Peranan Dimetil Sulfoksida Sebagai Krioprotektan Dalam Mempertahankan Kualitas Spermatozoa Ikan Gurami, Osphronemus Goramy Lacepede, 1801, Dua Puluh Empat Jam Pascakriopreservasi

Penelitian dilakukan dengan tujuan mengetahui pengaruh berbegai konsentrasi DMSO (0%, 5%, 7%, 10%, 13%, 15%, dan 17%) sebagai krioprotektan terhadap kualitas spermatozoa ikan gurami, Osphronemus goramy Lacepede, 1801, dua puluh empat jam pascakriopreservasi. Penelitian dilakukan di Balai Besar Pengembangan Budidaya Air Tawar (BBPBAT), Sukabumi. Semen dikoleksi dengan cara pengurutan (stripping) dan dievaluasi secara makroskopis (warna, pH, dan volume) dan mikroskopis (persentase motilitas, viabilitas, abnormalitas, dan konsentrasi spermatozoa) baik sebelum maupun sesudah kriopreservasi. Semen diencerkan dengan pengencer yang mengandung ekstender 189M dan krioprotektan DMSO (0%, 5%, 7%, 10%, 13%, 15%, dan 17%), disimpan pada cryotube 2 ml dan dikriopreservasi selama 24 jam. Peningkatan konsentrasi DMSO memengaruhi kualitas spermatozoa ikan gurami pascakriopresevasi. Konsentrasi DMSO yang terbaik berdasarkan hasil pengamatan adalah DMSO konsentrasi 13% (P > 0,05) dengan persentase motilitas sebesar 68,58%; abnormalitas sebesar 29%; dan viabilitas sebesar 63,5%

Supplemental Enzyme in Artificial Diets for North African Catfish Larvae Clarias Gariepinus Burchell, 1822

Supplemental enzyme in artificial diets with difference doses for African catfish Clarias gariepinus (initial weight of 0.0048±0.0001 g and total length 0.7633±0.0392 cm) were examined for 12 days feeding trial. The aim of this study was to analyze the effectiveness supplemental enzymes in diets for growth performance of catfish larvae. Tubifex sp. (control) and a multi enzyme was included at the level of 0.00 (ME0), 1.00 (ME1) and 2.00 g kg-1 (ME2 diet as a test diets. Each of the four experimental diets was randomly assigned to four plicate groups. Larva was allocated into 150 L aquaria at a density of 1350 larvae per aquarium. Data was analyzed using SPSS 20, followed by Duncan's test. The result showed that the survival rate and condition factors were no statistical difference between experimental diets and control group. The highest final length, final weight, total food consumption, villi length and enzymes activity were recorded in the Control (P<0.05) and significantly different with other treatments. Feed efficiency's control significantly different with other. Harvest size distribution, in the small size group (1-2 cm) did not show significantly different, in the medium size group (2-3 cm) and large size group (3-5 cm) the Tubifex sp. treatment were significantly different from the other treatments (P<0.05) whereas between ME0, ME1 and ME2 treatments were not significantly different. Among the supplemental enzyme in test diets, ME2 can improve weight and total length 6.25% and 13.4%, respectively than ME0 treatment. The results suggested that enzyme supplementation can improve intestinal structure and growth performance of catfish larvae. The supplemental enzyme in diets with doses up to 2 g kg-1 can increase intestinal villi's length but have not been able to increase fish growth performance as use worm