Effect of Hedyotis diffusa Willd extract on gouty arthritis in rats

Purpose: To investigate the effect of Hedyotis diffusa Willd extract (HDWE) on gouty arthritis in rats.Method: Monosodium urate (MSU) crystal was injected into the ankle joint of rats to establish a rat model of gouty arthritis. HDWE (4.8, 9.6 and 19.2 g/kg) was administered to the rats treated with MSU crystals. The walking behavior of the rats was observed daily, and the gait score was calculated to evaluate the Oswestry disability index of rats. Levels of IL-1β and TNF-α in lavage fluid of articularcavities were measured using enzyme linked immunosorbent assay (ELISA) kits. The synovial tissues of joint of control, model and 19.2 g/kg HDWE group rats were obtained and NLRP3 inflammasome was analysed by Western blot.Results: The results showed that HDWE ameliorated the symptoms of gouty arthritis and gait score in rats significantly (p < 0.05). Further pharmacological experiments showed that all doses of HDWE decreased the levels of inflammatory cytokines IL-1β and TNF-α (p < 0.05), and inhibited NLRP3, caspase-1, ASC, IL-1β and IL-18 protein expressions of the lavage fluid of articular cavities in MSU crystal-treated rats (p < 0.01).Conclusion: The results indicate that HDWE exhibits a significant effect in ameliorating gouty arthritis via inhibition NLRP3  inflammasome, and thus is a potential new drug choice for the treatment of gouty arthritis. Keywords: Hedyotis diffusa, Caspase, Gouty arthritis, Inflammatory cytokines, NLRP3 inflammaso

Layout of Current US-China Online PD Programs for Chinese Language

Since the coronavirus outbreak, multiple academic institutions have held online seminars that have been beneficial to Chinese language teachers\u27 professional development (PD). However, it remains unknown whether the PD programs are appropriately designed regarding selecting topics and presenters. A total of 432 presentations of this study were retrieved from six Chinese and American organizations during the pandemic from May 1, 2020, to April 30, 2021. The content analysis approach was employed to analyze and categorize the framework of Technological Pedagogical Content and Teacher\u27s Soft-Skill Knowledge (TPACKS) into different themes and layouts of those presentations. The results indicate that Pedagogical Content Knowledge (PCK), Technological Pedagogical Knowledge (TPK), and Pedagogical Knowledge (PK) are more of a concern than other category components. The Technological Knowledge (TK), Content Knowledge (CK), Technological Content Knowledge (TCK), Technological Pedagogical, and Content Knowledge (TPACK) are a lower priority. The study also found that the number of independent presenters from universities is large, while that of K-12 school teacher presenters is low. However, there are many reports on cross-level cooperation among teachers in universities and K-12 schools. In addition, the teachers\u27 soft skills (S) categories have also received greater attention. Especially for increased awareness of non-traditional skills such as cross-cultural skills, meta-cognition and planning, and systemic thinking

Efficacy of chimeric antigen receptor T cell therapy and autologous stem cell transplant in relapsed or refractory diffuse large B-cell lymphoma: A systematic review

BackgroundWe aimed to compare the efficacy of chimeric antigen receptor T (CAR-T) cell therapy with that of autologous stem cell transplantation (auto-HSCT) in relapsed/refractory diffuse large B cell lymphoma (R/R DLBCL).Research design and methodsWe searched eligible publications up to January 31st, 2022, in PubMed, Cochrane Library, Springer, and Scopus. A total of 16 publications with 3484 patients were independently evaluated and analyzed using STATA SE software.ResultsPatients who underwent CAR-T cell therapy showed a better overall response rate (ORR) and partial response (PR) than those treated with auto-HSCT (CAR-T vs. auto-HSCT, ORR: 80% vs. 73%, HR:0.90,95%CI:0.76-1.07,P = 0.001; PR: 20% vs. 14%, HR:0.65,95%CI:0.62-0.68,P = 0.034). No significant difference was observed in 6-month overall survival (OS) (CAR-T vs. auto-HSCT, six-month OS: 81% vs. 84%, HR:1.23,95%CI:0.63-2.38, P = 0.299), while auto-HSCT showed a favorable 1 and 2-year OS (CAR-T vs. auto-HSCT, one-year OS: 64% vs. 73%, HR:2.42,95%CI:2.27-2.79, P < 0.001; two-year OS: 54% vs. 68%, HR:1.81,95%CI:1.78-1.97, P < 0.001). Auto-HSCT also had advantages in progression-free survival (PFS) (CAR-T vs. auto-HSCT, six-month PFS: 53% vs. 76%, HR:2.81,95%CI:2.53-3.11,P < 0.001; one-year PFS: 46% vs. 61%, HR:1.84,95%CI:1.72-1.97,P < 0.001; two-year PFS: 42% vs. 54%, HR:1.62,95%CI:1.53-1.71, P < 0.001). Subgroup analysis by age, prior lines of therapy, and ECOG scores was performed to compare the efficacy of both treatment modalities.ConclusionAlthough CAR-T cell therapy showed a beneficial ORR, auto-HSCT exhibited a better long-term treatment superiority in R/R DLBCL patients. Survival outcomes were consistent across different subgroups

