDP-glucose synthase (TGS; E.C. 2.7.7.24) with phosphomannomutase (PMM; E.C. 5.4.2.8). In this study, replacing phosphoglucomutase (PGM; E.C. 5.4.2) by PMM from Escherichia coli gave four times higher specific activity on 2-deoxy-6-phosphate glucose, suggesting that the activity on 2-deoxy-glucose-6-phosphate was mainly affected by PMM activity, not PGM activity. Using an in vitro system starting from TMP and 2-deoxy-glucose-6-phosphate glucose, TDP-2-deoxy-glucose (63% yield) was successfully synthesized. Considering low productivity of NDP-sugars from cheap starting materials, this paper showed how production of NDP-sugars could be enhanced by controlling mutase activity

Molecular cloning and expression of a novel human cDNA related to the diazepam binding inhibitor

AbstractIn order to isolate the unidentified autoantigens in autoimmune diabetes, a human pancreatic islet cDNA library was constructed and screened with the sera from the diabetic patients. From the library screening, one clone (DRS-1) that strongly reacted with the sera was isolated. Subsequent sequence analysis revealed that the clone was a novel cDNA related to the diazepam binding inhibitor. DRS-1 was expressed in most tissues including liver, lung, tonsil, and thymus, in addition to pancreatic islets. DRS-1 was in vitro translated and the recombinant DRS-1 protein was expressed in Escherichia coli and purified. The size of the in vitro translated or bacterially expressed DRS-1 protein was in agreement with the conceptually translated polypeptide of DRS-1 cDNA. Further studies are required to test whether or not DRS-1 is a new autoantigen in autoimmune diabetes

CT Evidence for Subchondral Trabecular Injury of the Femoral Head in Transient Osteoporosis of the Hip: A Case Report

A 28-yr-old woman presented with both hip pain that started sequentially during the peripartum period. Diagnosis of transient osteoporosis of the hip (TOH) was made based on typical findings of plain radiographs and magnetic resonance images. The subchondral trabeculae of the femoral head were evaluated on serially taken coronal multiplanar reformation computerized tomogram images. At 4 weeks after pain onset, marked decrease in the sclerotic density with irregular discontinuation was observed in the primary compression trabeculae. At 12 weeks, a focal area of irregular thickening of trabeculae was observed. At 20 weeks, sclerotic density of trabeculae recovered markedly and the focal area of irregular trabecular thickening disappeared. At 1 yr, subchondral trabeculae recovered almost completely. The evidence of subchondral trabecular injury was observed in the femoral heads of TOH

Kaempferol inhibits IL‑1β‑induced proliferation of rheumatoid arthritis synovial fibroblasts and the production of COX‑2, PGE2 and MMPs

Inflammatory cytokines, matrix metalloproteinases (MMPs) and cyclooxygenase (COX)‑2 released from rheumatoid arthritis synovial fibroblasts (RASFs) are involved in the destruction of both articular bone and cartilage. Kaempferol has been reported to act as an antioxidant and anti‑inflammatory agent by inhibiting nitric oxide synthase and COX enzymes. The aim of the present study was to determine the effects of kaempferol on the interleukin‑1β (IL‑1β)‑induced proliferation of RASFs and the production of MMPs, COX and prostaglandin E2 (PGE2) by RASFs. The proliferation of the RASFs stimulated with IL‑1β and treated with/without kaempferol was evaluated by CCK‑8 assay. The expression of MMPs, TIMP metallopeptidase inhibitor‑1 (TIMP‑1), COXs, PGE2 and that of intracellular MAPK signaling molecules, including p‑ERK, p‑p38, p‑JNK and nuclear factor‑κB (NF‑κB) was examined by immunoblotting or semi‑quantitative reverse transcription‑polymerase chain reaction (RT‑PCR) and ELISA under the conditions described above. Kaempferol inhibited the proliferation of both unstimulated and IL‑1β‑stimulated RASFs, as well as the mRNA and protein expression of MMP‑1, MMP-3, COX‑2 and PGE2 induced by IL‑1β. Kaempferol also inhibited the phosphorylation of ERK‑1/2, p38 and JNK, as well as the activation of NF‑κB induced by IL‑1β. These results indicate that kaempferol inhibits synovial fibroblast proliferation, as well as the production of and MMPs, COX‑2 and PGE2, which is involved in articular inflammation and destruction in rheumatoid arthritis (RA). Our data suggest that kaempferol may be a novel therapeutic agent for the treatment of RA

