A Human Torque Teno Virus Encodes a MicroRNA That Inhibits Interferon Signaling

Rodney P. Kincaid, James M. Burke, Jennifer C. Cox, Christopher S. Sullivan, The University of Texas at Austin, Molecular Genetics and Microbiology, Austin, Texas, United States of AmericaEthel-Michele de Villiers, Division for the Characterization of Tumorviruses, Deutsches Krebsforschungszentrum, Heidelberg, GermanyTorque teno viruses (TTVs) are a group of viruses with small, circular DNA genomes. Members of this family are thought to ubiquitously infect humans, although causal disease associations are currently lacking. At present, there is no understanding of how infection with this diverse group of viruses is so prevalent. Using a combined computational and synthetic approach, we predict and identify miRNA-coding regions in diverse human TTVs and provide evidence for TTV miRNA production in vivo. The TTV miRNAs are transcribed by RNA polymerase II, processed by Drosha and Dicer, and are active in RISC. A TTV mutant defective for miRNA production replicates as well as wild type virus genome; demonstrating that the TTV miRNA is dispensable for genome replication in a cell culture model. We demonstrate that a recombinant TTV genome is capable of expressing an exogenous miRNA, indicating the potential utility of TTV as a small RNA vector. Gene expression profiling of host cells identifies N-myc (and STAT) interactor (NMI) as a target of a TTV miRNA. NMI transcripts are directly regulated through a binding site in the 3′UTR. SiRNA knockdown of NMI contributes to a decreased response to interferon signaling. Consistent with this, we show that a TTV miRNA mediates a decreased response to IFN and increased cellular proliferation in the presence of IFN. Thus, we add Annelloviridae to the growing list of virus families that encode miRNAs, and suggest that miRNA-mediated immune evasion can contribute to the pervasiveness associated with some of these viruses.This work was supported by grants RO1AI077746 from the National Institutes of Health, RP110098 from the Cancer Prevention and Research Institute of Texas, a Burroughs Wellcome Investigators in Pathogenesis Award to CSS, a UT Austin Powers Graduate Fellowship to RPK, a UT Austin Institute for Cellular and Molecular Biology fellowship, and the DKFZ for EMdV. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Molecular BiosciencesMicrobiologyEmail: [email protected]

Terror as Repression Inside the Favelas of Rio de Janeiro

This article was published in the Fall 2009 issue of the Journal of Undergraduate Researc

Evolving Strategies, Opportunistic Implementation: HIV Risk Reduction in Tanzania in the Context of an Incentive-Based HIV Prevention Intervention.

\ud Behavior change communication (BCC) interventions, while still a necessary component of HIV prevention, have not on their own been shown to be sufficient to stem the tide of the epidemic. The shortcomings of BCC interventions are partly due to barriers arising from structural or economic constraints. Arguments are being made for combination prevention packages that include behavior change, biomedical, and structural interventions to address the complex set of risk factors that may lead to HIV infection. In 2009/2010 we conducted 216 in-depth interviews with a subset of study participants enrolled in the RESPECT study - an HIV prevention trial in Tanzania that used cash awards to incentivize safer sexual behaviors. We analyzed community diaries to understand how the study was perceived in the community. We drew on these data to enhance our understanding of how the intervention influenced strategies for risk reduction. We found that certain situations provide increased leverage for sexual negotiation, and these situations facilitated opportunistic implementation of risk reduction strategies. Opportunities enabled by the RESPECT intervention included leveraging conditional cash awards, but participants also emphasized the importance of exploiting new health status knowledge from regular STI testing. Risk reduction strategies included condom use within partnerships and/or with other partners, and an unexpected emphasis on temporary abstinence. Our results highlight the importance of increasing opportunities for implementing risk reduction strategies. We found that an incentive-based intervention could be effective in part by creating such opportunities, particularly among groups such as women with limited sexual agency. The results provide new evidence that expanding regular testing of STIs is another important mechanism for providing opportunities for negotiating behavior change, beyond the direct benefits of testing. Exploiting the latent demand for STI testing should receive renewed attention as part of innovative new combination interventions for HIV prevention.\u

At The Coffee Cooler\u27s Tea

https://digitalcommons.library.umaine.edu/mmb-vp/4852/thumbnail.jp

Dense embeddings of surface groups

We discuss dense embeddings of surface groups and fully residually free groups in topological groups. We show that a compact topological group contains a nonabelian dense free group of finite rank if and only if it contains a dense surface group. Also, we obtain a characterization of those Lie groups which admit a dense faithfully embedded surface group. Similarly, we show that any connected semisimple Lie group contains a dense copy of any fully residually free group.Comment: This is the version published by Geometry & Topology on 4 October 200

Phase shift in experimental trajectory scaling functions

For one dimensional maps the trajectory scaling functions is invariant under coordinate transformations and can be used to compute any ergodic average. It is the most stringent test between theory and experiment, but so far it has proven difficult to extract from experimental data. It is shown that the main difficulty is a dephasing of the experimental orbit which can be corrected by reconstructing the dynamics from several time series. From the reconstructed dynamics the scaling function can be accurately extracted.Comment: CYCLER Paper 93mar008. LaTeX, LAUR-92-3053. Replaced with a version with all figure

I Can\u27t See The Good In Good-bye

https://digitalcommons.library.umaine.edu/mmb-vp/5947/thumbnail.jp

On smoothable surgery for 4-manifolds

Under certain homological hypotheses on a compact 4-manifold, we prove exactness of the topological surgery sequence at the stably smoothable normal invariants. The main examples are the class of finite connected sums of 4-manifolds with certain product geometries. Most of these compact manifolds have non-vanishing second mod 2 homology and have fundamental groups of exponential growth, which are not known to be tractable by Freedman-Quinn topological surgery. Necessarily, the *-construction of certain non-smoothable homotopy equivalences requires surgery on topologically embedded 2-spheres and is not attacked here by transversality and cobordism.Comment: 18 pages, separated into two journal submission