MiR-384 is associated with renal damage in lupus nephritis via regulation of TET3 expression

Purpose: To investigate the correlations between miR-384 expression and renal damage in lupus nephritis (LN).Methods: Lupus nephritis and normal tissues were collected during surgery. The relative miR-384 expression was evaluated by extracting RNA and performing quantitative real time PCR (qRT-PCR) assays. Expression of ten-eleven translocation (TET3) mRNA and protein were measured by qRT-PCR and western blotting, respectively. The 24-h urine protein, serum complement C3, and serum creatinine were determined using commercial enzyme-linked immunosorbent assay (ELISA) kits. TargetScan and luciferase assays were used to validate the binding site for miR-384 and its target mRNA. Relationships among miR-384, TET3, and renal damage were analyzed by Spearman rank-order correlation coefficients.Results: MiR-384 expression increased in LN tissues and was positively correlated with the activity index (AI) and chronicity index of LN, whereas miR-384 expression and serum complement C3 were negatively correlated. Positive correlations were observed between miR-384 expression and 24-h urine protein, serum creatinine, and systemic lupus erythematosus disease AI. TargetScan and luciferaseassays indicated that the TET3 3′-UTR was the direct target of miR-384. MiR-384 upregulation inhibited TET3 mRNA and protein  expression, and was negatively associated with renal damage in LN.Conclusion: MiR-384 upregulation contributes to renal damage in LN by targeting the 3′-UTR of TET3 mRNA, suggesting that miR-384 is a potential biomarker and therapeutic target in LN. Keywords: MiR-384, Renal damage, Lupus nephritis, Ten-eleven translocation, TET

Future potential evapotranspiration changes and contribution analysis in Zhejiang Province, East China

PublishedJournal ArticleThis is the final version of the article. Available from Wiley via the DOI in this record.Potential evapotranspiration is an important component of hydrological modeling. In this study, the objective is to project potential evapotranspiration in the future period 2011-2040 and understand their changes in Zhejiang Province, East China. The sensitivity of potential evapotranspiration to five climatic variables (solar radiation, daily minimum and maximum air temperature, relative humidity, and wind speed) is analyzed based on observation data from 1955-2008 using a global sensitivity analysis method, Sobol's method. The changes in potential evapotranspiration during the future period are generated using one regional climate model, Providing Regional Climates for Impacts Studies, with two global climate models, ECHAM5 and Hadley Centre Coupled Model version 3, and their causes are analyzed based on sensitivity analysis results. Global sensitivity analysis results reveal substantial spatial-temporal variations in the sensitivity of potential evapotranspiration to climatic variables and unignorable interactions among climatic variables. Rather similar spatial change patterns of annual mean potential evapotranspiration (PET) are generated for both general circulation models; however, seasonal or monthly changes are very different due to different spatial-temporal changes in climatic variables. Different contributory sources to potential evapotranspiration changes are identified at different months and stations; the PET changes in 2011-2040 are mainly due to three climatic variables including solar radiation, relative humidity, and daily minimum temperature. © 2014. American Geophysical Union. All Rights Reserved

A clinical study of mycophenolate mofetil combined with low-dose steroids in the management of pediatric systemic lupus erythematosus

Purpose: To investigate the efficacy and safety of mycophenolate mofetil (MMF) combined with lowdose steroids in the treatment of pediatric systemic lupus erythematosus (pSLE).Methods: A total of 76 children were diagnosed and admitted with SLE, lupus nephritis (LN) and type IV diffuse proliferative glomerulonephritis at Hainan Women and Children’s Medical Center, Haikou, China from March 2017 to December 2018, had their clinical data analyzed retrospectively. Among them, 38 children received methylprednisolone pulse combined with MMF and intermittent oral low-dose glucocorticoids (GC), labelled MMF group, while the remaining 38 children, which served as control group, were treated with oral GC and transitional reduction. Pertinent biochemical parameters were evaluatedResults: Compared with control group, MMF group showed a notably lower level of erythrocyte sedimentation rate (ESR), a higher level of complement C3, lower level of serum creatinine (Scr) and 24-h urine protein level 6 months after treatment (p < 0.05). Albumin level was higher in MMF group at 6 months and 12 months after treatment than in the control group. Compared to control group, the SLEDAI score in MMF group was significantly lower at 6 months and 12 months after treatment (p < 0.05). Body mass index, triglyceride, fasting blood glucose and intraocular pressure levels in the MMF group were significantly lower than those in the control group (p < 0.05). Post-treatment, peripheral blood CD3+ and CD4+ T lymphocytes, CD4+/CD8+ ratio and NK cell levels in the two groups were significantly increased, while CD8+ T lymphocyte level declined.Conclusion: MMF combined with low-dose methylprednisolone controls symptoms early and mitigates renal injury in the treatment of pSLE. It is also safe, and effectively regulates the patient's cellular immune function