Hydraulic and Structural Analysis of Complex Cross-Section Reinforced Concrete Pipes to Improve Sewage Flow in a Combined Sewer System

A complex cross-section reinforced concrete pipe that combines a sub-pipe for the flow of sewage in dry weather and a main pipe for the flow of rainwater was developed to reduce sedimentation of the combined sewer system in dry weather. The sub-pipe was designed, considering the flow velocity, constructability, and maintenance. By fitting the sewage data in the dry weather to the normal distribution, the ratio of the cross-sectional area of sewage flow to that of the pipe was determined to be approximately 0.418, which could cover 99.85% of the sewage volume of the target site. Based on this ratio, the diameter of the sub-pipe corresponding to the combined sewer system with a pipe diameter between 450 and 1300 mm was determined. The hydraulic performance analysis results showed that the flow velocity increased by 11 to 12% compared to the circular pipe based on the full sub-pipe and by more than 15% depending on the water level. The shear stress increased by more than 16.5%, and higher tractive force was observed. Structural safety was determined as the crack load and failure load far exceeded the minimum criteria, thereby verifying the feasibility and field applicability of the complex cross-section reinforced concrete pipe

Non-invasive flow mapping of parasagittal meningeal lymphatics using 2D interslice flow saturation MRI

The clearance pathways of brain waste products in humans are still under debate in part due to the lack of noninvasive imaging techniques for meningeal lymphatic vessels (mLVs). In this study, we propose a new noninvasive mLVs imaging technique based on an inter-slice blood perfusion MRI called alternate ascending/descending directional navigation (ALADDIN). ALADDIN with inversion recovery (IR) at single inversion time of 2300 ms (single-TI IR-ALADDIN) clearly demonstrated parasagittal mLVs around the human superior sagittal sinus (SSS) with better detectability and specificity than the previously suggested noninvasive imaging techniques. While in many studies it has been difficult to detect mLVs and confirm their signal source noninvasively, the detection of mLVs in this study was confirmed by their posterior to anterior flow direction and their velocities and morphological features, which were consistent with those from the literature. In addition, IR-ALADDIN was compared with contrast-enhanced black blood imaging to confirm the detection of mLVs and its similarity. For the quantification of flow velocity of mLVs, IR-ALADDIN was performed at three inversion times of 2000, 2300, and 2600 ms (three-TI IR-ALADDIN) for both a flow phantom and humans. For this preliminary result, the flow velocity of the dorsal mLVs in humans ranged between 2.2 and 2.7mm/s. Overall, (i) the single-TI IR-ALADDIN can be used as a novel non-invasive method to visualize mLVs in the whole brain with scan time of ~ 17min and (ii) the multi-TI IR-ALADDIN can be used as a way to quantify the flow velocity of mLVs with a scan time of ~ 10min (or shorter) in a limited coverage. Accordingly, the suggested approach can be applied to noninvasively studying meningeal lymphatic flows in general and also understanding the clearance pathways of waste production through mLVs in humans, which warrants further investigation

Analysis of the Flow Performance of the Complex Cross-Section Module to Reduce the Sedimentation in a Combined Sewer Pipe

The difference in the amount of stormwater and sewage in a combined sewer system is significantly large in areas where heavy rainfall is concentrated. This leads to a low water level and slow flow velocity inside the pipes, which causes sedimentation and odor on non-rainy days. A complex cross-section module increases the flow velocity by creating a small waterway inside the pipe for sewage to flow on non-rainy days. While considering Manning’s equation, we applied the principle where the flow velocity is proportional to two-thirds of the power of the hydraulic radius. The flow velocity of a circular pipe with a diameter of 450 mm and the corresponding complex cross-section module was analyzed by applying Manning’s equation and numerical modeling to show the effects of the complex cross-section module. The tractive force was compared based on a lab-scale experiment. When all conditions were identical except for the cross-sectional shape, the average flow velocity of the complex cross-section module was 14% higher while the size of the transported sand grains was up to 0.5 mm larger. This increase in flow velocity can be even higher if the roughness coefficient of aging pipes can be decreased