Paleoclimate Records of the Middle Okinawa Trough Since the Middle Holocene: Modulation of the Low-Latitude Climate

The ubiquity of glycerol dibiphytanyl glycerol tetraethers (GDGTs) and their temperature sensitivity make them one of the most effective tools for paleoclimate reconstruction. High- and low-latitude climates influence the Okinawa Trough (OT). It receives diverse inputs from the East China Sea, the western Pacific, and the Kuroshio Current, providing good conditions for paleoclimate studies. Here, isoprenoid GDGTs (isoGDGTs), branched GDGTs, and hydroxylated GDGTs (OH-GDGTs) were studied to reconstruct the sea surface temperature (SST) of the central OT for the past 8.2 kyr using the tetraether index of 86 carbon atoms at low latitudes (TEX86H) and the ring index of OH-GDGTs (RI-OH). The GDGT-0/crenarchaeol ratios ranged from 0.39 to 0.98. The branched and isoprenoid tetraether index and the methane index values were lower than 0.1 and 0.5, respectively, indicating that the isoGDGTs were mainly derived from marine Thaumarchaeota and that TEX86H could be used to reconstruct the paleotemperatures. The TEX86H SSTs ranged from 21.6 to 27.2 degrees C during 8.2 kyr. The overall range of TEX86H SSTs is close to the U-37(K') SST of the middle OT and reflects the mean annual SST. In contrast, RI-OH temperatures varied from 17.4 to 26.0 degrees C, showing a lower trend than TEX86H SSTs. The core top RI-OH temperature is 24.1 degrees C, in line with the mean annual seawater temperature at 40 m (24.2 degrees C) in the study area, which likely reflects the subsurface temperature in this case. The small overall warming trend of TEX86H SSTs agrees with the increasing intensity of the Kuroshio Current during the last 8.2 kyr, indicating that the SST evolution is governed by the Kuroshio Current that transports heat from the western tropical Pacific. The decreasing temperature differences between TEX86H and RI-OH and between U-37(K') and RI-OH showed increased mixing of the upper water column, which was in good accordance with the increasing low-latitude winter insolation decoupling from the East Asian summer monsoon. The cold event that occurred at 7.4-6.6 kyr was magnified (similar to 5 degrees C) at the TEX86H and RI-OH temperatures and possibly caused by tephra's significant input (similar to 7.3 kyr)

Bilayer- and Bulk-Heterojunction Solar Cells Using Liquid Crystalline Porphyrins as Donors by Solution Processing

Bilayer- and bulk-heterojunction solar cells based on liquid crystalline porphyrins (donors) were fabricated by solution processing. These porphyrins are (i) highly absorptive over the wavelengths of the solar spectrum, (ii) having energy levels matched well with the electron acceptors and anode materials to facilitate charge separation and transfer, and (iii) of a unique homeotropically aligned architecture for efficient charge transport and light harvesting. Thermal annealing of these solar cells induced alignments of porphyrins in the photoactive layers, leading to a factor of 4-5 higher power conversion efficiencies and short circuit current densities than their counterpart devices without postannealing. (c) 2007 American Institute of Physics.</p

Protective effect of Guanxin Danshen formula on myocardial ischemiareperfusion injury in rats

