Precise method to control elastic waves by conformal mapping

AbstractThe transformation method to control waves has received widespread attention in electromagnetism and acoustics. However, this machinery is not directly applicable to the control of elastic waves, because it has been shown that the Navier's equation does not usually retain its form under coordinate transformation. In this letter, we prove the form invariance of the Navier's equation under the conformal mapping based on the Helmholtz decomposition method. The needed material parameters are provided to manipulate elastic waves. The validity of this approach is confirmed by an active stealth device which can disguise the signal source by changing its position. Experimental verifications and potential applications may be expected in nondestructive testing, structural seismic design and other fields

Population genetic structure and demographic history of small yellow croaker, Larimichthys polyactis (Bleeker, 1877), from coastal waters of China

Small yellow croaker, Larimichthys polyactis (Bleeker, 1877), a commercially important benthopelagic fish, is widely distributed in the Bohai, Yellow and East China Seas. To evaluate the population genetic structure and demographic history of L. polyactis, we sequenced the complete mitochondrial deoxyribonucleic acid (mtDNA) control region (798 to 801 bp) in 127 individuals sampled from seven localities throughout its distribution region in China. A total of 136 polymorphic sites were detected, which defined 125 haplotypes. High haplotype diversity (1.000 ± 0.013 to 1.000 ± 0.034) and moderate nucleotide diversity (0.0112 ± 0.0061 to 0.0141 ± 0.0075) were detected in the species. The neighbor-joining tree of haplotypes was assigned into two closely related clades, but did not appear to have any geographic genealogic structure. Hierarchical molecular variance analysis (AMOVA), pair wise FST comparisons and the nearest-neighbor statistic (Snn) showed no significant genetic differences among populations in the Bohai, Yellow and East China Seas. The demographic history of L. polyactis was examined by using neutrality tests and mismatch distribution analysis, which revealed that the species had undergone a Pleistocene population expansion. The results based on the complete mtDNA control region sequences analysis indicate that within its distribution range, L. polyactis constituted a panmictic mtDNA gene pool. Factors such as dispersal capacity, ocean currents and insufficient evolution time could be responsible for the lack of population genetic differentiation in L. polyactis.Keywords: Larimichthys polyactis, mitochondrial control region, population genetic structure, demographi

Molecular cytogenetic analyses of Epinephelus bruneus and Epinephelus moara (Perciformes, Epinephelidae)

Genus Epinephelus (Perciformes, Epinephelidae), commonly known as groupers, are usually difficult in species identification for the lack and/or change of morphological specialization. In this study, molecular cytogenetic analyses were firstly performed to identify the closely related species Epinephelus bruneus and E. moara in this genus. The species-specific differences of both fish species showed in karyotype, chromosomal distribution of nucleolar organizer regions (NORs) and localization of 18S rDNA. The heterochromatin (interstitial C-bands) and distribution pattern of telomere (TTAGGG)n in E. bruneus revealed the chromosomal rearrangements and different karyotypic evolutionary characteristics compared to those in E. moara. The cytogenetic data suggested that the lineages of E. bruneus and E. moara were recently derived within the genus Epinephelus, and E. moara exhibited more plesiomorphic features than E. bruneus. All results confirmed that E. moara, which has long been considered a synonym of E. bruneus, is a distinct species in the family Epinephelidae. In addition, molecular cytogenetic analyses are useful in species differentiation and phylogenetic reconstruction in groupers

Dissipative elastic metamaterial with a lowfrequency passband

We design and experimentally demonstrate a dissipative elastic metamaterial structure that functions as a bandpass filter with a low-frequency passband. The mechanism of dissipation in this structure is well described by a mass-spring-damper model that reveals that the imaginary part of the wavenumber is non-zero, even in the passband of dissipative metamaterials. This indicates that transmittance in this range can be low. A prototype for this viscoelastic metamaterial model is fabricated by 3D printing techniques using soft and hard acrylics as constituent materials. The transmittance of the printed metamaterial is measured and shows good agreement with theoretical predictions, demonstrating its potential in the design of compact waveguides, filters and other advanced devices for controlling mechanical waves

Dissipative elastic metamaterial with a lowfrequency passband

We design and experimentally demonstrate a dissipative elastic metamaterial structure that functions as a bandpass filter with a low-frequency passband. The mechanism of dissipation in this structure is well described by a mass-spring-damper model that reveals that the imaginary part of the wavenumber is non-zero, even in the passband of dissipative metamaterials. This indicates that transmittance in this range can be low. A prototype for this viscoelastic metamaterial model is fabricated by 3D printing techniques using soft and hard acrylics as constituent materials. The transmittance of the printed metamaterial is measured and shows good agreement with theoretical predictions, demonstrating its potential in the design of compact waveguides, filters and other advanced devices for controlling mechanical waves

Integration of Transcriptomic and Proteomic Approaches Reveals the Temperature-Dependent Virulence of Pseudomonas plecoglossicida