Preparation and biological activity of the monoclonal antibody against the second extracellular loop of the angiotensin II type 1 receptor

The current study was to prepare a mouse-derived antibody against the angiotensin II type 1 receptor (AT1-mAb) based on monoclonal antibody technology, to provide a foundation for research on AT1-AA-positive diseases. Balb/C mice were actively immunized with the second extracellular loop of the angiotensin II type 1 receptor (AT1R-ECII). Then, mouse spleen lymphocytes were fused with myeloma cells and monoclonal hybridomas that secreted AT1-mAb were generated and cultured, after which those in logarithmic-phase were injected into the abdominal cavity of mice to retrieve the ascites. Highly purified AT1-mAb was isolated from mouse ascites after injection with 1 × 107 hybridomas. A greater amount of AT1-mAb was purified from mouse ascites compared to the cell supernatant of hybridomas. AT1-mAb purified from mouse ascites constricted the thoracic aorta of mice and increased the beat frequency of neonatal rat myocardial cells via the AT1R, identical to the effects of AT1-AA extracted from patients’ sera. Murine blood pressure increased after intravenous injection of AT1-mAb via the tail vein. High purity and good biological activity of AT1-mAb can be obtained from mouse ascites after intraperitoneal injection of monoclonal hybridomas that secrete AT1-mAb. These data provide a simple tool for studying AT1-AA-positive diseases

Coronal mini-jets in an activated solar tornado-like prominence

High-resolution observations from the Interface Region Imaging Spectrometer reveal the existence of a particular type of small solar jet, which arose singly or in clusters from a tornado-like prominence suspended in the corona. In this study, we perform a detailed statistical analysis of 43 selected mini-jets in the tornado event. Our results show that the mini-jets typically have (1) a projected length of 1.0–6.0 Mm, (2) a width of 0.2–1.0 Mm, (3) a lifetime of 10–50 s, (4) a velocity of 100–350 km s−1, and (5) an acceleration of 3–20 km s−2. Based on spectral diagnostics and EM-Loci analysis, these jets seem to be multithermal small-scale plasma ejections with an estimated average electron density of ~2.4  ×  1010 cm−3 and an approximate mean temperature of ~2.6  ×  105 K. Their mean kinetic energy density, thermal energy density, and dissipated magnetic field strength are roughly estimated to be ~9 erg cm−3, 3 erg cm−3, and 16 G, respectively. The accelerations of the mini-jets, the UV and EUV brightenings at the footpoints of some mini-jets, and the activation of the host prominence suggest that the tornado mini-jets are probably created by fine-scale external or internal magnetic reconnections (a) between the prominence field and the enveloping or background field or (b) between twisted or braided flux tubes within the prominence. The observations provide insight into the geometry of such reconnection events in the corona and have implications for the structure of the prominence magnetic field and the instability that is responsible for the eruption of prominences and coronal mass ejections.Publisher PDFPeer reviewe

Adiponectin improves coronary no-reflow injury by protecting the endothelium in rats with type 2 diabetes mellitus.

To determine the effect of adiponectin (APN) on the coronary no-reflow (NR) injury in rats with Type 2 diabetes mellitus (T2DM), 80 male Sprague-Dawley rats were fed with a high-sugar-high-fat diet to build a T2DM model. Rats received vehicle or APN in the last week and then were subjected to myocardial ischemia reperfusion (MI/R) injury. Endothelium-dependent vasorelaxation of the thoracic aorta was significantly decreased and serum levels of endothelin-1 (ET-1), intercellular cell adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) were noticably increased in T2DM rats compared with rats without T2DM. Serum APN was positively correlated with the endothelium-dependent vasorelaxation, but negatively correlated with the serum level of ET-1. Treatment with APN improved T2DM-induced endothelium-dependent vasorelaxation, recovered cardiac function, and decreased both NR size and the levels of ET-1, ICAM-1 and VCAM-1. Hypoadiponectinemia was associated with the aggravation of coronary NR in T2DM rats. APN could alleviate coronary NR injury in T2DM rats by protecting the endothelium and improving microcirculation