ABSTRACT Purpose: Myocardial ischemia/reperfusion injury (MIRI) leads to myocardial tissue necrosis, which will increase the size of myocardial infarction. The study examined the protective effect and mechanism of the Guanxin Danshen formula (GXDSF) on MIRI in rats. Methods: MIRI model was performed in rats; rat H9C2 cardiomyocytes were hypoxia-reoxygenated to establish a cell injury model. Results: The GXDSF significantly reduced myocardial ischemia area, reduced myocardial structural injury, decreased the levels of interleukin (IL-1β, IL-6) in serum, decreased the activity of myocardial enzymes, increased the activity of superoxide dismutase (SOD), and reduced glutathione in rats with MIRI. The GXDSF can reduce the expression of nucleotide- binding oligomerization domain, leucine-rich repeat and pyrin domain containing nod-like receptor family protein 3 (NLRP3), IL-1β, caspase-1, and gasdermin D (GSDMD) in myocardial tissue cells. Salvianolic acid B and notoginsenoside R1 protected H9C2 cardiomyocytes from hypoxia and reoxygenation injury and reduced the levels of tumor necrosis factor α (TNF-α) and IL-6 in the cell supernatant, decreasing the NLRP3, IL-18, IL-1β, caspase-1, and GSDMD expression in H9C2 cardiomyocytes. GXDSF can reduce the myocardial infarction area and alleviate the damage to myocardial structure in rats with MIRI, which may be related to the regulation of the NLRP3. Conclusions: GXDSF reduces MIRI in rat myocardial infarction injury, improves structural damage in myocardial ischemia injury, and reduces myocardial tissue inflammation and oxidative stress by lowering inflammatory factors and controlling focal cell death signaling pathways

Mucoepidermoid carcinoma arising in Warthins tumor of the upper lip: a case report and review

Objective To provide a reference for the diagnosis and treatment of mucoepidermoid carcinoma arising in Warthins tumor of the lip by investigating the diagnosis, treatment and prognosis of the disease. Methods A case of mucoepidermoid carcinoma arising in Warthins tumor of lip was reported, including the clinical manifestation, treat⁃ ment, pathological characteristics and prognosis. The related literature was also reviewed and analyzed. Results A painless mass on the left lip lasting more than one month was found. Resection of the left lip was performed. Pathologi⁃ cal examination showed that the tumor was a hybridoma composed of mucoepidermoid carcinoma and Warthins tumor. There was no recurrence or distant metastasis after 34 months. To date, this type of disease has been rarely reported. Af⁃ ter thorough resection, the prognosis and survival rate are promising in most cases, with no recurrence or metastasis. Conclusion Mucoepidermoid carcinoma in Warthins tumor of the lip is rare. Clinical manifestations, imaging features and histological examination are useful when diagnosing the disease. Thorough resection will reduce the risk of disease recurrence

Pulmonary delivery of mucus-traversing PF127-modified silk fibroin nanoparticles loading with quercetin for lung cancer therapy

The mucosal barrier remains a major barrier in the pulmonary drug delivery system, as mucociliary clearance in the airway accelerates the removal of inhaled nanoparticles (NPs). Herein, we designed and developed the inhalable Pluronic F127-modified silk fibroin NPs loading with quercetin (marked as QR-SF (PF127) NPs), aiming to solve the airway mucus barrier and improve the cancer therapeutic effect of QR. The PF127 coating on the SF NPs could attenuate the interaction between NPs and mucin proteins, thus facilitating the diffusion of SF(PF127) NPs in the mucus layer. The QR-SF (PF127) NPs had particle sizes of approximately 200 nm with negatively charged surfaces and showed constant drug release properties. Fluorescence recovery after photobleaching (FRAP) assay and transepithelial transport test showed that QR-SF (PF127) NPs exhibited superior mucus-penetrating ability in artificial mucus and monolayer Calu-3 cell model. Notably, a large amount of QR-SF (PF127) NPs distributed uniformly in the mice airway section, indicating the good retention of NPs in the respiratory tract. The mice melanoma lung metastasis model was established, and the therapeutic effect of QR-SF (PF127) NPs was significantly improved in vivo. PF127-modified SF NPs may be a promising strategy to attenuate the interaction with mucin proteins and enhance mucus penetration efficiency in the pulmonary drug delivery system