Pseudomonas plecoglossicida is a facultative pathogen that is associated with diseases of multiple fish, mainly at 15–20°C. Although fish disease caused by P. plecoglossicida has led to significant economic losses, the mechanisms of the temperature-dependent virulence are unclear. Here, we identify potential pathogenicity mechanisms and demonstrate the direct regulation of several virulence factors by temperature with transcriptomic and proteomic analyses, quantitative real-time PCR (qRT-PCR), RNAi, pyoverdine (PVD) quantification, the chrome azurol S (CAS) assay, growth curve measurements, a biofilm assay, and artificial infection. The principal component analysis, the heat map generation and hierarchical clustering, together with the functional annotations of the differentially expressed genes (DEGs) demonstrated that, under different growth temperatures, the animation and focus of P. plecoglossicida are quite different, which may be the key to pathogenicity. Genes involved in PVD synthesis and in the type VI secretion system (T6SS) are specifically upregulated at the virulent temperature of 18°C. Silencing of the PVD-synthesis-related genes reduces the iron acquisition, growth, biofilm formation, distribution in host organs and virulence of the bacteria. Silencing of the T6SS genes also leads to the reduction of biofilm formation, distribution in host organs and virulence. These findings reveal that temperature regulates multiple virulence mechanisms in P. plecoglossicida, especially through iron acquisition and T6SS secretion. Meanwhile, integration of transcriptomic and proteomic data provide us with a new perspective into the pathogenesis of P. plecoglossicida, which would not have been easy to catch at either the protein or mRNA differential analyses alone, thus illustrating the power of multi-omics analyses in microbiology

Cross talk between heat shock protein 10 and a heat shock factor identified from Marsupenaeus japonicus

Abstract(#br)Heat shock factors (HSFs) and heat shock proteins (HSPs) are crucial regulators and effectors of the heat shock response (HSR). In this study, the full-length cDNA sequences of MjHSP10 and MjHSF1 were cloned by rapid amplification of cDNA ends (RACE). The deduced MjHSP10 and MjHSF1 amino acid (aa) sequences exhibited conserved structures and the functional features of HSP10 and HSF1, respectively. The tissue distributions and mRNA expression profiles of the two genes in response to heat stress were analyzed by quantitative real-time PCR (qRT-PCR). MjHSP10 and MjHSF1 were ubiquitously expressed in various tissues. Heat stress induced a significant increase in MjHSP10 expression that tend to positively correlate with temperature. Additionally, MjHSF1 transcription was up-regulated less than MjHSP10 transcription under heat stress. MjHSF1 expression in the hepatopancreas was up-regulated under only long-term (48 h) heat stress, and MjHSF1 transcription in the gill increased under only acute (34 °C) heat stress. MjHSF1 knockdown by RNA interference (RNAi) down-regulated MjHSP10 expression. Glutathione-S-transferase (GST) pull-down assays showed an interaction between MjHSP10 and the DNA-binding domain (DBD) of MjHSF1. This study provided new insights into cross talk between HSP10 and HSF1 in Marsupenaeus japonicus

Identification of two novel C-type lectins involved in immune defense against white spot syndrome virus and Vibrio parahaemolyticus from Marsupenaeus japonicus

Abstract(#br)C-type lectins (CTLs) are vital molecules in crustacean innate immunity with the capacity to recognize and eliminate invaders, such as viruses and bacteria. Here, two novel CTLs were identified from the kuruma shrimp Marsupenaeus japonicus , and their molecular characteristics and immune function were investigated. Sequence analysis revealed that the two CTLs possessed the typical CTL structure and function features. Tissue distribution analysis showed that the two CTLs were most abundantly expressed in the hepatopancreas and weakly expressed in other examined tissues. The transcription of the two CTLs significantly increased in the hepatopancreas of shrimp challenged with both white spot syndrome virus (WSSV) and Vibrio parahaemolyticus , and MjCTL4 was found to be more sensitive to the two pathogens than MjCTL3, being induced at relatively faster and higher increments. GST pull-down assays showed that the two CTLs could directly interact with several WSSV envelope proteins (VP19, VP24, VP26 and VP28). Moreover, the two CTLs displayed obvious binding and antibacterial ability to V. parahaemolyticus , and MjCTL3 exhibited stronger anti- V. parahaemolyticus activity than MjCTL4. These results suggest that the two novel CTLs might function as pattern recognition receptors (PRRs) and antibacterial molecules in M. japonicus innate immunity, and the two CTLs may be alternative agents for the prevention and treatment of diseases caused by WSSV and V. parahaemolyticus

Cross talk between heat shock protein 10 and a heat shock factor identified from Marsupenaeus japonicus.

Heat shock factors (HSFs) and heat shock proteins (HSPs) are crucial regulators and effectors of the heat shock response (HSR). In this study, the full-length cDNA sequences of MjHSP10 and MjHSF1 were cloned by rapid amplification of cDNA ends (RACE). The deduced MjHSP10 and MjHSF1 amino acid (aa) sequences exhibited conserved structures and the functional features of HSP10 and HSF1, respectively. The tissue distributions and mRNA expression profiles of the two genes in response to heat stress were analyzed by quantitative real-time PCR (qRT-PCR). MjHSP10 and MjHSF1 were ubiquitously expressed in various tissues. Heat stress induced a significant increase in MjHSP10 expression that tend to positively correlate with temperature. Additionally, MjHSF1 transcription was up-regulated less than MjHSP10 transcription under heat stress. MjHSF1 expression in the hepatopancreas was up-regulated under only long-term (48 h) heat stress, and MjHSF1 transcription in the gill increased under only acute (34 °C) heat stress. MjHSF1 knockdown by RNA interference (RNAi) down-regulated MjHSP10 expression. Glutathione-S-transferase (GST) pull-down assays showed an interaction between MjHSP10 and the DNA-binding domain (DBD) of MjHSF1. This study provided new insights into cross talk between HSP10 and HSF1 in Marsupenaeus japonicus